Endocrine disrupting chemicals affect the adipogenic differentiation of mesenchymal stem cells in distinct ontogenetic windows
► Endocrine disrupting chemicals affect adipogenesis in mesenchymal stem cells (MSC). ► The adipogenic impact depends strongly on the window of exposure. ► Bisphenol A reduces the potential of MSC to differentiate into adipocytes. ► DEHP and TBT trigger the adipogenic differentiation of mesenchymal...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2012-01, Vol.417 (2), p.747-752 |
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| creator | Biemann, Ronald Navarrete Santos, Anne Navarrete Santos, Alexander Riemann, Dagmar Knelangen, Julia Blüher, Matthias Koch, Holger Fischer, Bernd |
| description | ► Endocrine disrupting chemicals affect adipogenesis in mesenchymal stem cells (MSC). ► The adipogenic impact depends strongly on the window of exposure. ► Bisphenol A reduces the potential of MSC to differentiate into adipocytes. ► DEHP and TBT trigger the adipogenic differentiation of mesenchymal stem cells. ► BPA, DEHP and TBT did not affect adipogenesis in embryonic stem cells.
Endocrine disrupting chemicals (EDC) like bisphenol A (BPA), bis(2-ethylhexyl)phthalate (DEHP) and tributyltin (TBT) are ubiquitously present in the environment and in human tissues. They bind to nuclear hormone receptors and affect cellular and developmental processes. In this study, we show that BPA, DEHP and TBT affect the adipogenic differentiation of murine mesenchymal stem cells (MSC, C3H/10T1/2) in a concentration-, stage- and compound-specific manner. C3H/10T1/2 cells and embryonic stem cells (CGR8) were exposed to BPA, DEHP or TBT at different stages of cell determination and differentiation (undifferentiated growth, adipogenic induction and terminal adipogenic differentiation). The final amount of differentiated adipocytes, cellular triglyceride content and mRNA expression of adipogenic marker genes (adiponectin, FABP4, PPARγ2, LPL) were quantified and compared with corresponding unexposed cells. BPA (10μM) decreased subsequent adipogenic differentiation of MSC, when cells were exposed during undifferentiated growth. In contrast, DEHP (100μM) during the hormonal induction period, and TBT (100nM) in all investigated stages, enhanced adipogenesis. Importantly, exposure of undifferentiated murine embryonic stem cells did not show any effect of the investigated EDC on subsequent adipogenic differentiation. |
| doi_str_mv | 10.1016/j.bbrc.2011.12.028 |
| format | Article |
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Endocrine disrupting chemicals (EDC) like bisphenol A (BPA), bis(2-ethylhexyl)phthalate (DEHP) and tributyltin (TBT) are ubiquitously present in the environment and in human tissues. They bind to nuclear hormone receptors and affect cellular and developmental processes. In this study, we show that BPA, DEHP and TBT affect the adipogenic differentiation of murine mesenchymal stem cells (MSC, C3H/10T1/2) in a concentration-, stage- and compound-specific manner. C3H/10T1/2 cells and embryonic stem cells (CGR8) were exposed to BPA, DEHP or TBT at different stages of cell determination and differentiation (undifferentiated growth, adipogenic induction and terminal adipogenic differentiation). The final amount of differentiated adipocytes, cellular triglyceride content and mRNA expression of adipogenic marker genes (adiponectin, FABP4, PPARγ2, LPL) were quantified and compared with corresponding unexposed cells. BPA (10μM) decreased subsequent adipogenic differentiation of MSC, when cells were exposed during undifferentiated growth. In contrast, DEHP (100μM) during the hormonal induction period, and TBT (100nM) in all investigated stages, enhanced adipogenesis. Importantly, exposure of undifferentiated murine embryonic stem cells did not show any effect of the investigated EDC on subsequent adipogenic differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2011.12.028</identifier><identifier>PMID: 22197818</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Adipogenesis ; Adipogenesis - drug effects ; ANIMAL TISSUES ; Animals ; Benzhydryl Compounds ; BPA ; Cell Line ; DEHP ; Diethylhexyl Phthalate - pharmacology ; EDC ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - drug effects ; Endocrine Disruptors - pharmacology ; GENES ; HORMONES ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; MESSENGER-RNA ; Mice ; Phenols - pharmacology ; PHTHALATES ; RECEPTORS ; STEM CELLS ; TBT ; Trialkyltin Compounds - pharmacology ; TRIGLYCERIDES</subject><ispartof>Biochemical and biophysical research communications, 2012-01, Vol.417 (2), p.747-752</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-773f5bf30267f6573c6ab32ac3dd2f5ba7bcc3975e4979dfdbca46ba9e26b92d3</citedby><cites>FETCH-LOGICAL-c383t-773f5bf30267f6573c6ab32ac3dd2f5ba7bcc3975e4979dfdbca46ba9e26b92d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X11022376$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22197818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22207652$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Biemann, Ronald</creatorcontrib><creatorcontrib>Navarrete Santos, Anne</creatorcontrib><creatorcontrib>Navarrete Santos, Alexander</creatorcontrib><creatorcontrib>Riemann, Dagmar</creatorcontrib><creatorcontrib>Knelangen, Julia</creatorcontrib><creatorcontrib>Blüher, Matthias</creatorcontrib><creatorcontrib>Koch, Holger</creatorcontrib><creatorcontrib>Fischer, Bernd</creatorcontrib><title>Endocrine disrupting chemicals affect the adipogenic differentiation of mesenchymal stem cells in distinct ontogenetic windows</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► Endocrine disrupting chemicals affect adipogenesis in mesenchymal stem cells (MSC). ► The adipogenic impact depends strongly on the window of exposure. ► Bisphenol A reduces the potential of MSC to differentiate into adipocytes. ► DEHP and TBT trigger the adipogenic differentiation of mesenchymal stem cells. ► BPA, DEHP and TBT did not affect adipogenesis in embryonic stem cells.
Endocrine disrupting chemicals (EDC) like bisphenol A (BPA), bis(2-ethylhexyl)phthalate (DEHP) and tributyltin (TBT) are ubiquitously present in the environment and in human tissues. They bind to nuclear hormone receptors and affect cellular and developmental processes. In this study, we show that BPA, DEHP and TBT affect the adipogenic differentiation of murine mesenchymal stem cells (MSC, C3H/10T1/2) in a concentration-, stage- and compound-specific manner. C3H/10T1/2 cells and embryonic stem cells (CGR8) were exposed to BPA, DEHP or TBT at different stages of cell determination and differentiation (undifferentiated growth, adipogenic induction and terminal adipogenic differentiation). The final amount of differentiated adipocytes, cellular triglyceride content and mRNA expression of adipogenic marker genes (adiponectin, FABP4, PPARγ2, LPL) were quantified and compared with corresponding unexposed cells. BPA (10μM) decreased subsequent adipogenic differentiation of MSC, when cells were exposed during undifferentiated growth. In contrast, DEHP (100μM) during the hormonal induction period, and TBT (100nM) in all investigated stages, enhanced adipogenesis. Importantly, exposure of undifferentiated murine embryonic stem cells did not show any effect of the investigated EDC on subsequent adipogenic differentiation.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>Benzhydryl Compounds</subject><subject>BPA</subject><subject>Cell Line</subject><subject>DEHP</subject><subject>Diethylhexyl Phthalate - pharmacology</subject><subject>EDC</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - drug effects</subject><subject>Endocrine Disruptors - pharmacology</subject><subject>GENES</subject><subject>HORMONES</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>MESSENGER-RNA</subject><subject>Mice</subject><subject>Phenols - pharmacology</subject><subject>PHTHALATES</subject><subject>RECEPTORS</subject><subject>STEM CELLS</subject><subject>TBT</subject><subject>Trialkyltin Compounds - pharmacology</subject><subject>TRIGLYCERIDES</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuLFDEUhYMoTjv6B1xIgQtXVeZRnVTAjQzjAwbcKLgLedyaTtOVtEl6htn4271Fjy5dBW6-c-7jEPKa0YFRJt_vB-eKHzhlbGB8oHx6QjaMatpzRsenZEMplT3X7OcFeVHrniI4Sv2cXHDOtJrYtCG_r1PIvsQEXYi1nI4tptvO72CJ3h5qZ-cZfOvaDjob4jHfQooeUSwXSC3aFnPq8twtUCH53cNiD11tsHQeDqiPafVFUzTJqa16aOhwH7HvfX1Jns3YBl49vpfkx6fr71df-ptvn79efbzpvZhE65US89bNgnKpZrlVwkvrBLdehMDxxyrnvdBqC6NWOszBeTtKZzVw6TQP4pK8PftmnMVUHxv4nc8p4XKGc06V3HKk3p2pY8m_TlCbWWJd97AJ8qkazZBS46SQ5GfSl1xrgdkcS1xseTCMmjUcszdrOGYNxzBuMBwUvXm0P7kFwj_J3zQQ-HAGAE9xF6Gsk-JVIcSyDhpy_J__H5ANo8k</recordid><startdate>20120113</startdate><enddate>20120113</enddate><creator>Biemann, Ronald</creator><creator>Navarrete Santos, Anne</creator><creator>Navarrete Santos, Alexander</creator><creator>Riemann, Dagmar</creator><creator>Knelangen, Julia</creator><creator>Blüher, Matthias</creator><creator>Koch, Holger</creator><creator>Fischer, Bernd</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20120113</creationdate><title>Endocrine disrupting chemicals affect the adipogenic differentiation of mesenchymal stem cells in distinct ontogenetic windows</title><author>Biemann, Ronald ; Navarrete Santos, Anne ; Navarrete Santos, Alexander ; Riemann, Dagmar ; Knelangen, Julia ; Blüher, Matthias ; Koch, Holger ; Fischer, Bernd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-773f5bf30267f6573c6ab32ac3dd2f5ba7bcc3975e4979dfdbca46ba9e26b92d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>ANIMAL TISSUES</topic><topic>Animals</topic><topic>Benzhydryl Compounds</topic><topic>BPA</topic><topic>Cell Line</topic><topic>DEHP</topic><topic>Diethylhexyl Phthalate - pharmacology</topic><topic>EDC</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - drug effects</topic><topic>Endocrine Disruptors - pharmacology</topic><topic>GENES</topic><topic>HORMONES</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>MESSENGER-RNA</topic><topic>Mice</topic><topic>Phenols - pharmacology</topic><topic>PHTHALATES</topic><topic>RECEPTORS</topic><topic>STEM CELLS</topic><topic>TBT</topic><topic>Trialkyltin Compounds - pharmacology</topic><topic>TRIGLYCERIDES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biemann, Ronald</creatorcontrib><creatorcontrib>Navarrete Santos, Anne</creatorcontrib><creatorcontrib>Navarrete Santos, Alexander</creatorcontrib><creatorcontrib>Riemann, Dagmar</creatorcontrib><creatorcontrib>Knelangen, Julia</creatorcontrib><creatorcontrib>Blüher, Matthias</creatorcontrib><creatorcontrib>Koch, Holger</creatorcontrib><creatorcontrib>Fischer, Bernd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biemann, Ronald</au><au>Navarrete Santos, Anne</au><au>Navarrete Santos, Alexander</au><au>Riemann, Dagmar</au><au>Knelangen, Julia</au><au>Blüher, Matthias</au><au>Koch, Holger</au><au>Fischer, Bernd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endocrine disrupting chemicals affect the adipogenic differentiation of mesenchymal stem cells in distinct ontogenetic windows</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2012-01-13</date><risdate>2012</risdate><volume>417</volume><issue>2</issue><spage>747</spage><epage>752</epage><pages>747-752</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► Endocrine disrupting chemicals affect adipogenesis in mesenchymal stem cells (MSC). ► The adipogenic impact depends strongly on the window of exposure. ► Bisphenol A reduces the potential of MSC to differentiate into adipocytes. ► DEHP and TBT trigger the adipogenic differentiation of mesenchymal stem cells. ► BPA, DEHP and TBT did not affect adipogenesis in embryonic stem cells.
Endocrine disrupting chemicals (EDC) like bisphenol A (BPA), bis(2-ethylhexyl)phthalate (DEHP) and tributyltin (TBT) are ubiquitously present in the environment and in human tissues. They bind to nuclear hormone receptors and affect cellular and developmental processes. In this study, we show that BPA, DEHP and TBT affect the adipogenic differentiation of murine mesenchymal stem cells (MSC, C3H/10T1/2) in a concentration-, stage- and compound-specific manner. C3H/10T1/2 cells and embryonic stem cells (CGR8) were exposed to BPA, DEHP or TBT at different stages of cell determination and differentiation (undifferentiated growth, adipogenic induction and terminal adipogenic differentiation). The final amount of differentiated adipocytes, cellular triglyceride content and mRNA expression of adipogenic marker genes (adiponectin, FABP4, PPARγ2, LPL) were quantified and compared with corresponding unexposed cells. BPA (10μM) decreased subsequent adipogenic differentiation of MSC, when cells were exposed during undifferentiated growth. In contrast, DEHP (100μM) during the hormonal induction period, and TBT (100nM) in all investigated stages, enhanced adipogenesis. Importantly, exposure of undifferentiated murine embryonic stem cells did not show any effect of the investigated EDC on subsequent adipogenic differentiation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22197818</pmid><doi>10.1016/j.bbrc.2011.12.028</doi><tpages>6</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Adipogenesis Adipogenesis - drug effects ANIMAL TISSUES Animals Benzhydryl Compounds BPA Cell Line DEHP Diethylhexyl Phthalate - pharmacology EDC Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Endocrine Disruptors - pharmacology GENES HORMONES Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects MESSENGER-RNA Mice Phenols - pharmacology PHTHALATES RECEPTORS STEM CELLS TBT Trialkyltin Compounds - pharmacology TRIGLYCERIDES |
| title | Endocrine disrupting chemicals affect the adipogenic differentiation of mesenchymal stem cells in distinct ontogenetic windows |
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