Effect of heparin and alendronate coating on titanium surfaces on inhibition of osteoclast and enhancement of osteoblast function
► We examine bone metabolism of engineered alendronate attached to Ti surfaces. ► Alendronate-immobilized Ti enhances activation of osteoblast differentiation. ► Alendronate-immobilized Ti inhibits osteoclast differentiation. ► Alendronate-immobilized Ti may be a bioactive implant with dual function...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2011-09, Vol.413 (2), p.194-200 |
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| creator | Moon, Ho-Jin Yun, Young-Pil Han, Choong-Wan Kim, Min Sung Kim, Sung Eun Bae, Min Soo Kim, Gyu-Tae Choi, Yong-Suk Hwang, Eui-Hwan Lee, Joon Woo Lee, Jin-Moo Lee, Chang-Hoon Kim, Duck-Su Kwon, Il Keun |
| description | ► We examine bone metabolism of engineered alendronate attached to Ti surfaces. ► Alendronate-immobilized Ti enhances activation of osteoblast differentiation. ► Alendronate-immobilized Ti inhibits osteoclast differentiation. ► Alendronate-immobilized Ti may be a bioactive implant with dual functions.
The failure of orthopedic and dental implants has been attributed mainly to loosening of the implant from host bone, which may be due to weak bonding of the implant material to bone tissue. Titanium (Ti) is used in the field of orthopedic and dental implants because of its excellent biocompatibility and outstanding mechanical properties. Therefore, in the field of materials science and tissue engineering, there has been extensive research to immobilize bioactive molecules on the surface of implant materials in order to provide the implants with improved adhesion to the host bone tissue.
In this study, chemically active functional groups were introduced on the surface of Ti by a grafting reaction with heparin and then the Ti was functionalized by immobilizing alendronate onto the heparin-grafted surface. In the MC3T3-E1 cell osteogenic differentiation study, the alendronate-immobilized Ti substrates significantly enhanced alkaline phosphatase activity (ALP) and calcium content. Additionally, nuclear factor kappa B ligand (RANKL)-induced osteoclast differentiation of RAW264.7 cells was inhibited with the alendronate-immobilized Ti as confirmed by TRAP analysis. Real time PCR analysis showed that mRNA expressions of osteocalcin and osteopontin, which are markers for osteogenesis, were upregulated in MC3T3-E1 cells cultured on alendronate-immobilized Ti. The mRNA expressions of TRAP and Cathepsin K, markers for osteoclastogenesis, in RAW264.7 cells cultured on alendronate-immobilized Ti were down-regulated. Our study suggests that alendronate-immobilized Ti may be a bioactive implant with dual functions to enhance osteoblast differentiation and to inhibit osteoclast differentiation simultaneously. |
| doi_str_mv | 10.1016/j.bbrc.2011.08.057 |
| format | Article |
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The failure of orthopedic and dental implants has been attributed mainly to loosening of the implant from host bone, which may be due to weak bonding of the implant material to bone tissue. Titanium (Ti) is used in the field of orthopedic and dental implants because of its excellent biocompatibility and outstanding mechanical properties. Therefore, in the field of materials science and tissue engineering, there has been extensive research to immobilize bioactive molecules on the surface of implant materials in order to provide the implants with improved adhesion to the host bone tissue.
In this study, chemically active functional groups were introduced on the surface of Ti by a grafting reaction with heparin and then the Ti was functionalized by immobilizing alendronate onto the heparin-grafted surface. In the MC3T3-E1 cell osteogenic differentiation study, the alendronate-immobilized Ti substrates significantly enhanced alkaline phosphatase activity (ALP) and calcium content. Additionally, nuclear factor kappa B ligand (RANKL)-induced osteoclast differentiation of RAW264.7 cells was inhibited with the alendronate-immobilized Ti as confirmed by TRAP analysis. Real time PCR analysis showed that mRNA expressions of osteocalcin and osteopontin, which are markers for osteogenesis, were upregulated in MC3T3-E1 cells cultured on alendronate-immobilized Ti. The mRNA expressions of TRAP and Cathepsin K, markers for osteoclastogenesis, in RAW264.7 cells cultured on alendronate-immobilized Ti were down-regulated. Our study suggests that alendronate-immobilized Ti may be a bioactive implant with dual functions to enhance osteoblast differentiation and to inhibit osteoclast differentiation simultaneously.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2011.08.057</identifier><identifier>PMID: 21888898</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Acid phosphatase (tartrate-resistant) ; Alendronate ; Alendronate - chemistry ; Alendronate - pharmacology ; Alendronic acid ; ALKALINE PHOSPHATASE ; Animals ; Biocompatibility ; Bisphosphonates ; BONDING ; Bone implants ; BONE TISSUES ; Cathepsin K ; CATHEPSINS ; CELL CULTURES ; Cell Line ; Coated Materials, Biocompatible - chemistry ; Coated Materials, Biocompatible - pharmacology ; Coatings ; CONNECTIVE TISSUE CELLS ; Dental restorative materials ; Gene expression ; GRAFTS ; HEPARIN ; Heparin - chemistry ; Heparin - pharmacology ; INHIBITION ; LIGANDS ; MECHANICAL PROPERTIES ; MESSENGER-RNA ; METABOLISM ; Mice ; Orthopedics ; Osteoblast ; Osteoblastogenesis ; Osteoblasts - drug effects ; Osteocalcin ; Osteoclast ; Osteoclastogenesis ; Osteoclasts ; Osteoclasts - drug effects ; Osteogenesis ; POLYMERASE CHAIN REACTION ; RANK Ligand - pharmacology ; SKELETON ; Surface Properties ; TITANIUM ; Titanium - chemistry</subject><ispartof>Biochemical and biophysical research communications, 2011-09, Vol.413 (2), p.194-200</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-81848afe36ca323b375cba849ca57a05839dc7fc0f0164181cb160500b7888803</citedby><cites>FETCH-LOGICAL-c482t-81848afe36ca323b375cba849ca57a05839dc7fc0f0164181cb160500b7888803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X11014574$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21888898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22207501$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Moon, Ho-Jin</creatorcontrib><creatorcontrib>Yun, Young-Pil</creatorcontrib><creatorcontrib>Han, Choong-Wan</creatorcontrib><creatorcontrib>Kim, Min Sung</creatorcontrib><creatorcontrib>Kim, Sung Eun</creatorcontrib><creatorcontrib>Bae, Min Soo</creatorcontrib><creatorcontrib>Kim, Gyu-Tae</creatorcontrib><creatorcontrib>Choi, Yong-Suk</creatorcontrib><creatorcontrib>Hwang, Eui-Hwan</creatorcontrib><creatorcontrib>Lee, Joon Woo</creatorcontrib><creatorcontrib>Lee, Jin-Moo</creatorcontrib><creatorcontrib>Lee, Chang-Hoon</creatorcontrib><creatorcontrib>Kim, Duck-Su</creatorcontrib><creatorcontrib>Kwon, Il Keun</creatorcontrib><title>Effect of heparin and alendronate coating on titanium surfaces on inhibition of osteoclast and enhancement of osteoblast function</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► We examine bone metabolism of engineered alendronate attached to Ti surfaces. ► Alendronate-immobilized Ti enhances activation of osteoblast differentiation. ► Alendronate-immobilized Ti inhibits osteoclast differentiation. ► Alendronate-immobilized Ti may be a bioactive implant with dual functions.
The failure of orthopedic and dental implants has been attributed mainly to loosening of the implant from host bone, which may be due to weak bonding of the implant material to bone tissue. Titanium (Ti) is used in the field of orthopedic and dental implants because of its excellent biocompatibility and outstanding mechanical properties. Therefore, in the field of materials science and tissue engineering, there has been extensive research to immobilize bioactive molecules on the surface of implant materials in order to provide the implants with improved adhesion to the host bone tissue.
In this study, chemically active functional groups were introduced on the surface of Ti by a grafting reaction with heparin and then the Ti was functionalized by immobilizing alendronate onto the heparin-grafted surface. In the MC3T3-E1 cell osteogenic differentiation study, the alendronate-immobilized Ti substrates significantly enhanced alkaline phosphatase activity (ALP) and calcium content. Additionally, nuclear factor kappa B ligand (RANKL)-induced osteoclast differentiation of RAW264.7 cells was inhibited with the alendronate-immobilized Ti as confirmed by TRAP analysis. Real time PCR analysis showed that mRNA expressions of osteocalcin and osteopontin, which are markers for osteogenesis, were upregulated in MC3T3-E1 cells cultured on alendronate-immobilized Ti. The mRNA expressions of TRAP and Cathepsin K, markers for osteoclastogenesis, in RAW264.7 cells cultured on alendronate-immobilized Ti were down-regulated. Our study suggests that alendronate-immobilized Ti may be a bioactive implant with dual functions to enhance osteoblast differentiation and to inhibit osteoclast differentiation simultaneously.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Acid phosphatase (tartrate-resistant)</subject><subject>Alendronate</subject><subject>Alendronate - chemistry</subject><subject>Alendronate - pharmacology</subject><subject>Alendronic acid</subject><subject>ALKALINE PHOSPHATASE</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Bisphosphonates</subject><subject>BONDING</subject><subject>Bone implants</subject><subject>BONE TISSUES</subject><subject>Cathepsin K</subject><subject>CATHEPSINS</subject><subject>CELL CULTURES</subject><subject>Cell Line</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Coated Materials, Biocompatible - pharmacology</subject><subject>Coatings</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>Dental restorative materials</subject><subject>Gene expression</subject><subject>GRAFTS</subject><subject>HEPARIN</subject><subject>Heparin - chemistry</subject><subject>Heparin - pharmacology</subject><subject>INHIBITION</subject><subject>LIGANDS</subject><subject>MECHANICAL PROPERTIES</subject><subject>MESSENGER-RNA</subject><subject>METABOLISM</subject><subject>Mice</subject><subject>Orthopedics</subject><subject>Osteoblast</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts - drug effects</subject><subject>Osteocalcin</subject><subject>Osteoclast</subject><subject>Osteoclastogenesis</subject><subject>Osteoclasts</subject><subject>Osteoclasts - drug effects</subject><subject>Osteogenesis</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>RANK Ligand - pharmacology</subject><subject>SKELETON</subject><subject>Surface Properties</subject><subject>TITANIUM</subject><subject>Titanium - chemistry</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1TAQhS0EopfCH2CBIrEom4Sx83IkNqgqD6lSN0ViZzmTMddXiX2xHSSW_PM6vaXLemPL883RnDmMveVQceDdx0M1jgErAZxXICto-2dsx2GAUnBonrMdAHSlGPjPM_YqxgNksOmGl-xMcJnPIHfs35UxhKnwptjTUQfrCu2mQs_kpuCdTlSg18m6X4V3RbJJO7suRVyD0Uhx-7Rub0ebbH5mFR8TeZx1TPdC5PbaIS3k0mN1vK-a1eHW9Jq9MHqO9ObhPmc_vlzdXn4rr2--fr_8fF1iI0UqJZeN1IbqDnUt6rHuWxy1bAbUba-hlfUwYW8QTF5NwyXHkXfQAoz95hXqc_b-pJtnsCqiTYR79M5l-0oIAX0LPFMXJ-oY_O-VYlKLjUjzrB35NSo5tLLlopeZ_PAkyUHIrNm0XUbFCcXgYwxk1DHYRYe_GVJblOqgtijVFqUCqXKUuendg_46LjQ9tvzPLgOfTgDlrf2xFDZTlJc92bB5mrx9Sv8OxQ2wWw</recordid><startdate>20110923</startdate><enddate>20110923</enddate><creator>Moon, Ho-Jin</creator><creator>Yun, Young-Pil</creator><creator>Han, Choong-Wan</creator><creator>Kim, Min Sung</creator><creator>Kim, Sung Eun</creator><creator>Bae, Min Soo</creator><creator>Kim, Gyu-Tae</creator><creator>Choi, Yong-Suk</creator><creator>Hwang, Eui-Hwan</creator><creator>Lee, Joon Woo</creator><creator>Lee, Jin-Moo</creator><creator>Lee, Chang-Hoon</creator><creator>Kim, Duck-Su</creator><creator>Kwon, Il Keun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20110923</creationdate><title>Effect of heparin and alendronate coating on titanium surfaces on inhibition of osteoclast and enhancement of osteoblast function</title><author>Moon, Ho-Jin ; Yun, Young-Pil ; Han, Choong-Wan ; Kim, Min Sung ; Kim, Sung Eun ; Bae, Min Soo ; Kim, Gyu-Tae ; Choi, Yong-Suk ; Hwang, Eui-Hwan ; Lee, Joon Woo ; Lee, Jin-Moo ; Lee, Chang-Hoon ; Kim, Duck-Su ; Kwon, Il Keun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-81848afe36ca323b375cba849ca57a05839dc7fc0f0164181cb160500b7888803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Acid phosphatase (tartrate-resistant)</topic><topic>Alendronate</topic><topic>Alendronate - chemistry</topic><topic>Alendronate - pharmacology</topic><topic>Alendronic acid</topic><topic>ALKALINE PHOSPHATASE</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Bisphosphonates</topic><topic>BONDING</topic><topic>Bone implants</topic><topic>BONE TISSUES</topic><topic>Cathepsin K</topic><topic>CATHEPSINS</topic><topic>CELL CULTURES</topic><topic>Cell Line</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Coated Materials, Biocompatible - pharmacology</topic><topic>Coatings</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>Dental restorative materials</topic><topic>Gene expression</topic><topic>GRAFTS</topic><topic>HEPARIN</topic><topic>Heparin - chemistry</topic><topic>Heparin - pharmacology</topic><topic>INHIBITION</topic><topic>LIGANDS</topic><topic>MECHANICAL PROPERTIES</topic><topic>MESSENGER-RNA</topic><topic>METABOLISM</topic><topic>Mice</topic><topic>Orthopedics</topic><topic>Osteoblast</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts - drug effects</topic><topic>Osteocalcin</topic><topic>Osteoclast</topic><topic>Osteoclastogenesis</topic><topic>Osteoclasts</topic><topic>Osteoclasts - drug effects</topic><topic>Osteogenesis</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>RANK Ligand - pharmacology</topic><topic>SKELETON</topic><topic>Surface Properties</topic><topic>TITANIUM</topic><topic>Titanium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moon, Ho-Jin</creatorcontrib><creatorcontrib>Yun, Young-Pil</creatorcontrib><creatorcontrib>Han, Choong-Wan</creatorcontrib><creatorcontrib>Kim, Min Sung</creatorcontrib><creatorcontrib>Kim, Sung Eun</creatorcontrib><creatorcontrib>Bae, Min Soo</creatorcontrib><creatorcontrib>Kim, Gyu-Tae</creatorcontrib><creatorcontrib>Choi, Yong-Suk</creatorcontrib><creatorcontrib>Hwang, Eui-Hwan</creatorcontrib><creatorcontrib>Lee, Joon Woo</creatorcontrib><creatorcontrib>Lee, Jin-Moo</creatorcontrib><creatorcontrib>Lee, Chang-Hoon</creatorcontrib><creatorcontrib>Kim, Duck-Su</creatorcontrib><creatorcontrib>Kwon, Il Keun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moon, Ho-Jin</au><au>Yun, Young-Pil</au><au>Han, Choong-Wan</au><au>Kim, Min Sung</au><au>Kim, Sung Eun</au><au>Bae, Min Soo</au><au>Kim, Gyu-Tae</au><au>Choi, Yong-Suk</au><au>Hwang, Eui-Hwan</au><au>Lee, Joon Woo</au><au>Lee, Jin-Moo</au><au>Lee, Chang-Hoon</au><au>Kim, Duck-Su</au><au>Kwon, Il Keun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of heparin and alendronate coating on titanium surfaces on inhibition of osteoclast and enhancement of osteoblast function</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2011-09-23</date><risdate>2011</risdate><volume>413</volume><issue>2</issue><spage>194</spage><epage>200</epage><pages>194-200</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► We examine bone metabolism of engineered alendronate attached to Ti surfaces. ► Alendronate-immobilized Ti enhances activation of osteoblast differentiation. ► Alendronate-immobilized Ti inhibits osteoclast differentiation. ► Alendronate-immobilized Ti may be a bioactive implant with dual functions.
The failure of orthopedic and dental implants has been attributed mainly to loosening of the implant from host bone, which may be due to weak bonding of the implant material to bone tissue. Titanium (Ti) is used in the field of orthopedic and dental implants because of its excellent biocompatibility and outstanding mechanical properties. Therefore, in the field of materials science and tissue engineering, there has been extensive research to immobilize bioactive molecules on the surface of implant materials in order to provide the implants with improved adhesion to the host bone tissue.
In this study, chemically active functional groups were introduced on the surface of Ti by a grafting reaction with heparin and then the Ti was functionalized by immobilizing alendronate onto the heparin-grafted surface. In the MC3T3-E1 cell osteogenic differentiation study, the alendronate-immobilized Ti substrates significantly enhanced alkaline phosphatase activity (ALP) and calcium content. Additionally, nuclear factor kappa B ligand (RANKL)-induced osteoclast differentiation of RAW264.7 cells was inhibited with the alendronate-immobilized Ti as confirmed by TRAP analysis. Real time PCR analysis showed that mRNA expressions of osteocalcin and osteopontin, which are markers for osteogenesis, were upregulated in MC3T3-E1 cells cultured on alendronate-immobilized Ti. The mRNA expressions of TRAP and Cathepsin K, markers for osteoclastogenesis, in RAW264.7 cells cultured on alendronate-immobilized Ti were down-regulated. Our study suggests that alendronate-immobilized Ti may be a bioactive implant with dual functions to enhance osteoblast differentiation and to inhibit osteoclast differentiation simultaneously.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21888898</pmid><doi>10.1016/j.bbrc.2011.08.057</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2011-09, Vol.413 (2), p.194-200 |
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| subjects | 60 APPLIED LIFE SCIENCES Acid phosphatase (tartrate-resistant) Alendronate Alendronate - chemistry Alendronate - pharmacology Alendronic acid ALKALINE PHOSPHATASE Animals Biocompatibility Bisphosphonates BONDING Bone implants BONE TISSUES Cathepsin K CATHEPSINS CELL CULTURES Cell Line Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - pharmacology Coatings CONNECTIVE TISSUE CELLS Dental restorative materials Gene expression GRAFTS HEPARIN Heparin - chemistry Heparin - pharmacology INHIBITION LIGANDS MECHANICAL PROPERTIES MESSENGER-RNA METABOLISM Mice Orthopedics Osteoblast Osteoblastogenesis Osteoblasts - drug effects Osteocalcin Osteoclast Osteoclastogenesis Osteoclasts Osteoclasts - drug effects Osteogenesis POLYMERASE CHAIN REACTION RANK Ligand - pharmacology SKELETON Surface Properties TITANIUM Titanium - chemistry |
| title | Effect of heparin and alendronate coating on titanium surfaces on inhibition of osteoclast and enhancement of osteoblast function |
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