High expression of CREPT promotes tumor growth and is correlated with poor prognosis in colorectal cancer
CREPT (cell cycle-related and expression elevated protein in tumor) is highly expressed in many kinds of cancer, and has been shown to be prognostic in certain cancers. However, the clinical significance of CREPT in colorectal cancer (CRC) has not been sufficiently investigated. In this study, we ex...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2016-11, Vol.480 (3), p.436-442 |
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| creator | Zheng, Guoxu Li, Weimiao Zuo, Baile Guo, Zhangyan Xi, Wenjin Wei, Ming Chen, Peng Wen, Weihong Yang, An-Gang |
| description | CREPT (cell cycle-related and expression elevated protein in tumor) is highly expressed in many kinds of cancer, and has been shown to be prognostic in certain cancers. However, the clinical significance of CREPT in colorectal cancer (CRC) has not been sufficiently investigated. In this study, we examined the CREPT expression in 225 clinical CRC tissues and paired adjacent normal tissues, and analyzed the correlation between CREPT expression and other clinicopathological features. We also evaluated the biological function of CREPT both in vitro and in vivo using knockdown or overexpressing CRC cells. Our results showed that CREPT expressed in 175 of 225 (77.8%) CRC patients and the CREPT expression was significantly associated with tumor differentiation (P = 0.000), Dukes' stages (P = 0.013) and metastasis (P = 0.038). Patients with high CREPT expression tended to have shorter survival time. Multivariate analysis showed that positive CREPT expression can be used as an independent predictor for CRC prognosis. CREPT knockdown cells showed inhibited cell proliferation and arrested cell cycle, while CREPT overexpressing cells showed increased proliferation and promoted cell cycle. In addition, CREPT overexpression significantly promoted tumor growth in vivo. Mechanism study showed that CREPT may regulate cell proliferation and cell cycle through the regulation on cyclin D3, CDK4 and CDK6.
•CREPT expression was associated with decreased overall survival in CRC.•CREPT expression is correlated with tumor progression in CRC.•Overexpression of CREPT promoted tumor growth both in vitro and in vivo. |
| doi_str_mv | 10.1016/j.bbrc.2016.10.067 |
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•CREPT expression was associated with decreased overall survival in CRC.•CREPT expression is correlated with tumor progression in CRC.•Overexpression of CREPT promoted tumor growth both in vitro and in vivo.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2016.10.067</identifier><identifier>PMID: 27773816</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Aged ; Aged, 80 and over ; BIOLOGICAL FUNCTIONS ; Biomarkers, Tumor - metabolism ; Caco-2 Cells ; CELL CYCLE ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; CELL PROLIFERATION ; Cell Survival ; China - epidemiology ; Colorectal cancer ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - mortality ; Colorectal Neoplasms - pathology ; CREPT ; Gene Expression Regulation, Neoplastic ; HT29 Cells ; Humans ; IN VIVO ; Middle Aged ; MULTIVARIATE ANALYSIS ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; NEOPLASMS ; PLANT GROWTH ; Prevalence ; Prognosis ; Reproducibility of Results ; Risk Factors ; Sensitivity and Specificity ; Survival Rate ; SURVIVAL TIME ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2016-11, Vol.480 (3), p.436-442</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-ee015d564f7a41cba0d40082156bbd6d2925766055c38b339aebd82de312acf63</citedby><cites>FETCH-LOGICAL-c524t-ee015d564f7a41cba0d40082156bbd6d2925766055c38b339aebd82de312acf63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X16317454$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27773816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22696699$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Guoxu</creatorcontrib><creatorcontrib>Li, Weimiao</creatorcontrib><creatorcontrib>Zuo, Baile</creatorcontrib><creatorcontrib>Guo, Zhangyan</creatorcontrib><creatorcontrib>Xi, Wenjin</creatorcontrib><creatorcontrib>Wei, Ming</creatorcontrib><creatorcontrib>Chen, Peng</creatorcontrib><creatorcontrib>Wen, Weihong</creatorcontrib><creatorcontrib>Yang, An-Gang</creatorcontrib><title>High expression of CREPT promotes tumor growth and is correlated with poor prognosis in colorectal cancer</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>CREPT (cell cycle-related and expression elevated protein in tumor) is highly expressed in many kinds of cancer, and has been shown to be prognostic in certain cancers. However, the clinical significance of CREPT in colorectal cancer (CRC) has not been sufficiently investigated. In this study, we examined the CREPT expression in 225 clinical CRC tissues and paired adjacent normal tissues, and analyzed the correlation between CREPT expression and other clinicopathological features. We also evaluated the biological function of CREPT both in vitro and in vivo using knockdown or overexpressing CRC cells. Our results showed that CREPT expressed in 175 of 225 (77.8%) CRC patients and the CREPT expression was significantly associated with tumor differentiation (P = 0.000), Dukes' stages (P = 0.013) and metastasis (P = 0.038). Patients with high CREPT expression tended to have shorter survival time. Multivariate analysis showed that positive CREPT expression can be used as an independent predictor for CRC prognosis. CREPT knockdown cells showed inhibited cell proliferation and arrested cell cycle, while CREPT overexpressing cells showed increased proliferation and promoted cell cycle. In addition, CREPT overexpression significantly promoted tumor growth in vivo. Mechanism study showed that CREPT may regulate cell proliferation and cell cycle through the regulation on cyclin D3, CDK4 and CDK6.
•CREPT expression was associated with decreased overall survival in CRC.•CREPT expression is correlated with tumor progression in CRC.•Overexpression of CREPT promoted tumor growth both in vitro and in vivo.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>BIOLOGICAL FUNCTIONS</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Caco-2 Cells</subject><subject>CELL CYCLE</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>CELL PROLIFERATION</subject><subject>Cell Survival</subject><subject>China - epidemiology</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - mortality</subject><subject>Colorectal Neoplasms - pathology</subject><subject>CREPT</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>IN VIVO</subject><subject>Middle Aged</subject><subject>MULTIVARIATE ANALYSIS</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>NEOPLASMS</subject><subject>PLANT GROWTH</subject><subject>Prevalence</subject><subject>Prognosis</subject><subject>Reproducibility of Results</subject><subject>Risk Factors</subject><subject>Sensitivity and Specificity</subject><subject>Survival Rate</subject><subject>SURVIVAL TIME</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVFrFDEUhYNY7Lb6B3yQgC--zPYmM8lMwBdZaisUFKngW8gkd3azzEzWJGvbf2-GrT76lHDudw-Xcwh5y2DNgMmr_brvo13z8i_CGmT7gqwYKKg4g-YlWQGArLhiP8_JRUp7AMYaqV6Rc962bd0xuSL-1m93FB8PEVPyYaZhoJvv19_u6SGGKWRMNB-nEOk2hoe8o2Z21CdqQ4w4moyOPvgiH0JBysZ2DqmM_VyIMUS02YzUmtlifE3OBjMmfPP8XpIfn6_vN7fV3debL5tPd5UVvMkVIjDhhGyG1jTM9gZcA9BxJmTfO-m44qKVEoSwddfXtTLYu447rBk3dpD1JXl_8g0pe52sz2h3NsxzOUZzLpWUShXqw4kqR_86Ysp68sniOJoZwzFp1tVCcKVaXlB-Qm0MKUUc9CH6ycQnzUAvRei9XorQSxGLVoooS--e_Y_9hO7fyt_kC_DxBGDJ4rfHuJyKJSjnl9i0C_5__n8AIjqZ8g</recordid><startdate>20161118</startdate><enddate>20161118</enddate><creator>Zheng, Guoxu</creator><creator>Li, Weimiao</creator><creator>Zuo, Baile</creator><creator>Guo, Zhangyan</creator><creator>Xi, Wenjin</creator><creator>Wei, Ming</creator><creator>Chen, Peng</creator><creator>Wen, Weihong</creator><creator>Yang, An-Gang</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20161118</creationdate><title>High expression of CREPT promotes tumor growth and is correlated with poor prognosis in colorectal cancer</title><author>Zheng, Guoxu ; Li, Weimiao ; Zuo, Baile ; Guo, Zhangyan ; Xi, Wenjin ; Wei, Ming ; Chen, Peng ; Wen, Weihong ; Yang, An-Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-ee015d564f7a41cba0d40082156bbd6d2925766055c38b339aebd82de312acf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>BIOLOGICAL FUNCTIONS</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Caco-2 Cells</topic><topic>CELL CYCLE</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>CELL PROLIFERATION</topic><topic>Cell Survival</topic><topic>China - epidemiology</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - mortality</topic><topic>Colorectal Neoplasms - pathology</topic><topic>CREPT</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>IN VIVO</topic><topic>Middle Aged</topic><topic>MULTIVARIATE ANALYSIS</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>NEOPLASMS</topic><topic>PLANT GROWTH</topic><topic>Prevalence</topic><topic>Prognosis</topic><topic>Reproducibility of Results</topic><topic>Risk Factors</topic><topic>Sensitivity and Specificity</topic><topic>Survival Rate</topic><topic>SURVIVAL TIME</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Guoxu</creatorcontrib><creatorcontrib>Li, Weimiao</creatorcontrib><creatorcontrib>Zuo, Baile</creatorcontrib><creatorcontrib>Guo, Zhangyan</creatorcontrib><creatorcontrib>Xi, Wenjin</creatorcontrib><creatorcontrib>Wei, Ming</creatorcontrib><creatorcontrib>Chen, Peng</creatorcontrib><creatorcontrib>Wen, Weihong</creatorcontrib><creatorcontrib>Yang, An-Gang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Guoxu</au><au>Li, Weimiao</au><au>Zuo, Baile</au><au>Guo, Zhangyan</au><au>Xi, Wenjin</au><au>Wei, Ming</au><au>Chen, Peng</au><au>Wen, Weihong</au><au>Yang, An-Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High expression of CREPT promotes tumor growth and is correlated with poor prognosis in colorectal cancer</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2016-11-18</date><risdate>2016</risdate><volume>480</volume><issue>3</issue><spage>436</spage><epage>442</epage><pages>436-442</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>CREPT (cell cycle-related and expression elevated protein in tumor) is highly expressed in many kinds of cancer, and has been shown to be prognostic in certain cancers. However, the clinical significance of CREPT in colorectal cancer (CRC) has not been sufficiently investigated. In this study, we examined the CREPT expression in 225 clinical CRC tissues and paired adjacent normal tissues, and analyzed the correlation between CREPT expression and other clinicopathological features. We also evaluated the biological function of CREPT both in vitro and in vivo using knockdown or overexpressing CRC cells. Our results showed that CREPT expressed in 175 of 225 (77.8%) CRC patients and the CREPT expression was significantly associated with tumor differentiation (P = 0.000), Dukes' stages (P = 0.013) and metastasis (P = 0.038). Patients with high CREPT expression tended to have shorter survival time. Multivariate analysis showed that positive CREPT expression can be used as an independent predictor for CRC prognosis. CREPT knockdown cells showed inhibited cell proliferation and arrested cell cycle, while CREPT overexpressing cells showed increased proliferation and promoted cell cycle. In addition, CREPT overexpression significantly promoted tumor growth in vivo. Mechanism study showed that CREPT may regulate cell proliferation and cell cycle through the regulation on cyclin D3, CDK4 and CDK6.
•CREPT expression was associated with decreased overall survival in CRC.•CREPT expression is correlated with tumor progression in CRC.•Overexpression of CREPT promoted tumor growth both in vitro and in vivo.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27773816</pmid><doi>10.1016/j.bbrc.2016.10.067</doi><tpages>7</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Aged Aged, 80 and over BIOLOGICAL FUNCTIONS Biomarkers, Tumor - metabolism Caco-2 Cells CELL CYCLE Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism CELL PROLIFERATION Cell Survival China - epidemiology Colorectal cancer Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - mortality Colorectal Neoplasms - pathology CREPT Gene Expression Regulation, Neoplastic HT29 Cells Humans IN VIVO Middle Aged MULTIVARIATE ANALYSIS Neoplasm Proteins - genetics Neoplasm Proteins - metabolism NEOPLASMS PLANT GROWTH Prevalence Prognosis Reproducibility of Results Risk Factors Sensitivity and Specificity Survival Rate SURVIVAL TIME Up-Regulation |
| title | High expression of CREPT promotes tumor growth and is correlated with poor prognosis in colorectal cancer |
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