Structure and dimerization of translation initiation factor aIF5B in solution

Highlights: Black-Right-Pointing-Pointer aIF5B forms maximum 5.0-6.8% irreversible dimers in solution. Black-Right-Pointing-Pointer Sedimentation coefficients for monomer and dimer are 3.64 and 5.51 {+-} 0.29 S. Black-Right-Pointing-Pointer Adding only 2% glycerol prevents dimerization. Black-Right-...

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Veröffentlicht in:	Biochemical and biophysical research communications 2011-12, Vol.416 (1-2)
Hauptverfasser:	Rasmussen, Louise Caroe Vohlander, Oliveira, Cristiano Luis Pinto, Byron, Olwyn, Jensen, Janni Mosgaard, Pedersen, Jan Skov, Sperling-Petersen, Hans Uffe, Mortensen, Kim Kusk
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES BIOSYNTHESIS CRYSTALLOGRAPHY DIMERIZATION DIMERS ESCHERICHIA COLI GLYCEROL LIGHT SCATTERING MONOMERS PROTEINS SEDIMENTATION SMALL ANGLE SCATTERING SULFUR 29 ULTRACENTRIFUGATION X-RAY DIFFRACTION
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