Isochoric and isobaric freezing of fish muscle

We have recently shown that, a living organism, which succumbs to freezing to −4 °C in an isobaric thermodynamic system (constant atmospheric pressure), can survive freezing to −4 °C in an isochoric thermodynamic system (constant volume). It is known that the mechanism of cell damage in an isobaric...

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Veröffentlicht in:Biochemical and biophysical research communications 2017-04, Vol.485 (2), p.279-283
Hauptverfasser: Năstase, Gabriel, Lyu, Chenang, Ukpai, Gideon, Şerban, Alexandru, Rubinsky, Boris
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container_issue 2
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creator Năstase, Gabriel
Lyu, Chenang
Ukpai, Gideon
Şerban, Alexandru
Rubinsky, Boris
description We have recently shown that, a living organism, which succumbs to freezing to −4 °C in an isobaric thermodynamic system (constant atmospheric pressure), can survive freezing to −4 °C in an isochoric thermodynamic system (constant volume). It is known that the mechanism of cell damage in an isobaric system is the freezing caused increase in extracellular osmolality, and, the consequent cell dehydration. An explanation for the observed survival during isochoric freezing is the thermodynamic modeling supported hypothesis that, in the isochoric frozen solution the extracellular osmolality is comparable to the cell intracellular osmolality. Therefore, cells in the isochoric frozen organism do not dehydrate, and the tissue maintains its morphological integrity. Comparing the histology of: a) fresh fish white muscle, b) fresh muscle frozen to −5 °C in an isobaric system and c) fresh muscle frozen to −5 °C I in an isochoric system, we find convincing evidence of the mechanism of cell dehydration during isobaric freezing. In contrast, the muscle tissue frozen to −5 °C in an isochoric system appears morphologically identical to fresh tissue, with no evidence of dehydration. This is the first experimental evidence in support of the hypothesis that in isochoric freezing there is no cellular dehydration and therefore the morphology of the frozen tissue remains intact. •Preservation of fish muscle at, subfreezing temperatures, in an isochoric system, is demonstrated.•Experiments were performed to an average pressure of 41.3 MPa and temperatures of −5 °C.•Isochoric subfreezing temperature is a new preservation method that does not require the.use of cryoprotectants.•No cellular dehydration and therefore the morphology of the frozen tissue remains intact.
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It is known that the mechanism of cell damage in an isobaric system is the freezing caused increase in extracellular osmolality, and, the consequent cell dehydration. An explanation for the observed survival during isochoric freezing is the thermodynamic modeling supported hypothesis that, in the isochoric frozen solution the extracellular osmolality is comparable to the cell intracellular osmolality. Therefore, cells in the isochoric frozen organism do not dehydrate, and the tissue maintains its morphological integrity. Comparing the histology of: a) fresh fish white muscle, b) fresh muscle frozen to −5 °C in an isobaric system and c) fresh muscle frozen to −5 °C I in an isochoric system, we find convincing evidence of the mechanism of cell dehydration during isobaric freezing. In contrast, the muscle tissue frozen to −5 °C in an isochoric system appears morphologically identical to fresh tissue, with no evidence of dehydration. 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It is known that the mechanism of cell damage in an isobaric system is the freezing caused increase in extracellular osmolality, and, the consequent cell dehydration. An explanation for the observed survival during isochoric freezing is the thermodynamic modeling supported hypothesis that, in the isochoric frozen solution the extracellular osmolality is comparable to the cell intracellular osmolality. Therefore, cells in the isochoric frozen organism do not dehydrate, and the tissue maintains its morphological integrity. Comparing the histology of: a) fresh fish white muscle, b) fresh muscle frozen to −5 °C in an isobaric system and c) fresh muscle frozen to −5 °C I in an isochoric system, we find convincing evidence of the mechanism of cell dehydration during isobaric freezing. In contrast, the muscle tissue frozen to −5 °C in an isochoric system appears morphologically identical to fresh tissue, with no evidence of dehydration. This is the first experimental evidence in support of the hypothesis that in isochoric freezing there is no cellular dehydration and therefore the morphology of the frozen tissue remains intact. •Preservation of fish muscle at, subfreezing temperatures, in an isochoric system, is demonstrated.•Experiments were performed to an average pressure of 41.3 MPa and temperatures of −5 °C.•Isochoric subfreezing temperature is a new preservation method that does not require the.use of cryoprotectants.•No cellular dehydration and therefore the morphology of the frozen tissue remains intact.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>Atmospheric Pressure</subject><subject>Blue Tilapia</subject><subject>Cell Survival</subject><subject>Cryopreservation - methods</subject><subject>DEHYDRATION</subject><subject>Fish muscle</subject><subject>FREEZING</subject><subject>Histology</subject><subject>Isobaric</subject><subject>Isochoric</subject><subject>MORPHOLOGY</subject><subject>MUSCLES</subject><subject>Muscles - cytology</subject><subject>Muscles - ultrastructure</subject><subject>Osmolar Concentration</subject><subject>PLANT TISSUES</subject><subject>Preservation</subject><subject>THERMODYNAMICS</subject><subject>Tilapia - anatomy &amp; 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subjects 60 APPLIED LIFE SCIENCES
ANIMAL TISSUES
Animals
Atmospheric Pressure
Blue Tilapia
Cell Survival
Cryopreservation - methods
DEHYDRATION
Fish muscle
FREEZING
Histology
Isobaric
Isochoric
MORPHOLOGY
MUSCLES
Muscles - cytology
Muscles - ultrastructure
Osmolar Concentration
PLANT TISSUES
Preservation
THERMODYNAMICS
Tilapia - anatomy & histology
title Isochoric and isobaric freezing of fish muscle
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