Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis
Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2008-12, Vol.377 (1), p.210-214 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 1 |
| container_start_page | 210 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 377 |
| creator | Xiang, Bin Zhang, Yan Li, Yu-Ming Gao, Yan Gan, Ye-Hua Wu, Li-Ling Yu, Guang-Yan |
| description | Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine reduces the injury has not been fully evaluated. In this study, we investigate the ability of phenylephrine to inhibit apoptosis in autotransplanted rabbit SMG. We observed that apoptosis occurred in the early phase of SMG transplantation and that phenylephrine treatment protected transplanted SMG from apoptosis. Furthermore, we found that phenylephrine could significantly upregulate the expression of Bcl-2, downregulate the expression of Bax, and inhibit the activation of both caspase-3 and p38 mitogen-activated protein kinase in autotransplanted SMG. Therefore, the cytoprotective effects of phenylephrine on autotransplanted SMG may be a novel clinical strategy for autotransplanted SMG protection during the early postoperative stage of transplantation. |
| doi_str_mv | 10.1016/j.bbrc.2008.09.120 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_21217127</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X08018809</els_id><sourcerecordid>69753042</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-230278c6bdfebbcd44bd1bbb45eac89599bc348f3b66e9ef77a17bb1ea1371443</originalsourceid><addsrcrecordid>eNp9kE2LFDEQhoMo7rj6BzxIg-Ct26p0prsDXmRZXWFBBQVvIR_VToaeTpukhf33ppkBb0sdcqinXt48jL1GaBCwe39sjIm24QBDA7JBDk_YDkFCzRHEU7YDgK7mEn9dsRcpHQEQRSefsyscBgFlduz7twPNDxMth-hnqpYYMtmcKr3mkKOe0zLpOZOrojbG5yqt5qRn58066Vj9LktXjTGcKr2EJYfk00v2bNRToleX95r9_HT74-auvv_6-cvNx_vatgPPNW-B94PtjBvJGOuEMA6NMWJP2g5yL6WxrRjG1nQdSRr7XmNvDJLGtkch2mv29pwbUvYqWV-KH2yY59JfceTYI-8L9e5MlZ_9WSlldfLJ0lSKU1iT6mS_b0HwAvIzaGNIKdKoluhPOj4oBLXpVke16VabbgVSFd3l6M0lvWgh9__k4rcAH84AFRN_PcWtKM2WnI9bTxf8Y_n_AAHJkt8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69753042</pqid></control><display><type>article</type><title>Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Collection</source><creator>Xiang, Bin ; Zhang, Yan ; Li, Yu-Ming ; Gao, Yan ; Gan, Ye-Hua ; Wu, Li-Ling ; Yu, Guang-Yan</creator><creatorcontrib>Xiang, Bin ; Zhang, Yan ; Li, Yu-Ming ; Gao, Yan ; Gan, Ye-Hua ; Wu, Li-Ling ; Yu, Guang-Yan</creatorcontrib><description>Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine reduces the injury has not been fully evaluated. In this study, we investigate the ability of phenylephrine to inhibit apoptosis in autotransplanted rabbit SMG. We observed that apoptosis occurred in the early phase of SMG transplantation and that phenylephrine treatment protected transplanted SMG from apoptosis. Furthermore, we found that phenylephrine could significantly upregulate the expression of Bcl-2, downregulate the expression of Bax, and inhibit the activation of both caspase-3 and p38 mitogen-activated protein kinase in autotransplanted SMG. Therefore, the cytoprotective effects of phenylephrine on autotransplanted SMG may be a novel clinical strategy for autotransplanted SMG protection during the early postoperative stage of transplantation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2008.09.120</identifier><identifier>PMID: 18840404</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Adrenergic alpha-Agonists - pharmacology ; Animals ; APOPTOSIS ; Apoptosis - drug effects ; bcl-2-Associated X Protein - metabolism ; Bcl-2/Bax ; Caspase Inhibitors ; Caspase-3 ; CELL PROLIFERATION ; Cytoprotection ; Down-Regulation ; GLANDS ; p38 mitogen-activated protein kinase ; p38 Mitogen-Activated Protein Kinases - metabolism ; Phenylephrine ; Phenylephrine - pharmacology ; Phosphorylation - drug effects ; PROTEINS ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; RABBITS ; Submandibular Gland - drug effects ; Submandibular Gland - transplantation ; Submandibular Gland - ultrastructure ; Transplantation, Autologous ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2008-12, Vol.377 (1), p.210-214</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-230278c6bdfebbcd44bd1bbb45eac89599bc348f3b66e9ef77a17bb1ea1371443</citedby><cites>FETCH-LOGICAL-c382t-230278c6bdfebbcd44bd1bbb45eac89599bc348f3b66e9ef77a17bb1ea1371443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X08018809$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18840404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/21217127$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiang, Bin</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Li, Yu-Ming</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Gan, Ye-Hua</creatorcontrib><creatorcontrib>Wu, Li-Ling</creatorcontrib><creatorcontrib>Yu, Guang-Yan</creatorcontrib><title>Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine reduces the injury has not been fully evaluated. In this study, we investigate the ability of phenylephrine to inhibit apoptosis in autotransplanted rabbit SMG. We observed that apoptosis occurred in the early phase of SMG transplantation and that phenylephrine treatment protected transplanted SMG from apoptosis. Furthermore, we found that phenylephrine could significantly upregulate the expression of Bcl-2, downregulate the expression of Bax, and inhibit the activation of both caspase-3 and p38 mitogen-activated protein kinase in autotransplanted SMG. Therefore, the cytoprotective effects of phenylephrine on autotransplanted SMG may be a novel clinical strategy for autotransplanted SMG protection during the early postoperative stage of transplantation.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Adrenergic alpha-Agonists - pharmacology</subject><subject>Animals</subject><subject>APOPTOSIS</subject><subject>Apoptosis - drug effects</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Bcl-2/Bax</subject><subject>Caspase Inhibitors</subject><subject>Caspase-3</subject><subject>CELL PROLIFERATION</subject><subject>Cytoprotection</subject><subject>Down-Regulation</subject><subject>GLANDS</subject><subject>p38 mitogen-activated protein kinase</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Phenylephrine</subject><subject>Phenylephrine - pharmacology</subject><subject>Phosphorylation - drug effects</subject><subject>PROTEINS</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>RABBITS</subject><subject>Submandibular Gland - drug effects</subject><subject>Submandibular Gland - transplantation</subject><subject>Submandibular Gland - ultrastructure</subject><subject>Transplantation, Autologous</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo7rj6BzxIg-Ct26p0prsDXmRZXWFBBQVvIR_VToaeTpukhf33ppkBb0sdcqinXt48jL1GaBCwe39sjIm24QBDA7JBDk_YDkFCzRHEU7YDgK7mEn9dsRcpHQEQRSefsyscBgFlduz7twPNDxMth-hnqpYYMtmcKr3mkKOe0zLpOZOrojbG5yqt5qRn58066Vj9LktXjTGcKr2EJYfk00v2bNRToleX95r9_HT74-auvv_6-cvNx_vatgPPNW-B94PtjBvJGOuEMA6NMWJP2g5yL6WxrRjG1nQdSRr7XmNvDJLGtkch2mv29pwbUvYqWV-KH2yY59JfceTYI-8L9e5MlZ_9WSlldfLJ0lSKU1iT6mS_b0HwAvIzaGNIKdKoluhPOj4oBLXpVke16VabbgVSFd3l6M0lvWgh9__k4rcAH84AFRN_PcWtKM2WnI9bTxf8Y_n_AAHJkt8</recordid><startdate>20081205</startdate><enddate>20081205</enddate><creator>Xiang, Bin</creator><creator>Zhang, Yan</creator><creator>Li, Yu-Ming</creator><creator>Gao, Yan</creator><creator>Gan, Ye-Hua</creator><creator>Wu, Li-Ling</creator><creator>Yu, Guang-Yan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20081205</creationdate><title>Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis</title><author>Xiang, Bin ; Zhang, Yan ; Li, Yu-Ming ; Gao, Yan ; Gan, Ye-Hua ; Wu, Li-Ling ; Yu, Guang-Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-230278c6bdfebbcd44bd1bbb45eac89599bc348f3b66e9ef77a17bb1ea1371443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Adrenergic alpha-Agonists - pharmacology</topic><topic>Animals</topic><topic>APOPTOSIS</topic><topic>Apoptosis - drug effects</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Bcl-2/Bax</topic><topic>Caspase Inhibitors</topic><topic>Caspase-3</topic><topic>CELL PROLIFERATION</topic><topic>Cytoprotection</topic><topic>Down-Regulation</topic><topic>GLANDS</topic><topic>p38 mitogen-activated protein kinase</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Phenylephrine</topic><topic>Phenylephrine - pharmacology</topic><topic>Phosphorylation - drug effects</topic><topic>PROTEINS</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>RABBITS</topic><topic>Submandibular Gland - drug effects</topic><topic>Submandibular Gland - transplantation</topic><topic>Submandibular Gland - ultrastructure</topic><topic>Transplantation, Autologous</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiang, Bin</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Li, Yu-Ming</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Gan, Ye-Hua</creatorcontrib><creatorcontrib>Wu, Li-Ling</creatorcontrib><creatorcontrib>Yu, Guang-Yan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiang, Bin</au><au>Zhang, Yan</au><au>Li, Yu-Ming</au><au>Gao, Yan</au><au>Gan, Ye-Hua</au><au>Wu, Li-Ling</au><au>Yu, Guang-Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-12-05</date><risdate>2008</risdate><volume>377</volume><issue>1</issue><spage>210</spage><epage>214</epage><pages>210-214</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine reduces the injury has not been fully evaluated. In this study, we investigate the ability of phenylephrine to inhibit apoptosis in autotransplanted rabbit SMG. We observed that apoptosis occurred in the early phase of SMG transplantation and that phenylephrine treatment protected transplanted SMG from apoptosis. Furthermore, we found that phenylephrine could significantly upregulate the expression of Bcl-2, downregulate the expression of Bax, and inhibit the activation of both caspase-3 and p38 mitogen-activated protein kinase in autotransplanted SMG. Therefore, the cytoprotective effects of phenylephrine on autotransplanted SMG may be a novel clinical strategy for autotransplanted SMG protection during the early postoperative stage of transplantation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18840404</pmid><doi>10.1016/j.bbrc.2008.09.120</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2008-12, Vol.377 (1), p.210-214 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_osti_scitechconnect_21217127 |
| source | MEDLINE; Elsevier ScienceDirect Journals Collection |
| subjects | 60 APPLIED LIFE SCIENCES Adrenergic alpha-Agonists - pharmacology Animals APOPTOSIS Apoptosis - drug effects bcl-2-Associated X Protein - metabolism Bcl-2/Bax Caspase Inhibitors Caspase-3 CELL PROLIFERATION Cytoprotection Down-Regulation GLANDS p38 mitogen-activated protein kinase p38 Mitogen-Activated Protein Kinases - metabolism Phenylephrine Phenylephrine - pharmacology Phosphorylation - drug effects PROTEINS Proto-Oncogene Proteins c-bcl-2 - metabolism RABBITS Submandibular Gland - drug effects Submandibular Gland - transplantation Submandibular Gland - ultrastructure Transplantation, Autologous Up-Regulation |
| title | Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T15%3A41%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phenylephrine%20protects%20autotransplanted%20rabbit%20submandibular%20gland%20from%20apoptosis&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Xiang,%20Bin&rft.date=2008-12-05&rft.volume=377&rft.issue=1&rft.spage=210&rft.epage=214&rft.pages=210-214&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2008.09.120&rft_dat=%3Cproquest_osti_%3E69753042%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69753042&rft_id=info:pmid/18840404&rft_els_id=S0006291X08018809&rfr_iscdi=true |