Differential expression of TRAIL and its receptors relative to calcification in AAA
Abdominal aortic aneurysm (AAA) is commonly associated with atherosclerosis. Human AAA tissue displays cells undergoing all stages of apoptosis. Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumour cells but not in normal cells. It has death receptors an...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-06, Vol.358 (1), p.18-23 |
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| creator | Liu, Xun Winrow, Vivienne R. Horrocks, Michael Stevens, Cliff R. |
| description | Abdominal aortic aneurysm (AAA) is commonly associated with atherosclerosis. Human AAA tissue displays cells undergoing all stages of apoptosis. Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumour cells but not in normal cells. It has death receptors and decoy receptors. An inhibitor of TRAIL, osteoprotegerin (OPG), is involved in osteogenesis and vascular calcification. We investigated TRAIL and its receptors in AAA compared within normal aorta (NA).
Both qualitative and quantitative analyses of calcification in AAA walls were determined using Von Kossa staining and pre-operation computer tomography (CT) scans. There was a significant difference in calcification level at different locations in the AAA wall (
p <
0.05). Apoptosis was confirmed in AAA by TUNEL assay. A significant difference in TRAIL and its receptor expression was observed between normal aortae and AAA (
p
<
0.05). Significant differences were also observed between tissues displaying different extents of calcification for TRAIL mRNA (
p
<
0.05) by RT-PCR examination and OPG protein (
p
<
0.01) by protein blotting examination.
We propose that this pattern of expression of TRAIL and its receptors may contribute to AAA formation and calcification in the AAA wall. |
| doi_str_mv | 10.1016/j.bbrc.2007.03.209 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_20991393</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X07007000</els_id><sourcerecordid>70508248</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-81f94565509979c1fe1ed246316e79101c07577c1dbc6cb6e687b66b67bf12743</originalsourceid><addsrcrecordid>eNqFkU-LFDEQxYMo7rj6BTxIQPDWY1W6O5mAl2bXPwsDC-sK3kJ3uoIZejpjkll2v71pZsCbnqoIv_dIvcfYW4Q1AsqPu_UwRLsWAGoNdZn6GVshaKgEQvOcrQBAVkLjzwv2KqUdAGIj9Ut2garRIAWu2Pdr7xxFmrPvJ06Ph0gp-TDz4Pj9XXez5f08cp8Tj2TpkENctqnP_oF4Dtz2k_XO2_JQRH7mXde9Zi9cPyV6c56X7MeXz_dX36rt7debq25b2QbrXG3Q6aaVbQtaK23REdIoGlmjJKXLhRZUq5TFcbDSDpLkRg1SDlINDoVq6kv2_uQbUvYmWZ_J_rJhnslmU9LQWOu6UB9O1CGG30dK2ex9sjRN_UzhmIyCFjai2fwXFFCiBLWA4gTaGFKK5Mwh-n0fnwyCWZoxO7M0Y5ZmDNTLX4ro3dn9OOxp_Cs5V1GATyeASmQPnuJyEc2WRh-Xg8bg_-X_B2RGnM8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20291078</pqid></control><display><type>article</type><title>Differential expression of TRAIL and its receptors relative to calcification in AAA</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Liu, Xun ; Winrow, Vivienne R. ; Horrocks, Michael ; Stevens, Cliff R.</creator><creatorcontrib>Liu, Xun ; Winrow, Vivienne R. ; Horrocks, Michael ; Stevens, Cliff R.</creatorcontrib><description>Abdominal aortic aneurysm (AAA) is commonly associated with atherosclerosis. Human AAA tissue displays cells undergoing all stages of apoptosis. Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumour cells but not in normal cells. It has death receptors and decoy receptors. An inhibitor of TRAIL, osteoprotegerin (OPG), is involved in osteogenesis and vascular calcification. We investigated TRAIL and its receptors in AAA compared within normal aorta (NA).
Both qualitative and quantitative analyses of calcification in AAA walls were determined using Von Kossa staining and pre-operation computer tomography (CT) scans. There was a significant difference in calcification level at different locations in the AAA wall (
p <
0.05). Apoptosis was confirmed in AAA by TUNEL assay. A significant difference in TRAIL and its receptor expression was observed between normal aortae and AAA (
p
<
0.05). Significant differences were also observed between tissues displaying different extents of calcification for TRAIL mRNA (
p
<
0.05) by RT-PCR examination and OPG protein (
p
<
0.01) by protein blotting examination.
We propose that this pattern of expression of TRAIL and its receptors may contribute to AAA formation and calcification in the AAA wall.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.03.209</identifier><identifier>PMID: 17490621</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Abdominal aortic aneurysm ; Aged ; Aged, 80 and over ; AORTA ; Aorta, Abdominal - diagnostic imaging ; Aorta, Abdominal - metabolism ; Aorta, Abdominal - pathology ; Aortic Aneurysm, Abdominal - diagnostic imaging ; Aortic Aneurysm, Abdominal - metabolism ; Aortic Aneurysm, Abdominal - pathology ; APOPTOSIS ; ARTERIOSCLEROSIS ; Calcinosis - diagnostic imaging ; Calcinosis - metabolism ; Calcinosis - pathology ; COMPUTERIZED TOMOGRAPHY ; Female ; Humans ; LIGANDS ; Male ; Middle Aged ; NECROSIS ; Osteoprotegerin ; POLYMERASE CHAIN REACTION ; RECEPTORS ; Receptors, TNF-Related Apoptosis-Inducing Ligand - metabolism ; TNF-related apoptosis-inducing ligand (TRAIL) ; TNF-Related Apoptosis-Inducing Ligand - metabolism ; Tomography, X-Ray Computed ; TRAIL receptors ; Vascular calcification</subject><ispartof>Biochemical and biophysical research communications, 2007-06, Vol.358 (1), p.18-23</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-81f94565509979c1fe1ed246316e79101c07577c1dbc6cb6e687b66b67bf12743</citedby><cites>FETCH-LOGICAL-c413t-81f94565509979c1fe1ed246316e79101c07577c1dbc6cb6e687b66b67bf12743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2007.03.209$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17490621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20991393$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xun</creatorcontrib><creatorcontrib>Winrow, Vivienne R.</creatorcontrib><creatorcontrib>Horrocks, Michael</creatorcontrib><creatorcontrib>Stevens, Cliff R.</creatorcontrib><title>Differential expression of TRAIL and its receptors relative to calcification in AAA</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Abdominal aortic aneurysm (AAA) is commonly associated with atherosclerosis. Human AAA tissue displays cells undergoing all stages of apoptosis. Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumour cells but not in normal cells. It has death receptors and decoy receptors. An inhibitor of TRAIL, osteoprotegerin (OPG), is involved in osteogenesis and vascular calcification. We investigated TRAIL and its receptors in AAA compared within normal aorta (NA).
Both qualitative and quantitative analyses of calcification in AAA walls were determined using Von Kossa staining and pre-operation computer tomography (CT) scans. There was a significant difference in calcification level at different locations in the AAA wall (
p <
0.05). Apoptosis was confirmed in AAA by TUNEL assay. A significant difference in TRAIL and its receptor expression was observed between normal aortae and AAA (
p
<
0.05). Significant differences were also observed between tissues displaying different extents of calcification for TRAIL mRNA (
p
<
0.05) by RT-PCR examination and OPG protein (
p
<
0.01) by protein blotting examination.
We propose that this pattern of expression of TRAIL and its receptors may contribute to AAA formation and calcification in the AAA wall.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Abdominal aortic aneurysm</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>AORTA</subject><subject>Aorta, Abdominal - diagnostic imaging</subject><subject>Aorta, Abdominal - metabolism</subject><subject>Aorta, Abdominal - pathology</subject><subject>Aortic Aneurysm, Abdominal - diagnostic imaging</subject><subject>Aortic Aneurysm, Abdominal - metabolism</subject><subject>Aortic Aneurysm, Abdominal - pathology</subject><subject>APOPTOSIS</subject><subject>ARTERIOSCLEROSIS</subject><subject>Calcinosis - diagnostic imaging</subject><subject>Calcinosis - metabolism</subject><subject>Calcinosis - pathology</subject><subject>COMPUTERIZED TOMOGRAPHY</subject><subject>Female</subject><subject>Humans</subject><subject>LIGANDS</subject><subject>Male</subject><subject>Middle Aged</subject><subject>NECROSIS</subject><subject>Osteoprotegerin</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>RECEPTORS</subject><subject>Receptors, TNF-Related Apoptosis-Inducing Ligand - metabolism</subject><subject>TNF-related apoptosis-inducing ligand (TRAIL)</subject><subject>TNF-Related Apoptosis-Inducing Ligand - metabolism</subject><subject>Tomography, X-Ray Computed</subject><subject>TRAIL receptors</subject><subject>Vascular calcification</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo7rj6BTxIQPDWY1W6O5mAl2bXPwsDC-sK3kJ3uoIZejpjkll2v71pZsCbnqoIv_dIvcfYW4Q1AsqPu_UwRLsWAGoNdZn6GVshaKgEQvOcrQBAVkLjzwv2KqUdAGIj9Ut2garRIAWu2Pdr7xxFmrPvJ06Ph0gp-TDz4Pj9XXez5f08cp8Tj2TpkENctqnP_oF4Dtz2k_XO2_JQRH7mXde9Zi9cPyV6c56X7MeXz_dX36rt7debq25b2QbrXG3Q6aaVbQtaK23REdIoGlmjJKXLhRZUq5TFcbDSDpLkRg1SDlINDoVq6kv2_uQbUvYmWZ_J_rJhnslmU9LQWOu6UB9O1CGG30dK2ex9sjRN_UzhmIyCFjai2fwXFFCiBLWA4gTaGFKK5Mwh-n0fnwyCWZoxO7M0Y5ZmDNTLX4ro3dn9OOxp_Cs5V1GATyeASmQPnuJyEc2WRh-Xg8bg_-X_B2RGnM8</recordid><startdate>20070622</startdate><enddate>20070622</enddate><creator>Liu, Xun</creator><creator>Winrow, Vivienne R.</creator><creator>Horrocks, Michael</creator><creator>Stevens, Cliff R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20070622</creationdate><title>Differential expression of TRAIL and its receptors relative to calcification in AAA</title><author>Liu, Xun ; Winrow, Vivienne R. ; Horrocks, Michael ; Stevens, Cliff R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-81f94565509979c1fe1ed246316e79101c07577c1dbc6cb6e687b66b67bf12743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Abdominal aortic aneurysm</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>AORTA</topic><topic>Aorta, Abdominal - diagnostic imaging</topic><topic>Aorta, Abdominal - metabolism</topic><topic>Aorta, Abdominal - pathology</topic><topic>Aortic Aneurysm, Abdominal - diagnostic imaging</topic><topic>Aortic Aneurysm, Abdominal - metabolism</topic><topic>Aortic Aneurysm, Abdominal - pathology</topic><topic>APOPTOSIS</topic><topic>ARTERIOSCLEROSIS</topic><topic>Calcinosis - diagnostic imaging</topic><topic>Calcinosis - metabolism</topic><topic>Calcinosis - pathology</topic><topic>COMPUTERIZED TOMOGRAPHY</topic><topic>Female</topic><topic>Humans</topic><topic>LIGANDS</topic><topic>Male</topic><topic>Middle Aged</topic><topic>NECROSIS</topic><topic>Osteoprotegerin</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>RECEPTORS</topic><topic>Receptors, TNF-Related Apoptosis-Inducing Ligand - metabolism</topic><topic>TNF-related apoptosis-inducing ligand (TRAIL)</topic><topic>TNF-Related Apoptosis-Inducing Ligand - metabolism</topic><topic>Tomography, X-Ray Computed</topic><topic>TRAIL receptors</topic><topic>Vascular calcification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xun</creatorcontrib><creatorcontrib>Winrow, Vivienne R.</creatorcontrib><creatorcontrib>Horrocks, Michael</creatorcontrib><creatorcontrib>Stevens, Cliff R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xun</au><au>Winrow, Vivienne R.</au><au>Horrocks, Michael</au><au>Stevens, Cliff R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of TRAIL and its receptors relative to calcification in AAA</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-06-22</date><risdate>2007</risdate><volume>358</volume><issue>1</issue><spage>18</spage><epage>23</epage><pages>18-23</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Abdominal aortic aneurysm (AAA) is commonly associated with atherosclerosis. Human AAA tissue displays cells undergoing all stages of apoptosis. Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumour cells but not in normal cells. It has death receptors and decoy receptors. An inhibitor of TRAIL, osteoprotegerin (OPG), is involved in osteogenesis and vascular calcification. We investigated TRAIL and its receptors in AAA compared within normal aorta (NA).
Both qualitative and quantitative analyses of calcification in AAA walls were determined using Von Kossa staining and pre-operation computer tomography (CT) scans. There was a significant difference in calcification level at different locations in the AAA wall (
p <
0.05). Apoptosis was confirmed in AAA by TUNEL assay. A significant difference in TRAIL and its receptor expression was observed between normal aortae and AAA (
p
<
0.05). Significant differences were also observed between tissues displaying different extents of calcification for TRAIL mRNA (
p
<
0.05) by RT-PCR examination and OPG protein (
p
<
0.01) by protein blotting examination.
We propose that this pattern of expression of TRAIL and its receptors may contribute to AAA formation and calcification in the AAA wall.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17490621</pmid><doi>10.1016/j.bbrc.2007.03.209</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2007-06, Vol.358 (1), p.18-23 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 60 APPLIED LIFE SCIENCES Abdominal aortic aneurysm Aged Aged, 80 and over AORTA Aorta, Abdominal - diagnostic imaging Aorta, Abdominal - metabolism Aorta, Abdominal - pathology Aortic Aneurysm, Abdominal - diagnostic imaging Aortic Aneurysm, Abdominal - metabolism Aortic Aneurysm, Abdominal - pathology APOPTOSIS ARTERIOSCLEROSIS Calcinosis - diagnostic imaging Calcinosis - metabolism Calcinosis - pathology COMPUTERIZED TOMOGRAPHY Female Humans LIGANDS Male Middle Aged NECROSIS Osteoprotegerin POLYMERASE CHAIN REACTION RECEPTORS Receptors, TNF-Related Apoptosis-Inducing Ligand - metabolism TNF-related apoptosis-inducing ligand (TRAIL) TNF-Related Apoptosis-Inducing Ligand - metabolism Tomography, X-Ray Computed TRAIL receptors Vascular calcification |
| title | Differential expression of TRAIL and its receptors relative to calcification in AAA |
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