Adhesion-mediated self-renewal abilities of Ph+ blastoma cells
The Philadelphia chromosome-positive blastoma, maintained by serial subcutaneous transplantation in nude mice, is a highly proliferating biological mass consisting of homogenous CD34 +CD38 − myeloblastoid cells. These cells newly evolved from pluripotent leukemia stem cells of chronic myeloid leukem...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2010-05, Vol.396 (2), p.193-198 |
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| creator | Funayama, Keiji Saito-Kurimoto, Yumi Ebihara, Yasuhiro Shimane, Miyuki Nomura, Hitoshi Tsuji, Ko-ichiro Asano, Shigetaka |
| description | The Philadelphia chromosome-positive blastoma, maintained by serial subcutaneous transplantation in nude mice, is a highly proliferating biological mass consisting of homogenous CD34
+CD38
− myeloblastoid cells. These cells newly evolved from pluripotent leukemia stem cells of chronic myeloid leukemia in the chronic phase. Therefore, this mass may provide a unique tool for better understanding cellular and molecular mechanisms of self-renewal of leukemia stem cells. In this paper, we demonstrated that intravenously injected blastoma cells can cause Ph+ blastic leukemia with multiple invasive foci in NOD/SCID mice but not in nude mice. In addition, using an in vitro culture system, we clearly showed that blastoma cell adhesion to OP9 stromal cells accelerates blastoma cell proliferation that is associated with up-regulation of BMI1 gene expression; increased levels of β-catenin and the Notch1 intra-cellular domain; and changed the expression pattern of variant CD44 forms, which are constitutively expressed in these blastoma cells. These findings strongly suggest that adhesion of leukemic stem cells to stromal cells via CD44 might be indispensable for their cellular defense against attack by immune cells and for maintenance of their self-renewal ability. |
| doi_str_mv | 10.1016/j.bbrc.2010.03.172 |
| format | Article |
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− myeloblastoid cells. These cells newly evolved from pluripotent leukemia stem cells of chronic myeloid leukemia in the chronic phase. Therefore, this mass may provide a unique tool for better understanding cellular and molecular mechanisms of self-renewal of leukemia stem cells. In this paper, we demonstrated that intravenously injected blastoma cells can cause Ph+ blastic leukemia with multiple invasive foci in NOD/SCID mice but not in nude mice. In addition, using an in vitro culture system, we clearly showed that blastoma cell adhesion to OP9 stromal cells accelerates blastoma cell proliferation that is associated with up-regulation of BMI1 gene expression; increased levels of β-catenin and the Notch1 intra-cellular domain; and changed the expression pattern of variant CD44 forms, which are constitutively expressed in these blastoma cells. These findings strongly suggest that adhesion of leukemic stem cells to stromal cells via CD44 might be indispensable for their cellular defense against attack by immune cells and for maintenance of their self-renewal ability.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2010.03.172</identifier><identifier>PMID: 20362549</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; beta Catenin - metabolism ; Blastoma ; Cell Adhesion ; Cell Culture Techniques ; CELL PROLIFERATION ; Chronic myeloid leukemia ; Gene Expression Regulation, Leukemic ; GENE REGULATION ; Humans ; Hyaluronan Receptors - genetics ; Hyaluronan Receptors - metabolism ; IN VITRO ; Leukemic stem cell ; MICE ; Mice, Inbred NOD ; Mice, SCID ; MYELOID LEUKEMIA ; Neoplasm Transplantation ; Neoplastic Stem Cells - pathology ; Nuclear Proteins - genetics ; PH VALUE ; PHILADELPHIA CHROMOSOME ; Pluripotent Stem Cells - pathology ; Polycomb Repressive Complex 1 ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology ; Proto-Oncogene Proteins - genetics ; Receptor, Notch1 - metabolism ; Repressor Proteins - genetics ; Self-renewal ability ; STEM CELLS</subject><ispartof>Biochemical and biophysical research communications, 2010-05, Vol.396 (2), p.193-198</ispartof><rights>2010 Elsevier Inc.</rights><rights>Copyright (c) 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c298t-9c044c98ebb9d68783c1efa9647521cf1c4709b5408e2a3835b4272d3b1d22473</citedby><cites>FETCH-LOGICAL-c298t-9c044c98ebb9d68783c1efa9647521cf1c4709b5408e2a3835b4272d3b1d22473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X10006479$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20362549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22202607$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Funayama, Keiji</creatorcontrib><creatorcontrib>Saito-Kurimoto, Yumi</creatorcontrib><creatorcontrib>Ebihara, Yasuhiro</creatorcontrib><creatorcontrib>Shimane, Miyuki</creatorcontrib><creatorcontrib>Nomura, Hitoshi</creatorcontrib><creatorcontrib>Tsuji, Ko-ichiro</creatorcontrib><creatorcontrib>Asano, Shigetaka</creatorcontrib><title>Adhesion-mediated self-renewal abilities of Ph+ blastoma cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The Philadelphia chromosome-positive blastoma, maintained by serial subcutaneous transplantation in nude mice, is a highly proliferating biological mass consisting of homogenous CD34
+CD38
− myeloblastoid cells. These cells newly evolved from pluripotent leukemia stem cells of chronic myeloid leukemia in the chronic phase. Therefore, this mass may provide a unique tool for better understanding cellular and molecular mechanisms of self-renewal of leukemia stem cells. In this paper, we demonstrated that intravenously injected blastoma cells can cause Ph+ blastic leukemia with multiple invasive foci in NOD/SCID mice but not in nude mice. In addition, using an in vitro culture system, we clearly showed that blastoma cell adhesion to OP9 stromal cells accelerates blastoma cell proliferation that is associated with up-regulation of BMI1 gene expression; increased levels of β-catenin and the Notch1 intra-cellular domain; and changed the expression pattern of variant CD44 forms, which are constitutively expressed in these blastoma cells. These findings strongly suggest that adhesion of leukemic stem cells to stromal cells via CD44 might be indispensable for their cellular defense against attack by immune cells and for maintenance of their self-renewal ability.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>beta Catenin - metabolism</subject><subject>Blastoma</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques</subject><subject>CELL PROLIFERATION</subject><subject>Chronic myeloid leukemia</subject><subject>Gene Expression Regulation, Leukemic</subject><subject>GENE REGULATION</subject><subject>Humans</subject><subject>Hyaluronan Receptors - genetics</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>IN VITRO</subject><subject>Leukemic stem cell</subject><subject>MICE</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>MYELOID LEUKEMIA</subject><subject>Neoplasm Transplantation</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Nuclear Proteins - genetics</subject><subject>PH VALUE</subject><subject>PHILADELPHIA CHROMOSOME</subject><subject>Pluripotent Stem Cells - pathology</subject><subject>Polycomb Repressive Complex 1</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Receptor, Notch1 - metabolism</subject><subject>Repressor Proteins - genetics</subject><subject>Self-renewal ability</subject><subject>STEM CELLS</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMoOj7-gAspuHAhHW9uMm0DIoj4AkEXCu5Ckt4yGTqNJh3Ff2_LqEtXFy7fORw-xg45TDnw4mwxtTa6KcLwADHlJW6wCQcFOXKQm2wCAEWOir_usN2UFgCcy0Jtsx0EUeBMqgm7uKznlHzo8iXV3vRUZ4naJo_U0adpM2N963tPKQtN9jQ_zWxrUh-WJnPUtmmfbTWmTXTwc_fYy83189Vd_vB4e391-ZA7VFWfKwdSOlWRtaouqrISjlNjVCHLGXLXcCdLUHYmoSI0ohIzK7HEWlheI8pS7LHjdW9IvdfJ-Z7c3IWuI9drRAQsYKRO1tRbDO8rSr1e-jTuNB2FVdKlELySUuJA4pp0MaQUqdFv0S9N_NIc9ChXL_QoV49yNQg9yB1CRz_1KzvY-ov82hyA8zVAg4oPT3FcSp0bzMZxaB38f_3fkYeISQ</recordid><startdate>20100528</startdate><enddate>20100528</enddate><creator>Funayama, Keiji</creator><creator>Saito-Kurimoto, Yumi</creator><creator>Ebihara, Yasuhiro</creator><creator>Shimane, Miyuki</creator><creator>Nomura, Hitoshi</creator><creator>Tsuji, Ko-ichiro</creator><creator>Asano, Shigetaka</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20100528</creationdate><title>Adhesion-mediated self-renewal abilities of Ph+ blastoma cells</title><author>Funayama, Keiji ; Saito-Kurimoto, Yumi ; Ebihara, Yasuhiro ; Shimane, Miyuki ; Nomura, Hitoshi ; Tsuji, Ko-ichiro ; Asano, Shigetaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-9c044c98ebb9d68783c1efa9647521cf1c4709b5408e2a3835b4272d3b1d22473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>beta Catenin - metabolism</topic><topic>Blastoma</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques</topic><topic>CELL PROLIFERATION</topic><topic>Chronic myeloid leukemia</topic><topic>Gene Expression Regulation, Leukemic</topic><topic>GENE REGULATION</topic><topic>Humans</topic><topic>Hyaluronan Receptors - genetics</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>IN VITRO</topic><topic>Leukemic stem cell</topic><topic>MICE</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>MYELOID LEUKEMIA</topic><topic>Neoplasm Transplantation</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Nuclear Proteins - genetics</topic><topic>PH VALUE</topic><topic>PHILADELPHIA CHROMOSOME</topic><topic>Pluripotent Stem Cells - pathology</topic><topic>Polycomb Repressive Complex 1</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Receptor, Notch1 - metabolism</topic><topic>Repressor Proteins - genetics</topic><topic>Self-renewal ability</topic><topic>STEM CELLS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Funayama, Keiji</creatorcontrib><creatorcontrib>Saito-Kurimoto, Yumi</creatorcontrib><creatorcontrib>Ebihara, Yasuhiro</creatorcontrib><creatorcontrib>Shimane, Miyuki</creatorcontrib><creatorcontrib>Nomura, Hitoshi</creatorcontrib><creatorcontrib>Tsuji, Ko-ichiro</creatorcontrib><creatorcontrib>Asano, Shigetaka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Funayama, Keiji</au><au>Saito-Kurimoto, Yumi</au><au>Ebihara, Yasuhiro</au><au>Shimane, Miyuki</au><au>Nomura, Hitoshi</au><au>Tsuji, Ko-ichiro</au><au>Asano, Shigetaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adhesion-mediated self-renewal abilities of Ph+ blastoma cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2010-05-28</date><risdate>2010</risdate><volume>396</volume><issue>2</issue><spage>193</spage><epage>198</epage><pages>193-198</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The Philadelphia chromosome-positive blastoma, maintained by serial subcutaneous transplantation in nude mice, is a highly proliferating biological mass consisting of homogenous CD34
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− myeloblastoid cells. These cells newly evolved from pluripotent leukemia stem cells of chronic myeloid leukemia in the chronic phase. Therefore, this mass may provide a unique tool for better understanding cellular and molecular mechanisms of self-renewal of leukemia stem cells. In this paper, we demonstrated that intravenously injected blastoma cells can cause Ph+ blastic leukemia with multiple invasive foci in NOD/SCID mice but not in nude mice. In addition, using an in vitro culture system, we clearly showed that blastoma cell adhesion to OP9 stromal cells accelerates blastoma cell proliferation that is associated with up-regulation of BMI1 gene expression; increased levels of β-catenin and the Notch1 intra-cellular domain; and changed the expression pattern of variant CD44 forms, which are constitutively expressed in these blastoma cells. These findings strongly suggest that adhesion of leukemic stem cells to stromal cells via CD44 might be indispensable for their cellular defense against attack by immune cells and for maintenance of their self-renewal ability.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20362549</pmid><doi>10.1016/j.bbrc.2010.03.172</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2010-05, Vol.396 (2), p.193-198 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 60 APPLIED LIFE SCIENCES Animals beta Catenin - metabolism Blastoma Cell Adhesion Cell Culture Techniques CELL PROLIFERATION Chronic myeloid leukemia Gene Expression Regulation, Leukemic GENE REGULATION Humans Hyaluronan Receptors - genetics Hyaluronan Receptors - metabolism IN VITRO Leukemic stem cell MICE Mice, Inbred NOD Mice, SCID MYELOID LEUKEMIA Neoplasm Transplantation Neoplastic Stem Cells - pathology Nuclear Proteins - genetics PH VALUE PHILADELPHIA CHROMOSOME Pluripotent Stem Cells - pathology Polycomb Repressive Complex 1 Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology Proto-Oncogene Proteins - genetics Receptor, Notch1 - metabolism Repressor Proteins - genetics Self-renewal ability STEM CELLS |
| title | Adhesion-mediated self-renewal abilities of Ph+ blastoma cells |
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