Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis
Arabidopsis amiR:ADC-L2 is a non-lethal line with several developmental defects, it is characterized by a drastic reduction in free polyamine content. Herein, we found that catalase application had growth-promoting effects in amiR:ADC-L2 and parental Ws seedlings. Differences in ROS content between...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2018-12, Vol.506 (4), p.1071-1077 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Ana Isabel, Chávez-Martínez Francisco Ignacio, Jasso-Robles Margarita, Rodríguez-Kessler Gill, Sarvajeet S. Alicia, Becerra-Flora Juan Francisco, Jiménez-Bremont |
| description | Arabidopsis amiR:ADC-L2 is a non-lethal line with several developmental defects, it is characterized by a drastic reduction in free polyamine content. Herein, we found that catalase application had growth-promoting effects in amiR:ADC-L2 and parental Ws seedlings. Differences in ROS content between amiR:ADC-L2 and Ws seedlings were detected. Increased H2O2 levels were found in the amiR:ADC-L2, as well as low AtCAT2 gene expression and reduced catalase activity. Estimation of polyamine oxidase activity in amiR:ADC-L2 line indicated that the over-accumulation of H2O2 is independent of polyamine catabolism. However, increments in NADPH oxidase activity and O2•− content could be associated to the higher H2O2 levels in the amiR:ADC-L2 line. Our data suggest that low polyamine levels in Arabidopsis seedlings are responsible for the accumulation of ROS, by altering the activities of enzymes involved in ROS production and detoxification.
•ROS content and CAT activity is deregulated in a non-lethal line (amiR:ADC-L2) with low polyamine levels.•Catalase exogenous treatment induces a promoting growth in A. thaliana.•Accumulation of ROS in amiR:ADC-L2 line is independent of PA catabolism. |
| doi_str_mv | 10.1016/j.bbrc.2018.10.165 |
| format | Article |
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•ROS content and CAT activity is deregulated in a non-lethal line (amiR:ADC-L2) with low polyamine levels.•Catalase exogenous treatment induces a promoting growth in A. thaliana.•Accumulation of ROS in amiR:ADC-L2 line is independent of PA catabolism.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2018.10.165</identifier><identifier>PMID: 30409429</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ARABIDOPSIS ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; ARGININE ; Arginine decarboxylase ; Carboxy-Lyases - genetics ; Carboxy-Lyases - metabolism ; CATABOLISM ; CATALASE ; Catalase - metabolism ; Catalase activity ; DECARBOXYLASES ; Down-Regulation - genetics ; Gene Expression Regulation, Plant ; Hydrogen Peroxide - metabolism ; NADPH Oxidases - metabolism ; OXIDASES ; Oxidoreductases Acting on CH-NH Group Donors - metabolism ; Plants, Genetically Modified ; Polyamine Oxidase ; Polyamines ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Seedlings - growth & development ; Superoxides - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2018-12, Vol.506 (4), p.1071-1077</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-14b852768bd680f1d89ad0dc0bc935a5d01c6dab401c2154cf184e9b761d06a43</citedby><cites>FETCH-LOGICAL-c384t-14b852768bd680f1d89ad0dc0bc935a5d01c6dab401c2154cf184e9b761d06a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2018.10.165$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30409429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/23136915$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Ana Isabel, Chávez-Martínez</creatorcontrib><creatorcontrib>Francisco Ignacio, Jasso-Robles</creatorcontrib><creatorcontrib>Margarita, Rodríguez-Kessler</creatorcontrib><creatorcontrib>Gill, Sarvajeet S.</creatorcontrib><creatorcontrib>Alicia, Becerra-Flora</creatorcontrib><creatorcontrib>Juan Francisco, Jiménez-Bremont</creatorcontrib><title>Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Arabidopsis amiR:ADC-L2 is a non-lethal line with several developmental defects, it is characterized by a drastic reduction in free polyamine content. Herein, we found that catalase application had growth-promoting effects in amiR:ADC-L2 and parental Ws seedlings. Differences in ROS content between amiR:ADC-L2 and Ws seedlings were detected. Increased H2O2 levels were found in the amiR:ADC-L2, as well as low AtCAT2 gene expression and reduced catalase activity. Estimation of polyamine oxidase activity in amiR:ADC-L2 line indicated that the over-accumulation of H2O2 is independent of polyamine catabolism. However, increments in NADPH oxidase activity and O2•− content could be associated to the higher H2O2 levels in the amiR:ADC-L2 line. Our data suggest that low polyamine levels in Arabidopsis seedlings are responsible for the accumulation of ROS, by altering the activities of enzymes involved in ROS production and detoxification.
•ROS content and CAT activity is deregulated in a non-lethal line (amiR:ADC-L2) with low polyamine levels.•Catalase exogenous treatment induces a promoting growth in A. thaliana.•Accumulation of ROS in amiR:ADC-L2 line is independent of PA catabolism.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ARABIDOPSIS</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>ARGININE</subject><subject>Arginine decarboxylase</subject><subject>Carboxy-Lyases - genetics</subject><subject>Carboxy-Lyases - metabolism</subject><subject>CATABOLISM</subject><subject>CATALASE</subject><subject>Catalase - metabolism</subject><subject>Catalase activity</subject><subject>DECARBOXYLASES</subject><subject>Down-Regulation - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>NADPH Oxidases - metabolism</subject><subject>OXIDASES</subject><subject>Oxidoreductases Acting on CH-NH Group Donors - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Polyamine Oxidase</subject><subject>Polyamines</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Seedlings - growth & development</subject><subject>Superoxides - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFP3DAQha0KVBbaP8ChitRztjOJY2KpFwQtVELiAlJvkT2ebL3ajSM7C8u_b9yFHjm90eh7bzRPiHOEJQKqb-ultZGWFWC7zDvVfBALBA1lhSCPxAIAVFlp_H0iTlNaAyBKpT-KkxokaFnphdhfh-ehjLzabczkw1CEvjBx5Qc_cOGYTLRh_7IxiYsVD1zyfoycUiZn3W2mVPg8Gpr8ExczO2NFGpk8p8IQ7bZvyTN3GY31LozJp0_iuDebxJ9f9Uw8_vzxcHVb3t3f_Lq6vCupbuVUorRtU12o1jrVQo-u1caBI7Ck68Y0DpCUM1bOWmEjqcdWsrYXCh0oI-sz8fWQG9Lku0R-YvpDYRiYpq6qsVYam5mqDhTFkFLkvhuj35r40iF0uexu3eWyu1z2v53Kpi8H07izW3b_LW_tzsD3A8Dzg0-eY77PA7HzMZ93wb-X_xfEgJMB</recordid><startdate>20181202</startdate><enddate>20181202</enddate><creator>Ana Isabel, Chávez-Martínez</creator><creator>Francisco Ignacio, Jasso-Robles</creator><creator>Margarita, Rodríguez-Kessler</creator><creator>Gill, Sarvajeet S.</creator><creator>Alicia, Becerra-Flora</creator><creator>Juan Francisco, Jiménez-Bremont</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>20181202</creationdate><title>Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis</title><author>Ana Isabel, Chávez-Martínez ; Francisco Ignacio, Jasso-Robles ; Margarita, Rodríguez-Kessler ; Gill, Sarvajeet S. ; Alicia, Becerra-Flora ; Juan Francisco, Jiménez-Bremont</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-14b852768bd680f1d89ad0dc0bc935a5d01c6dab401c2154cf184e9b761d06a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ARABIDOPSIS</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>ARGININE</topic><topic>Arginine decarboxylase</topic><topic>Carboxy-Lyases - genetics</topic><topic>Carboxy-Lyases - metabolism</topic><topic>CATABOLISM</topic><topic>CATALASE</topic><topic>Catalase - metabolism</topic><topic>Catalase activity</topic><topic>DECARBOXYLASES</topic><topic>Down-Regulation - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>NADPH Oxidases - metabolism</topic><topic>OXIDASES</topic><topic>Oxidoreductases Acting on CH-NH Group Donors - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Polyamine Oxidase</topic><topic>Polyamines</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Seedlings - growth & development</topic><topic>Superoxides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ana Isabel, Chávez-Martínez</creatorcontrib><creatorcontrib>Francisco Ignacio, Jasso-Robles</creatorcontrib><creatorcontrib>Margarita, Rodríguez-Kessler</creatorcontrib><creatorcontrib>Gill, Sarvajeet S.</creatorcontrib><creatorcontrib>Alicia, Becerra-Flora</creatorcontrib><creatorcontrib>Juan Francisco, Jiménez-Bremont</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ana Isabel, Chávez-Martínez</au><au>Francisco Ignacio, Jasso-Robles</au><au>Margarita, Rodríguez-Kessler</au><au>Gill, Sarvajeet S.</au><au>Alicia, Becerra-Flora</au><au>Juan Francisco, Jiménez-Bremont</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2018-12-02</date><risdate>2018</risdate><volume>506</volume><issue>4</issue><spage>1071</spage><epage>1077</epage><pages>1071-1077</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Arabidopsis amiR:ADC-L2 is a non-lethal line with several developmental defects, it is characterized by a drastic reduction in free polyamine content. Herein, we found that catalase application had growth-promoting effects in amiR:ADC-L2 and parental Ws seedlings. Differences in ROS content between amiR:ADC-L2 and Ws seedlings were detected. Increased H2O2 levels were found in the amiR:ADC-L2, as well as low AtCAT2 gene expression and reduced catalase activity. Estimation of polyamine oxidase activity in amiR:ADC-L2 line indicated that the over-accumulation of H2O2 is independent of polyamine catabolism. However, increments in NADPH oxidase activity and O2•− content could be associated to the higher H2O2 levels in the amiR:ADC-L2 line. Our data suggest that low polyamine levels in Arabidopsis seedlings are responsible for the accumulation of ROS, by altering the activities of enzymes involved in ROS production and detoxification.
•ROS content and CAT activity is deregulated in a non-lethal line (amiR:ADC-L2) with low polyamine levels.•Catalase exogenous treatment induces a promoting growth in A. thaliana.•Accumulation of ROS in amiR:ADC-L2 line is independent of PA catabolism.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30409429</pmid><doi>10.1016/j.bbrc.2018.10.165</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2018-12, Vol.506 (4), p.1071-1077 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 60 APPLIED LIFE SCIENCES ARABIDOPSIS Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism ARGININE Arginine decarboxylase Carboxy-Lyases - genetics Carboxy-Lyases - metabolism CATABOLISM CATALASE Catalase - metabolism Catalase activity DECARBOXYLASES Down-Regulation - genetics Gene Expression Regulation, Plant Hydrogen Peroxide - metabolism NADPH Oxidases - metabolism OXIDASES Oxidoreductases Acting on CH-NH Group Donors - metabolism Plants, Genetically Modified Polyamine Oxidase Polyamines Reactive oxygen species Reactive Oxygen Species - metabolism Seedlings - growth & development Superoxides - metabolism |
| title | Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis |
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