Identification of peroxisome-proliferator responsive element in the mouse HSL gene
Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step of lipolysis in adipose tissue. Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidat...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-01, Vol.352 (2), p.526-531 |
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| creator | Yajima, Hiroaki Kobayashi, Yumie Kanaya, Tomoka Horino, Yoko |
| description | Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step of lipolysis in adipose tissue. Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5′-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPARγ) and retinoid X receptor (RXRα) interacted with the region. Binding of the PPARγ/RXRα heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. These results indicate that the HSL gene is transcriptionally regulated by PPARγ/RXRα heterodimer, and suggest that a cis-acting element regulates the HSL gene expression. |
| doi_str_mv | 10.1016/j.bbrc.2006.11.054 |
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Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5′-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPARγ) and retinoid X receptor (RXRα) interacted with the region. Binding of the PPARγ/RXRα heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. 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Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5′-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPARγ) and retinoid X receptor (RXRα) interacted with the region. Binding of the PPARγ/RXRα heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. These results indicate that the HSL gene is transcriptionally regulated by PPARγ/RXRα heterodimer, and suggest that a cis-acting element regulates the HSL gene expression.</description><subject>3T3-L1 adipocyte</subject><subject>3T3-L1 Cells</subject><subject>60 APPLIED LIFE SCIENCES</subject><subject>Adipocytes - enzymology</subject><subject>ADIPOSE TISSUE</subject><subject>Animals</subject><subject>CHROMATIN</subject><subject>Gene Expression Regulation - physiology</subject><subject>GENE REGULATION</subject><subject>GENES</subject><subject>Hormone-sensitive lipase</subject><subject>HORMONES</subject><subject>LIPASES</subject><subject>MICE</subject><subject>MUTANTS</subject><subject>Peroxisome Proliferators - metabolism</subject><subject>Peroxisome-proliferator activated receptor</subject><subject>Peroxisome-proliferator responsive element</subject><subject>PHOSPHORYLATION</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>PROMOTERS</subject><subject>RECEPTORS</subject><subject>Response Elements - genetics</subject><subject>Sterol Esterase - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFrFDEYhoNY7Fr9Ax4kIHibab6ZycwEeilFbWFBaCt4C5nki80yk6xJttR_b4Zd8OYpgTzvy5uHkA_AamDQX-7qaYq6bhjra4Ca8e4V2QATrGqAda_JhpWXqhHw85y8TWnHGEDXizfkHAZou37oN-T-zqDPzjqtsgueBkv3GMOLS2HBah_D7CxGlUOkEdM--OSekeKMS4lR52l-QrqEQ0J6-7Clv9DjO3Jm1Zzw_em8ID--fnm8ua2237_d3VxvK90NIlcTttYOYyuUHXuFqh250R3T1ugBOZi-b8p9aiZhNOcjM-04MKvUNJoWp5a3F-TTsTek7GTSLqN-0sF71Fk2bOSD4KJQn49U-cvvA6YsF5c0zrPyWGZLECNnTTcUsDmCOoaUIlq5j25R8Y8EJlffcidX33L1LQFk8V1CH0_th2lB8y9yElyAqyOAxcSzw7gORa_RuLjuNMH9r_8v03uS2Q</recordid><startdate>20070112</startdate><enddate>20070112</enddate><creator>Yajima, Hiroaki</creator><creator>Kobayashi, Yumie</creator><creator>Kanaya, Tomoka</creator><creator>Horino, Yoko</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope></search><sort><creationdate>20070112</creationdate><title>Identification of peroxisome-proliferator responsive element in the mouse HSL gene</title><author>Yajima, Hiroaki ; Kobayashi, Yumie ; Kanaya, Tomoka ; Horino, Yoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-be3ff7839af86aea385dc40cfdc7e51d662cfdb2b9dc5580d3870faab8d3eb353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>3T3-L1 adipocyte</topic><topic>3T3-L1 Cells</topic><topic>60 APPLIED LIFE SCIENCES</topic><topic>Adipocytes - enzymology</topic><topic>ADIPOSE TISSUE</topic><topic>Animals</topic><topic>CHROMATIN</topic><topic>Gene Expression Regulation - physiology</topic><topic>GENE REGULATION</topic><topic>GENES</topic><topic>Hormone-sensitive lipase</topic><topic>HORMONES</topic><topic>LIPASES</topic><topic>MICE</topic><topic>MUTANTS</topic><topic>Peroxisome Proliferators - metabolism</topic><topic>Peroxisome-proliferator activated receptor</topic><topic>Peroxisome-proliferator responsive element</topic><topic>PHOSPHORYLATION</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>PROMOTERS</topic><topic>RECEPTORS</topic><topic>Response Elements - genetics</topic><topic>Sterol Esterase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yajima, Hiroaki</creatorcontrib><creatorcontrib>Kobayashi, Yumie</creatorcontrib><creatorcontrib>Kanaya, Tomoka</creatorcontrib><creatorcontrib>Horino, Yoko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yajima, Hiroaki</au><au>Kobayashi, Yumie</au><au>Kanaya, Tomoka</au><au>Horino, Yoko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of peroxisome-proliferator responsive element in the mouse HSL gene</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-01-12</date><risdate>2007</risdate><volume>352</volume><issue>2</issue><spage>526</spage><epage>531</epage><pages>526-531</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step of lipolysis in adipose tissue. Several studies suggest that protein phosphorylation regulates the HSL enzymatic activity. On the other hand, the precise mechanism of the transcriptional regulation of the HSL gene remains to be elucidated. Here, we identified a functional peroxisome-proliferator responsive element (PPRE) in the mouse HSL promoter by reporter assay in CV-1 cells using serial deletion and point mutants of the 5′-flanking region. Chromatin immunoprecipitation (ChIP) analysis revealed that both peroxisome-proliferator activated receptor (PPARγ) and retinoid X receptor (RXRα) interacted with the region. Binding of the PPARγ/RXRα heterodimer to the PPRE sequence was also confirmed by electrophoretic mobility shift assay. These results indicate that the HSL gene is transcriptionally regulated by PPARγ/RXRα heterodimer, and suggest that a cis-acting element regulates the HSL gene expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17134676</pmid><doi>10.1016/j.bbrc.2006.11.054</doi><tpages>6</tpages></addata></record> |
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| subjects | 3T3-L1 adipocyte 3T3-L1 Cells 60 APPLIED LIFE SCIENCES Adipocytes - enzymology ADIPOSE TISSUE Animals CHROMATIN Gene Expression Regulation - physiology GENE REGULATION GENES Hormone-sensitive lipase HORMONES LIPASES MICE MUTANTS Peroxisome Proliferators - metabolism Peroxisome-proliferator activated receptor Peroxisome-proliferator responsive element PHOSPHORYLATION Promoter Regions, Genetic - genetics PROMOTERS RECEPTORS Response Elements - genetics Sterol Esterase - genetics |
| title | Identification of peroxisome-proliferator responsive element in the mouse HSL gene |
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