Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 k
The visible fluorescence spectrum of single flavoprotein at a temperature of 1.5 K has been measured by one-photon excitation. The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 1...
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| Veröffentlicht in: | Physical review letters 2011-02, Vol.106 (7), p.078101-078101, Article 078101 |
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| container_end_page | 078101 |
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| container_issue | 7 |
| container_start_page | 078101 |
| container_title | Physical review letters |
| container_volume | 106 |
| creator | Fujiyoshi, Satoru Hirano, Mitsuharu Matsushita, Michio Iseki, Mineo Watanabe, Masakatsu |
| description | The visible fluorescence spectrum of single flavoprotein at a temperature of 1.5 K has been measured by one-photon excitation. The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 10(-3) s(-1) around hydrogen bonds at the flavin cofactor binding site. |
| doi_str_mv | 10.1103/physrevlett.106.078101 |
| format | Article |
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The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 10(-3) s(-1) around hydrogen bonds at the flavin cofactor binding site.</description><subject>Binding Sites</subject><subject>BOSONS</subject><subject>CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS</subject><subject>ELEMENTARY PARTICLES</subject><subject>ELEMENTS</subject><subject>EMISSION SPECTROSCOPY</subject><subject>ENERGY-LEVEL TRANSITIONS</subject><subject>Euglena gracilis</subject><subject>EXCITATION</subject><subject>Flavins - metabolism</subject><subject>Flavoproteins - chemistry</subject><subject>Flavoproteins - metabolism</subject><subject>FLUORESCENCE SPECTROSCOPY</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>HYDROGEN</subject><subject>Hydrogen Bonding</subject><subject>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</subject><subject>ISOALLOXAZINES</subject><subject>MASSLESS PARTICLES</subject><subject>Models, Molecular</subject><subject>NONMETALS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>ORGANIC OXYGEN COMPOUNDS</subject><subject>PHOTONS</subject><subject>Protein Structure, Tertiary</subject><subject>PROTEINS</subject><subject>Protozoan Proteins - chemistry</subject><subject>Protozoan Proteins - metabolism</subject><subject>SPECTRA</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>SPECTROSCOPY</subject><subject>Temperature</subject><subject>TEMPERATURE RANGE</subject><subject>TEMPERATURE RANGE 0000-0013 K</subject><issn>0031-9007</issn><issn>1079-7114</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kduO0zAQhi0EYruFV1hZ4oKrlBk7id1LtOIkVQJxuLYcZ9IGUjvYTqWKl-FZeDLMdpcrS55vDr8-xm4QNoggX82Hc4p0mijnDUK7AaUR8BFbIahtpRDrx2wFILHaAqgrdp3SdwBA0eqn7EpgDU1T1yv260uOi8tLtBN3B-v3xMPALXdhsC6HyLvR96Pf8zTmu9Iw2VOYY8g0et5TJpep5925AH4_UfVQGqYlREqOvCOe5oLFkFyYz9xmjpvmz-8fz9iTwU6Jnt-_a_bt7Zuvt--r3cd3H25f7yrXAOZKKqdJWOy2Cp1tOjsgkpDYyRp6oQClUD05ofsaWtcr2FrbN84KjaIhreWavbjMDSmPJrmSxB1c8L4cZQQ2sgxThXp5oUqCnwulbI5jOX-arKewJKMbVWupy7o1ay-kK5GKhcHMcTzaeDYI5p8d86nY-UynXbFT_lpzsVMab-5XLN2R-v9tDzrkX60ekJk</recordid><startdate>20110218</startdate><enddate>20110218</enddate><creator>Fujiyoshi, Satoru</creator><creator>Hirano, Mitsuharu</creator><creator>Matsushita, Michio</creator><creator>Iseki, Mineo</creator><creator>Watanabe, Masakatsu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20110218</creationdate><title>Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 k</title><author>Fujiyoshi, Satoru ; Hirano, Mitsuharu ; Matsushita, Michio ; Iseki, Mineo ; Watanabe, Masakatsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-37c8e2a1b971ca5baf11e231b340d2701327dec28d406cd709aad5ca28125e883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Binding Sites</topic><topic>BOSONS</topic><topic>CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS</topic><topic>ELEMENTARY PARTICLES</topic><topic>ELEMENTS</topic><topic>EMISSION SPECTROSCOPY</topic><topic>ENERGY-LEVEL TRANSITIONS</topic><topic>Euglena gracilis</topic><topic>EXCITATION</topic><topic>Flavins - metabolism</topic><topic>Flavoproteins - chemistry</topic><topic>Flavoproteins - metabolism</topic><topic>FLUORESCENCE SPECTROSCOPY</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>HYDROGEN</topic><topic>Hydrogen Bonding</topic><topic>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</topic><topic>ISOALLOXAZINES</topic><topic>MASSLESS PARTICLES</topic><topic>Models, Molecular</topic><topic>NONMETALS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>ORGANIC OXYGEN COMPOUNDS</topic><topic>PHOTONS</topic><topic>Protein Structure, Tertiary</topic><topic>PROTEINS</topic><topic>Protozoan Proteins - chemistry</topic><topic>Protozoan Proteins - metabolism</topic><topic>SPECTRA</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>SPECTROSCOPY</topic><topic>Temperature</topic><topic>TEMPERATURE RANGE</topic><topic>TEMPERATURE RANGE 0000-0013 K</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujiyoshi, Satoru</creatorcontrib><creatorcontrib>Hirano, Mitsuharu</creatorcontrib><creatorcontrib>Matsushita, Michio</creatorcontrib><creatorcontrib>Iseki, Mineo</creatorcontrib><creatorcontrib>Watanabe, Masakatsu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Physical review letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujiyoshi, Satoru</au><au>Hirano, Mitsuharu</au><au>Matsushita, Michio</au><au>Iseki, Mineo</au><au>Watanabe, Masakatsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 k</atitle><jtitle>Physical review letters</jtitle><addtitle>Phys Rev Lett</addtitle><date>2011-02-18</date><risdate>2011</risdate><volume>106</volume><issue>7</issue><spage>078101</spage><epage>078101</epage><pages>078101-078101</pages><artnum>078101</artnum><issn>0031-9007</issn><eissn>1079-7114</eissn><abstract>The visible fluorescence spectrum of single flavoprotein at a temperature of 1.5 K has been measured by one-photon excitation. The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 10(-3) s(-1) around hydrogen bonds at the flavin cofactor binding site.</abstract><cop>United States</cop><pmid>21405544</pmid><doi>10.1103/physrevlett.106.078101</doi><tpages>1</tpages></addata></record> |
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| identifier | ISSN: 0031-9007 |
| ispartof | Physical review letters, 2011-02, Vol.106 (7), p.078101-078101, Article 078101 |
| issn | 0031-9007 1079-7114 |
| language | eng |
| recordid | cdi_osti_scitechconnect_21532317 |
| source | MEDLINE; American Physical Society Journals |
| subjects | Binding Sites BOSONS CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS ELEMENTARY PARTICLES ELEMENTS EMISSION SPECTROSCOPY ENERGY-LEVEL TRANSITIONS Euglena gracilis EXCITATION Flavins - metabolism Flavoproteins - chemistry Flavoproteins - metabolism FLUORESCENCE SPECTROSCOPY HETEROCYCLIC COMPOUNDS HYDROGEN Hydrogen Bonding INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ISOALLOXAZINES MASSLESS PARTICLES Models, Molecular NONMETALS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS ORGANIC OXYGEN COMPOUNDS PHOTONS Protein Structure, Tertiary PROTEINS Protozoan Proteins - chemistry Protozoan Proteins - metabolism SPECTRA Spectrometry, Fluorescence - methods SPECTROSCOPY Temperature TEMPERATURE RANGE TEMPERATURE RANGE 0000-0013 K |
| title | Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 k |
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