Improved Stress Tolerance of Saccharomyces cerevisiae by CRISPR-Cas-Mediated Genome Evolution
In bioprocesses, a microorganism with high tolerance to various stresses would be advantageous for efficient bio-based chemical production. Yeast Saccharomyces cerevisiae has long been used in the food industry because of its safety and convenience, and genetically engineered S. cerevisiae strains h...
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Veröffentlicht in: | Applied biochemistry and biotechnology 2019-11, Vol.189 (3), p.810-821 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In bioprocesses, a microorganism with high tolerance to various stresses would be advantageous for efficient bio-based chemical production. Yeast
Saccharomyces cerevisiae
has long been used in the food industry because of its safety and convenience, and genetically engineered
S. cerevisiae
strains have been constructed and used for the production of various bio-based chemicals. In this study, we developed a novel genome shuffling method for
S. cerevisiae
using CRISPR-Cas. By using this, the thermotolerant mutant strain T8-292, which can grow well at 39 °C, was successfully created. The strain also showed higher cell viability in low pH and high ethanol concentration. In addition, the differences in genome structure between mutant and parent strains were suggested by random amplified polymorphic DNA PCR method. Our genome shuffling method could be a promising strategy for improvement of various stress tolerance in
S. cerevisiae
. |
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ISSN: | 0273-2289 1559-0291 |
DOI: | 10.1007/s12010-019-03040-y |