Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein

•MyoD was engineered to contain protein transduction domain and endosome-disruptive INF7 peptide.•The engineered MyoD-IT showed efficient nuclear targeting through an endosomal escape by INF7 peptide.•By applying MyoD-IT, human adipose-derived stem cells (hASCs) were differentiated into myogenic cel...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 2013-07, Vol.437 (1), p.156-161
Hauptverfasser:	Sung, Min Sun, Mun, Ji-Young, Kwon, Ohsuk, Kwon, Ki-Sun, Oh, Doo-Byoung
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES Adipose Tissue - cytology Amino Acid Sequence Animals Cell Differentiation Cell Nucleus - metabolism Coculture Techniques Endosomal escape Human adipose-derived stem cells Humans INFLUENZA Intracellular Space - metabolism MICE Molecular Sequence Data Muscle Development Muscle Fibers, Skeletal - metabolism MYOBLASTS Myoblasts - cytology Myoblasts - metabolism MyoD MyoD Protein - metabolism Myogenic differentiation MYOSIN PEPTIDES Peptides - chemistry Peptides - metabolism PH VALUE Protein Engineering Protein transduction domain Protein Transport Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Solubility STEM CELLS Stem Cells - cytology Stem Cells - metabolism TRANSCRIPTION FACTORS Transduction, Genetic
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!