Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-13C] pyruvate
Hyperpolarized 13C magnetic resonance spectroscopy (MRS) to assess hepatic metabolism in non-alcoholic fatty liver disease (NAFLD) has not been reported. This study searched for cellular metabolism-based biomarkers for NAFLD induced by a high-fat diet (HFD) in rats. Also, correlations of the biomark...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2017-01, Vol.482 (1), p.112-119 |
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| creator | Moon, Chung-Man Oh, Chang-Hyun Ahn, Kyu-Youn Yang, Jun-Seok Kim, Jae-Young Shin, Sang-Soo Lim, Hyo-Soon Heo, Sook-Hee Seon, Hyun-Ju Kim, Jin-Woong Jeong, Gwang-Woo |
| description | Hyperpolarized 13C magnetic resonance spectroscopy (MRS) to assess hepatic metabolism in non-alcoholic fatty liver disease (NAFLD) has not been reported. This study searched for cellular metabolism-based biomarkers for NAFLD induced by a high-fat diet (HFD) in rats. Also, correlations of the biomarkers with enzyme levels and histopathology were identified during a 6-week follow-up. Six rats were fed a control diet (CD) and seven rats were fed the HFD for 6 weeks. Hyperpolarized 13C dynamic MRS was performed on rat liver following an injection of hyperpolarized [1-13C] pyruvate. Compared with CD-fed rats, HFD-fed rats showed significant increases in the levels of serum alanine aminotransferase and low-density lipoprotein cholesterol at weeks 4 and 6 of follow-up. After the 6-week HFD, the ratios of [1-13C] alanine/pyruvate and [1-13C] lactate/pyruvate were significantly increased, as were the levels of alanine aminotransferase and lactate dehydrogenase, which are potentially associated with hepatosteatosis. The results implicate [1-13C] alanine and [1-13C] lactate as potentially useful noninvasive biomarkers of hepatosteatosis occurring in NAFLD.
[Display omitted]
•Hyperpolarized 13C-alanine and lactate are noninvasive biomarkers on hepatosteatosis.•During the course of HFD feeding, 13C-alanine and lactate were increased in HFD-rats.•Hyperpolarized 13C dynamic MRS will be helpful to monitor the progression of NAFLD. |
| doi_str_mv | 10.1016/j.bbrc.2016.08.118 |
| format | Article |
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[Display omitted]
•Hyperpolarized 13C-alanine and lactate are noninvasive biomarkers on hepatosteatosis.•During the course of HFD feeding, 13C-alanine and lactate were increased in HFD-rats.•Hyperpolarized 13C dynamic MRS will be helpful to monitor the progression of NAFLD.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2016.08.118</identifier><identifier>PMID: 27562716</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Alanine - metabolism ; ALANINES ; Animals ; BIOLOGICAL MARKERS ; Biomarkers - metabolism ; CARBON 13 ; Carbon-13 Magnetic Resonance Spectroscopy - methods ; DIET ; Diet, High-Fat ; Dietary Fats - metabolism ; DISEASES ; Dynamic nuclear polarization (DNP) ; High-fat diet ; Hyperpolarized 13C ; LACTATE DEHYDROGENASE ; Lactic Acid - metabolism ; LIVER ; MAGNETIC RESONANCE ; Male ; METABOLISM ; Non-alcoholic fatty liver disease (NAFLD) ; Non-alcoholic Fatty Liver Disease - diagnosis ; Non-alcoholic Fatty Liver Disease - metabolism ; Pyruvic Acid - pharmacokinetics ; RATS ; Rats, Sprague-Dawley ; Reproducibility of Results ; Sensitivity and Specificity ; SPECTROSCOPY</subject><ispartof>Biochemical and biophysical research communications, 2017-01, Vol.482 (1), p.112-119</ispartof><rights>2016</rights><rights>Copyright © 2016. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-f7d8bf08e382305122efcc67ca87fba551efc810ffd32a648eac28bea373384f3</citedby><cites>FETCH-LOGICAL-c553t-f7d8bf08e382305122efcc67ca87fba551efc810ffd32a648eac28bea373384f3</cites><orcidid>0000-0002-7724-3532</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X16313821$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27562716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22696740$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Moon, Chung-Man</creatorcontrib><creatorcontrib>Oh, Chang-Hyun</creatorcontrib><creatorcontrib>Ahn, Kyu-Youn</creatorcontrib><creatorcontrib>Yang, Jun-Seok</creatorcontrib><creatorcontrib>Kim, Jae-Young</creatorcontrib><creatorcontrib>Shin, Sang-Soo</creatorcontrib><creatorcontrib>Lim, Hyo-Soon</creatorcontrib><creatorcontrib>Heo, Sook-Hee</creatorcontrib><creatorcontrib>Seon, Hyun-Ju</creatorcontrib><creatorcontrib>Kim, Jin-Woong</creatorcontrib><creatorcontrib>Jeong, Gwang-Woo</creatorcontrib><title>Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-13C] pyruvate</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Hyperpolarized 13C magnetic resonance spectroscopy (MRS) to assess hepatic metabolism in non-alcoholic fatty liver disease (NAFLD) has not been reported. This study searched for cellular metabolism-based biomarkers for NAFLD induced by a high-fat diet (HFD) in rats. Also, correlations of the biomarkers with enzyme levels and histopathology were identified during a 6-week follow-up. Six rats were fed a control diet (CD) and seven rats were fed the HFD for 6 weeks. Hyperpolarized 13C dynamic MRS was performed on rat liver following an injection of hyperpolarized [1-13C] pyruvate. Compared with CD-fed rats, HFD-fed rats showed significant increases in the levels of serum alanine aminotransferase and low-density lipoprotein cholesterol at weeks 4 and 6 of follow-up. After the 6-week HFD, the ratios of [1-13C] alanine/pyruvate and [1-13C] lactate/pyruvate were significantly increased, as were the levels of alanine aminotransferase and lactate dehydrogenase, which are potentially associated with hepatosteatosis. The results implicate [1-13C] alanine and [1-13C] lactate as potentially useful noninvasive biomarkers of hepatosteatosis occurring in NAFLD.
[Display omitted]
•Hyperpolarized 13C-alanine and lactate are noninvasive biomarkers on hepatosteatosis.•During the course of HFD feeding, 13C-alanine and lactate were increased in HFD-rats.•Hyperpolarized 13C dynamic MRS will be helpful to monitor the progression of NAFLD.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Alanine - metabolism</subject><subject>ALANINES</subject><subject>Animals</subject><subject>BIOLOGICAL MARKERS</subject><subject>Biomarkers - metabolism</subject><subject>CARBON 13</subject><subject>Carbon-13 Magnetic Resonance Spectroscopy - methods</subject><subject>DIET</subject><subject>Diet, High-Fat</subject><subject>Dietary Fats - metabolism</subject><subject>DISEASES</subject><subject>Dynamic nuclear polarization (DNP)</subject><subject>High-fat diet</subject><subject>Hyperpolarized 13C</subject><subject>LACTATE DEHYDROGENASE</subject><subject>Lactic Acid - metabolism</subject><subject>LIVER</subject><subject>MAGNETIC RESONANCE</subject><subject>Male</subject><subject>METABOLISM</subject><subject>Non-alcoholic fatty liver disease (NAFLD)</subject><subject>Non-alcoholic Fatty Liver Disease - diagnosis</subject><subject>Non-alcoholic Fatty Liver Disease - metabolism</subject><subject>Pyruvic Acid - pharmacokinetics</subject><subject>RATS</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>SPECTROSCOPY</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-q1DAUh4so3vHqC7iQgBs3rUnapqm4kcE_F664URBEQpqe3MnYSWqSFurTzMInmSczda4uxVUSzvc75Jwvyx4TXBBM2PN90XVeFTTdC8wLQvidbENwi3NKcHU322CMWU5b8vkiexDCHmNCKtbezy5oUzPaELbJfr6HKDs3GIU64w7SfwMfkHYeWWdzOSi3-13UMsYFDWYGj3oTQAZAxvaTgv507JbTcWdudnmiUhXiC3RlT8fZzA4d5I2FmDp4CM5KqwCFEVT0Lig3pqDTKbyM4Ec3SG9-QI--kJyU269oXPw0ywgPs3taDgEe3Z6X2ac3rz9u3-XXH95ebV9d56quy5jrpuedxhxKTktcE0pBK8UaJXmjO1nXJL05wVr3JZWs4iAV5R3IsilLXunyMnt67utCNCIoE0HtlLM2_VdQylrWVDhRz87U6N33CUIUBxMUDIO04KYgCGe8pLhu2v9AK9K0jLE6ofSMqrSZ4EGL0ZvkYxEEi1W32ItVt1h1C8xF0p1CT277T90B-r-RP34T8PIMQFrbbMCvU0GS0Bu_DtU786_-vwByNsLG</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Moon, Chung-Man</creator><creator>Oh, Chang-Hyun</creator><creator>Ahn, Kyu-Youn</creator><creator>Yang, Jun-Seok</creator><creator>Kim, Jae-Young</creator><creator>Shin, Sang-Soo</creator><creator>Lim, Hyo-Soon</creator><creator>Heo, Sook-Hee</creator><creator>Seon, Hyun-Ju</creator><creator>Kim, Jin-Woong</creator><creator>Jeong, Gwang-Woo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0002-7724-3532</orcidid></search><sort><creationdate>20170101</creationdate><title>Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-13C] pyruvate</title><author>Moon, Chung-Man ; Oh, Chang-Hyun ; Ahn, Kyu-Youn ; Yang, Jun-Seok ; Kim, Jae-Young ; Shin, Sang-Soo ; Lim, Hyo-Soon ; Heo, Sook-Hee ; Seon, Hyun-Ju ; Kim, Jin-Woong ; Jeong, Gwang-Woo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c553t-f7d8bf08e382305122efcc67ca87fba551efc810ffd32a648eac28bea373384f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Alanine - metabolism</topic><topic>ALANINES</topic><topic>Animals</topic><topic>BIOLOGICAL MARKERS</topic><topic>Biomarkers - metabolism</topic><topic>CARBON 13</topic><topic>Carbon-13 Magnetic Resonance Spectroscopy - methods</topic><topic>DIET</topic><topic>Diet, High-Fat</topic><topic>Dietary Fats - metabolism</topic><topic>DISEASES</topic><topic>Dynamic nuclear polarization (DNP)</topic><topic>High-fat diet</topic><topic>Hyperpolarized 13C</topic><topic>LACTATE DEHYDROGENASE</topic><topic>Lactic Acid - metabolism</topic><topic>LIVER</topic><topic>MAGNETIC RESONANCE</topic><topic>Male</topic><topic>METABOLISM</topic><topic>Non-alcoholic fatty liver disease (NAFLD)</topic><topic>Non-alcoholic Fatty Liver Disease - diagnosis</topic><topic>Non-alcoholic Fatty Liver Disease - metabolism</topic><topic>Pyruvic Acid - pharmacokinetics</topic><topic>RATS</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>SPECTROSCOPY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moon, Chung-Man</creatorcontrib><creatorcontrib>Oh, Chang-Hyun</creatorcontrib><creatorcontrib>Ahn, Kyu-Youn</creatorcontrib><creatorcontrib>Yang, Jun-Seok</creatorcontrib><creatorcontrib>Kim, Jae-Young</creatorcontrib><creatorcontrib>Shin, Sang-Soo</creatorcontrib><creatorcontrib>Lim, Hyo-Soon</creatorcontrib><creatorcontrib>Heo, Sook-Hee</creatorcontrib><creatorcontrib>Seon, Hyun-Ju</creatorcontrib><creatorcontrib>Kim, Jin-Woong</creatorcontrib><creatorcontrib>Jeong, Gwang-Woo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moon, Chung-Man</au><au>Oh, Chang-Hyun</au><au>Ahn, Kyu-Youn</au><au>Yang, Jun-Seok</au><au>Kim, Jae-Young</au><au>Shin, Sang-Soo</au><au>Lim, Hyo-Soon</au><au>Heo, Sook-Hee</au><au>Seon, Hyun-Ju</au><au>Kim, Jin-Woong</au><au>Jeong, Gwang-Woo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-13C] pyruvate</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>482</volume><issue>1</issue><spage>112</spage><epage>119</epage><pages>112-119</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Hyperpolarized 13C magnetic resonance spectroscopy (MRS) to assess hepatic metabolism in non-alcoholic fatty liver disease (NAFLD) has not been reported. This study searched for cellular metabolism-based biomarkers for NAFLD induced by a high-fat diet (HFD) in rats. Also, correlations of the biomarkers with enzyme levels and histopathology were identified during a 6-week follow-up. Six rats were fed a control diet (CD) and seven rats were fed the HFD for 6 weeks. Hyperpolarized 13C dynamic MRS was performed on rat liver following an injection of hyperpolarized [1-13C] pyruvate. Compared with CD-fed rats, HFD-fed rats showed significant increases in the levels of serum alanine aminotransferase and low-density lipoprotein cholesterol at weeks 4 and 6 of follow-up. After the 6-week HFD, the ratios of [1-13C] alanine/pyruvate and [1-13C] lactate/pyruvate were significantly increased, as were the levels of alanine aminotransferase and lactate dehydrogenase, which are potentially associated with hepatosteatosis. The results implicate [1-13C] alanine and [1-13C] lactate as potentially useful noninvasive biomarkers of hepatosteatosis occurring in NAFLD.
[Display omitted]
•Hyperpolarized 13C-alanine and lactate are noninvasive biomarkers on hepatosteatosis.•During the course of HFD feeding, 13C-alanine and lactate were increased in HFD-rats.•Hyperpolarized 13C dynamic MRS will be helpful to monitor the progression of NAFLD.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27562716</pmid><doi>10.1016/j.bbrc.2016.08.118</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7724-3532</orcidid></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2017-01, Vol.482 (1), p.112-119 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 60 APPLIED LIFE SCIENCES Alanine - metabolism ALANINES Animals BIOLOGICAL MARKERS Biomarkers - metabolism CARBON 13 Carbon-13 Magnetic Resonance Spectroscopy - methods DIET Diet, High-Fat Dietary Fats - metabolism DISEASES Dynamic nuclear polarization (DNP) High-fat diet Hyperpolarized 13C LACTATE DEHYDROGENASE Lactic Acid - metabolism LIVER MAGNETIC RESONANCE Male METABOLISM Non-alcoholic fatty liver disease (NAFLD) Non-alcoholic Fatty Liver Disease - diagnosis Non-alcoholic Fatty Liver Disease - metabolism Pyruvic Acid - pharmacokinetics RATS Rats, Sprague-Dawley Reproducibility of Results Sensitivity and Specificity SPECTROSCOPY |
| title | Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-13C] pyruvate |
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