Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3
Differentiation of preadipocyte, also called adipogenesis, leads to the phenotype of mature adipocyte. However, excessive adipogenesis is closely linked to the development of obesity. Artesunate, one of artemisinin-type sesquiterpene lactones from Artemisia annua L., is known for anti-malarial and a...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2016-05, Vol.474 (1), p.220-225 |
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| description | Differentiation of preadipocyte, also called adipogenesis, leads to the phenotype of mature adipocyte. However, excessive adipogenesis is closely linked to the development of obesity. Artesunate, one of artemisinin-type sesquiterpene lactones from Artemisia annua L., is known for anti-malarial and anti-cancerous activities. In this study, we investigated the effect of artesunate on adipogenesis in 3T3-L1 preadipocytes. Artesunate strongly inhibited lipid accumulation and triglyceride (TG) synthesis during the differentiation of 3T3-L1 preadipocytes into adipocytes at 5 μM concentration. Artesunate at 5 μM also reduced not only the expressions of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3 (STAT-3) during adipocyte differentiation. Moreover, artesunate at 5 μM reduced leptin, but not adiponectin, mRNA expression during adipocyte differentiation. Taken together, these findings demonstrate that artesunate inhibits adipogenesis in 3T3-L1 preadipoytes through the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
•Artesunate, an artemisinin derivative, inhibits adipogenesis.•Artesunate inhibits C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3 in 3T3-L1 adipocytes.•Artesunate reduces leptin, but not adiponectin, expression in 3T3-L1 adipocytes.•Artesunate thus may have therapeutic potential against obesity. |
| doi_str_mv | 10.1016/j.bbrc.2016.04.109 |
| format | Article |
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•Artesunate, an artemisinin derivative, inhibits adipogenesis.•Artesunate inhibits C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3 in 3T3-L1 adipocytes.•Artesunate reduces leptin, but not adiponectin, expression in 3T3-L1 adipocytes.•Artesunate thus may have therapeutic potential against obesity.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2016.04.109</identifier><identifier>PMID: 27109481</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>3T3 Cells ; 60 APPLIED LIFE SCIENCES ; ADENOSINE ; Adipogenesis ; Adipogenesis - drug effects ; Adipogenesis - physiology ; Animals ; Artemisia annua ; Artemisinins - administration & dosage ; Artesunate ; C/EBP-α ; CARBOXYLIC ACIDS ; CATTLE ; CCAAT-Enhancer-Binding Protein-alpha - metabolism ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; CHEMILUMINESCENCE ; CONCENTRATION RATIO ; FAS ; fas Receptor - metabolism ; LACTONES ; LEPTIN ; MESSENGER-RNA ; METABOLIC DISEASES ; Mice ; Perilipin-1 - metabolism ; PHENOTYPE ; PHOSPHORYLATION ; Phosphorylation - drug effects ; PPAR gamma - metabolism ; PPAR-γ ; RECEPTORS ; STAT-3 ; STAT3 Transcription Factor - metabolism ; TRANSCRIPTION ; TRIGLYCERIDES</subject><ispartof>Biochemical and biophysical research communications, 2016-05, Vol.474 (1), p.220-225</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-159a9abb47c44a7a9c95d7d583077c5e854974d24b28ecf035bc78fe2d33a1de3</citedby><cites>FETCH-LOGICAL-c417t-159a9abb47c44a7a9c95d7d583077c5e854974d24b28ecf035bc78fe2d33a1de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2016.04.109$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27109481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22598739$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Jang, Byeong-Churl</creatorcontrib><title>Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Differentiation of preadipocyte, also called adipogenesis, leads to the phenotype of mature adipocyte. However, excessive adipogenesis is closely linked to the development of obesity. Artesunate, one of artemisinin-type sesquiterpene lactones from Artemisia annua L., is known for anti-malarial and anti-cancerous activities. In this study, we investigated the effect of artesunate on adipogenesis in 3T3-L1 preadipocytes. Artesunate strongly inhibited lipid accumulation and triglyceride (TG) synthesis during the differentiation of 3T3-L1 preadipocytes into adipocytes at 5 μM concentration. Artesunate at 5 μM also reduced not only the expressions of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3 (STAT-3) during adipocyte differentiation. Moreover, artesunate at 5 μM reduced leptin, but not adiponectin, mRNA expression during adipocyte differentiation. Taken together, these findings demonstrate that artesunate inhibits adipogenesis in 3T3-L1 preadipoytes through the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
•Artesunate, an artemisinin derivative, inhibits adipogenesis.•Artesunate inhibits C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3 in 3T3-L1 adipocytes.•Artesunate reduces leptin, but not adiponectin, expression in 3T3-L1 adipocytes.•Artesunate thus may have therapeutic potential against obesity.</description><subject>3T3 Cells</subject><subject>60 APPLIED LIFE SCIENCES</subject><subject>ADENOSINE</subject><subject>Adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>Adipogenesis - physiology</subject><subject>Animals</subject><subject>Artemisia annua</subject><subject>Artemisinins - administration & dosage</subject><subject>Artesunate</subject><subject>C/EBP-α</subject><subject>CARBOXYLIC ACIDS</subject><subject>CATTLE</subject><subject>CCAAT-Enhancer-Binding Protein-alpha - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>CHEMILUMINESCENCE</subject><subject>CONCENTRATION RATIO</subject><subject>FAS</subject><subject>fas Receptor - metabolism</subject><subject>LACTONES</subject><subject>LEPTIN</subject><subject>MESSENGER-RNA</subject><subject>METABOLIC DISEASES</subject><subject>Mice</subject><subject>Perilipin-1 - metabolism</subject><subject>PHENOTYPE</subject><subject>PHOSPHORYLATION</subject><subject>Phosphorylation - drug effects</subject><subject>PPAR gamma - metabolism</subject><subject>PPAR-γ</subject><subject>RECEPTORS</subject><subject>STAT-3</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>TRANSCRIPTION</subject><subject>TRIGLYCERIDES</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhSMEYsrAC7BAltiwaFo7duJYYhOqGUCqRMUUiZ3l2DdTV2mcsZMRfZ55ghHvMc-EQweWsPCPjr977OuTJK8JXhBMiuV-UddeL7K4X2AWNfEkmcUZpxnB7GkywxgXaSbI97PkRQh7jAlhhXienGU8Yqwks-Su8gOEsVMDINvtbG2HgJSxvbuGDmyIIqJbmq4J6j38PtDHWIHqI_JgRm27azTsAMGPeB6CdR1SnVk6j_qdC3H4Y6uGSW7hFtqAXINWy4sPm_Thfo42m-pr-vBzji6rqznqwdvW9vHKaj65oKtttU3py-RZo9oArx7X8-Tb5cV29Sldf_n4eVWtU80IH1KSCyVUXTOuGVNcCS1yw01eUsy5zqHMmeDMZKzOStANpnmtedlAZihVxAA9T96efF0YrAzaDqB32nUd6EFmWS5KTkWk3p2o3rubEcIgDzZoaFvVgRuDJCUui6LkhP8f5SVnnFJcRDQ7odq7EDw0svf2oPxREiyntOVeTmnLKW2JWdSmp7x59B_rA5i_JX_ijcD7ExA_Hm4t-Kkr6DQY66emjLP_8v8FrZC7KA</recordid><startdate>20160520</startdate><enddate>20160520</enddate><creator>Jang, Byeong-Churl</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>OTOTI</scope></search><sort><creationdate>20160520</creationdate><title>Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3</title><author>Jang, Byeong-Churl</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-159a9abb47c44a7a9c95d7d583077c5e854974d24b28ecf035bc78fe2d33a1de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>3T3 Cells</topic><topic>60 APPLIED LIFE SCIENCES</topic><topic>ADENOSINE</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>Adipogenesis - physiology</topic><topic>Animals</topic><topic>Artemisia annua</topic><topic>Artemisinins - administration & dosage</topic><topic>Artesunate</topic><topic>C/EBP-α</topic><topic>CARBOXYLIC ACIDS</topic><topic>CATTLE</topic><topic>CCAAT-Enhancer-Binding Protein-alpha - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>CHEMILUMINESCENCE</topic><topic>CONCENTRATION RATIO</topic><topic>FAS</topic><topic>fas Receptor - metabolism</topic><topic>LACTONES</topic><topic>LEPTIN</topic><topic>MESSENGER-RNA</topic><topic>METABOLIC DISEASES</topic><topic>Mice</topic><topic>Perilipin-1 - metabolism</topic><topic>PHENOTYPE</topic><topic>PHOSPHORYLATION</topic><topic>Phosphorylation - drug effects</topic><topic>PPAR gamma - metabolism</topic><topic>PPAR-γ</topic><topic>RECEPTORS</topic><topic>STAT-3</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>TRANSCRIPTION</topic><topic>TRIGLYCERIDES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jang, Byeong-Churl</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jang, Byeong-Churl</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2016-05-20</date><risdate>2016</risdate><volume>474</volume><issue>1</issue><spage>220</spage><epage>225</epage><pages>220-225</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Differentiation of preadipocyte, also called adipogenesis, leads to the phenotype of mature adipocyte. However, excessive adipogenesis is closely linked to the development of obesity. Artesunate, one of artemisinin-type sesquiterpene lactones from Artemisia annua L., is known for anti-malarial and anti-cancerous activities. In this study, we investigated the effect of artesunate on adipogenesis in 3T3-L1 preadipocytes. Artesunate strongly inhibited lipid accumulation and triglyceride (TG) synthesis during the differentiation of 3T3-L1 preadipocytes into adipocytes at 5 μM concentration. Artesunate at 5 μM also reduced not only the expressions of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3 (STAT-3) during adipocyte differentiation. Moreover, artesunate at 5 μM reduced leptin, but not adiponectin, mRNA expression during adipocyte differentiation. Taken together, these findings demonstrate that artesunate inhibits adipogenesis in 3T3-L1 preadipoytes through the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
•Artesunate, an artemisinin derivative, inhibits adipogenesis.•Artesunate inhibits C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3 in 3T3-L1 adipocytes.•Artesunate reduces leptin, but not adiponectin, expression in 3T3-L1 adipocytes.•Artesunate thus may have therapeutic potential against obesity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27109481</pmid><doi>10.1016/j.bbrc.2016.04.109</doi><tpages>6</tpages></addata></record> |
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| subjects | 3T3 Cells 60 APPLIED LIFE SCIENCES ADENOSINE Adipogenesis Adipogenesis - drug effects Adipogenesis - physiology Animals Artemisia annua Artemisinins - administration & dosage Artesunate C/EBP-α CARBOXYLIC ACIDS CATTLE CCAAT-Enhancer-Binding Protein-alpha - metabolism Cell Differentiation - drug effects Cell Differentiation - physiology CHEMILUMINESCENCE CONCENTRATION RATIO FAS fas Receptor - metabolism LACTONES LEPTIN MESSENGER-RNA METABOLIC DISEASES Mice Perilipin-1 - metabolism PHENOTYPE PHOSPHORYLATION Phosphorylation - drug effects PPAR gamma - metabolism PPAR-γ RECEPTORS STAT-3 STAT3 Transcription Factor - metabolism TRANSCRIPTION TRIGLYCERIDES |
| title | Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3 |
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