siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis
Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while pati...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2007-06, Vol.357 (2), p.353-359 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 359 |
|---|---|
| container_issue | 2 |
| container_start_page | 353 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 357 |
| creator | Wada, Makoto Kawahito, Yutaka Kimura, Shinya Kohno, Masataka Ishino, Hidetaka Kimura, Mizuho Omoto, Atsushi Yamamoto, Aihiro Hamaguchi, Masahide Tsubouchi, Yasunori Tokunaga, Daisaku Hojo, Tatsuya Ashihara, Eishi Maekawa, Taira Yoshikawa, Toshikazu |
| description | Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1β in RA synoviocytes. IL-1β also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1β and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA. |
| doi_str_mv | 10.1016/j.bbrc.2007.03.190 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_20991361</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X07005979</els_id><sourcerecordid>70415400</sourcerecordid><originalsourceid>FETCH-LOGICAL-c479t-7c5acf72dc48ebc94757f3e0811d6fcc236db16de3c03996473e03d537322cc23</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo7rj6BzxIg-Ctx6ok05mAl2XxCwdXRMFb6K5U72SY6YxJemH-vd3MgDc91eF96oWqR4iXCEsEbN7ull2XaCkBzBLUEi08EgsEC7VE0I_FAgCaWlr8dSWe5bwDQNSNfSqu0GilUeuFuMvh-9ebqrTpnksY7qtvmy81VmHwI3Gu2mM8lphDrmJf5dMQH0KkU5mSMFRpy-OhLTH4qk1lm0IJ-bl40rf7zC8u81r8_PD-x-2nenP38fPtzaYmbWypDa1a6o30pNfckdVmZXrFsEb0TU8kVeM7bDwrAmVto80UKr9SRkk5x9fi9bk35hJcplCYthSHgak4CdaianCi3pypY4q_R87FHUIm3u_bgeOYnQGNKw3wX1DCWoNaz43yDFKKOSfu3TGFQ5tODsHNVtzOzVbcbMWBcpOVaenVpX3sDuz_rlw0TMC7M8DTyx4Cp_kiHoh9SPNBPoZ_9f8BwY6c8g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20840381</pqid></control><display><type>article</type><title>siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Wada, Makoto ; Kawahito, Yutaka ; Kimura, Shinya ; Kohno, Masataka ; Ishino, Hidetaka ; Kimura, Mizuho ; Omoto, Atsushi ; Yamamoto, Aihiro ; Hamaguchi, Masahide ; Tsubouchi, Yasunori ; Tokunaga, Daisaku ; Hojo, Tatsuya ; Ashihara, Eishi ; Maekawa, Taira ; Yoshikawa, Toshikazu</creator><creatorcontrib>Wada, Makoto ; Kawahito, Yutaka ; Kimura, Shinya ; Kohno, Masataka ; Ishino, Hidetaka ; Kimura, Mizuho ; Omoto, Atsushi ; Yamamoto, Aihiro ; Hamaguchi, Masahide ; Tsubouchi, Yasunori ; Tokunaga, Daisaku ; Hojo, Tatsuya ; Ashihara, Eishi ; Maekawa, Taira ; Yoshikawa, Toshikazu</creatorcontrib><description>Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1β in RA synoviocytes. IL-1β also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1β and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.03.190</identifier><identifier>PMID: 17434144</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>APOPTOSIS ; Apoptosis - genetics ; Arthritis, Rheumatoid - pathology ; Arthritis, Rheumatoid - physiopathology ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; CELL PROLIFERATION ; Cells, Cultured ; Gene Silencing ; Gene Targeting - methods ; Humans ; INJURIES ; MITOSIS ; PATIENTS ; PLK-1 ; Polo-Like Kinase 1 ; Protein Serine-Threonine Kinases - genetics ; Protein Serine-Threonine Kinases - metabolism ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; RADIOLOGY AND NUCLEAR MEDICINE ; RHEUMATIC DISEASES ; Rheumatoid arthritis ; RNA, Small Interfering - genetics ; siRNA ; SKELETON ; Synovial Fluid - cytology ; Synovial Fluid - metabolism ; Synoviocyte</subject><ispartof>Biochemical and biophysical research communications, 2007-06, Vol.357 (2), p.353-359</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-7c5acf72dc48ebc94757f3e0811d6fcc236db16de3c03996473e03d537322cc23</citedby><cites>FETCH-LOGICAL-c479t-7c5acf72dc48ebc94757f3e0811d6fcc236db16de3c03996473e03d537322cc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2007.03.190$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17434144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20991361$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wada, Makoto</creatorcontrib><creatorcontrib>Kawahito, Yutaka</creatorcontrib><creatorcontrib>Kimura, Shinya</creatorcontrib><creatorcontrib>Kohno, Masataka</creatorcontrib><creatorcontrib>Ishino, Hidetaka</creatorcontrib><creatorcontrib>Kimura, Mizuho</creatorcontrib><creatorcontrib>Omoto, Atsushi</creatorcontrib><creatorcontrib>Yamamoto, Aihiro</creatorcontrib><creatorcontrib>Hamaguchi, Masahide</creatorcontrib><creatorcontrib>Tsubouchi, Yasunori</creatorcontrib><creatorcontrib>Tokunaga, Daisaku</creatorcontrib><creatorcontrib>Hojo, Tatsuya</creatorcontrib><creatorcontrib>Ashihara, Eishi</creatorcontrib><creatorcontrib>Maekawa, Taira</creatorcontrib><creatorcontrib>Yoshikawa, Toshikazu</creatorcontrib><title>siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1β in RA synoviocytes. IL-1β also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1β and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA.</description><subject>APOPTOSIS</subject><subject>Apoptosis - genetics</subject><subject>Arthritis, Rheumatoid - pathology</subject><subject>Arthritis, Rheumatoid - physiopathology</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>CELL PROLIFERATION</subject><subject>Cells, Cultured</subject><subject>Gene Silencing</subject><subject>Gene Targeting - methods</subject><subject>Humans</subject><subject>INJURIES</subject><subject>MITOSIS</subject><subject>PATIENTS</subject><subject>PLK-1</subject><subject>Polo-Like Kinase 1</subject><subject>Protein Serine-Threonine Kinases - genetics</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>RADIOLOGY AND NUCLEAR MEDICINE</subject><subject>RHEUMATIC DISEASES</subject><subject>Rheumatoid arthritis</subject><subject>RNA, Small Interfering - genetics</subject><subject>siRNA</subject><subject>SKELETON</subject><subject>Synovial Fluid - cytology</subject><subject>Synovial Fluid - metabolism</subject><subject>Synoviocyte</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rj6BzxIg-Ctx6ok05mAl2XxCwdXRMFb6K5U72SY6YxJemH-vd3MgDc91eF96oWqR4iXCEsEbN7ull2XaCkBzBLUEi08EgsEC7VE0I_FAgCaWlr8dSWe5bwDQNSNfSqu0GilUeuFuMvh-9ebqrTpnksY7qtvmy81VmHwI3Gu2mM8lphDrmJf5dMQH0KkU5mSMFRpy-OhLTH4qk1lm0IJ-bl40rf7zC8u81r8_PD-x-2nenP38fPtzaYmbWypDa1a6o30pNfckdVmZXrFsEb0TU8kVeM7bDwrAmVto80UKr9SRkk5x9fi9bk35hJcplCYthSHgak4CdaianCi3pypY4q_R87FHUIm3u_bgeOYnQGNKw3wX1DCWoNaz43yDFKKOSfu3TGFQ5tODsHNVtzOzVbcbMWBcpOVaenVpX3sDuz_rlw0TMC7M8DTyx4Cp_kiHoh9SPNBPoZ_9f8BwY6c8g</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Wada, Makoto</creator><creator>Kawahito, Yutaka</creator><creator>Kimura, Shinya</creator><creator>Kohno, Masataka</creator><creator>Ishino, Hidetaka</creator><creator>Kimura, Mizuho</creator><creator>Omoto, Atsushi</creator><creator>Yamamoto, Aihiro</creator><creator>Hamaguchi, Masahide</creator><creator>Tsubouchi, Yasunori</creator><creator>Tokunaga, Daisaku</creator><creator>Hojo, Tatsuya</creator><creator>Ashihara, Eishi</creator><creator>Maekawa, Taira</creator><creator>Yoshikawa, Toshikazu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20070601</creationdate><title>siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis</title><author>Wada, Makoto ; Kawahito, Yutaka ; Kimura, Shinya ; Kohno, Masataka ; Ishino, Hidetaka ; Kimura, Mizuho ; Omoto, Atsushi ; Yamamoto, Aihiro ; Hamaguchi, Masahide ; Tsubouchi, Yasunori ; Tokunaga, Daisaku ; Hojo, Tatsuya ; Ashihara, Eishi ; Maekawa, Taira ; Yoshikawa, Toshikazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-7c5acf72dc48ebc94757f3e0811d6fcc236db16de3c03996473e03d537322cc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>APOPTOSIS</topic><topic>Apoptosis - genetics</topic><topic>Arthritis, Rheumatoid - pathology</topic><topic>Arthritis, Rheumatoid - physiopathology</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>CELL PROLIFERATION</topic><topic>Cells, Cultured</topic><topic>Gene Silencing</topic><topic>Gene Targeting - methods</topic><topic>Humans</topic><topic>INJURIES</topic><topic>MITOSIS</topic><topic>PATIENTS</topic><topic>PLK-1</topic><topic>Polo-Like Kinase 1</topic><topic>Protein Serine-Threonine Kinases - genetics</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>RADIOLOGY AND NUCLEAR MEDICINE</topic><topic>RHEUMATIC DISEASES</topic><topic>Rheumatoid arthritis</topic><topic>RNA, Small Interfering - genetics</topic><topic>siRNA</topic><topic>SKELETON</topic><topic>Synovial Fluid - cytology</topic><topic>Synovial Fluid - metabolism</topic><topic>Synoviocyte</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wada, Makoto</creatorcontrib><creatorcontrib>Kawahito, Yutaka</creatorcontrib><creatorcontrib>Kimura, Shinya</creatorcontrib><creatorcontrib>Kohno, Masataka</creatorcontrib><creatorcontrib>Ishino, Hidetaka</creatorcontrib><creatorcontrib>Kimura, Mizuho</creatorcontrib><creatorcontrib>Omoto, Atsushi</creatorcontrib><creatorcontrib>Yamamoto, Aihiro</creatorcontrib><creatorcontrib>Hamaguchi, Masahide</creatorcontrib><creatorcontrib>Tsubouchi, Yasunori</creatorcontrib><creatorcontrib>Tokunaga, Daisaku</creatorcontrib><creatorcontrib>Hojo, Tatsuya</creatorcontrib><creatorcontrib>Ashihara, Eishi</creatorcontrib><creatorcontrib>Maekawa, Taira</creatorcontrib><creatorcontrib>Yoshikawa, Toshikazu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wada, Makoto</au><au>Kawahito, Yutaka</au><au>Kimura, Shinya</au><au>Kohno, Masataka</au><au>Ishino, Hidetaka</au><au>Kimura, Mizuho</au><au>Omoto, Atsushi</au><au>Yamamoto, Aihiro</au><au>Hamaguchi, Masahide</au><au>Tsubouchi, Yasunori</au><au>Tokunaga, Daisaku</au><au>Hojo, Tatsuya</au><au>Ashihara, Eishi</au><au>Maekawa, Taira</au><au>Yoshikawa, Toshikazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>357</volume><issue>2</issue><spage>353</spage><epage>359</epage><pages>353-359</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Polo-like kinase-1 (PLK-1) is a member of the PLK family and participates in the control of cell mitosis. Here, we show that immunoreactive PLK-1 is strongly expressed in synoviocytes and some infiltrative mononuclear cells in synovial tissues from patients with rheumatoid arthritis (RA), while patients with osteoarthritis and injury show little or no expression of PLK-1 in synovial tissues. Western blot analysis shows that PLK is expressed and its expression is enhanced by IL-1β in RA synoviocytes. IL-1β also enhanced the cell growth of RA synoviocytes. Moreover, siRNA targeted against PLK-1 significantly decreases the expression of PLK-1 of RA synoviocytes stimulated by IL-1β and suppresses the proliferation of these synoviocytes through apoptosis. These findings suggest that PLK-1 plays a critical role in the proliferation of RA synoviocytes leading to bone destruction, and siRNA against PLK-1 is potentially useful for the treatment of RA.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17434144</pmid><doi>10.1016/j.bbrc.2007.03.190</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2007-06, Vol.357 (2), p.353-359 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_osti_scitechconnect_20991361 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | APOPTOSIS Apoptosis - genetics Arthritis, Rheumatoid - pathology Arthritis, Rheumatoid - physiopathology Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism CELL PROLIFERATION Cells, Cultured Gene Silencing Gene Targeting - methods Humans INJURIES MITOSIS PATIENTS PLK-1 Polo-Like Kinase 1 Protein Serine-Threonine Kinases - genetics Protein Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism RADIOLOGY AND NUCLEAR MEDICINE RHEUMATIC DISEASES Rheumatoid arthritis RNA, Small Interfering - genetics siRNA SKELETON Synovial Fluid - cytology Synovial Fluid - metabolism Synoviocyte |
| title | siRNA targeting PLK-1 induces apoptosis of synoviocytes in rheumatoid arthritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T22%3A40%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=siRNA%20targeting%20PLK-1%20induces%20apoptosis%20of%20synoviocytes%20in%20rheumatoid%20arthritis&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Wada,%20Makoto&rft.date=2007-06-01&rft.volume=357&rft.issue=2&rft.spage=353&rft.epage=359&rft.pages=353-359&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2007.03.190&rft_dat=%3Cproquest_osti_%3E70415400%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20840381&rft_id=info:pmid/17434144&rft_els_id=S0006291X07005979&rfr_iscdi=true |