Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo
•Gene and cell-based therapies comprise innovative aspects of regenerative medicine.•Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS.•Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords.•Over-expressing oct4 and sox2 also...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2014-09, Vol.451 (4), p.503-509 |
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| creator | Guseva, Daria Rizvanov, Albert A. Salafutdinov, Ilnur I. Kudryashova, Nezhdana V. Palotás, András Islamov, Rustem R. |
| description | •Gene and cell-based therapies comprise innovative aspects of regenerative medicine.•Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS.•Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords.•Over-expressing oct4 and sox2 also induced production of neural marker PGP9.5.•Formation of new nerve cells, secreting trophic factors and neo-vascularisation could improve symptoms in ALS.
Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis. |
| doi_str_mv | 10.1016/j.bbrc.2014.07.132 |
| format | Article |
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Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.07.132</identifier><identifier>PMID: 25124662</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Amyotrophic lateral sclerosis ; Amyotrophic Lateral Sclerosis - genetics ; Animals ; BLOOD CELLS ; Cell Dedifferentiation ; Cell Differentiation ; Differentiation ; Disease Models, Animal ; DRUGS ; Fetal Blood - cytology ; GENES ; HUMAN POPULATIONS ; Humans ; IN VIVO ; MICE ; MUSCLES ; NERVE CELLS ; Octamer Transcription Factor-3 - biosynthesis ; Pluripotency ; Pluripotent Stem Cells - metabolism ; Regenerative Medicine ; SOXB1 Transcription Factors - biosynthesis ; SPINAL CORD ; STEM CELLS ; SYMPTOMS ; THERAPY ; Transcription factor ; TRANSCRIPTION FACTORS ; Transfection ; TRANSPLANTS ; Umbilical cord blood mono-nuclear cell</subject><ispartof>Biochemical and biophysical research communications, 2014-09, Vol.451 (4), p.503-509</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-642ea7b365b53a3ee2e32b237954676d2860d6272f160d50f1239eac6add43ed3</citedby><cites>FETCH-LOGICAL-c454t-642ea7b365b53a3ee2e32b237954676d2860d6272f160d50f1239eac6add43ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2014.07.132$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25124662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22416727$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Guseva, Daria</creatorcontrib><creatorcontrib>Rizvanov, Albert A.</creatorcontrib><creatorcontrib>Salafutdinov, Ilnur I.</creatorcontrib><creatorcontrib>Kudryashova, Nezhdana V.</creatorcontrib><creatorcontrib>Palotás, András</creatorcontrib><creatorcontrib>Islamov, Rustem R.</creatorcontrib><title>Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•Gene and cell-based therapies comprise innovative aspects of regenerative medicine.•Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS.•Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords.•Over-expressing oct4 and sox2 also induced production of neural marker PGP9.5.•Formation of new nerve cells, secreting trophic factors and neo-vascularisation could improve symptoms in ALS.
Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Amyotrophic lateral sclerosis</subject><subject>Amyotrophic Lateral Sclerosis - genetics</subject><subject>Animals</subject><subject>BLOOD CELLS</subject><subject>Cell Dedifferentiation</subject><subject>Cell Differentiation</subject><subject>Differentiation</subject><subject>Disease Models, Animal</subject><subject>DRUGS</subject><subject>Fetal Blood - cytology</subject><subject>GENES</subject><subject>HUMAN POPULATIONS</subject><subject>Humans</subject><subject>IN VIVO</subject><subject>MICE</subject><subject>MUSCLES</subject><subject>NERVE CELLS</subject><subject>Octamer Transcription Factor-3 - biosynthesis</subject><subject>Pluripotency</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Regenerative Medicine</subject><subject>SOXB1 Transcription Factors - biosynthesis</subject><subject>SPINAL CORD</subject><subject>STEM CELLS</subject><subject>SYMPTOMS</subject><subject>THERAPY</subject><subject>Transcription factor</subject><subject>TRANSCRIPTION FACTORS</subject><subject>Transfection</subject><subject>TRANSPLANTS</subject><subject>Umbilical cord blood mono-nuclear cell</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFr3DAQhUVpabZJ_kAPRdBLLnalkSzX0EsJSVMI7KEt9CZkacxqsaWtJC8p_fO12W2POc3AfO_xmEfIW85qzrj6sK_7PtkaGJc1a2su4AXZcNaxCjiTL8mGMaYq6PjPC_Im5z1jnEvVvSYX0HCQSsGG_NkeMVX4dEiYs4-BxoFubZHUBEe_xSegJZmQbfKHsp4HY0tMmWLYmWAxU-eHAROG4k0563fzZAKdp96P3pqR2pgc7ccYHbU4jpn6QI_-GK_Iq8GMGa_P85L8uL_7fvtQPW6_fL39_FhZ2chSKQlo2l6opm-EEYiAAnoQbddI1SoHHxVzCloY-LI0bOAgOjRWGeekQCcuyfuTb8zF62x9QbuzMQS0RQNIrlpoF-rmRB1S_DVjLnryec1rAsY5a94okILLrltQOKE2xZwTDvqQ_GTSb82ZXqvRe71Wo9dqNGv1Us0ienf2n_sJ3X_Jvy4W4NMJwOUXR49pjYrLk51Pa1IX_XP-fwF14KAZ</recordid><startdate>20140905</startdate><enddate>20140905</enddate><creator>Guseva, Daria</creator><creator>Rizvanov, Albert A.</creator><creator>Salafutdinov, Ilnur I.</creator><creator>Kudryashova, Nezhdana V.</creator><creator>Palotás, András</creator><creator>Islamov, Rustem R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20140905</creationdate><title>Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo</title><author>Guseva, Daria ; Rizvanov, Albert A. ; Salafutdinov, Ilnur I. ; Kudryashova, Nezhdana V. ; Palotás, András ; Islamov, Rustem R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-642ea7b365b53a3ee2e32b237954676d2860d6272f160d50f1239eac6add43ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Amyotrophic lateral sclerosis</topic><topic>Amyotrophic Lateral Sclerosis - genetics</topic><topic>Animals</topic><topic>BLOOD CELLS</topic><topic>Cell Dedifferentiation</topic><topic>Cell Differentiation</topic><topic>Differentiation</topic><topic>Disease Models, Animal</topic><topic>DRUGS</topic><topic>Fetal Blood - cytology</topic><topic>GENES</topic><topic>HUMAN POPULATIONS</topic><topic>Humans</topic><topic>IN VIVO</topic><topic>MICE</topic><topic>MUSCLES</topic><topic>NERVE CELLS</topic><topic>Octamer Transcription Factor-3 - biosynthesis</topic><topic>Pluripotency</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>Regenerative Medicine</topic><topic>SOXB1 Transcription Factors - biosynthesis</topic><topic>SPINAL CORD</topic><topic>STEM CELLS</topic><topic>SYMPTOMS</topic><topic>THERAPY</topic><topic>Transcription factor</topic><topic>TRANSCRIPTION FACTORS</topic><topic>Transfection</topic><topic>TRANSPLANTS</topic><topic>Umbilical cord blood mono-nuclear cell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guseva, Daria</creatorcontrib><creatorcontrib>Rizvanov, Albert A.</creatorcontrib><creatorcontrib>Salafutdinov, Ilnur I.</creatorcontrib><creatorcontrib>Kudryashova, Nezhdana V.</creatorcontrib><creatorcontrib>Palotás, András</creatorcontrib><creatorcontrib>Islamov, Rustem R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guseva, Daria</au><au>Rizvanov, Albert A.</au><au>Salafutdinov, Ilnur I.</au><au>Kudryashova, Nezhdana V.</au><au>Palotás, András</au><au>Islamov, Rustem R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2014-09-05</date><risdate>2014</risdate><volume>451</volume><issue>4</issue><spage>503</spage><epage>509</epage><pages>503-509</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•Gene and cell-based therapies comprise innovative aspects of regenerative medicine.•Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS.•Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords.•Over-expressing oct4 and sox2 also induced production of neural marker PGP9.5.•Formation of new nerve cells, secreting trophic factors and neo-vascularisation could improve symptoms in ALS.
Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25124662</pmid><doi>10.1016/j.bbrc.2014.07.132</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2014-09, Vol.451 (4), p.503-509 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 60 APPLIED LIFE SCIENCES Amyotrophic lateral sclerosis Amyotrophic Lateral Sclerosis - genetics Animals BLOOD CELLS Cell Dedifferentiation Cell Differentiation Differentiation Disease Models, Animal DRUGS Fetal Blood - cytology GENES HUMAN POPULATIONS Humans IN VIVO MICE MUSCLES NERVE CELLS Octamer Transcription Factor-3 - biosynthesis Pluripotency Pluripotent Stem Cells - metabolism Regenerative Medicine SOXB1 Transcription Factors - biosynthesis SPINAL CORD STEM CELLS SYMPTOMS THERAPY Transcription factor TRANSCRIPTION FACTORS Transfection TRANSPLANTS Umbilical cord blood mono-nuclear cell |
| title | Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo |
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