MacoNPV baculovirus midgut-specific gene expression during infection of the bertha armyworm, Mamestra configurata
Abstract Baculoviruses have two forms, occlusion derived virus (ODV) which is responsible for primary infection in host midgut tissue and budded virus (BV), which infects all other host tissues during secondary infection. This study examined the primary infection by ODV of midgut cells of bertha arm...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2016-12, Vol.499, p.1-8 |
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| creator | Donly, B. Cameron Kaplanoglu, Emine Theilmann, David A Baldwin, Doug Sieminska, Edyta Hegedus, Dwayne D Erlandson, Martin A |
| description | Abstract Baculoviruses have two forms, occlusion derived virus (ODV) which is responsible for primary infection in host midgut tissue and budded virus (BV), which infects all other host tissues during secondary infection. This study examined the primary infection by ODV of midgut cells of bertha armyworm Mamestra configurata fourth instar larvae and measured the expression of viral genes over a time course of infection. Both digital PCR and RNA sequencing methods showed the profile of transcription to be different from those produced by AcMNPV BV infection of in vitro cell cultures. This included having unique collections of genes expressed early, as well as much greater late gene expression of p6.9 and much reduced expression of polh and p10 . These differences likely reflect characteristics unique to the critical step of in vivo midgut cell infection, and provide insights into the processes that regulate viral gene expression in different host tissues. |
| doi_str_mv | 10.1016/j.virol.2016.09.001 |
| format | Article |
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Cameron ; Kaplanoglu, Emine ; Theilmann, David A ; Baldwin, Doug ; Sieminska, Edyta ; Hegedus, Dwayne D ; Erlandson, Martin A</creator><creatorcontrib>Donly, B. Cameron ; Kaplanoglu, Emine ; Theilmann, David A ; Baldwin, Doug ; Sieminska, Edyta ; Hegedus, Dwayne D ; Erlandson, Martin A</creatorcontrib><description>Abstract Baculoviruses have two forms, occlusion derived virus (ODV) which is responsible for primary infection in host midgut tissue and budded virus (BV), which infects all other host tissues during secondary infection. This study examined the primary infection by ODV of midgut cells of bertha armyworm Mamestra configurata fourth instar larvae and measured the expression of viral genes over a time course of infection. Both digital PCR and RNA sequencing methods showed the profile of transcription to be different from those produced by AcMNPV BV infection of in vitro cell cultures. This included having unique collections of genes expressed early, as well as much greater late gene expression of p6.9 and much reduced expression of polh and p10 . These differences likely reflect characteristics unique to the critical step of in vivo midgut cell infection, and provide insights into the processes that regulate viral gene expression in different host tissues.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2016.09.001</identifier><identifier>PMID: 27623563</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ANIMAL TISSUES ; Animals ; Baculoviridae - genetics ; Baculovirus ; CELL CULTURES ; Digital PCR ; Gastrointestinal Tract - virology ; Gene Expression Regulation, Viral ; GENES ; High-Throughput Nucleotide Sequencing ; IN VITRO ; IN VIVO ; Infectious Disease ; Larva - virology ; LARVAE ; MacoNPV ; Mamestra configurata ; Moths - virology ; Occlusion derived virus ; Open Reading Frames ; POLYMERASE CHAIN REACTION ; PROTEINS ; Real-Time Polymerase Chain Reaction ; RNA ; RNA sequencing ; TISSUE CULTURES ; TRANSCRIPTION ; Transcription, Genetic ; Transcriptome ; Transcriptomics ; VIRUSES</subject><ispartof>Virology (New York, N.Y.), 2016-12, Vol.499, p.1-8</ispartof><rights>2016</rights><rights>Crown Copyright © 2016. 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Cameron</creatorcontrib><creatorcontrib>Kaplanoglu, Emine</creatorcontrib><creatorcontrib>Theilmann, David A</creatorcontrib><creatorcontrib>Baldwin, Doug</creatorcontrib><creatorcontrib>Sieminska, Edyta</creatorcontrib><creatorcontrib>Hegedus, Dwayne D</creatorcontrib><creatorcontrib>Erlandson, Martin A</creatorcontrib><title>MacoNPV baculovirus midgut-specific gene expression during infection of the bertha armyworm, Mamestra configurata</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract Baculoviruses have two forms, occlusion derived virus (ODV) which is responsible for primary infection in host midgut tissue and budded virus (BV), which infects all other host tissues during secondary infection. This study examined the primary infection by ODV of midgut cells of bertha armyworm Mamestra configurata fourth instar larvae and measured the expression of viral genes over a time course of infection. Both digital PCR and RNA sequencing methods showed the profile of transcription to be different from those produced by AcMNPV BV infection of in vitro cell cultures. This included having unique collections of genes expressed early, as well as much greater late gene expression of p6.9 and much reduced expression of polh and p10 . These differences likely reflect characteristics unique to the critical step of in vivo midgut cell infection, and provide insights into the processes that regulate viral gene expression in different host tissues.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>CELL CULTURES</subject><subject>Digital PCR</subject><subject>Gastrointestinal Tract - virology</subject><subject>Gene Expression Regulation, Viral</subject><subject>GENES</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>IN VITRO</subject><subject>IN VIVO</subject><subject>Infectious Disease</subject><subject>Larva - virology</subject><subject>LARVAE</subject><subject>MacoNPV</subject><subject>Mamestra configurata</subject><subject>Moths - virology</subject><subject>Occlusion derived virus</subject><subject>Open Reading Frames</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>PROTEINS</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>TISSUE CULTURES</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic</subject><subject>Transcriptome</subject><subject>Transcriptomics</subject><subject>VIRUSES</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi0EotvCL0BClrhwIGFs58sHKqGKAlILSHxcLa8z3vWS2Fs7Key_x2ELBy6crLGed-bVvEPIEwYlA9a83JW3Loah5LkoQZYA7B5ZMZBNAaJi98kKoOJF03F-Qk5T2kGu2xYekhPeNlzUjViRm2ttwodP3-ham3kIueOc6Oj6zTwVaY_GWWfoBj1S_LmPmJILnvZzdH5DnbdopuUjWDptka4xTltNdRwPP0IcX9BrPWKaoqYmeOs2c9STfkQeWD0kfHz3npGvl2--XLwrrj6-fX_x-qowVddOhbEWa5Cil7rrKsbrqua9kFWtwcpGail7q1vWay07UZtW8ApYDS3w3nRYoTgjz459Q5qcSsZNaLbZh8-eFeeN5Jx3mXp-pPYx3MzZrBpdMjgM2mOYk2KdkEwIIUVGxRE1MaQU0ap9dKOOB8VALYmonfqdiFoSUSBVTiSrnt4NmNcj9n81fyLIwKsjgHkZtw7j4hW9wd7FxWof3H8GnP-jN4PzzujhOx4w7cIcfd6zYipxBerzchTLTbBGAK9FK34BrayzOQ</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Donly, B. Cameron</creator><creator>Kaplanoglu, Emine</creator><creator>Theilmann, David A</creator><creator>Baldwin, Doug</creator><creator>Sieminska, Edyta</creator><creator>Hegedus, Dwayne D</creator><creator>Erlandson, Martin A</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20161201</creationdate><title>MacoNPV baculovirus midgut-specific gene expression during infection of the bertha armyworm, Mamestra configurata</title><author>Donly, B. 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Cameron</creatorcontrib><creatorcontrib>Kaplanoglu, Emine</creatorcontrib><creatorcontrib>Theilmann, David A</creatorcontrib><creatorcontrib>Baldwin, Doug</creatorcontrib><creatorcontrib>Sieminska, Edyta</creatorcontrib><creatorcontrib>Hegedus, Dwayne D</creatorcontrib><creatorcontrib>Erlandson, Martin A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Donly, B. 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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 60 APPLIED LIFE SCIENCES ANIMAL TISSUES Animals Baculoviridae - genetics Baculovirus CELL CULTURES Digital PCR Gastrointestinal Tract - virology Gene Expression Regulation, Viral GENES High-Throughput Nucleotide Sequencing IN VITRO IN VIVO Infectious Disease Larva - virology LARVAE MacoNPV Mamestra configurata Moths - virology Occlusion derived virus Open Reading Frames POLYMERASE CHAIN REACTION PROTEINS Real-Time Polymerase Chain Reaction RNA RNA sequencing TISSUE CULTURES TRANSCRIPTION Transcription, Genetic Transcriptome Transcriptomics VIRUSES |
| title | MacoNPV baculovirus midgut-specific gene expression during infection of the bertha armyworm, Mamestra configurata |
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