The histone deacetylase SIRT6 suppresses the expression of the RNA-binding protein PCBP2 in glioma
•PCBP2 expression is over-expressed in human glioma tissues and cell lines.•SIRT6 is decreased in glioma and correlated with PCBP2.•SIRT6 inhibits PCBP2 expression by deacetylating H3K9.•SIRT6 inhibits glioma growth in vitro and in vivo. More than 80% of tumors that occur in the brain are malignant...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2014-03, Vol.446 (1), p.364-369 |
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| creator | Chen, Xin Hao, Bin Liu, Ying Dai, Dongwei Han, Guosheng Li, Yanan Wu, Xi Zhou, Xiaoping Yue, Zhijian Wang, Laixing Cao, Yiqun Liu, Jianmin |
| description | •PCBP2 expression is over-expressed in human glioma tissues and cell lines.•SIRT6 is decreased in glioma and correlated with PCBP2.•SIRT6 inhibits PCBP2 expression by deacetylating H3K9.•SIRT6 inhibits glioma growth in vitro and in vivo.
More than 80% of tumors that occur in the brain are malignant gliomas. The prognosis of glioma patients is still poor, which makes glioma an urgent subject of cancer research. Previous evidence and our present data show that PCBP2 is over-expressed in human glioma tissues and predicts poor outcome. However, the mechanism by which PCBP2 is regulated in glioma remains elusive. We find that SIRT6, one of the NAD+-dependent class III deacetylase SIRTUINs, is down-regulated in human glioma tissues and that the level of SIRT6 is negatively correlated with PCBP2 level while H3K9ac enrichment on the promoter of PCBP2 is positively correlated with PCBP2 expression. Furthermore, we identify PCBP2 as a target of SIRT6. We demonstrate that PCBP2 expression is inhibited by SIRT6, which depends upon deacetylating H3K9ac. Finally, our results reveal that SIRT6 inhibits glioma cell proliferation and colony formation in vitro and glioma cell growth in vivo in a PCBP2 dependent manner. In summary, our findings implicate that SIRT6 inhibits PCBP2 expression through deacetylating H3K9ac and SIRT6 acts as a tumor suppressor in human glioma. |
| doi_str_mv | 10.1016/j.bbrc.2014.02.116 |
| format | Article |
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More than 80% of tumors that occur in the brain are malignant gliomas. The prognosis of glioma patients is still poor, which makes glioma an urgent subject of cancer research. Previous evidence and our present data show that PCBP2 is over-expressed in human glioma tissues and predicts poor outcome. However, the mechanism by which PCBP2 is regulated in glioma remains elusive. We find that SIRT6, one of the NAD+-dependent class III deacetylase SIRTUINs, is down-regulated in human glioma tissues and that the level of SIRT6 is negatively correlated with PCBP2 level while H3K9ac enrichment on the promoter of PCBP2 is positively correlated with PCBP2 expression. Furthermore, we identify PCBP2 as a target of SIRT6. We demonstrate that PCBP2 expression is inhibited by SIRT6, which depends upon deacetylating H3K9ac. Finally, our results reveal that SIRT6 inhibits glioma cell proliferation and colony formation in vitro and glioma cell growth in vivo in a PCBP2 dependent manner. In summary, our findings implicate that SIRT6 inhibits PCBP2 expression through deacetylating H3K9ac and SIRT6 acts as a tumor suppressor in human glioma.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.02.116</identifier><identifier>PMID: 24607900</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Acetylation ; ANIMAL TISSUES ; Animals ; BRAIN ; Brain Neoplasms - genetics ; Brain Neoplasms - metabolism ; Brain Neoplasms - pathology ; Cell Line, Tumor ; CELL PROLIFERATION ; COLONY FORMATION ; Down-Regulation ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Glioma ; Glioma - genetics ; Glioma - metabolism ; Glioma - pathology ; GLIOMAS ; H3K9ac ; Heterografts ; Histones - chemistry ; Histones - metabolism ; HUMAN POPULATIONS ; Humans ; IN VITRO ; IN VIVO ; Mice ; NAD ; PATIENTS ; PCBP2 ; Promoter Regions, Genetic ; PROMOTERS ; RNA ; RNA-Binding Proteins - antagonists & inhibitors ; RNA-Binding Proteins - genetics ; SIRT6 ; Sirtuins - antagonists & inhibitors ; Sirtuins - genetics ; Sirtuins - metabolism ; Tumor Stem Cell Assay ; Tumor Suppressor Proteins - metabolism ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2014-03, Vol.446 (1), p.364-369</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-bae586e4287100f6255fb9bb728c3e9dcc4fe5a278ce3e5c13dc6d7a8f58c23d3</citedby><cites>FETCH-LOGICAL-c417t-bae586e4287100f6255fb9bb728c3e9dcc4fe5a278ce3e5c13dc6d7a8f58c23d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2014.02.116$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,778,782,883,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24607900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22416349$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Hao, Bin</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Dai, Dongwei</creatorcontrib><creatorcontrib>Han, Guosheng</creatorcontrib><creatorcontrib>Li, Yanan</creatorcontrib><creatorcontrib>Wu, Xi</creatorcontrib><creatorcontrib>Zhou, Xiaoping</creatorcontrib><creatorcontrib>Yue, Zhijian</creatorcontrib><creatorcontrib>Wang, Laixing</creatorcontrib><creatorcontrib>Cao, Yiqun</creatorcontrib><creatorcontrib>Liu, Jianmin</creatorcontrib><title>The histone deacetylase SIRT6 suppresses the expression of the RNA-binding protein PCBP2 in glioma</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•PCBP2 expression is over-expressed in human glioma tissues and cell lines.•SIRT6 is decreased in glioma and correlated with PCBP2.•SIRT6 inhibits PCBP2 expression by deacetylating H3K9.•SIRT6 inhibits glioma growth in vitro and in vivo.
More than 80% of tumors that occur in the brain are malignant gliomas. The prognosis of glioma patients is still poor, which makes glioma an urgent subject of cancer research. Previous evidence and our present data show that PCBP2 is over-expressed in human glioma tissues and predicts poor outcome. However, the mechanism by which PCBP2 is regulated in glioma remains elusive. We find that SIRT6, one of the NAD+-dependent class III deacetylase SIRTUINs, is down-regulated in human glioma tissues and that the level of SIRT6 is negatively correlated with PCBP2 level while H3K9ac enrichment on the promoter of PCBP2 is positively correlated with PCBP2 expression. Furthermore, we identify PCBP2 as a target of SIRT6. We demonstrate that PCBP2 expression is inhibited by SIRT6, which depends upon deacetylating H3K9ac. Finally, our results reveal that SIRT6 inhibits glioma cell proliferation and colony formation in vitro and glioma cell growth in vivo in a PCBP2 dependent manner. In summary, our findings implicate that SIRT6 inhibits PCBP2 expression through deacetylating H3K9ac and SIRT6 acts as a tumor suppressor in human glioma.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Acetylation</subject><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>BRAIN</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - metabolism</subject><subject>Brain Neoplasms - pathology</subject><subject>Cell Line, Tumor</subject><subject>CELL PROLIFERATION</subject><subject>COLONY FORMATION</subject><subject>Down-Regulation</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Knockdown Techniques</subject><subject>Glioma</subject><subject>Glioma - genetics</subject><subject>Glioma - metabolism</subject><subject>Glioma - pathology</subject><subject>GLIOMAS</subject><subject>H3K9ac</subject><subject>Heterografts</subject><subject>Histones - chemistry</subject><subject>Histones - metabolism</subject><subject>HUMAN POPULATIONS</subject><subject>Humans</subject><subject>IN VITRO</subject><subject>IN VIVO</subject><subject>Mice</subject><subject>NAD</subject><subject>PATIENTS</subject><subject>PCBP2</subject><subject>Promoter Regions, Genetic</subject><subject>PROMOTERS</subject><subject>RNA</subject><subject>RNA-Binding Proteins - antagonists & inhibitors</subject><subject>RNA-Binding Proteins - genetics</subject><subject>SIRT6</subject><subject>Sirtuins - antagonists & inhibitors</subject><subject>Sirtuins - genetics</subject><subject>Sirtuins - metabolism</subject><subject>Tumor Stem Cell Assay</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERZfCH-CALHHhknTGcZxE4lJWfFSq2qosEjfLsSddr7Lx1s4i-u9JdgtHOM2Hnnk1My9jbxByBFTnm7xto80FoMxB5IjqGVsgNJAJBPmcLQBAZaLBH6fsZUobAESpmhfsVEgFVQOwYO1qTXzt0xgG4o6MpfGxN4n4t8u7leJpv9tFSokSHyeQfh0qHwYeukPn7voia_3g_HDPdzGM5Ad-u_x4K_iU3Pc-bM0rdtKZPtHrp3jGvn_-tFp-za5uvlwuL64yK7Eas9ZQWSuSoq4QoFOiLLu2adtK1LagxlkrOyqNqGpLBZUWC2eVq0zdlbUVhSvO2Lujbkij18n6kezahmEgO2ohJKpCNhP1_khN2z7sKY1665OlvjcDhX3SqKRQWImJ_i9aopSFglpMqDiiNoaUInV6F_3WxEeNoGez9EbPZunZLA1C40H_7ZP-vt2S-zvyx50J-HAEaHrbT09xvooGS87H-SgX_L_0fwOt66Q3</recordid><startdate>20140328</startdate><enddate>20140328</enddate><creator>Chen, Xin</creator><creator>Hao, Bin</creator><creator>Liu, Ying</creator><creator>Dai, Dongwei</creator><creator>Han, Guosheng</creator><creator>Li, Yanan</creator><creator>Wu, Xi</creator><creator>Zhou, Xiaoping</creator><creator>Yue, Zhijian</creator><creator>Wang, Laixing</creator><creator>Cao, Yiqun</creator><creator>Liu, Jianmin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>7TM</scope><scope>OTOTI</scope></search><sort><creationdate>20140328</creationdate><title>The histone deacetylase SIRT6 suppresses the expression of the RNA-binding protein PCBP2 in glioma</title><author>Chen, Xin ; Hao, Bin ; Liu, Ying ; Dai, Dongwei ; Han, Guosheng ; Li, Yanan ; Wu, Xi ; Zhou, Xiaoping ; Yue, Zhijian ; Wang, Laixing ; Cao, Yiqun ; Liu, Jianmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-bae586e4287100f6255fb9bb728c3e9dcc4fe5a278ce3e5c13dc6d7a8f58c23d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Acetylation</topic><topic>ANIMAL TISSUES</topic><topic>Animals</topic><topic>BRAIN</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - metabolism</topic><topic>Brain Neoplasms - pathology</topic><topic>Cell Line, Tumor</topic><topic>CELL PROLIFERATION</topic><topic>COLONY FORMATION</topic><topic>Down-Regulation</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Knockdown Techniques</topic><topic>Glioma</topic><topic>Glioma - genetics</topic><topic>Glioma - metabolism</topic><topic>Glioma - pathology</topic><topic>GLIOMAS</topic><topic>H3K9ac</topic><topic>Heterografts</topic><topic>Histones - chemistry</topic><topic>Histones - metabolism</topic><topic>HUMAN POPULATIONS</topic><topic>Humans</topic><topic>IN VITRO</topic><topic>IN VIVO</topic><topic>Mice</topic><topic>NAD</topic><topic>PATIENTS</topic><topic>PCBP2</topic><topic>Promoter Regions, Genetic</topic><topic>PROMOTERS</topic><topic>RNA</topic><topic>RNA-Binding Proteins - antagonists & inhibitors</topic><topic>RNA-Binding Proteins - genetics</topic><topic>SIRT6</topic><topic>Sirtuins - antagonists & inhibitors</topic><topic>Sirtuins - genetics</topic><topic>Sirtuins - metabolism</topic><topic>Tumor Stem Cell Assay</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Hao, Bin</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Dai, Dongwei</creatorcontrib><creatorcontrib>Han, Guosheng</creatorcontrib><creatorcontrib>Li, Yanan</creatorcontrib><creatorcontrib>Wu, Xi</creatorcontrib><creatorcontrib>Zhou, Xiaoping</creatorcontrib><creatorcontrib>Yue, Zhijian</creatorcontrib><creatorcontrib>Wang, Laixing</creatorcontrib><creatorcontrib>Cao, Yiqun</creatorcontrib><creatorcontrib>Liu, Jianmin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xin</au><au>Hao, Bin</au><au>Liu, Ying</au><au>Dai, Dongwei</au><au>Han, Guosheng</au><au>Li, Yanan</au><au>Wu, Xi</au><au>Zhou, Xiaoping</au><au>Yue, Zhijian</au><au>Wang, Laixing</au><au>Cao, Yiqun</au><au>Liu, Jianmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The histone deacetylase SIRT6 suppresses the expression of the RNA-binding protein PCBP2 in glioma</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2014-03-28</date><risdate>2014</risdate><volume>446</volume><issue>1</issue><spage>364</spage><epage>369</epage><pages>364-369</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•PCBP2 expression is over-expressed in human glioma tissues and cell lines.•SIRT6 is decreased in glioma and correlated with PCBP2.•SIRT6 inhibits PCBP2 expression by deacetylating H3K9.•SIRT6 inhibits glioma growth in vitro and in vivo.
More than 80% of tumors that occur in the brain are malignant gliomas. The prognosis of glioma patients is still poor, which makes glioma an urgent subject of cancer research. Previous evidence and our present data show that PCBP2 is over-expressed in human glioma tissues and predicts poor outcome. However, the mechanism by which PCBP2 is regulated in glioma remains elusive. We find that SIRT6, one of the NAD+-dependent class III deacetylase SIRTUINs, is down-regulated in human glioma tissues and that the level of SIRT6 is negatively correlated with PCBP2 level while H3K9ac enrichment on the promoter of PCBP2 is positively correlated with PCBP2 expression. Furthermore, we identify PCBP2 as a target of SIRT6. We demonstrate that PCBP2 expression is inhibited by SIRT6, which depends upon deacetylating H3K9ac. Finally, our results reveal that SIRT6 inhibits glioma cell proliferation and colony formation in vitro and glioma cell growth in vivo in a PCBP2 dependent manner. In summary, our findings implicate that SIRT6 inhibits PCBP2 expression through deacetylating H3K9ac and SIRT6 acts as a tumor suppressor in human glioma.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24607900</pmid><doi>10.1016/j.bbrc.2014.02.116</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2014-03, Vol.446 (1), p.364-369 |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | 60 APPLIED LIFE SCIENCES Acetylation ANIMAL TISSUES Animals BRAIN Brain Neoplasms - genetics Brain Neoplasms - metabolism Brain Neoplasms - pathology Cell Line, Tumor CELL PROLIFERATION COLONY FORMATION Down-Regulation Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Glioma Glioma - genetics Glioma - metabolism Glioma - pathology GLIOMAS H3K9ac Heterografts Histones - chemistry Histones - metabolism HUMAN POPULATIONS Humans IN VITRO IN VIVO Mice NAD PATIENTS PCBP2 Promoter Regions, Genetic PROMOTERS RNA RNA-Binding Proteins - antagonists & inhibitors RNA-Binding Proteins - genetics SIRT6 Sirtuins - antagonists & inhibitors Sirtuins - genetics Sirtuins - metabolism Tumor Stem Cell Assay Tumor Suppressor Proteins - metabolism Up-Regulation |
| title | The histone deacetylase SIRT6 suppresses the expression of the RNA-binding protein PCBP2 in glioma |
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