LDH-A promotes malignant progression via activation of epithelial-to-mesenchymal transition and conferring stemness in muscle-invasive bladder cancer
Lactate dehydrogenase-A(LDH-A) is an important rate-limiting enzyme in the Warburg effect. Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression. The results of in vitro experiment indicated that LDH-A promotes MIBC cells proliferation, invasion and migration. The pos...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2016-01, Vol.469 (4), p.985-992 |
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| creator | Jiang, Fujin Ma, Song Xue, Yubao Hou, Jianquan Zhang, Yongjie |
| description | Lactate dehydrogenase-A(LDH-A) is an important rate-limiting enzyme in the Warburg effect. Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression. The results of in vitro experiment indicated that LDH-A promotes MIBC cells proliferation, invasion and migration. The positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. Thus, we conclude that LDH-A may be a promising target for MIBC.
•Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression.•IHC analysis of 136 MIBC specimens revealed increased LDH-A is correlated with positive Oct4 and negative E-cadherin.•In vitro experiments demonstrated LDH-A promotes MIBC progression by positive regulation of EMT/CSC. |
| doi_str_mv | 10.1016/j.bbrc.2015.12.078 |
| format | Article |
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•Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression.•IHC analysis of 136 MIBC specimens revealed increased LDH-A is correlated with positive Oct4 and negative E-cadherin.•In vitro experiments demonstrated LDH-A promotes MIBC progression by positive regulation of EMT/CSC.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2015.12.078</identifier><identifier>PMID: 26721441</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Biomarkers, Tumor - metabolism ; BLADDER ; Carcinogenesis - metabolism ; Carcinogenesis - pathology ; Cell Differentiation ; Cell Line, Tumor ; CELL PROLIFERATION ; China - epidemiology ; E-cadherin ; Epithelial-Mesenchymal Transition ; Epithelial-to-mesenchymal transition ; Female ; Histone deacetylases ; HISTONES ; Humans ; IN VITRO ; Isoenzymes - metabolism ; L-Lactate Dehydrogenase - metabolism ; LACTATE DEHYDROGENASE ; Lactate dehydrogenase-A ; LACTATES ; Male ; Middle Aged ; Muscle, Smooth - enzymology ; Muscle, Smooth - pathology ; Muscle-invasive bladder cancer ; MUSCLES ; Neoplasm Invasiveness ; NEOPLASMS ; Neoplastic Stem Cells - enzymology ; Neoplastic Stem Cells - pathology ; Oct4 ; Prevalence ; STEM CELLS ; Survival Analysis ; Urinary Bladder Neoplasms - enzymology ; Urinary Bladder Neoplasms - mortality ; Urinary Bladder Neoplasms - pathology</subject><ispartof>Biochemical and biophysical research communications, 2016-01, Vol.469 (4), p.985-992</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-a69ccc63430ac693a770aa918b41238fc48ce52183814565d17afdf1c03edb9d3</citedby><cites>FETCH-LOGICAL-c520t-a69ccc63430ac693a770aa918b41238fc48ce52183814565d17afdf1c03edb9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2015.12.078$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26721441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22594192$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Fujin</creatorcontrib><creatorcontrib>Ma, Song</creatorcontrib><creatorcontrib>Xue, Yubao</creatorcontrib><creatorcontrib>Hou, Jianquan</creatorcontrib><creatorcontrib>Zhang, Yongjie</creatorcontrib><title>LDH-A promotes malignant progression via activation of epithelial-to-mesenchymal transition and conferring stemness in muscle-invasive bladder cancer</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Lactate dehydrogenase-A(LDH-A) is an important rate-limiting enzyme in the Warburg effect. Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression. The results of in vitro experiment indicated that LDH-A promotes MIBC cells proliferation, invasion and migration. The positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. Thus, we conclude that LDH-A may be a promising target for MIBC.
•Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression.•IHC analysis of 136 MIBC specimens revealed increased LDH-A is correlated with positive Oct4 and negative E-cadherin.•In vitro experiments demonstrated LDH-A promotes MIBC progression by positive regulation of EMT/CSC.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>BLADDER</subject><subject>Carcinogenesis - metabolism</subject><subject>Carcinogenesis - pathology</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>CELL PROLIFERATION</subject><subject>China - epidemiology</subject><subject>E-cadherin</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>Epithelial-to-mesenchymal transition</subject><subject>Female</subject><subject>Histone deacetylases</subject><subject>HISTONES</subject><subject>Humans</subject><subject>IN VITRO</subject><subject>Isoenzymes - metabolism</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>LACTATE DEHYDROGENASE</subject><subject>Lactate dehydrogenase-A</subject><subject>LACTATES</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Muscle, Smooth - enzymology</subject><subject>Muscle, Smooth - pathology</subject><subject>Muscle-invasive bladder cancer</subject><subject>MUSCLES</subject><subject>Neoplasm Invasiveness</subject><subject>NEOPLASMS</subject><subject>Neoplastic Stem Cells - enzymology</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Oct4</subject><subject>Prevalence</subject><subject>STEM CELLS</subject><subject>Survival Analysis</subject><subject>Urinary Bladder Neoplasms - enzymology</subject><subject>Urinary Bladder Neoplasms - mortality</subject><subject>Urinary Bladder Neoplasms - pathology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGLFDEUhIMo7rj6BzxIwIuXHvPS6XQ3eFl21RUGvCh4C-nk9UyG7mRMMg37Q_y_pp3Vo6fwwldFUUXIa2BbYCDfH7fDEM2WM2i2wLes7Z6QDbCeVRyYeEo2jDFZ8R5-XJEXKR0ZAxCyf06uuGw5CAEb8mt3d1_d0FMMc8iY6Kwnt_fa5_VrHzElFzxdnKbaZLfovJ5hpHhy-YCT01OVQzVjQm8OD0VNc9Q-uT-c9paa4EeM0fk9TRlnXxyp83Q-JzNh5fyik1uQDpO2FiM12huML8mzUU8JXz2-1-T7p4_fbu-r3dfPX25vdpVpOMuVlr0xRtaiZtrIvtZty7TuoRsE8LobjegMNhy6ugPRyMZCq0c7gmE12qG39TV5e_ENKTuVjMtoDiWxR5MV500voOeFenehSiU_z5iyml0yOE3aYzgnBa1kBZNSFJRfUBNDShFHdYpu1vFBAVPraOqo1tHUOpoCrspoRfTm0f88zGj_Sf6uVIAPFwBLF4vDuEYthaN1cU1qg_uf_2_dkqtN</recordid><startdate>20160122</startdate><enddate>20160122</enddate><creator>Jiang, Fujin</creator><creator>Ma, Song</creator><creator>Xue, Yubao</creator><creator>Hou, Jianquan</creator><creator>Zhang, Yongjie</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20160122</creationdate><title>LDH-A promotes malignant progression via activation of epithelial-to-mesenchymal transition and conferring stemness in muscle-invasive bladder cancer</title><author>Jiang, Fujin ; Ma, Song ; Xue, Yubao ; Hou, Jianquan ; Zhang, Yongjie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-a69ccc63430ac693a770aa918b41238fc48ce52183814565d17afdf1c03edb9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>BLADDER</topic><topic>Carcinogenesis - metabolism</topic><topic>Carcinogenesis - pathology</topic><topic>Cell Differentiation</topic><topic>Cell Line, Tumor</topic><topic>CELL PROLIFERATION</topic><topic>China - epidemiology</topic><topic>E-cadherin</topic><topic>Epithelial-Mesenchymal Transition</topic><topic>Epithelial-to-mesenchymal transition</topic><topic>Female</topic><topic>Histone deacetylases</topic><topic>HISTONES</topic><topic>Humans</topic><topic>IN VITRO</topic><topic>Isoenzymes - metabolism</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>LACTATE DEHYDROGENASE</topic><topic>Lactate dehydrogenase-A</topic><topic>LACTATES</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Muscle, Smooth - enzymology</topic><topic>Muscle, Smooth - pathology</topic><topic>Muscle-invasive bladder cancer</topic><topic>MUSCLES</topic><topic>Neoplasm Invasiveness</topic><topic>NEOPLASMS</topic><topic>Neoplastic Stem Cells - enzymology</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Oct4</topic><topic>Prevalence</topic><topic>STEM CELLS</topic><topic>Survival Analysis</topic><topic>Urinary Bladder Neoplasms - enzymology</topic><topic>Urinary Bladder Neoplasms - mortality</topic><topic>Urinary Bladder Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Fujin</creatorcontrib><creatorcontrib>Ma, Song</creatorcontrib><creatorcontrib>Xue, Yubao</creatorcontrib><creatorcontrib>Hou, Jianquan</creatorcontrib><creatorcontrib>Zhang, Yongjie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Fujin</au><au>Ma, Song</au><au>Xue, Yubao</au><au>Hou, Jianquan</au><au>Zhang, Yongjie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LDH-A promotes malignant progression via activation of epithelial-to-mesenchymal transition and conferring stemness in muscle-invasive bladder cancer</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2016-01-22</date><risdate>2016</risdate><volume>469</volume><issue>4</issue><spage>985</spage><epage>992</epage><pages>985-992</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Lactate dehydrogenase-A(LDH-A) is an important rate-limiting enzyme in the Warburg effect. Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression. The results of in vitro experiment indicated that LDH-A promotes MIBC cells proliferation, invasion and migration. The positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. Thus, we conclude that LDH-A may be a promising target for MIBC.
•Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression.•IHC analysis of 136 MIBC specimens revealed increased LDH-A is correlated with positive Oct4 and negative E-cadherin.•In vitro experiments demonstrated LDH-A promotes MIBC progression by positive regulation of EMT/CSC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26721441</pmid><doi>10.1016/j.bbrc.2015.12.078</doi><tpages>8</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Biomarkers, Tumor - metabolism BLADDER Carcinogenesis - metabolism Carcinogenesis - pathology Cell Differentiation Cell Line, Tumor CELL PROLIFERATION China - epidemiology E-cadherin Epithelial-Mesenchymal Transition Epithelial-to-mesenchymal transition Female Histone deacetylases HISTONES Humans IN VITRO Isoenzymes - metabolism L-Lactate Dehydrogenase - metabolism LACTATE DEHYDROGENASE Lactate dehydrogenase-A LACTATES Male Middle Aged Muscle, Smooth - enzymology Muscle, Smooth - pathology Muscle-invasive bladder cancer MUSCLES Neoplasm Invasiveness NEOPLASMS Neoplastic Stem Cells - enzymology Neoplastic Stem Cells - pathology Oct4 Prevalence STEM CELLS Survival Analysis Urinary Bladder Neoplasms - enzymology Urinary Bladder Neoplasms - mortality Urinary Bladder Neoplasms - pathology |
| title | LDH-A promotes malignant progression via activation of epithelial-to-mesenchymal transition and conferring stemness in muscle-invasive bladder cancer |
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