Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway
Taxifolin, a flavonoid compound, has been reported to stimulate osteogenic differentiation in osteoblasts. The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The prolifera...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2017-08, Vol.490 (1), p.36-43 |
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| creator | Wang, Yun-Jia Zhang, Hong-Qi Han, Hai-Long Zou, Yong-Yi Gao, Qi-Le Yang, Guan-Teng |
| description | Taxifolin, a flavonoid compound, has been reported to stimulate osteogenic differentiation in osteoblasts. The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The proliferation and osteogenic differentiation of hBMSCs in the presence of taxifolin were examined by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining and Alizarin red staining. The expression of osteogenic differentiation markers were detected by real-time quantitative PCR (RT-PCR) analysis and western blot assay. The activation of potential related pathways was examined by luciferase reporter assay, immunofluorescence and western blot analysis. Taxifolin treatment increased osteogenic differentiation of hBMSCs without cytotoxicity. Luciferase reporter assay showed that taxifolin could not activate estrogen receptor pathway, but inhibit TNF-α-induced NF-κB signaling pathway activation in osteogenic induction condition. Moreover, the nucleus translocation of NF-κB under TNF-α treatment was inhibited by taxifolin treatment. The taxifolin-induced osteogenic differentiation effects of hBMSCs were abolished by TNF-α treatment. In conclusion, our results suggested that taxifolin could promote osteogenesis of hBMSCs, partially through antagonism of NF-κB signaling pathway. |
| doi_str_mv | 10.1016/j.bbrc.2017.06.002 |
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The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The proliferation and osteogenic differentiation of hBMSCs in the presence of taxifolin were examined by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining and Alizarin red staining. The expression of osteogenic differentiation markers were detected by real-time quantitative PCR (RT-PCR) analysis and western blot assay. The activation of potential related pathways was examined by luciferase reporter assay, immunofluorescence and western blot analysis. Taxifolin treatment increased osteogenic differentiation of hBMSCs without cytotoxicity. Luciferase reporter assay showed that taxifolin could not activate estrogen receptor pathway, but inhibit TNF-α-induced NF-κB signaling pathway activation in osteogenic induction condition. Moreover, the nucleus translocation of NF-κB under TNF-α treatment was inhibited by taxifolin treatment. The taxifolin-induced osteogenic differentiation effects of hBMSCs were abolished by TNF-α treatment. In conclusion, our results suggested that taxifolin could promote osteogenesis of hBMSCs, partially through antagonism of NF-κB signaling pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2017.06.002</identifier><identifier>PMID: 28579433</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ALIZARIN ; ALKALINE PHOSPHATASE ; BONE MARROW ; Cell Differentiation - drug effects ; Cells, Cultured ; CONNECTIVE TISSUE CELLS ; DMSO ; Dose-Response Relationship, Drug ; ESTROGENS ; Flavonoid ; FLAVONOIDS ; Humans ; LUCIFERASE ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - metabolism ; MITOGENS ; Molecular Structure ; MSC ; NF-kappa B - metabolism ; NF-κB ; Osteogenesis ; Osteogenesis - drug effects ; PHOSPHOTRANSFERASES ; POLYMERASE CHAIN REACTION ; Protein Transport - drug effects ; Quercetin - analogs & derivatives ; Quercetin - pharmacology ; RECEPTORS ; Signal Transduction - drug effects ; SKELETON ; STEM CELLS ; Structure-Activity Relationship ; Taxifolin ; TOXICITY ; TRANSCRIPTION ; TRANSCRIPTION FACTORS ; TRANSLOCATION</subject><ispartof>Biochemical and biophysical research communications, 2017-08, Vol.490 (1), p.36-43</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-6af512abfc1c8ee06bb105d3c6f423d7a4f02969f040e5cdc5fbd4dad61f643b3</citedby><cites>FETCH-LOGICAL-c384t-6af512abfc1c8ee06bb105d3c6f423d7a4f02969f040e5cdc5fbd4dad61f643b3</cites><orcidid>0000-0001-5750-4910 ; 0000-0003-4691-074X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2017.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28579433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22719027$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yun-Jia</creatorcontrib><creatorcontrib>Zhang, Hong-Qi</creatorcontrib><creatorcontrib>Han, Hai-Long</creatorcontrib><creatorcontrib>Zou, Yong-Yi</creatorcontrib><creatorcontrib>Gao, Qi-Le</creatorcontrib><creatorcontrib>Yang, Guan-Teng</creatorcontrib><title>Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Taxifolin, a flavonoid compound, has been reported to stimulate osteogenic differentiation in osteoblasts. The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The proliferation and osteogenic differentiation of hBMSCs in the presence of taxifolin were examined by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining and Alizarin red staining. The expression of osteogenic differentiation markers were detected by real-time quantitative PCR (RT-PCR) analysis and western blot assay. The activation of potential related pathways was examined by luciferase reporter assay, immunofluorescence and western blot analysis. Taxifolin treatment increased osteogenic differentiation of hBMSCs without cytotoxicity. Luciferase reporter assay showed that taxifolin could not activate estrogen receptor pathway, but inhibit TNF-α-induced NF-κB signaling pathway activation in osteogenic induction condition. Moreover, the nucleus translocation of NF-κB under TNF-α treatment was inhibited by taxifolin treatment. The taxifolin-induced osteogenic differentiation effects of hBMSCs were abolished by TNF-α treatment. In conclusion, our results suggested that taxifolin could promote osteogenesis of hBMSCs, partially through antagonism of NF-κB signaling pathway.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ALIZARIN</subject><subject>ALKALINE PHOSPHATASE</subject><subject>BONE MARROW</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>DMSO</subject><subject>Dose-Response Relationship, Drug</subject><subject>ESTROGENS</subject><subject>Flavonoid</subject><subject>FLAVONOIDS</subject><subject>Humans</subject><subject>LUCIFERASE</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>MITOGENS</subject><subject>Molecular Structure</subject><subject>MSC</subject><subject>NF-kappa B - metabolism</subject><subject>NF-κB</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>PHOSPHOTRANSFERASES</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Protein Transport - drug effects</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - pharmacology</subject><subject>RECEPTORS</subject><subject>Signal Transduction - drug effects</subject><subject>SKELETON</subject><subject>STEM CELLS</subject><subject>Structure-Activity Relationship</subject><subject>Taxifolin</subject><subject>TOXICITY</subject><subject>TRANSCRIPTION</subject><subject>TRANSCRIPTION FACTORS</subject><subject>TRANSLOCATION</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGO1DAQRS0EYpqBC7BAltiwSSg7jtOR2MCIAaQRbAaJneXYZeJWYjd2uoe-GofgTDjqgSWrkkrvf1X9T8hzBjUDJl_v6mFIpubAuhpkDcAfkA2DHirOQDwkGwCQFe_ZtwvyJOcdAGNC9o_JBd-2XS-aZkOOt_qnd3HygWIYdTCYacwLxu8YvKHWO4cJw-L14mOg0dHxMOtAhxiQzjqleEdnzBjMeJr1RIt0pganKdO9TkU2TSd69Jp-vq5-_3pXlst4p09PySOnp4zP7ucl-Xr9_vbqY3Xz5cOnq7c3lWm2Yqmkdi3jenCGmS0iyGFg0NrGSCd4YzstHPBe9g4EYGusad1ghdVWMidFMzSX5OXZt_zkVTZ-QTOaGAKaRXHesR54V6hXZ2qf4o8D5kXNPq9P6IDxkFWhJBNtJ5uC8jNqUsw5oVP75EsOJ8VAra2onVpbUWsrCqQqrRTRi3v_wzCj_Sf5W0MB3pwBLFkcPab11JIpWp_WS230__P_A1N7oO8</recordid><startdate>20170812</startdate><enddate>20170812</enddate><creator>Wang, Yun-Jia</creator><creator>Zhang, Hong-Qi</creator><creator>Han, Hai-Long</creator><creator>Zou, Yong-Yi</creator><creator>Gao, Qi-Le</creator><creator>Yang, Guan-Teng</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0001-5750-4910</orcidid><orcidid>https://orcid.org/0000-0003-4691-074X</orcidid></search><sort><creationdate>20170812</creationdate><title>Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway</title><author>Wang, Yun-Jia ; Zhang, Hong-Qi ; Han, Hai-Long ; Zou, Yong-Yi ; Gao, Qi-Le ; Yang, Guan-Teng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-6af512abfc1c8ee06bb105d3c6f423d7a4f02969f040e5cdc5fbd4dad61f643b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ALIZARIN</topic><topic>ALKALINE PHOSPHATASE</topic><topic>BONE MARROW</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>DMSO</topic><topic>Dose-Response Relationship, Drug</topic><topic>ESTROGENS</topic><topic>Flavonoid</topic><topic>FLAVONOIDS</topic><topic>Humans</topic><topic>LUCIFERASE</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>MITOGENS</topic><topic>Molecular Structure</topic><topic>MSC</topic><topic>NF-kappa B - metabolism</topic><topic>NF-κB</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>PHOSPHOTRANSFERASES</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Protein Transport - drug effects</topic><topic>Quercetin - analogs & derivatives</topic><topic>Quercetin - pharmacology</topic><topic>RECEPTORS</topic><topic>Signal Transduction - drug effects</topic><topic>SKELETON</topic><topic>STEM CELLS</topic><topic>Structure-Activity Relationship</topic><topic>Taxifolin</topic><topic>TOXICITY</topic><topic>TRANSCRIPTION</topic><topic>TRANSCRIPTION FACTORS</topic><topic>TRANSLOCATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yun-Jia</creatorcontrib><creatorcontrib>Zhang, Hong-Qi</creatorcontrib><creatorcontrib>Han, Hai-Long</creatorcontrib><creatorcontrib>Zou, Yong-Yi</creatorcontrib><creatorcontrib>Gao, Qi-Le</creatorcontrib><creatorcontrib>Yang, Guan-Teng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yun-Jia</au><au>Zhang, Hong-Qi</au><au>Han, Hai-Long</au><au>Zou, Yong-Yi</au><au>Gao, Qi-Le</au><au>Yang, Guan-Teng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2017-08-12</date><risdate>2017</risdate><volume>490</volume><issue>1</issue><spage>36</spage><epage>43</epage><pages>36-43</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Taxifolin, a flavonoid compound, has been reported to stimulate osteogenic differentiation in osteoblasts. The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The proliferation and osteogenic differentiation of hBMSCs in the presence of taxifolin were examined by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining and Alizarin red staining. The expression of osteogenic differentiation markers were detected by real-time quantitative PCR (RT-PCR) analysis and western blot assay. The activation of potential related pathways was examined by luciferase reporter assay, immunofluorescence and western blot analysis. Taxifolin treatment increased osteogenic differentiation of hBMSCs without cytotoxicity. Luciferase reporter assay showed that taxifolin could not activate estrogen receptor pathway, but inhibit TNF-α-induced NF-κB signaling pathway activation in osteogenic induction condition. Moreover, the nucleus translocation of NF-κB under TNF-α treatment was inhibited by taxifolin treatment. The taxifolin-induced osteogenic differentiation effects of hBMSCs were abolished by TNF-α treatment. In conclusion, our results suggested that taxifolin could promote osteogenesis of hBMSCs, partially through antagonism of NF-κB signaling pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28579433</pmid><doi>10.1016/j.bbrc.2017.06.002</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5750-4910</orcidid><orcidid>https://orcid.org/0000-0003-4691-074X</orcidid></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES ALIZARIN ALKALINE PHOSPHATASE BONE MARROW Cell Differentiation - drug effects Cells, Cultured CONNECTIVE TISSUE CELLS DMSO Dose-Response Relationship, Drug ESTROGENS Flavonoid FLAVONOIDS Humans LUCIFERASE Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - metabolism MITOGENS Molecular Structure MSC NF-kappa B - metabolism NF-κB Osteogenesis Osteogenesis - drug effects PHOSPHOTRANSFERASES POLYMERASE CHAIN REACTION Protein Transport - drug effects Quercetin - analogs & derivatives Quercetin - pharmacology RECEPTORS Signal Transduction - drug effects SKELETON STEM CELLS Structure-Activity Relationship Taxifolin TOXICITY TRANSCRIPTION TRANSCRIPTION FACTORS TRANSLOCATION |
| title | Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway |
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