Soft matrix supports osteogenic differentiation of human dental follicle cells
► Rigid stiffness supports osteogenic differentiation in mesenchymal stem cells (MSCs). ► Our study examined stiffness and differentiation of dental follicle cells (DFCs). ► Soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs. ► DFCs and MSCs react contrarily to soft...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2011-07, Vol.410 (3), p.587-592 |
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| creator | Viale-Bouroncle, Sandra Völlner, Florian Möhl, Christoph Küpper, Kevin Brockhoff, Gero Reichert, Torsten E. Schmalz, Gottfried Morsczeck, Christian |
| description | ► Rigid stiffness supports osteogenic differentiation in mesenchymal stem cells (MSCs). ► Our study examined stiffness and differentiation of dental follicle cells (DFCs). ► Soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs. ► DFCs and MSCs react contrarily to soft and rigid surface stiffness.
The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs. |
| doi_str_mv | 10.1016/j.bbrc.2011.06.031 |
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The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2011.06.031</identifier><identifier>PMID: 21684253</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; BONE MARROW ; Cell culture ; Cell Culture Techniques ; CELL CULTURES ; CELL DIFFERENTIATION ; CELL PROLIFERATION ; Cell surface ; Cells, Cultured ; Culture Media - pharmacology ; Dental follicle cells ; Dental Sac - cytology ; Dental Sac - drug effects ; DEXAMETHASONE ; Dexamethasone - pharmacology ; Differentiation ; Extracellular matrix ; FLEXIBILITY ; Follicles ; Humans ; Male ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Mesenchyme ; Osteogenesis ; PDMS ; Proliferation ; SKELETON ; STEM CELLS ; Surface Properties ; Young Adult</subject><ispartof>Biochemical and biophysical research communications, 2011-07, Vol.410 (3), p.587-592</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-1eaa8bf0fb2443cf4a067aac24c521e4f0fd9f9a53665addbd681edca3387b103</citedby><cites>FETCH-LOGICAL-c416t-1eaa8bf0fb2443cf4a067aac24c521e4f0fd9f9a53665addbd681edca3387b103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2011.06.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21684253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22204985$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Viale-Bouroncle, Sandra</creatorcontrib><creatorcontrib>Völlner, Florian</creatorcontrib><creatorcontrib>Möhl, Christoph</creatorcontrib><creatorcontrib>Küpper, Kevin</creatorcontrib><creatorcontrib>Brockhoff, Gero</creatorcontrib><creatorcontrib>Reichert, Torsten E.</creatorcontrib><creatorcontrib>Schmalz, Gottfried</creatorcontrib><creatorcontrib>Morsczeck, Christian</creatorcontrib><title>Soft matrix supports osteogenic differentiation of human dental follicle cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► Rigid stiffness supports osteogenic differentiation in mesenchymal stem cells (MSCs). ► Our study examined stiffness and differentiation of dental follicle cells (DFCs). ► Soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs. ► DFCs and MSCs react contrarily to soft and rigid surface stiffness.
The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>BONE MARROW</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>CELL CULTURES</subject><subject>CELL DIFFERENTIATION</subject><subject>CELL PROLIFERATION</subject><subject>Cell surface</subject><subject>Cells, Cultured</subject><subject>Culture Media - pharmacology</subject><subject>Dental follicle cells</subject><subject>Dental Sac - cytology</subject><subject>Dental Sac - drug effects</subject><subject>DEXAMETHASONE</subject><subject>Dexamethasone - pharmacology</subject><subject>Differentiation</subject><subject>Extracellular matrix</subject><subject>FLEXIBILITY</subject><subject>Follicles</subject><subject>Humans</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchyme</subject><subject>Osteogenesis</subject><subject>PDMS</subject><subject>Proliferation</subject><subject>SKELETON</subject><subject>STEM CELLS</subject><subject>Surface Properties</subject><subject>Young Adult</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1TAQhq0KRA-FF-gCWWIBm6Rjx3ESqRtUcZMqWBQkdpZjj6mPkvjUdhB9-zo6hWVXI81888_lJ-ScQc2AyYt9PY7R1BwYq0HW0LATsmMwQMUZiGdkBwCy4gP7dUpeprSHAgo5vCCnnMle8LbZkW83wWU66xz9X5rWwyHEnGhIGcNvXLyh1juHEZfsdfZhocHR23XWC7UlpyfqwjR5MyE1OE3pFXnu9JTw9WM8Iz8_ffxx9aW6_v7569WH68oIJnPFUOt-dOBGLkRjnNAgO60NF6blDEWp2MENum2kbLW1o5U9Q2t00_TdyKA5I2-PumVTr5LxGc2tCcuCJivOOYihbwv17kgdYrhbMWU1-7TtqRcMa1J9J1nfd7wr5PsnyfLvbmhbNmyj-RE1MaQU0alD9LOO9wXaOKn2avNFbb4okKr4UprePOqv44z2f8s_IwpweQSwfO2Px7gdhYtB6-N2kw3-Kf0HRdOfAA</recordid><startdate>20110708</startdate><enddate>20110708</enddate><creator>Viale-Bouroncle, Sandra</creator><creator>Völlner, Florian</creator><creator>Möhl, Christoph</creator><creator>Küpper, Kevin</creator><creator>Brockhoff, Gero</creator><creator>Reichert, Torsten E.</creator><creator>Schmalz, Gottfried</creator><creator>Morsczeck, Christian</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20110708</creationdate><title>Soft matrix supports osteogenic differentiation of human dental follicle cells</title><author>Viale-Bouroncle, Sandra ; 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The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21684253</pmid><doi>10.1016/j.bbrc.2011.06.031</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2011-07, Vol.410 (3), p.587-592 |
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| subjects | 60 APPLIED LIFE SCIENCES BONE MARROW Cell culture Cell Culture Techniques CELL CULTURES CELL DIFFERENTIATION CELL PROLIFERATION Cell surface Cells, Cultured Culture Media - pharmacology Dental follicle cells Dental Sac - cytology Dental Sac - drug effects DEXAMETHASONE Dexamethasone - pharmacology Differentiation Extracellular matrix FLEXIBILITY Follicles Humans Male Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchyme Osteogenesis PDMS Proliferation SKELETON STEM CELLS Surface Properties Young Adult |
| title | Soft matrix supports osteogenic differentiation of human dental follicle cells |
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