Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells
For long-term culture, murine adipose-derived stromal cells (mADSCs) at latter passages demonstrated a marked decline in proliferative activity, exhibited senescent morphology and reduced differentiation potentials, particularly osteogenesis. To extend the lifespan of mADSCs, two culture conditions...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-08, Vol.360 (1), p.1-6 |
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| creator | Chen, Po-Yang Huang, Lynn L.H. Hsieh, Hsyue-Jen |
| description | For long-term culture, murine adipose-derived stromal cells (mADSCs) at latter passages demonstrated a marked decline in proliferative activity, exhibited senescent morphology and reduced differentiation potentials, particularly osteogenesis. To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. Our results suggested that HA can be useful for preserving the proliferation and differentiation potentials of long-term cultured mADSCs. |
| doi_str_mv | 10.1016/j.bbrc.2007.04.211 |
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To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. 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To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. Our results suggested that HA can be useful for preserving the proliferation and differentiation potentials of long-term cultured mADSCs.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - physiology</subject><subject>Adipose-derived stromal cell</subject><subject>Animals</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation - drug effects</subject><subject>CELL PROLIFERATION</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>CULTURE MEDIA</subject><subject>Differentiation</subject><subject>Dose-Response Relationship, Drug</subject><subject>GENE REGULATION</subject><subject>GROWTH</subject><subject>Hyaluronan</subject><subject>Hyaluronic Acid - administration & dosage</subject><subject>Male</subject><subject>Mice</subject><subject>MORPHOLOGY</subject><subject>Proliferation</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - physiology</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - physiology</subject><subject>Tissue Engineering - methods</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-L1TAUxYMoznP0C7iQgOCu9aZ_0gbcyDA6woAbBXchTW6cPNrkmaQPZudHN7UP3LkK5_K7h3NzCHnNoGbA-PtjPU1R1w3AUENXN4w9IQcGAqqGQfeUHACAV41gP67Ii5SOAIx1XDwnV2zoR97x_kB-3z2qeY3BK09PERPGMyaaH7CoMDuLUWUXPFXeUONs0eiz22enkP-KOdFg6Rz8zypjXKhe57xGNHRZo_NIlXGnkLAyGN25jFOOYVEz1TjP6SV5ZosDvrq81-T7p9tvN3fV_dfPX24-3le6E02uVK-shcEgE3wc-8HysYV2gE503IzGirZXU2NaNpYrlTB2YjiA7kemNROWt9fk7e4bUnYyaZdRP-jgPeosGxCCdeNYqHc7Va7_tWLKcnFpy6k8hjXJAfjAoN_AZgd1DClFtPIU3aLio2Qgt3bkUW7tyK0dCZ0s7ZSlNxf3dVrQ_Fu51FGADzuA5SfODuMWFL1G4-KW0wT3P_8_8i6jdg</recordid><startdate>20070817</startdate><enddate>20070817</enddate><creator>Chen, Po-Yang</creator><creator>Huang, Lynn L.H.</creator><creator>Hsieh, Hsyue-Jen</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20070817</creationdate><title>Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells</title><author>Chen, Po-Yang ; Huang, Lynn L.H. ; Hsieh, Hsyue-Jen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-a5aff07de1968857f68303704946d8df935ab2d318001a9dfb1e70c581cc19f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Adipocytes - cytology</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - physiology</topic><topic>Adipose-derived stromal cell</topic><topic>Animals</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation - drug effects</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>CULTURE MEDIA</topic><topic>Differentiation</topic><topic>Dose-Response Relationship, Drug</topic><topic>GENE REGULATION</topic><topic>GROWTH</topic><topic>Hyaluronan</topic><topic>Hyaluronic Acid - administration & dosage</topic><topic>Male</topic><topic>Mice</topic><topic>MORPHOLOGY</topic><topic>Proliferation</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - physiology</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - physiology</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Po-Yang</creatorcontrib><creatorcontrib>Huang, Lynn L.H.</creatorcontrib><creatorcontrib>Hsieh, Hsyue-Jen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Po-Yang</au><au>Huang, Lynn L.H.</au><au>Hsieh, Hsyue-Jen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-08-17</date><risdate>2007</risdate><volume>360</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>For long-term culture, murine adipose-derived stromal cells (mADSCs) at latter passages demonstrated a marked decline in proliferative activity, exhibited senescent morphology and reduced differentiation potentials, particularly osteogenesis. To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. Our results suggested that HA can be useful for preserving the proliferation and differentiation potentials of long-term cultured mADSCs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17586465</pmid><doi>10.1016/j.bbrc.2007.04.211</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Adipocytes - cytology Adipocytes - drug effects Adipocytes - physiology Adipose-derived stromal cell Animals Cell Culture Techniques - methods Cell Differentiation - drug effects CELL PROLIFERATION Cell Proliferation - drug effects Cells, Cultured CULTURE MEDIA Differentiation Dose-Response Relationship, Drug GENE REGULATION GROWTH Hyaluronan Hyaluronic Acid - administration & dosage Male Mice MORPHOLOGY Proliferation Stem Cells - cytology Stem Cells - drug effects Stem Cells - physiology Stromal Cells - cytology Stromal Cells - drug effects Stromal Cells - physiology Tissue Engineering - methods |
| title | Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells |
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