GLUT5 increases fructose utilization and promotes tumor progression in glioma
Fructose is now such an important component of human diets, and several studies have found that some cancer cells could utilize fructose to overcome low glucose micro-environment, but the study on the role of fructose in glioma is rare. To explore the role of fructose in glioma, we detected the prol...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2018-06, Vol.500 (2), p.462-469 |
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| description | Fructose is now such an important component of human diets, and several studies have found that some cancer cells could utilize fructose to overcome low glucose micro-environment, but the study on the role of fructose in glioma is rare. To explore the role of fructose in glioma, we detected the proliferation and colony formation ability of glioma cells in fructose medium, and found that the abilities of proliferation and colony formation of glioma cells in fructose medium were similar with abilities in glucose medium, however, fructose just partly restored proliferation ability of normal glial cells. To explore the mechanism, we compared the expression level of GLUT5 (Glucose transporter type 5) in these cell lines, and the results showed that glioma cell lines had higher GLUT5 expression than normal glial cell lines. And knockdown of GLUT5 could significantly inhabit cell proliferation of glioma cells in fructose medium. Furthermore, we found that GLUT5 was also higher expressed in glioma tissues, and GLUT5 expression correlated significantly with glioma malignancy and poor survival of glioma patients (p |
| doi_str_mv | 10.1016/j.bbrc.2018.04.103 |
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•Glioma cell lines LN229 and U87 could utilize fructose to maintein survival and proliferation.•Knockdown GLUT5 could inhibit glioma cell proliferation in fructose medium.•GLUT5 was overexpressed in glioma tissues and was correlated with glioma malignancy and patients poor survival.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2018.04.103</identifier><identifier>PMID: 29660339</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; CELL PROLIFERATION ; COLONY FORMATION ; FRUCTOSE ; Glioma ; GLIOMAS ; GLUCOSE ; GLUT5 ; Progression ; PROLIFERATION</subject><ispartof>Biochemical and biophysical research communications, 2018-06, Vol.500 (2), p.462-469</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-373f14f787de84f5045ec95c34d915a984ee7622305a4f43d0496b2a3856623e3</citedby><cites>FETCH-LOGICAL-c384t-373f14f787de84f5045ec95c34d915a984ee7622305a4f43d0496b2a3856623e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2018.04.103$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29660339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/23137108$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Chunhai</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Gao, Wenbo</creatorcontrib><title>GLUT5 increases fructose utilization and promotes tumor progression in glioma</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Fructose is now such an important component of human diets, and several studies have found that some cancer cells could utilize fructose to overcome low glucose micro-environment, but the study on the role of fructose in glioma is rare. To explore the role of fructose in glioma, we detected the proliferation and colony formation ability of glioma cells in fructose medium, and found that the abilities of proliferation and colony formation of glioma cells in fructose medium were similar with abilities in glucose medium, however, fructose just partly restored proliferation ability of normal glial cells. To explore the mechanism, we compared the expression level of GLUT5 (Glucose transporter type 5) in these cell lines, and the results showed that glioma cell lines had higher GLUT5 expression than normal glial cell lines. And knockdown of GLUT5 could significantly inhabit cell proliferation of glioma cells in fructose medium. Furthermore, we found that GLUT5 was also higher expressed in glioma tissues, and GLUT5 expression correlated significantly with glioma malignancy and poor survival of glioma patients (p < 0.01). In addition, we also demonstrated that knockdown of GLUT5 could significantly inhabit tumor proliferation in vivo, and intake fructose could increase tumor volume prominently. Taken together, our data show that fructose can be used by glioma cells, and restrict the fructose intake or targeting GLUT5 could be efficacious strategies in glioma.
•Glioma cell lines LN229 and U87 could utilize fructose to maintein survival and proliferation.•Knockdown GLUT5 could inhibit glioma cell proliferation in fructose medium.•GLUT5 was overexpressed in glioma tissues and was correlated with glioma malignancy and patients poor survival.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>CELL PROLIFERATION</subject><subject>COLONY FORMATION</subject><subject>FRUCTOSE</subject><subject>Glioma</subject><subject>GLIOMAS</subject><subject>GLUCOSE</subject><subject>GLUT5</subject><subject>Progression</subject><subject>PROLIFERATION</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kE1r3DAQhkVoyG7T_oEegqGXXLwdfVi2IJcQ0jSwoZcEehNaebzVYlsbSQ40vz4yu8mxJ6GZZ15eHkK-UVhRoPLHbrXZBLtiQJsViDzjJ2RJQUHJKIhPZAkAsmSK_lmQzzHuACgVUp2RBVNSAudqSR7u1k-PVeFGG9BEjEUXJpt8xGJKrnevJjk_FmZsi33wg0-ZSNPgw_zdBoxxXrux2PbOD-YLOe1MH_Hr8T0nTz9vH29-levfd_c31-vS8kakkte8o6Krm7rFRnQViAqtqiwXraKVUY1ArCVjHCojOsFbEEpumOFNJSXjyM_J90Ouj8npaF1C-9f6cUSbNOOU1xSaTF0eqNz1ecKY9OCixb43I_opagZMitkkyyg7oDb4GAN2eh_cYMI_TUHPiN7pWbaeZWsQecbz0cUxf9oM2H6cvNvNwNUBwOzixWGYq-JosXVhbtp697_8N6hQjrk</recordid><startdate>20180602</startdate><enddate>20180602</enddate><creator>Su, Chunhai</creator><creator>Li, Hui</creator><creator>Gao, Wenbo</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20180602</creationdate><title>GLUT5 increases fructose utilization and promotes tumor progression in glioma</title><author>Su, Chunhai ; Li, Hui ; Gao, Wenbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-373f14f787de84f5045ec95c34d915a984ee7622305a4f43d0496b2a3856623e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>CELL PROLIFERATION</topic><topic>COLONY FORMATION</topic><topic>FRUCTOSE</topic><topic>Glioma</topic><topic>GLIOMAS</topic><topic>GLUCOSE</topic><topic>GLUT5</topic><topic>Progression</topic><topic>PROLIFERATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Chunhai</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Gao, Wenbo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Chunhai</au><au>Li, Hui</au><au>Gao, Wenbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GLUT5 increases fructose utilization and promotes tumor progression in glioma</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2018-06-02</date><risdate>2018</risdate><volume>500</volume><issue>2</issue><spage>462</spage><epage>469</epage><pages>462-469</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Fructose is now such an important component of human diets, and several studies have found that some cancer cells could utilize fructose to overcome low glucose micro-environment, but the study on the role of fructose in glioma is rare. To explore the role of fructose in glioma, we detected the proliferation and colony formation ability of glioma cells in fructose medium, and found that the abilities of proliferation and colony formation of glioma cells in fructose medium were similar with abilities in glucose medium, however, fructose just partly restored proliferation ability of normal glial cells. To explore the mechanism, we compared the expression level of GLUT5 (Glucose transporter type 5) in these cell lines, and the results showed that glioma cell lines had higher GLUT5 expression than normal glial cell lines. And knockdown of GLUT5 could significantly inhabit cell proliferation of glioma cells in fructose medium. Furthermore, we found that GLUT5 was also higher expressed in glioma tissues, and GLUT5 expression correlated significantly with glioma malignancy and poor survival of glioma patients (p < 0.01). In addition, we also demonstrated that knockdown of GLUT5 could significantly inhabit tumor proliferation in vivo, and intake fructose could increase tumor volume prominently. Taken together, our data show that fructose can be used by glioma cells, and restrict the fructose intake or targeting GLUT5 could be efficacious strategies in glioma.
•Glioma cell lines LN229 and U87 could utilize fructose to maintein survival and proliferation.•Knockdown GLUT5 could inhibit glioma cell proliferation in fructose medium.•GLUT5 was overexpressed in glioma tissues and was correlated with glioma malignancy and patients poor survival.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29660339</pmid><doi>10.1016/j.bbrc.2018.04.103</doi><tpages>8</tpages></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | 60 APPLIED LIFE SCIENCES CELL PROLIFERATION COLONY FORMATION FRUCTOSE Glioma GLIOMAS GLUCOSE GLUT5 Progression PROLIFERATION |
| title | GLUT5 increases fructose utilization and promotes tumor progression in glioma |
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