Enrichment and terminal differentiation of striated muscle progenitors in vitro
Enrichment and terminal differentiation of mammalian striated muscle cells is severely hampered by fibroblast overgrowth, de-differentiation and/or lack of functional differentiation. Herein we report a new, reproducible and simple method to enrich and terminally differentiate muscle stem cells and...
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| Veröffentlicht in: | Experimental cell research 2009-10, Vol.315 (16), p.2741-2751 |
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| creator | Becher, Ulrich M. Breitbach, Martin Sasse, Philipp Garbe, Stephan van der Ven, Peter F.M. Fürst, Dieter O. Fleischmann, Bernd K. |
| description | Enrichment and terminal differentiation of mammalian striated muscle cells is severely hampered by fibroblast overgrowth, de-differentiation and/or lack of functional differentiation. Herein we report a new, reproducible and simple method to enrich and terminally differentiate muscle stem cells and progenitors from mice and humans. We show that a single gamma irradiation of muscle cells induces their massive differentiation into structurally and functionally intact myotubes and cardiomyocytes and that these cells can be kept in culture for many weeks. Similar results are also obtained when treating skeletal muscle-derived stem cells and progenitors with Mitomycin C. |
| doi_str_mv | 10.1016/j.yexcr.2009.07.005 |
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Herein we report a new, reproducible and simple method to enrich and terminally differentiate muscle stem cells and progenitors from mice and humans. We show that a single gamma irradiation of muscle cells induces their massive differentiation into structurally and functionally intact myotubes and cardiomyocytes and that these cells can be kept in culture for many weeks. 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| subjects | Animals BIOLOGICAL RADIATION EFFECTS Cell culture Cell Culture Techniques Cell Differentiation - drug effects Cell Differentiation - physiology Cell Differentiation - radiation effects Cells, Cultured Cellular biology FIBROBLASTS GAMMA RADIATION Gamma Rays Humans IN VITRO IRRADIATION Male MICE MITOMYCIN Mitomycin - pharmacology Muscle satellite cells Muscle, Striated - cytology Muscle, Striated - drug effects Muscle, Striated - physiology Muscle, Striated - radiation effects MUSCLES Muscular system Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Nucleic Acid Synthesis Inhibitors - pharmacology Proliferation RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS Scientific method STEM CELLS Stem Cells - cytology Stem Cells - drug effects Stem Cells - physiology Stem Cells - radiation effects Terminal differentiation |
| title | Enrichment and terminal differentiation of striated muscle progenitors in vitro |
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