PYK2 mediates BzATP-induced extracellular matrix proteins synthesis
Mechanical stimuli such as fluid shear and cyclic tension force induced extracellular adenosine triphosphate (ATP) release in osteoblasts. In particular, cyclic tension force-induced ATP enhances bone formation through P2X7 activation. Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differ...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2017-12, Vol.494 (3-4), p.663-667 |
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| creator | Torigoe, Go Nagao, Mayu Tanabe, Natsuko Manaka, Soichiro Kariya, Taro Kawato, Takayuki Sekino, Jumpei Kato, Shunichiro Maeno, Masao Suzuki, Naoto Shimizu, Noriyoshi |
| description | Mechanical stimuli such as fluid shear and cyclic tension force induced extracellular adenosine triphosphate (ATP) release in osteoblasts. In particular, cyclic tension force-induced ATP enhances bone formation through P2X7 activation. Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differentiation is induced by mechanical stimuli. Furthermore, activation of PYK2 also was a response to integrin by mechanical stimuli. Extracellular matrix protein (ECMP)s, which are important factors for bone formation are expressed by osteoblasts. However, the effect of the interaction of 2’(3)-Ο-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP), which is the agonist of the mechanosensitive receptor P2X7, with PYK2 on ECMP production is poorly understood. Thus, our purpose was to investigate the effects of PYK2 on BzATP-induced ECMP production in osteoblasts.
BzATP increased phospho-PYK2 protein expression on days 3 and 7 of culture. Furthermore, the PYK2 inhibitor PF431394 inhibited the stimulatory effect of BzATP on the expression of type I collagen, bone sialoprotein and osteocalcin expression. PF431396 did not inhibit the stimulatory effect of BzATP on osteopontin (OPN) mRNA expression.
These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation. |
| doi_str_mv | 10.1016/j.bbrc.2017.10.107 |
| format | Article |
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BzATP increased phospho-PYK2 protein expression on days 3 and 7 of culture. Furthermore, the PYK2 inhibitor PF431394 inhibited the stimulatory effect of BzATP on the expression of type I collagen, bone sialoprotein and osteocalcin expression. PF431396 did not inhibit the stimulatory effect of BzATP on osteopontin (OPN) mRNA expression.
These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2017.10.107</identifier><identifier>PMID: 29061307</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ADENOSINE ; Adenosine Triphosphate - analogs & derivatives ; Adenosine Triphosphate - pharmacology ; Animals ; ATP ; BALB 3T3 Cells ; BIOSYNTHESIS ; BzATP ; COLLAGEN ; CONNECTIVE TISSUE CELLS ; Extracellular Matrix - drug effects ; Extracellular Matrix - physiology ; Extracellular matrix proteins ; Extracellular Matrix Proteins - biosynthesis ; Focal Adhesion Kinase 2 - metabolism ; Mechanical stimuli ; Mechanotransduction, Cellular - genetics ; Mechanotransduction, Cellular - physiology ; MESSENGER-RNA ; Mice ; Osteoblasts - drug effects ; Osteoblasts - physiology ; P2X7 ; PHOSPHOTRANSFERASES ; PROLINE ; PYK2 ; Pyrans - agonists ; Pyrans - metabolism ; RECEPTORS ; SECRETION ; SKELETON ; STIMULI ; TYROSINE</subject><ispartof>Biochemical and biophysical research communications, 2017-12, Vol.494 (3-4), p.663-667</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-df2965ad16b26afbc7a94e83eeb4e12b2cab549267379cb9cf189a508732dffa3</citedby><cites>FETCH-LOGICAL-c450t-df2965ad16b26afbc7a94e83eeb4e12b2cab549267379cb9cf189a508732dffa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2017.10.107$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29061307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22897518$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Torigoe, Go</creatorcontrib><creatorcontrib>Nagao, Mayu</creatorcontrib><creatorcontrib>Tanabe, Natsuko</creatorcontrib><creatorcontrib>Manaka, Soichiro</creatorcontrib><creatorcontrib>Kariya, Taro</creatorcontrib><creatorcontrib>Kawato, Takayuki</creatorcontrib><creatorcontrib>Sekino, Jumpei</creatorcontrib><creatorcontrib>Kato, Shunichiro</creatorcontrib><creatorcontrib>Maeno, Masao</creatorcontrib><creatorcontrib>Suzuki, Naoto</creatorcontrib><creatorcontrib>Shimizu, Noriyoshi</creatorcontrib><title>PYK2 mediates BzATP-induced extracellular matrix proteins synthesis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Mechanical stimuli such as fluid shear and cyclic tension force induced extracellular adenosine triphosphate (ATP) release in osteoblasts. In particular, cyclic tension force-induced ATP enhances bone formation through P2X7 activation. Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differentiation is induced by mechanical stimuli. Furthermore, activation of PYK2 also was a response to integrin by mechanical stimuli. Extracellular matrix protein (ECMP)s, which are important factors for bone formation are expressed by osteoblasts. However, the effect of the interaction of 2’(3)-Ο-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP), which is the agonist of the mechanosensitive receptor P2X7, with PYK2 on ECMP production is poorly understood. Thus, our purpose was to investigate the effects of PYK2 on BzATP-induced ECMP production in osteoblasts.
BzATP increased phospho-PYK2 protein expression on days 3 and 7 of culture. Furthermore, the PYK2 inhibitor PF431394 inhibited the stimulatory effect of BzATP on the expression of type I collagen, bone sialoprotein and osteocalcin expression. PF431396 did not inhibit the stimulatory effect of BzATP on osteopontin (OPN) mRNA expression.
These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ADENOSINE</subject><subject>Adenosine Triphosphate - analogs & derivatives</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>ATP</subject><subject>BALB 3T3 Cells</subject><subject>BIOSYNTHESIS</subject><subject>BzATP</subject><subject>COLLAGEN</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>Extracellular Matrix - drug effects</subject><subject>Extracellular Matrix - physiology</subject><subject>Extracellular matrix proteins</subject><subject>Extracellular Matrix Proteins - biosynthesis</subject><subject>Focal Adhesion Kinase 2 - metabolism</subject><subject>Mechanical stimuli</subject><subject>Mechanotransduction, Cellular - genetics</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>MESSENGER-RNA</subject><subject>Mice</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - physiology</subject><subject>P2X7</subject><subject>PHOSPHOTRANSFERASES</subject><subject>PROLINE</subject><subject>PYK2</subject><subject>Pyrans - agonists</subject><subject>Pyrans - metabolism</subject><subject>RECEPTORS</subject><subject>SECRETION</subject><subject>SKELETON</subject><subject>STIMULI</subject><subject>TYROSINE</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFO3DAQhq0KVBboC_RQReLSS5YZJ7FjiQusWopAggOV2pNlOxPh1W5CbQctPH2TBnrkNNLom1__fIx9RlgioDhdL60NbskB5fLfTn5gCwQFOUco99gCAETOFf46YIcxrgEQS6E-sgOuQGABcsFWd7-vebalxptEMbt4Ob-_y33XDI6ajHYpGEebzbAxIduaFPwuewx9It_FLD536YGij8dsvzWbSJ9e5xH7-f3b_epHfnN7ebU6v8ldWUHKm5YrUZkGheXCtNZJo0qqCyJbEnLLnbFVqbiQhVTOKtdirUwFtSx407amOGInc24fk9fR-UTuwfVdRy5pzmslK6xH6utMjUX_DBST3vo4PWE66oeoUVUViAJLOaJ8Rl3oYwzU6sfgtyY8awQ9KdZrPSnWk-J5Nx19ec0f7Ojt_8mb0xE4mwEaXTx5ClNV6kahPkxNm96_l_8XVj2Mbw</recordid><startdate>20171216</startdate><enddate>20171216</enddate><creator>Torigoe, Go</creator><creator>Nagao, Mayu</creator><creator>Tanabe, Natsuko</creator><creator>Manaka, Soichiro</creator><creator>Kariya, Taro</creator><creator>Kawato, Takayuki</creator><creator>Sekino, Jumpei</creator><creator>Kato, Shunichiro</creator><creator>Maeno, Masao</creator><creator>Suzuki, Naoto</creator><creator>Shimizu, Noriyoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20171216</creationdate><title>PYK2 mediates BzATP-induced extracellular matrix proteins synthesis</title><author>Torigoe, Go ; Nagao, Mayu ; Tanabe, Natsuko ; Manaka, Soichiro ; Kariya, Taro ; Kawato, Takayuki ; Sekino, Jumpei ; Kato, Shunichiro ; Maeno, Masao ; Suzuki, Naoto ; Shimizu, Noriyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-df2965ad16b26afbc7a94e83eeb4e12b2cab549267379cb9cf189a508732dffa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ADENOSINE</topic><topic>Adenosine Triphosphate - analogs & derivatives</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>ATP</topic><topic>BALB 3T3 Cells</topic><topic>BIOSYNTHESIS</topic><topic>BzATP</topic><topic>COLLAGEN</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>Extracellular Matrix - drug effects</topic><topic>Extracellular Matrix - physiology</topic><topic>Extracellular matrix proteins</topic><topic>Extracellular Matrix Proteins - biosynthesis</topic><topic>Focal Adhesion Kinase 2 - metabolism</topic><topic>Mechanical stimuli</topic><topic>Mechanotransduction, Cellular - genetics</topic><topic>Mechanotransduction, Cellular - physiology</topic><topic>MESSENGER-RNA</topic><topic>Mice</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - physiology</topic><topic>P2X7</topic><topic>PHOSPHOTRANSFERASES</topic><topic>PROLINE</topic><topic>PYK2</topic><topic>Pyrans - agonists</topic><topic>Pyrans - metabolism</topic><topic>RECEPTORS</topic><topic>SECRETION</topic><topic>SKELETON</topic><topic>STIMULI</topic><topic>TYROSINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torigoe, Go</creatorcontrib><creatorcontrib>Nagao, Mayu</creatorcontrib><creatorcontrib>Tanabe, Natsuko</creatorcontrib><creatorcontrib>Manaka, Soichiro</creatorcontrib><creatorcontrib>Kariya, Taro</creatorcontrib><creatorcontrib>Kawato, Takayuki</creatorcontrib><creatorcontrib>Sekino, Jumpei</creatorcontrib><creatorcontrib>Kato, Shunichiro</creatorcontrib><creatorcontrib>Maeno, Masao</creatorcontrib><creatorcontrib>Suzuki, Naoto</creatorcontrib><creatorcontrib>Shimizu, Noriyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torigoe, Go</au><au>Nagao, Mayu</au><au>Tanabe, Natsuko</au><au>Manaka, Soichiro</au><au>Kariya, Taro</au><au>Kawato, Takayuki</au><au>Sekino, Jumpei</au><au>Kato, Shunichiro</au><au>Maeno, Masao</au><au>Suzuki, Naoto</au><au>Shimizu, Noriyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PYK2 mediates BzATP-induced extracellular matrix proteins synthesis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2017-12-16</date><risdate>2017</risdate><volume>494</volume><issue>3-4</issue><spage>663</spage><epage>667</epage><pages>663-667</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Mechanical stimuli such as fluid shear and cyclic tension force induced extracellular adenosine triphosphate (ATP) release in osteoblasts. In particular, cyclic tension force-induced ATP enhances bone formation through P2X7 activation. Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differentiation is induced by mechanical stimuli. Furthermore, activation of PYK2 also was a response to integrin by mechanical stimuli. Extracellular matrix protein (ECMP)s, which are important factors for bone formation are expressed by osteoblasts. However, the effect of the interaction of 2’(3)-Ο-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP), which is the agonist of the mechanosensitive receptor P2X7, with PYK2 on ECMP production is poorly understood. Thus, our purpose was to investigate the effects of PYK2 on BzATP-induced ECMP production in osteoblasts.
BzATP increased phospho-PYK2 protein expression on days 3 and 7 of culture. Furthermore, the PYK2 inhibitor PF431394 inhibited the stimulatory effect of BzATP on the expression of type I collagen, bone sialoprotein and osteocalcin expression. PF431396 did not inhibit the stimulatory effect of BzATP on osteopontin (OPN) mRNA expression.
These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29061307</pmid><doi>10.1016/j.bbrc.2017.10.107</doi><tpages>5</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES ADENOSINE Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - pharmacology Animals ATP BALB 3T3 Cells BIOSYNTHESIS BzATP COLLAGEN CONNECTIVE TISSUE CELLS Extracellular Matrix - drug effects Extracellular Matrix - physiology Extracellular matrix proteins Extracellular Matrix Proteins - biosynthesis Focal Adhesion Kinase 2 - metabolism Mechanical stimuli Mechanotransduction, Cellular - genetics Mechanotransduction, Cellular - physiology MESSENGER-RNA Mice Osteoblasts - drug effects Osteoblasts - physiology P2X7 PHOSPHOTRANSFERASES PROLINE PYK2 Pyrans - agonists Pyrans - metabolism RECEPTORS SECRETION SKELETON STIMULI TYROSINE |
| title | PYK2 mediates BzATP-induced extracellular matrix proteins synthesis |
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