The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells
Using a high-resolution, automated confocal high-content imaging system, we investigated the sub-cellular localization of the Serine 2481-autophosphorylated form of mTOR (PP-mTOR Ser2481) during mitosis and cytokinesis in human cancer cells. PP-mTOR Ser2481 exhibited a punctate nuclear distribution...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2009-03, Vol.380 (3), p.638-643 |
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| creator | Vazquez-Martin, Alejandro Oliveras-Ferraros, Cristina Bernadó, Luis López-Bonet, Eugeni Menendez, Javier A. |
| description | Using a high-resolution, automated confocal high-content imaging system, we investigated the sub-cellular localization of the Serine 2481-autophosphorylated form of mTOR (PP-mTOR
Ser2481) during mitosis and cytokinesis in human cancer cells. PP-mTOR
Ser2481 exhibited a punctate nuclear distribution in interphase cancer cells, with the number of PP-mTOR
Ser2481 nuclear speckles positively relating with the proliferative capacity of cancer cells. PP-mTOR
Ser2481 expression dynamically rearranged within the cytoplasm in a close association near and between separating chromosomes during early stages of mitosis. Towards the end of anaphase and in telophase, PP-mTOR
Ser2481 drastically focused on the midzone and ultimately in the centre of the midbody at the presumptive cleavage furrow. In cells at cytokinesis, PP-mTOR
Ser2481 appeared as a doublet facing each other at the apical ends of two daughter cells. Three-dimensional analysis confirmed that PP-mTOR
Ser2481 positioned at a ring structure wrapped round by microtubule bundles to connect daughter cells. These results reveal for the first time that PP-mTOR
Ser2481 may be unexpectedly involved in the terminal stages of cytokinesis. |
| doi_str_mv | 10.1016/j.bbrc.2009.01.153 |
| format | Article |
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Ser2481) during mitosis and cytokinesis in human cancer cells. PP-mTOR
Ser2481 exhibited a punctate nuclear distribution in interphase cancer cells, with the number of PP-mTOR
Ser2481 nuclear speckles positively relating with the proliferative capacity of cancer cells. PP-mTOR
Ser2481 expression dynamically rearranged within the cytoplasm in a close association near and between separating chromosomes during early stages of mitosis. Towards the end of anaphase and in telophase, PP-mTOR
Ser2481 drastically focused on the midzone and ultimately in the centre of the midbody at the presumptive cleavage furrow. In cells at cytokinesis, PP-mTOR
Ser2481 appeared as a doublet facing each other at the apical ends of two daughter cells. Three-dimensional analysis confirmed that PP-mTOR
Ser2481 positioned at a ring structure wrapped round by microtubule bundles to connect daughter cells. These results reveal for the first time that PP-mTOR
Ser2481 may be unexpectedly involved in the terminal stages of cytokinesis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2009.01.153</identifier><identifier>PMID: 19285014</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Cancer ; Carrier Proteins - metabolism ; CELL CYCLE ; Cell Division ; Cell Line, Tumor ; Cell Nucleus - enzymology ; Cell Proliferation ; Chromosomal passenger protein ; CHROMOSOMES ; Cytokinesis ; CYTOPLASM ; HUMAN POPULATIONS ; Humans ; MICROTUBULES ; MITOSIS ; mTOR ; NEOPLASMS ; Neoplasms - metabolism ; Neoplasms - pathology ; Phosphorylation ; Phosphotransferases (Alcohol Group Acceptor) - metabolism ; PROTEINS ; SERINE ; Serine - metabolism ; TOR Serine-Threonine Kinases</subject><ispartof>Biochemical and biophysical research communications, 2009-03, Vol.380 (3), p.638-643</ispartof><rights>2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-e9fc9d405ed1cda456ddf53f3f892c0b8dd8fd7f09385302630d04cb33a27b6a3</citedby><cites>FETCH-LOGICAL-c448t-e9fc9d405ed1cda456ddf53f3f892c0b8dd8fd7f09385302630d04cb33a27b6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2009.01.153$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19285014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/21255930$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Vazquez-Martin, Alejandro</creatorcontrib><creatorcontrib>Oliveras-Ferraros, Cristina</creatorcontrib><creatorcontrib>Bernadó, Luis</creatorcontrib><creatorcontrib>López-Bonet, Eugeni</creatorcontrib><creatorcontrib>Menendez, Javier A.</creatorcontrib><title>The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Using a high-resolution, automated confocal high-content imaging system, we investigated the sub-cellular localization of the Serine 2481-autophosphorylated form of mTOR (PP-mTOR
Ser2481) during mitosis and cytokinesis in human cancer cells. PP-mTOR
Ser2481 exhibited a punctate nuclear distribution in interphase cancer cells, with the number of PP-mTOR
Ser2481 nuclear speckles positively relating with the proliferative capacity of cancer cells. PP-mTOR
Ser2481 expression dynamically rearranged within the cytoplasm in a close association near and between separating chromosomes during early stages of mitosis. Towards the end of anaphase and in telophase, PP-mTOR
Ser2481 drastically focused on the midzone and ultimately in the centre of the midbody at the presumptive cleavage furrow. In cells at cytokinesis, PP-mTOR
Ser2481 appeared as a doublet facing each other at the apical ends of two daughter cells. Three-dimensional analysis confirmed that PP-mTOR
Ser2481 positioned at a ring structure wrapped round by microtubule bundles to connect daughter cells. These results reveal for the first time that PP-mTOR
Ser2481 may be unexpectedly involved in the terminal stages of cytokinesis.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Cancer</subject><subject>Carrier Proteins - metabolism</subject><subject>CELL CYCLE</subject><subject>Cell Division</subject><subject>Cell Line, Tumor</subject><subject>Cell Nucleus - enzymology</subject><subject>Cell Proliferation</subject><subject>Chromosomal passenger protein</subject><subject>CHROMOSOMES</subject><subject>Cytokinesis</subject><subject>CYTOPLASM</subject><subject>HUMAN POPULATIONS</subject><subject>Humans</subject><subject>MICROTUBULES</subject><subject>MITOSIS</subject><subject>mTOR</subject><subject>NEOPLASMS</subject><subject>Neoplasms - metabolism</subject><subject>Neoplasms - pathology</subject><subject>Phosphorylation</subject><subject>Phosphotransferases (Alcohol Group Acceptor) - metabolism</subject><subject>PROTEINS</subject><subject>SERINE</subject><subject>Serine - metabolism</subject><subject>TOR Serine-Threonine Kinases</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-LFDEQxYMo7rj6BTxIQBA9dFtJuns64EUW_8HCwDKCt5BOKjsZOp0x6VmY_Qx-aNPMgDcPRdXh9x6PeoS8ZlAzYN3HfT0MydQcQNbAataKJ2TFQELFGTRPyQoAuopL9uuKvMh5D8BY08nn5IpJ3rfAmhX5s90hzZj8hJQ3Pav0cY6HXcxl0mnUM1rqYgo0Ohp0CHr0eqJbne5xphtH7_RBh5PxE30ftpu7D9RnOkZTsMeinCMN3j7GYq4nu9xDtCdaaOsfvPXTPTV6MpiowXHML8kzp8eMry77mvz8-mV787263Xz7cfP5tjJN088VSmekbaBFy4zVTdtZ61rhhOslNzD01vbOrh1I0bcCeCfAQmMGITRfD50W1-Tt2Tfm2ats_IxmZ-I0oZkVZ7xtpYBCvTtThxR_HzHPKvi85NQTxmNW3RrEWrR9AfkZNCnmnNCpQ_JBp5NioJam1F4tTamlKQVMlaaK6M3F_TgEtP8kl2oK8OkMYPnEg8e0BMXyLOvTktNG_z__v0kJpVs</recordid><startdate>20090313</startdate><enddate>20090313</enddate><creator>Vazquez-Martin, Alejandro</creator><creator>Oliveras-Ferraros, Cristina</creator><creator>Bernadó, Luis</creator><creator>López-Bonet, Eugeni</creator><creator>Menendez, Javier A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20090313</creationdate><title>The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells</title><author>Vazquez-Martin, Alejandro ; Oliveras-Ferraros, Cristina ; Bernadó, Luis ; López-Bonet, Eugeni ; Menendez, Javier A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-e9fc9d405ed1cda456ddf53f3f892c0b8dd8fd7f09385302630d04cb33a27b6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Cancer</topic><topic>Carrier Proteins - metabolism</topic><topic>CELL CYCLE</topic><topic>Cell Division</topic><topic>Cell Line, Tumor</topic><topic>Cell Nucleus - enzymology</topic><topic>Cell Proliferation</topic><topic>Chromosomal passenger protein</topic><topic>CHROMOSOMES</topic><topic>Cytokinesis</topic><topic>CYTOPLASM</topic><topic>HUMAN POPULATIONS</topic><topic>Humans</topic><topic>MICROTUBULES</topic><topic>MITOSIS</topic><topic>mTOR</topic><topic>NEOPLASMS</topic><topic>Neoplasms - metabolism</topic><topic>Neoplasms - pathology</topic><topic>Phosphorylation</topic><topic>Phosphotransferases (Alcohol Group Acceptor) - metabolism</topic><topic>PROTEINS</topic><topic>SERINE</topic><topic>Serine - metabolism</topic><topic>TOR Serine-Threonine Kinases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vazquez-Martin, Alejandro</creatorcontrib><creatorcontrib>Oliveras-Ferraros, Cristina</creatorcontrib><creatorcontrib>Bernadó, Luis</creatorcontrib><creatorcontrib>López-Bonet, Eugeni</creatorcontrib><creatorcontrib>Menendez, Javier A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vazquez-Martin, Alejandro</au><au>Oliveras-Ferraros, Cristina</au><au>Bernadó, Luis</au><au>López-Bonet, Eugeni</au><au>Menendez, Javier A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2009-03-13</date><risdate>2009</risdate><volume>380</volume><issue>3</issue><spage>638</spage><epage>643</epage><pages>638-643</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Using a high-resolution, automated confocal high-content imaging system, we investigated the sub-cellular localization of the Serine 2481-autophosphorylated form of mTOR (PP-mTOR
Ser2481) during mitosis and cytokinesis in human cancer cells. PP-mTOR
Ser2481 exhibited a punctate nuclear distribution in interphase cancer cells, with the number of PP-mTOR
Ser2481 nuclear speckles positively relating with the proliferative capacity of cancer cells. PP-mTOR
Ser2481 expression dynamically rearranged within the cytoplasm in a close association near and between separating chromosomes during early stages of mitosis. Towards the end of anaphase and in telophase, PP-mTOR
Ser2481 drastically focused on the midzone and ultimately in the centre of the midbody at the presumptive cleavage furrow. In cells at cytokinesis, PP-mTOR
Ser2481 appeared as a doublet facing each other at the apical ends of two daughter cells. Three-dimensional analysis confirmed that PP-mTOR
Ser2481 positioned at a ring structure wrapped round by microtubule bundles to connect daughter cells. These results reveal for the first time that PP-mTOR
Ser2481 may be unexpectedly involved in the terminal stages of cytokinesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19285014</pmid><doi>10.1016/j.bbrc.2009.01.153</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2009-03, Vol.380 (3), p.638-643 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 60 APPLIED LIFE SCIENCES Cancer Carrier Proteins - metabolism CELL CYCLE Cell Division Cell Line, Tumor Cell Nucleus - enzymology Cell Proliferation Chromosomal passenger protein CHROMOSOMES Cytokinesis CYTOPLASM HUMAN POPULATIONS Humans MICROTUBULES MITOSIS mTOR NEOPLASMS Neoplasms - metabolism Neoplasms - pathology Phosphorylation Phosphotransferases (Alcohol Group Acceptor) - metabolism PROTEINS SERINE Serine - metabolism TOR Serine-Threonine Kinases |
| title | The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells |
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