TRPM7 is required for ovarian cancer cell growth, migration and invasion
•Silence of TRPM7 in ovarian cancer cells inhibits cell proliferation, migration and invasion.•Silence of TRPM7 decreases phosphorylation levels of Akt, Src and p38 in ovarian cancer cells.•Silence of TRPM7 increases expression of filamentous actin and number of focal adhesions in ovarian cancer cel...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2014-11, Vol.454 (4), p.547-553 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Wang, Jing Liao, Qian-jin Zhang, Yi Zhou, Hui Luo, Chen-hui Tang, Jie Wang, Ying Tang, Yan Zhao, Min Zhao, Xue-heng Zhang, Qiong-yu Xiao, Ling |
| description | •Silence of TRPM7 in ovarian cancer cells inhibits cell proliferation, migration and invasion.•Silence of TRPM7 decreases phosphorylation levels of Akt, Src and p38 in ovarian cancer cells.•Silence of TRPM7 increases expression of filamentous actin and number of focal adhesions in ovarian cancer cells.
Our previous study demonstrated that the melastatin-related transient receptor potential channel 7 (TRPM7) was highly expressed in ovarian carcinomas and its overexpression was significantly associated with poor prognosis in ovarian cancer patients. However, the function of TRPM7 in ovarian cancer is mostly unknown. In this study, we examined the roles of TRPM7 in ovarian cancer cell proliferation, migration and invasion. We found that short hairpin RNA interference-mediated silence of TRPM7 significantly inhibited cell proliferation, colony formation, migration and invasion in multiple ovarian cancer cell lines. Mechanistic investigation revealed that silence of TRPM7 decreased phosphorylation levels of Akt, Src and p38 and increased filamentous actin and focal adhesion number in ovarian cancer cells. Thus, our results suggest that TRPM7 is required for proliferation, migration and invasion of ovarian cancer cells through regulating multiple signaling transduction pathways and the formation of focal adhesions. |
| doi_str_mv | 10.1016/j.bbrc.2014.10.118 |
| format | Article |
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Our previous study demonstrated that the melastatin-related transient receptor potential channel 7 (TRPM7) was highly expressed in ovarian carcinomas and its overexpression was significantly associated with poor prognosis in ovarian cancer patients. However, the function of TRPM7 in ovarian cancer is mostly unknown. In this study, we examined the roles of TRPM7 in ovarian cancer cell proliferation, migration and invasion. We found that short hairpin RNA interference-mediated silence of TRPM7 significantly inhibited cell proliferation, colony formation, migration and invasion in multiple ovarian cancer cell lines. Mechanistic investigation revealed that silence of TRPM7 decreased phosphorylation levels of Akt, Src and p38 and increased filamentous actin and focal adhesion number in ovarian cancer cells. Thus, our results suggest that TRPM7 is required for proliferation, migration and invasion of ovarian cancer cells through regulating multiple signaling transduction pathways and the formation of focal adhesions.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.10.118</identifier><identifier>PMID: 25450691</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; ACTIN ; ADHESION ; CARCINOMAS ; CATIONS ; Cell Line, Tumor ; Cell Movement ; CELL PROLIFERATION ; COLONY FORMATION ; Female ; HEK293 Cells ; Humans ; INTERFERENCE ; Ion channel ; METASTASES ; Metastasis ; MIGRATION ; Ovarian cancer ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; OVARIES ; PATIENTS ; PHOSPHORYLATION ; Protein-Serine-Threonine Kinases - metabolism ; RECEPTORS ; RNA ; Signal Transduction ; TRANSIENTS ; TRPM Cation Channels - metabolism ; TRPM7</subject><ispartof>Biochemical and biophysical research communications, 2014-11, Vol.454 (4), p.547-553</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-576c5dd31a06cf76bb8e474df41055314ecf120735d27dc8c9a76a94e6d989053</citedby><cites>FETCH-LOGICAL-c450t-576c5dd31a06cf76bb8e474df41055314ecf120735d27dc8c9a76a94e6d989053</cites><orcidid>0000-0003-0351-2784</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2014.10.118$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25450691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22416845$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Liao, Qian-jin</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Zhou, Hui</creatorcontrib><creatorcontrib>Luo, Chen-hui</creatorcontrib><creatorcontrib>Tang, Jie</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Tang, Yan</creatorcontrib><creatorcontrib>Zhao, Min</creatorcontrib><creatorcontrib>Zhao, Xue-heng</creatorcontrib><creatorcontrib>Zhang, Qiong-yu</creatorcontrib><creatorcontrib>Xiao, Ling</creatorcontrib><title>TRPM7 is required for ovarian cancer cell growth, migration and invasion</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•Silence of TRPM7 in ovarian cancer cells inhibits cell proliferation, migration and invasion.•Silence of TRPM7 decreases phosphorylation levels of Akt, Src and p38 in ovarian cancer cells.•Silence of TRPM7 increases expression of filamentous actin and number of focal adhesions in ovarian cancer cells.
Our previous study demonstrated that the melastatin-related transient receptor potential channel 7 (TRPM7) was highly expressed in ovarian carcinomas and its overexpression was significantly associated with poor prognosis in ovarian cancer patients. However, the function of TRPM7 in ovarian cancer is mostly unknown. In this study, we examined the roles of TRPM7 in ovarian cancer cell proliferation, migration and invasion. We found that short hairpin RNA interference-mediated silence of TRPM7 significantly inhibited cell proliferation, colony formation, migration and invasion in multiple ovarian cancer cell lines. Mechanistic investigation revealed that silence of TRPM7 decreased phosphorylation levels of Akt, Src and p38 and increased filamentous actin and focal adhesion number in ovarian cancer cells. Thus, our results suggest that TRPM7 is required for proliferation, migration and invasion of ovarian cancer cells through regulating multiple signaling transduction pathways and the formation of focal adhesions.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ACTIN</subject><subject>ADHESION</subject><subject>CARCINOMAS</subject><subject>CATIONS</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>CELL PROLIFERATION</subject><subject>COLONY FORMATION</subject><subject>Female</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>INTERFERENCE</subject><subject>Ion channel</subject><subject>METASTASES</subject><subject>Metastasis</subject><subject>MIGRATION</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>OVARIES</subject><subject>PATIENTS</subject><subject>PHOSPHORYLATION</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>RECEPTORS</subject><subject>RNA</subject><subject>Signal Transduction</subject><subject>TRANSIENTS</subject><subject>TRPM Cation Channels - metabolism</subject><subject>TRPM7</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpaTYff6CHIuilh3gzo5UlG3opIWkKCQkhgdyELI0TLbtWInm39N9H7qY99jRoeOad0cPYJ4Q5AqqT5bzrkpsLQDmfeti8YzOEFiqBIN-zGQCoSrT4sMf2c14CIErVfmR7opY1qBZn7OLu9uZK85B5opdNSOR5HxOPW5uCHbizg6PEHa1W_DHFX-PTMV-Hx2THEAduB8_DsLW5PA7Zh96uMh291QN2f352d3pRXV7_-Hn6_bJyZeVY1Vq52vsFWlCu16rrGpJa-l4i1PUCJbkeBehF7YX2rnGt1cq2kpRvmxbqxQH7ssuNeQwmuzCSe3JxGMiNRgiJqpET9XVHPaf4sqE8mnXI0y_sQHGTDTYAGoTQuqBih7oUc07Um-cU1jb9Nghm8myWZvJsJs9_etiUoc9v-ZtuTf7fyF-xBfi2A6i42AZK06lUZPriuFzqY_hf_itn74xr</recordid><startdate>20141128</startdate><enddate>20141128</enddate><creator>Wang, Jing</creator><creator>Liao, Qian-jin</creator><creator>Zhang, Yi</creator><creator>Zhou, Hui</creator><creator>Luo, Chen-hui</creator><creator>Tang, Jie</creator><creator>Wang, Ying</creator><creator>Tang, Yan</creator><creator>Zhao, Min</creator><creator>Zhao, Xue-heng</creator><creator>Zhang, Qiong-yu</creator><creator>Xiao, Ling</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0003-0351-2784</orcidid></search><sort><creationdate>20141128</creationdate><title>TRPM7 is required for ovarian cancer cell growth, migration and invasion</title><author>Wang, Jing ; Liao, Qian-jin ; Zhang, Yi ; Zhou, Hui ; Luo, Chen-hui ; Tang, Jie ; Wang, Ying ; Tang, Yan ; Zhao, Min ; Zhao, Xue-heng ; Zhang, Qiong-yu ; Xiao, Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-576c5dd31a06cf76bb8e474df41055314ecf120735d27dc8c9a76a94e6d989053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ACTIN</topic><topic>ADHESION</topic><topic>CARCINOMAS</topic><topic>CATIONS</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>CELL PROLIFERATION</topic><topic>COLONY FORMATION</topic><topic>Female</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>INTERFERENCE</topic><topic>Ion channel</topic><topic>METASTASES</topic><topic>Metastasis</topic><topic>MIGRATION</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>OVARIES</topic><topic>PATIENTS</topic><topic>PHOSPHORYLATION</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>RECEPTORS</topic><topic>RNA</topic><topic>Signal Transduction</topic><topic>TRANSIENTS</topic><topic>TRPM Cation Channels - metabolism</topic><topic>TRPM7</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Liao, Qian-jin</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Zhou, Hui</creatorcontrib><creatorcontrib>Luo, Chen-hui</creatorcontrib><creatorcontrib>Tang, Jie</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Tang, Yan</creatorcontrib><creatorcontrib>Zhao, Min</creatorcontrib><creatorcontrib>Zhao, Xue-heng</creatorcontrib><creatorcontrib>Zhang, Qiong-yu</creatorcontrib><creatorcontrib>Xiao, Ling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jing</au><au>Liao, Qian-jin</au><au>Zhang, Yi</au><au>Zhou, Hui</au><au>Luo, Chen-hui</au><au>Tang, Jie</au><au>Wang, Ying</au><au>Tang, Yan</au><au>Zhao, Min</au><au>Zhao, Xue-heng</au><au>Zhang, Qiong-yu</au><au>Xiao, Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRPM7 is required for ovarian cancer cell growth, migration and invasion</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2014-11-28</date><risdate>2014</risdate><volume>454</volume><issue>4</issue><spage>547</spage><epage>553</epage><pages>547-553</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•Silence of TRPM7 in ovarian cancer cells inhibits cell proliferation, migration and invasion.•Silence of TRPM7 decreases phosphorylation levels of Akt, Src and p38 in ovarian cancer cells.•Silence of TRPM7 increases expression of filamentous actin and number of focal adhesions in ovarian cancer cells.
Our previous study demonstrated that the melastatin-related transient receptor potential channel 7 (TRPM7) was highly expressed in ovarian carcinomas and its overexpression was significantly associated with poor prognosis in ovarian cancer patients. However, the function of TRPM7 in ovarian cancer is mostly unknown. In this study, we examined the roles of TRPM7 in ovarian cancer cell proliferation, migration and invasion. We found that short hairpin RNA interference-mediated silence of TRPM7 significantly inhibited cell proliferation, colony formation, migration and invasion in multiple ovarian cancer cell lines. Mechanistic investigation revealed that silence of TRPM7 decreased phosphorylation levels of Akt, Src and p38 and increased filamentous actin and focal adhesion number in ovarian cancer cells. Thus, our results suggest that TRPM7 is required for proliferation, migration and invasion of ovarian cancer cells through regulating multiple signaling transduction pathways and the formation of focal adhesions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25450691</pmid><doi>10.1016/j.bbrc.2014.10.118</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0351-2784</orcidid></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2014-11, Vol.454 (4), p.547-553 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 60 APPLIED LIFE SCIENCES ACTIN ADHESION CARCINOMAS CATIONS Cell Line, Tumor Cell Movement CELL PROLIFERATION COLONY FORMATION Female HEK293 Cells Humans INTERFERENCE Ion channel METASTASES Metastasis MIGRATION Ovarian cancer Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology OVARIES PATIENTS PHOSPHORYLATION Protein-Serine-Threonine Kinases - metabolism RECEPTORS RNA Signal Transduction TRANSIENTS TRPM Cation Channels - metabolism TRPM7 |
| title | TRPM7 is required for ovarian cancer cell growth, migration and invasion |
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