Structure, stability, and thermodynamics of a short intermolecular purine-purine-pyrimidine triple helix

We have investigated the structure and physical chemistry of the d(C3T4C3).2[d(G3A4G3)] triple helix by polyacrylamide gel electrophoresis (PAGE), 1H NMR, and ultraviolet (UV) absorption spectroscopy. The triplex was stabilized with MgCl2 at neutral pH. PAGE studies verify the stoichiometry of the s...

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Veröffentlicht in:	Biochemistry (Easton) 1991-06, Vol.30 (25), p.6081-6087
Hauptverfasser:	Pilch, Daniel S, Levenson, Corey, Shafer, Richard H
Format:	Artikel
Sprache:	eng
Schlagworte:	550601 - Medicine- Unsealed Radionuclides in Diagnostics ABSORPTION SPECTROSCOPY BARYONS Base Composition Base Sequence Biological and medical sciences DNA DNA - chemistry ELECTROPHORESIS Electrophoresis, Polyacrylamide Gel ELEMENTARY PARTICLES FERMIONS Fundamental and applied biological sciences. Psychology HADRONS Hydrogen Bonding Interactions. Associations Intermolecular phenomena MAGNETIC RESONANCE Magnetic Resonance Spectroscopy Molecular biophysics Molecular Sequence Data NUCLEAR MAGNETIC RESONANCE Nucleic Acid Conformation NUCLEIC ACID DENATURATION NUCLEIC ACIDS NUCLEONS OLIGONUCLEOTIDES ORGANIC COMPOUNDS PROTONS Purines - chemistry Pyrimidines - chemistry RADIOLOGY AND NUCLEAR MEDICINE RESONANCE SPECTRA SPECTROSCOPY Structure-Activity Relationship THERMAL DEGRADATION Thermodynamics ULTRAVIOLET SPECTRA
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