Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway
This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry re...
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| Veröffentlicht in: | Experimental cell research 2016-07, Vol.345 (2), p.218-229 |
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| creator | Liu, Ru-Ming Sun, Ren-Gang Zhang, Ling-Tao Zhang, Qing-Fang Chen, Dai-Xiong Zhong, Jian-Jiang Xiao, Jian-Hui |
| description | This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry revealed that HA did not change the cytophenotypes of hAMSCs. Additionally, the osteogenic, chondrogenic, and adipogenic differentiation capabilities of these hAMSCs were retained after HA treatment. Moreover, HA increased the mRNA expressions of wnt1, wnt3a, wnt8a, cyclin D1, Ki-67, and β-catenin as well as the protein level of β-catenin and cyclin D1 in hAMSCs; and the nuclear localization of β-catenin was also enhanced. Furthermore, the pro-proliferative effect of HA and up-regulated expression of Wnt/β-catenin pathway-associated proteins - wnt3a, β-catenin and cyclin D1 in hAMSCs were significantly inhibited upon pre-treatment with Wnt-C59, an inhibitor of the Wnt/β-catenin pathway. These results suggest that HA may positively regulate hAMSCs proliferation through regulation of the Wnt/β-catenin signaling pathway.
•Hyaluronic acid (HA) could promote the proliferation of hAMSCs.•HA treatment dose not affect the pluripotency of hAMSCs.•HA increases hAMSCs proliferation through activation of Wnt/β-catenin signaling. |
| doi_str_mv | 10.1016/j.yexcr.2016.05.019 |
| format | Article |
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•Hyaluronic acid (HA) could promote the proliferation of hAMSCs.•HA treatment dose not affect the pluripotency of hAMSCs.•HA increases hAMSCs proliferation through activation of Wnt/β-catenin signaling.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2016.05.019</identifier><identifier>PMID: 27237096</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Amnion - cytology ; Benzeneacetamides - pharmacology ; beta Catenin - metabolism ; Cell Differentiation - drug effects ; Cell Lineage - drug effects ; CELL PROLIFERATION ; Cell Proliferation - drug effects ; Cell Shape - drug effects ; Cyclin D1 - metabolism ; DOSES ; Female ; Fluorescent Antibody Technique ; Gene Expression Regulation - drug effects ; Human amniotic mesenchymal stem cell ; HUMAN POPULATIONS ; Humans ; HYALURONIC ACID ; Hyaluronic Acid - pharmacology ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - metabolism ; MESSENGER-RNA ; Osteogenesis - drug effects ; Phenotype ; PLANT GROWTH ; POTASSIUM IODIDES ; Pregnancy ; Pro-proliferative effect ; Proliferation ; PROTEINS ; Proto-Oncogene Proteins c-myc - metabolism ; Pyridines - pharmacology ; SIGNALS ; STEM CELLS ; TIME DEPENDENCE ; Wnt Signaling Pathway - drug effects ; Wnt Signaling Pathway - genetics ; Wnt/β-catenin signaling pathway</subject><ispartof>Experimental cell research, 2016-07, Vol.345 (2), p.218-229</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-b857c76373235ac547b1cd2d8cb6442d36c79de83b094566bcccc7bf739e10093</citedby><cites>FETCH-LOGICAL-c420t-b857c76373235ac547b1cd2d8cb6442d36c79de83b094566bcccc7bf739e10093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2016.05.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27237096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22648598$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Ru-Ming</creatorcontrib><creatorcontrib>Sun, Ren-Gang</creatorcontrib><creatorcontrib>Zhang, Ling-Tao</creatorcontrib><creatorcontrib>Zhang, Qing-Fang</creatorcontrib><creatorcontrib>Chen, Dai-Xiong</creatorcontrib><creatorcontrib>Zhong, Jian-Jiang</creatorcontrib><creatorcontrib>Xiao, Jian-Hui</creatorcontrib><title>Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry revealed that HA did not change the cytophenotypes of hAMSCs. Additionally, the osteogenic, chondrogenic, and adipogenic differentiation capabilities of these hAMSCs were retained after HA treatment. Moreover, HA increased the mRNA expressions of wnt1, wnt3a, wnt8a, cyclin D1, Ki-67, and β-catenin as well as the protein level of β-catenin and cyclin D1 in hAMSCs; and the nuclear localization of β-catenin was also enhanced. Furthermore, the pro-proliferative effect of HA and up-regulated expression of Wnt/β-catenin pathway-associated proteins - wnt3a, β-catenin and cyclin D1 in hAMSCs were significantly inhibited upon pre-treatment with Wnt-C59, an inhibitor of the Wnt/β-catenin pathway. These results suggest that HA may positively regulate hAMSCs proliferation through regulation of the Wnt/β-catenin signaling pathway.
•Hyaluronic acid (HA) could promote the proliferation of hAMSCs.•HA treatment dose not affect the pluripotency of hAMSCs.•HA increases hAMSCs proliferation through activation of Wnt/β-catenin signaling.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Amnion - cytology</subject><subject>Benzeneacetamides - pharmacology</subject><subject>beta Catenin - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Lineage - drug effects</subject><subject>CELL PROLIFERATION</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Cyclin D1 - metabolism</subject><subject>DOSES</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Human amniotic mesenchymal stem cell</subject><subject>HUMAN POPULATIONS</subject><subject>Humans</subject><subject>HYALURONIC ACID</subject><subject>Hyaluronic Acid - pharmacology</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>MESSENGER-RNA</subject><subject>Osteogenesis - drug effects</subject><subject>Phenotype</subject><subject>PLANT GROWTH</subject><subject>POTASSIUM IODIDES</subject><subject>Pregnancy</subject><subject>Pro-proliferative effect</subject><subject>Proliferation</subject><subject>PROTEINS</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>Pyridines - pharmacology</subject><subject>SIGNALS</subject><subject>STEM CELLS</subject><subject>TIME DEPENDENCE</subject><subject>Wnt Signaling Pathway - drug effects</subject><subject>Wnt Signaling Pathway - genetics</subject><subject>Wnt/β-catenin signaling pathway</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1u1TAUhS0Eoo_CCpCQJSZMkvoniZ0BA1QBRarEBMTQcpybFz8l9sN2CtlAF9SFsCYcXukQ4cmVpe_co3MPQi8pKSmhzcWhXOGnCSXLn5LUJaHtI7SjpCUFqxh7jHaE0KqoJBNn6FmMB0KIlLR5is6YYFyQttmh26tVT0vwzhqsje0xuFE7AxEfg5_sAEEn6x32Ax6XWTusZ2d9yvQMEZwZ11lPOCaYsYFpijiNwS_7MS9L9uZB-82li193hdEJnHU42r3Tk3V7fNRp_KHX5-jJoKcIL-7nOfr64f2Xy6vi-vPHT5fvrgtTMZKKTtbCiIYLznitTV2Jjpqe9dJ0TVWxnjdGtD1I3pG2qpumM_mJbhC8BUpIy8_R69NeH5NV0dgEZjTeOTBJMdZUsm5lpt6cqHyD7wvEpGYbt3jagV-iopJSmQ3q_0EJp5RxxjLKT6gJPsYAgzoGO-uwKkrU1qg6qD-Nqq1RRWqVG82qV_cGSzdD_6D5W2EG3p4AyHe7sRC2WLkZ6G3YUvXe_tPgN3HFtZg</recordid><startdate>20160715</startdate><enddate>20160715</enddate><creator>Liu, Ru-Ming</creator><creator>Sun, Ren-Gang</creator><creator>Zhang, Ling-Tao</creator><creator>Zhang, Qing-Fang</creator><creator>Chen, Dai-Xiong</creator><creator>Zhong, Jian-Jiang</creator><creator>Xiao, Jian-Hui</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope></search><sort><creationdate>20160715</creationdate><title>Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway</title><author>Liu, Ru-Ming ; Sun, Ren-Gang ; Zhang, Ling-Tao ; Zhang, Qing-Fang ; Chen, Dai-Xiong ; Zhong, Jian-Jiang ; Xiao, Jian-Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-b857c76373235ac547b1cd2d8cb6442d36c79de83b094566bcccc7bf739e10093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Amnion - cytology</topic><topic>Benzeneacetamides - pharmacology</topic><topic>beta Catenin - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Lineage - drug effects</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Shape - drug effects</topic><topic>Cyclin D1 - metabolism</topic><topic>DOSES</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Human amniotic mesenchymal stem cell</topic><topic>HUMAN POPULATIONS</topic><topic>Humans</topic><topic>HYALURONIC ACID</topic><topic>Hyaluronic Acid - pharmacology</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>MESSENGER-RNA</topic><topic>Osteogenesis - drug effects</topic><topic>Phenotype</topic><topic>PLANT GROWTH</topic><topic>POTASSIUM IODIDES</topic><topic>Pregnancy</topic><topic>Pro-proliferative effect</topic><topic>Proliferation</topic><topic>PROTEINS</topic><topic>Proto-Oncogene Proteins c-myc - metabolism</topic><topic>Pyridines - pharmacology</topic><topic>SIGNALS</topic><topic>STEM CELLS</topic><topic>TIME DEPENDENCE</topic><topic>Wnt Signaling Pathway - drug effects</topic><topic>Wnt Signaling Pathway - genetics</topic><topic>Wnt/β-catenin signaling pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Ru-Ming</creatorcontrib><creatorcontrib>Sun, Ren-Gang</creatorcontrib><creatorcontrib>Zhang, Ling-Tao</creatorcontrib><creatorcontrib>Zhang, Qing-Fang</creatorcontrib><creatorcontrib>Chen, Dai-Xiong</creatorcontrib><creatorcontrib>Zhong, Jian-Jiang</creatorcontrib><creatorcontrib>Xiao, Jian-Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Ru-Ming</au><au>Sun, Ren-Gang</au><au>Zhang, Ling-Tao</au><au>Zhang, Qing-Fang</au><au>Chen, Dai-Xiong</au><au>Zhong, Jian-Jiang</au><au>Xiao, Jian-Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2016-07-15</date><risdate>2016</risdate><volume>345</volume><issue>2</issue><spage>218</spage><epage>229</epage><pages>218-229</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry revealed that HA did not change the cytophenotypes of hAMSCs. Additionally, the osteogenic, chondrogenic, and adipogenic differentiation capabilities of these hAMSCs were retained after HA treatment. Moreover, HA increased the mRNA expressions of wnt1, wnt3a, wnt8a, cyclin D1, Ki-67, and β-catenin as well as the protein level of β-catenin and cyclin D1 in hAMSCs; and the nuclear localization of β-catenin was also enhanced. Furthermore, the pro-proliferative effect of HA and up-regulated expression of Wnt/β-catenin pathway-associated proteins - wnt3a, β-catenin and cyclin D1 in hAMSCs were significantly inhibited upon pre-treatment with Wnt-C59, an inhibitor of the Wnt/β-catenin pathway. These results suggest that HA may positively regulate hAMSCs proliferation through regulation of the Wnt/β-catenin signaling pathway.
•Hyaluronic acid (HA) could promote the proliferation of hAMSCs.•HA treatment dose not affect the pluripotency of hAMSCs.•HA increases hAMSCs proliferation through activation of Wnt/β-catenin signaling.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27237096</pmid><doi>10.1016/j.yexcr.2016.05.019</doi><tpages>12</tpages></addata></record> |
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| ispartof | Experimental cell research, 2016-07, Vol.345 (2), p.218-229 |
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| subjects | 60 APPLIED LIFE SCIENCES Amnion - cytology Benzeneacetamides - pharmacology beta Catenin - metabolism Cell Differentiation - drug effects Cell Lineage - drug effects CELL PROLIFERATION Cell Proliferation - drug effects Cell Shape - drug effects Cyclin D1 - metabolism DOSES Female Fluorescent Antibody Technique Gene Expression Regulation - drug effects Human amniotic mesenchymal stem cell HUMAN POPULATIONS Humans HYALURONIC ACID Hyaluronic Acid - pharmacology Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - metabolism MESSENGER-RNA Osteogenesis - drug effects Phenotype PLANT GROWTH POTASSIUM IODIDES Pregnancy Pro-proliferative effect Proliferation PROTEINS Proto-Oncogene Proteins c-myc - metabolism Pyridines - pharmacology SIGNALS STEM CELLS TIME DEPENDENCE Wnt Signaling Pathway - drug effects Wnt Signaling Pathway - genetics Wnt/β-catenin signaling pathway |
| title | Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway |
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