By modulating α2β1 integrin signalling, gastrin increases adhesion oF AGS-GR gastric cancer cells
Peritoneal metastasis is a major cause of recurrence of gastric cancer and integrins are key molecules involved in gastric cancer cells attachment to the peritoneum. The peptide hormone, gastrin, initially identified for its role in gastric acid secretion is also a growth factor for gastric mucosa....
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Veröffentlicht in: | Experimental cell research 2018-01, Vol.362 (2), p.498-503 |
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description | Peritoneal metastasis is a major cause of recurrence of gastric cancer and integrins are key molecules involved in gastric cancer cells attachment to the peritoneum. The peptide hormone, gastrin, initially identified for its role in gastric acid secretion is also a growth factor for gastric mucosa. Gastrin has also been shown to contribute to gastric cancers progression. Here, we provide the first evidence that gastrin increases the adhesion of gastric cancer cells. Gastrin treatment induces the expression of α2 integrin subunit through a mechanism that involves the ERK pathway. We also observed in response to gastrin an increase in the amount of α2 integrin associated with β1subunit. In addition, gastrin-stimulated cell adhesion was blocked with an anti-α2β1 integrin neutralizing antibody. We also show that gastrin activates the integrin pathway via the phosphorylation of β1 integrin by a Src family kinase. This mechanism may contribute to the enhancement of cell adhesion observed in response to gastrin since we found an inhibition of gastrin-mediated cell adhesion when cells were treated with a Src inhibitor. By regulating one of the key step of the metastatic process gastrin might contribute to increase the aggressive behaviour of human gastric tumours.
•The peptide hormone, gastrin up-regulates α2 integrin expression in gastric cancer cells.•Gastrin induces cell adhesion through α2β1 integrin activation.•gastrin activates the integrin pathway via the phosphorylation of β1 by a Src family kinase.•Gastrin may contribute to increase the aggressive behaviour of human gastric tumours. |
doi_str_mv | 10.1016/j.yexcr.2017.12.014 |
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•The peptide hormone, gastrin up-regulates α2 integrin expression in gastric cancer cells.•Gastrin induces cell adhesion through α2β1 integrin activation.•gastrin activates the integrin pathway via the phosphorylation of β1 by a Src family kinase.•Gastrin may contribute to increase the aggressive behaviour of human gastric tumours.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2017.12.014</identifier><identifier>PMID: 29253536</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ANTIBODIES ; Cell adhesion ; GASTRIC ACID ; Gastric cancer ; GASTRIN ; GROWTH FACTORS ; Integrins ; Metastasis ; MUCOUS MEMBRANES ; NEOPLASMS ; PERITONEUM ; PHOSPHOTRANSFERASES</subject><ispartof>Experimental cell research, 2018-01, Vol.362 (2), p.498-503</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-ba5ad51cc155699d1a558bd9c3eb39ce615961d6ad3312f072f1e820c23c588d3</citedby><cites>FETCH-LOGICAL-c302t-ba5ad51cc155699d1a558bd9c3eb39ce615961d6ad3312f072f1e820c23c588d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2017.12.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29253536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/23082502$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kowalski-Chauvel, Aline</creatorcontrib><creatorcontrib>Teissier, Guy</creatorcontrib><creatorcontrib>Toulas, Christine</creatorcontrib><creatorcontrib>Cohen-jonathan-moyal, Elizabeth</creatorcontrib><creatorcontrib>Seva, Catherine</creatorcontrib><title>By modulating α2β1 integrin signalling, gastrin increases adhesion oF AGS-GR gastric cancer cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Peritoneal metastasis is a major cause of recurrence of gastric cancer and integrins are key molecules involved in gastric cancer cells attachment to the peritoneum. The peptide hormone, gastrin, initially identified for its role in gastric acid secretion is also a growth factor for gastric mucosa. Gastrin has also been shown to contribute to gastric cancers progression. Here, we provide the first evidence that gastrin increases the adhesion of gastric cancer cells. Gastrin treatment induces the expression of α2 integrin subunit through a mechanism that involves the ERK pathway. We also observed in response to gastrin an increase in the amount of α2 integrin associated with β1subunit. In addition, gastrin-stimulated cell adhesion was blocked with an anti-α2β1 integrin neutralizing antibody. We also show that gastrin activates the integrin pathway via the phosphorylation of β1 integrin by a Src family kinase. This mechanism may contribute to the enhancement of cell adhesion observed in response to gastrin since we found an inhibition of gastrin-mediated cell adhesion when cells were treated with a Src inhibitor. By regulating one of the key step of the metastatic process gastrin might contribute to increase the aggressive behaviour of human gastric tumours.
•The peptide hormone, gastrin up-regulates α2 integrin expression in gastric cancer cells.•Gastrin induces cell adhesion through α2β1 integrin activation.•gastrin activates the integrin pathway via the phosphorylation of β1 by a Src family kinase.•Gastrin may contribute to increase the aggressive behaviour of human gastric tumours.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ANTIBODIES</subject><subject>Cell adhesion</subject><subject>GASTRIC ACID</subject><subject>Gastric cancer</subject><subject>GASTRIN</subject><subject>GROWTH FACTORS</subject><subject>Integrins</subject><subject>Metastasis</subject><subject>MUCOUS MEMBRANES</subject><subject>NEOPLASMS</subject><subject>PERITONEUM</subject><subject>PHOSPHOTRANSFERASES</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kc9uEzEQxi0EoqHwBEjIEhcO7OKx17veA4dS0YBUCYk_Z8sZT1JHG7vYm4o8FjxIn4ldEjhyGmnmNzOfvo-x5yBqENC-2dYH-oG5lgK6GmQtoHnAFiB6UclGyodsIaZW1RjZnbEnpWyFEMZA-5idyV5qpVW7YPjuwHfJ7wc3hrjh9z_l_S_gIY60ySHyEjbRDcM0es03roxzL0TM5AoV7vwNlZAiT1f8YvmlWn4-QcjRRaTMkYahPGWP1m4o9OxUz9m3q_dfLz9U15-WHy8vritUQo7VymnnNSCC1m3fe3Bam5XvUdFK9Ugt6L4F3zqvFMi16OQayEiBUqE2xqtz9vJ4N5Ux2IJhJLzBFCPhaKUSRmohJ-rVkbrN6fueymh3ocw6XaS0Lxb6znTQmGZG1RHFnErJtLa3OexcPlgQds7Abu2fDOycgQVpJ8OnrRenB_vVjvy_nb-mT8DbI0CTGXeB8qyVJr98yLNUn8J_H_wGzR2ZWg</recordid><startdate>20180115</startdate><enddate>20180115</enddate><creator>Kowalski-Chauvel, Aline</creator><creator>Teissier, Guy</creator><creator>Toulas, Christine</creator><creator>Cohen-jonathan-moyal, Elizabeth</creator><creator>Seva, Catherine</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20180115</creationdate><title>By modulating α2β1 integrin signalling, gastrin increases adhesion oF AGS-GR gastric cancer cells</title><author>Kowalski-Chauvel, Aline ; Teissier, Guy ; Toulas, Christine ; Cohen-jonathan-moyal, Elizabeth ; Seva, Catherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-ba5ad51cc155699d1a558bd9c3eb39ce615961d6ad3312f072f1e820c23c588d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ANTIBODIES</topic><topic>Cell adhesion</topic><topic>GASTRIC ACID</topic><topic>Gastric cancer</topic><topic>GASTRIN</topic><topic>GROWTH FACTORS</topic><topic>Integrins</topic><topic>Metastasis</topic><topic>MUCOUS MEMBRANES</topic><topic>NEOPLASMS</topic><topic>PERITONEUM</topic><topic>PHOSPHOTRANSFERASES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kowalski-Chauvel, Aline</creatorcontrib><creatorcontrib>Teissier, Guy</creatorcontrib><creatorcontrib>Toulas, Christine</creatorcontrib><creatorcontrib>Cohen-jonathan-moyal, Elizabeth</creatorcontrib><creatorcontrib>Seva, Catherine</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kowalski-Chauvel, Aline</au><au>Teissier, Guy</au><au>Toulas, Christine</au><au>Cohen-jonathan-moyal, Elizabeth</au><au>Seva, Catherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>By modulating α2β1 integrin signalling, gastrin increases adhesion oF AGS-GR gastric cancer cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2018-01-15</date><risdate>2018</risdate><volume>362</volume><issue>2</issue><spage>498</spage><epage>503</epage><pages>498-503</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Peritoneal metastasis is a major cause of recurrence of gastric cancer and integrins are key molecules involved in gastric cancer cells attachment to the peritoneum. The peptide hormone, gastrin, initially identified for its role in gastric acid secretion is also a growth factor for gastric mucosa. Gastrin has also been shown to contribute to gastric cancers progression. Here, we provide the first evidence that gastrin increases the adhesion of gastric cancer cells. Gastrin treatment induces the expression of α2 integrin subunit through a mechanism that involves the ERK pathway. We also observed in response to gastrin an increase in the amount of α2 integrin associated with β1subunit. In addition, gastrin-stimulated cell adhesion was blocked with an anti-α2β1 integrin neutralizing antibody. We also show that gastrin activates the integrin pathway via the phosphorylation of β1 integrin by a Src family kinase. This mechanism may contribute to the enhancement of cell adhesion observed in response to gastrin since we found an inhibition of gastrin-mediated cell adhesion when cells were treated with a Src inhibitor. By regulating one of the key step of the metastatic process gastrin might contribute to increase the aggressive behaviour of human gastric tumours.
•The peptide hormone, gastrin up-regulates α2 integrin expression in gastric cancer cells.•Gastrin induces cell adhesion through α2β1 integrin activation.•gastrin activates the integrin pathway via the phosphorylation of β1 by a Src family kinase.•Gastrin may contribute to increase the aggressive behaviour of human gastric tumours.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29253536</pmid><doi>10.1016/j.yexcr.2017.12.014</doi><tpages>6</tpages></addata></record> |
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subjects | 60 APPLIED LIFE SCIENCES ANTIBODIES Cell adhesion GASTRIC ACID Gastric cancer GASTRIN GROWTH FACTORS Integrins Metastasis MUCOUS MEMBRANES NEOPLASMS PERITONEUM PHOSPHOTRANSFERASES |
title | By modulating α2β1 integrin signalling, gastrin increases adhesion oF AGS-GR gastric cancer cells |
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