A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes

Identifying virulence-critical genes from pathogens is often limited by functional redundancy. To rapidly interrogate the contributions of combinations of genes to a biological outcome, we have developed a mu ltiplex, r andomized C RISPR i nterference s equencing (MuRCiS) approach. At its center is...

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Veröffentlicht in:	eLife 2023-12, Vol.12
Hauptverfasser:	Ellis, Nicole A, Myers, Kevin S, Tung, Jessica, Davidson Ward, Anne, Johnston, Kathryn, Bonnington, Katherine E, Donohue, Timothy J, Machner, Matthias P
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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