Characterization of mouse UDP-glucose pyrophosphatase, a Nudix hydrolase encoded by the Nudt14 gene

Recombinant mouse UDP-glucose pyrophosphatase (UGPPase), encoded by the Nudt14 gene, was produced in Escherichia coli and purified close to homogeneity. The enzyme catalyzed the conversion of [β- 32P]UDP-glucose to [ 32P]glucose-1-P and UMP, confirming that it hydrolyzed the pyrophosphate of the nuc...

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Veröffentlicht in:	Biochemical and biophysical research communications 2009-12, Vol.390 (4), p.1414-1418
Hauptverfasser:	Heyen, Candy A., Tagliabracci, Vincent S., Zhai, Lanmin, Roach, Peter J.
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES ADP ADP-ribose ANIMAL TISSUES Animals BRAIN Caenorhabditis elegans Catalysis Cloning, Molecular DIMERIZATION DROSOPHILA Drosophila melanogaster ENZYMES ESCHERICHIA COLI GENES GLUCOSE Glucosephosphates - metabolism GLYCOGEN HEART KIDNEYS LIVER LUNGS MAGNESIUM IONS MICE MONOMERS MUSCLES NUCLEOSIDES Nudix hydrolase Nudix Hydrolases Nudt14 PANCREAS PH VALUE PHOSPHATES Phylogeny Protein Multimerization Pyrophosphatase Pyrophosphatases - chemistry Pyrophosphatases - classification Pyrophosphatases - genetics Pyrophosphatases - metabolism Recombinant Proteins - classification Recombinant Proteins - genetics Recombinant Proteins - metabolism RIBOSE SACCHAROSE Tissue Distribution UDP-glucose Uridine Diphosphate Glucose - metabolism
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