Antioxidant capacity automatic assay based on inline photogenerated radical species from l-glutathione-capped CdTe quantum dots
This work aimed at the development of a methodology implemented in an automatic flow system for determination of the antioxidant capacity in food samples, based on the luminol oxidation by inline photogenerated radical species from cadmium telluride nanoparticles capped with l-glutathione. Radical s...
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| Veröffentlicht in: | Talanta (Oxford) 2015-08, Vol.141 (C), p.220-229 |
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| creator | Rodrigues, Daniela M.C. Ribeiro, David S.M. Frigerio, Christian Rodrigues, S. Sofia M. Santos, João L.M. Prior, João A.V. |
| description | This work aimed at the development of a methodology implemented in an automatic flow system for determination of the antioxidant capacity in food samples, based on the luminol oxidation by inline photogenerated radical species from cadmium telluride nanoparticles capped with l-glutathione. Radical species were generated inline by a high-power visible light obtained by Light Emitting Diodes (LEDs) assembled in a multipumping flow system (MPFS). The use of visible light instead of UV radiation allowed the development of a new methodology for antioxidant capacity determination, more environment friendly and to circumvent the risk for UV photo-induced degradation of sample antioxidant compounds. Additionally, the formation of superoxide radical species was theoretically predicted considering the variation of the redox potential with the size of CdTe QDs and the values of redox potential of the oxidizing and oxidable species present in the irradiated medium.
The obtained results of trolox equivalent antioxidant capacity (TEAC) from the analysis of commercial beverages were compared with the results of ABTS and DPPH batch assays through Spearman's-Rho correlation coefficients and no correlation was found (for ABTS: ρ=0.2, p |
| doi_str_mv | 10.1016/j.talanta.2015.04.013 |
| format | Article |
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The obtained results of trolox equivalent antioxidant capacity (TEAC) from the analysis of commercial beverages were compared with the results of ABTS and DPPH batch assays through Spearman's-Rho correlation coefficients and no correlation was found (for ABTS: ρ=0.2, p<0.6 and for DPPH: ρ=0.5, p<0.1) since the mechanism of action of the proposed methodology was based on the scavenging capacity of ROS species rather than the reduction of a colored oxidant.
An analytical linear response range between 0.0001 and 0.005mmolL−1 of trolox and a limit of detection of 0.00005mmolL−1 was found. The QDs based MPFS methodology allowed a determination rate of about 79h−1, a total waste generation of 20.5mLh−1 and the consumption of 0.100mgh−1 of QDs and 2.1mgh−1 of luminol.
[Display omitted]
•CdTe QDs 3.4nm generate O2●− radical species upon exposure to visible radiation.•A high power visible light LED was used for the ROS inline generation, in a MPFS.•Radical oxygen species oxidize luminol producing chemiluminescence emission.•Antioxidants scavenge radical species quenching chemiluminescence emission.•Antioxidant capacity determined by the antioxidants scavenging of ROS.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2015.04.013</identifier><identifier>PMID: 25966406</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antioxidant capacity ; Light Emitting Diode ; Multipumping ; Quantum dots ; Radicals photogeneration ; Superoxide</subject><ispartof>Talanta (Oxford), 2015-08, Vol.141 (C), p.220-229</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-6eeb1cffdd045f7f524447023d4c0acb1c18c0bec063260aa4c28952226e79f93</citedby><cites>FETCH-LOGICAL-c439t-6eeb1cffdd045f7f524447023d4c0acb1c18c0bec063260aa4c28952226e79f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0039914015002519$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25966406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/2280346$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodrigues, Daniela M.C.</creatorcontrib><creatorcontrib>Ribeiro, David S.M.</creatorcontrib><creatorcontrib>Frigerio, Christian</creatorcontrib><creatorcontrib>Rodrigues, S. Sofia M.</creatorcontrib><creatorcontrib>Santos, João L.M.</creatorcontrib><creatorcontrib>Prior, João A.V.</creatorcontrib><title>Antioxidant capacity automatic assay based on inline photogenerated radical species from l-glutathione-capped CdTe quantum dots</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>This work aimed at the development of a methodology implemented in an automatic flow system for determination of the antioxidant capacity in food samples, based on the luminol oxidation by inline photogenerated radical species from cadmium telluride nanoparticles capped with l-glutathione. Radical species were generated inline by a high-power visible light obtained by Light Emitting Diodes (LEDs) assembled in a multipumping flow system (MPFS). The use of visible light instead of UV radiation allowed the development of a new methodology for antioxidant capacity determination, more environment friendly and to circumvent the risk for UV photo-induced degradation of sample antioxidant compounds. Additionally, the formation of superoxide radical species was theoretically predicted considering the variation of the redox potential with the size of CdTe QDs and the values of redox potential of the oxidizing and oxidable species present in the irradiated medium.
The obtained results of trolox equivalent antioxidant capacity (TEAC) from the analysis of commercial beverages were compared with the results of ABTS and DPPH batch assays through Spearman's-Rho correlation coefficients and no correlation was found (for ABTS: ρ=0.2, p<0.6 and for DPPH: ρ=0.5, p<0.1) since the mechanism of action of the proposed methodology was based on the scavenging capacity of ROS species rather than the reduction of a colored oxidant.
An analytical linear response range between 0.0001 and 0.005mmolL−1 of trolox and a limit of detection of 0.00005mmolL−1 was found. The QDs based MPFS methodology allowed a determination rate of about 79h−1, a total waste generation of 20.5mLh−1 and the consumption of 0.100mgh−1 of QDs and 2.1mgh−1 of luminol.
[Display omitted]
•CdTe QDs 3.4nm generate O2●− radical species upon exposure to visible radiation.•A high power visible light LED was used for the ROS inline generation, in a MPFS.•Radical oxygen species oxidize luminol producing chemiluminescence emission.•Antioxidants scavenge radical species quenching chemiluminescence emission.•Antioxidant capacity determined by the antioxidants scavenging of ROS.</description><subject>Antioxidant capacity</subject><subject>Light Emitting Diode</subject><subject>Multipumping</subject><subject>Quantum dots</subject><subject>Radicals photogeneration</subject><subject>Superoxide</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxSMEotvCRwBZnLgkjP_Em5xQtQKKVIlLOVtee9L1KrFT20HdE18dR7tw5TSH-c17M_Oq6h2FhgKVn45N1qP2WTcMaNuAaIDyF9WGdlte83bLX1YbAN7XPRVwVV2ndAQAxoG_rq5Y20spQG6q37c-u_DsbJEiRs_auHwieslh0tkZolPSJ7LXCS0Jnjg_Oo9kPoQcHtFj1Lk0orbO6JGkGY3DRIYYJjLWj-OSdT644LEu0nMhd_YBydNSzJaJ2JDTm-rVoMeEby_1pvr59cvD7q6-__Ht--72vjaC97mWiHtqhsFaEO2wHVomhNiWc6wwoE3p0c7AHg1IziRoLQzr-pYxJnHbDz2_qT6cdUPKTqVyJZqDCd6jyYqxDriQBfp4huYYnhZMWU0uGRzLnzEsSVHZQd8K0a167Rk1MaQUcVBzdJOOJ0VBrQGpo7oEpNaAFAhVAipz7y8Wy35C-2_qbyIF-HwGsHzjl8O4LoveoHVx3dUG9x-LP17tpn8</recordid><startdate>20150815</startdate><enddate>20150815</enddate><creator>Rodrigues, Daniela M.C.</creator><creator>Ribeiro, David S.M.</creator><creator>Frigerio, Christian</creator><creator>Rodrigues, S. Sofia M.</creator><creator>Santos, João L.M.</creator><creator>Prior, João A.V.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20150815</creationdate><title>Antioxidant capacity automatic assay based on inline photogenerated radical species from l-glutathione-capped CdTe quantum dots</title><author>Rodrigues, Daniela M.C. ; Ribeiro, David S.M. ; Frigerio, Christian ; Rodrigues, S. Sofia M. ; Santos, João L.M. ; Prior, João A.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-6eeb1cffdd045f7f524447023d4c0acb1c18c0bec063260aa4c28952226e79f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antioxidant capacity</topic><topic>Light Emitting Diode</topic><topic>Multipumping</topic><topic>Quantum dots</topic><topic>Radicals photogeneration</topic><topic>Superoxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, Daniela M.C.</creatorcontrib><creatorcontrib>Ribeiro, David S.M.</creatorcontrib><creatorcontrib>Frigerio, Christian</creatorcontrib><creatorcontrib>Rodrigues, S. Sofia M.</creatorcontrib><creatorcontrib>Santos, João L.M.</creatorcontrib><creatorcontrib>Prior, João A.V.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodrigues, Daniela M.C.</au><au>Ribeiro, David S.M.</au><au>Frigerio, Christian</au><au>Rodrigues, S. Sofia M.</au><au>Santos, João L.M.</au><au>Prior, João A.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant capacity automatic assay based on inline photogenerated radical species from l-glutathione-capped CdTe quantum dots</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2015-08-15</date><risdate>2015</risdate><volume>141</volume><issue>C</issue><spage>220</spage><epage>229</epage><pages>220-229</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>This work aimed at the development of a methodology implemented in an automatic flow system for determination of the antioxidant capacity in food samples, based on the luminol oxidation by inline photogenerated radical species from cadmium telluride nanoparticles capped with l-glutathione. Radical species were generated inline by a high-power visible light obtained by Light Emitting Diodes (LEDs) assembled in a multipumping flow system (MPFS). The use of visible light instead of UV radiation allowed the development of a new methodology for antioxidant capacity determination, more environment friendly and to circumvent the risk for UV photo-induced degradation of sample antioxidant compounds. Additionally, the formation of superoxide radical species was theoretically predicted considering the variation of the redox potential with the size of CdTe QDs and the values of redox potential of the oxidizing and oxidable species present in the irradiated medium.
The obtained results of trolox equivalent antioxidant capacity (TEAC) from the analysis of commercial beverages were compared with the results of ABTS and DPPH batch assays through Spearman's-Rho correlation coefficients and no correlation was found (for ABTS: ρ=0.2, p<0.6 and for DPPH: ρ=0.5, p<0.1) since the mechanism of action of the proposed methodology was based on the scavenging capacity of ROS species rather than the reduction of a colored oxidant.
An analytical linear response range between 0.0001 and 0.005mmolL−1 of trolox and a limit of detection of 0.00005mmolL−1 was found. The QDs based MPFS methodology allowed a determination rate of about 79h−1, a total waste generation of 20.5mLh−1 and the consumption of 0.100mgh−1 of QDs and 2.1mgh−1 of luminol.
[Display omitted]
•CdTe QDs 3.4nm generate O2●− radical species upon exposure to visible radiation.•A high power visible light LED was used for the ROS inline generation, in a MPFS.•Radical oxygen species oxidize luminol producing chemiluminescence emission.•Antioxidants scavenge radical species quenching chemiluminescence emission.•Antioxidant capacity determined by the antioxidants scavenging of ROS.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25966406</pmid><doi>10.1016/j.talanta.2015.04.013</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antioxidant capacity Light Emitting Diode Multipumping Quantum dots Radicals photogeneration Superoxide |
| title | Antioxidant capacity automatic assay based on inline photogenerated radical species from l-glutathione-capped CdTe quantum dots |
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