The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells
Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cell...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2006-08, Vol.346 (3), p.919-925 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 925 |
|---|---|
| container_issue | 3 |
| container_start_page | 919 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 346 |
| creator | Perez-Vizcaino, Francisco Bishop-Bailley, David Lodi, Federica Duarte, Juan Cogolludo, Angel Moreno, Laura Bosca, Lisardo Mitchell, Jane A. Warner, Timothy D. |
| description | Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cells (VSMC) in culture. After 48
h, quercetin reduced the viability of a polyclonal intimal-type cell line derived from neonatal aorta but not of a medial-type cell line derived from adult aorta. These differential effects were similar in both proliferating and quiescent VSMC. Quercetin also preferentially reduced the viability of intimal-type over medial-type VSMC in primary cultures derived from balloon-injured carotid arteries. The effects of quercetin on cell viability were mainly dependent upon induction of apoptosis, as demonstrated by nuclear condensation and fragmentation, and were unrelated to PPARγ, pro-oxidant effects or nitric oxide. The expression of MAPKs (ERK, p38, and JNK) and ERK phosphorylation were not different between intimal- and medial-type VSMC. p38 phosphorylation was negligible in both cell types. Medial-type showed a weak JNK phosphorylation while this was markedly increased in intimal-type cells. Quercetin reduced JNK phosphorylation but had no consistent effect on ERK phosphorylation. In conclusion, quercetin preferentially produced apoptosis in intimal-type compared to medial-type VSMC. This might play a role in the anti-atherogenic and anti-hypertensive effects of quercetin. |
| doi_str_mv | 10.1016/j.bbrc.2006.05.198 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_20854394</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X06012812</els_id><sourcerecordid>68574116</sourcerecordid><originalsourceid>FETCH-LOGICAL-c479t-9a9b45f0bec15a964380eb759ecb8ab070bc274b702ea64efdafeb653c63541f3</originalsourceid><addsrcrecordid>eNqFkVGL1DAUhYMo7rj6B3yQgOBb602bJg34Isvqqou-rOBbSNJbJkPbjEk64L83ZQZ8cyFwQ-53Dzn3EPKaQc2AifeH2tro6gZA1NDVTPVPyI6BgqphwJ-SHZRO1Sj264q8SOkAwBgX6jm5YkJKCbLdkflhj3SczCkswQ_094rRYfYL9cuwOkzUHMMxh-TLbRnK695bnxP9-v0bNS77k8k-bHQ52c9moieT3DqZSNMcQt7TeU1uQupwmtJL8mw0U8JXl3pNfn66fbi5q-5_fP5y8_G-clyqXCmjLO9GsOhYZ5TgbQ9oZafQ2d5YkGBdI7mV0KARHMfBjGhF1zrRdpyN7TV5e9YNKXudnM_o9i4sC7qsG-g73ipeqHdn6hhDMZ6ynn3a_mkWDGvSou8kZ0w8CjLZiJZxKGBzBl0MKUUc9TGWpcQ_moHeMtMHvWWmt8w0dLpkVobeXNRXO-Pwb-QSUgE-nAEsKzt5jJsjXBwOPm6GhuD_p_8X7OypnQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17263140</pqid></control><display><type>article</type><title>The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Perez-Vizcaino, Francisco ; Bishop-Bailley, David ; Lodi, Federica ; Duarte, Juan ; Cogolludo, Angel ; Moreno, Laura ; Bosca, Lisardo ; Mitchell, Jane A. ; Warner, Timothy D.</creator><creatorcontrib>Perez-Vizcaino, Francisco ; Bishop-Bailley, David ; Lodi, Federica ; Duarte, Juan ; Cogolludo, Angel ; Moreno, Laura ; Bosca, Lisardo ; Mitchell, Jane A. ; Warner, Timothy D.</creatorcontrib><description>Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cells (VSMC) in culture. After 48
h, quercetin reduced the viability of a polyclonal intimal-type cell line derived from neonatal aorta but not of a medial-type cell line derived from adult aorta. These differential effects were similar in both proliferating and quiescent VSMC. Quercetin also preferentially reduced the viability of intimal-type over medial-type VSMC in primary cultures derived from balloon-injured carotid arteries. The effects of quercetin on cell viability were mainly dependent upon induction of apoptosis, as demonstrated by nuclear condensation and fragmentation, and were unrelated to PPARγ, pro-oxidant effects or nitric oxide. The expression of MAPKs (ERK, p38, and JNK) and ERK phosphorylation were not different between intimal- and medial-type VSMC. p38 phosphorylation was negligible in both cell types. Medial-type showed a weak JNK phosphorylation while this was markedly increased in intimal-type cells. Quercetin reduced JNK phosphorylation but had no consistent effect on ERK phosphorylation. In conclusion, quercetin preferentially produced apoptosis in intimal-type compared to medial-type VSMC. This might play a role in the anti-atherogenic and anti-hypertensive effects of quercetin.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.05.198</identifier><identifier>PMID: 16777073</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; AORTA ; APOPTOSIS ; Apoptosis - drug effects ; BLOOD PRESSURE ; CAROTID ARTERIES ; Cell Nucleus - drug effects ; Cell Shape ; Cells, Cultured ; Enzyme Activation - drug effects ; Flavonoid ; Intimal ; JNK ; JNK Mitogen-Activated Protein Kinases - metabolism ; Ligands ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - enzymology ; MUSCLES ; NITRIC OXIDE ; Nitric Oxide - biosynthesis ; OXIDIZERS ; PHOSPHORYLATION ; Phosphorylation - drug effects ; PPAR gamma - metabolism ; QUERCETIN ; Quercetin - pharmacology ; RATS ; Reactive Oxygen Species - metabolism ; Tunica Intima - cytology ; Vascular smooth muscle ; VASODILATORS</subject><ispartof>Biochemical and biophysical research communications, 2006-08, Vol.346 (3), p.919-925</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-9a9b45f0bec15a964380eb759ecb8ab070bc274b702ea64efdafeb653c63541f3</citedby><cites>FETCH-LOGICAL-c479t-9a9b45f0bec15a964380eb759ecb8ab070bc274b702ea64efdafeb653c63541f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2006.05.198$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16777073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20854394$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Perez-Vizcaino, Francisco</creatorcontrib><creatorcontrib>Bishop-Bailley, David</creatorcontrib><creatorcontrib>Lodi, Federica</creatorcontrib><creatorcontrib>Duarte, Juan</creatorcontrib><creatorcontrib>Cogolludo, Angel</creatorcontrib><creatorcontrib>Moreno, Laura</creatorcontrib><creatorcontrib>Bosca, Lisardo</creatorcontrib><creatorcontrib>Mitchell, Jane A.</creatorcontrib><creatorcontrib>Warner, Timothy D.</creatorcontrib><title>The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cells (VSMC) in culture. After 48
h, quercetin reduced the viability of a polyclonal intimal-type cell line derived from neonatal aorta but not of a medial-type cell line derived from adult aorta. These differential effects were similar in both proliferating and quiescent VSMC. Quercetin also preferentially reduced the viability of intimal-type over medial-type VSMC in primary cultures derived from balloon-injured carotid arteries. The effects of quercetin on cell viability were mainly dependent upon induction of apoptosis, as demonstrated by nuclear condensation and fragmentation, and were unrelated to PPARγ, pro-oxidant effects or nitric oxide. The expression of MAPKs (ERK, p38, and JNK) and ERK phosphorylation were not different between intimal- and medial-type VSMC. p38 phosphorylation was negligible in both cell types. Medial-type showed a weak JNK phosphorylation while this was markedly increased in intimal-type cells. Quercetin reduced JNK phosphorylation but had no consistent effect on ERK phosphorylation. In conclusion, quercetin preferentially produced apoptosis in intimal-type compared to medial-type VSMC. This might play a role in the anti-atherogenic and anti-hypertensive effects of quercetin.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>AORTA</subject><subject>APOPTOSIS</subject><subject>Apoptosis - drug effects</subject><subject>BLOOD PRESSURE</subject><subject>CAROTID ARTERIES</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Shape</subject><subject>Cells, Cultured</subject><subject>Enzyme Activation - drug effects</subject><subject>Flavonoid</subject><subject>Intimal</subject><subject>JNK</subject><subject>JNK Mitogen-Activated Protein Kinases - metabolism</subject><subject>Ligands</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - enzymology</subject><subject>MUSCLES</subject><subject>NITRIC OXIDE</subject><subject>Nitric Oxide - biosynthesis</subject><subject>OXIDIZERS</subject><subject>PHOSPHORYLATION</subject><subject>Phosphorylation - drug effects</subject><subject>PPAR gamma - metabolism</subject><subject>QUERCETIN</subject><subject>Quercetin - pharmacology</subject><subject>RATS</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Tunica Intima - cytology</subject><subject>Vascular smooth muscle</subject><subject>VASODILATORS</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVGL1DAUhYMo7rj6B3yQgOBb602bJg34Isvqqou-rOBbSNJbJkPbjEk64L83ZQZ8cyFwQ-53Dzn3EPKaQc2AifeH2tro6gZA1NDVTPVPyI6BgqphwJ-SHZRO1Sj264q8SOkAwBgX6jm5YkJKCbLdkflhj3SczCkswQ_094rRYfYL9cuwOkzUHMMxh-TLbRnK695bnxP9-v0bNS77k8k-bHQ52c9moieT3DqZSNMcQt7TeU1uQupwmtJL8mw0U8JXl3pNfn66fbi5q-5_fP5y8_G-clyqXCmjLO9GsOhYZ5TgbQ9oZafQ2d5YkGBdI7mV0KARHMfBjGhF1zrRdpyN7TV5e9YNKXudnM_o9i4sC7qsG-g73ipeqHdn6hhDMZ6ynn3a_mkWDGvSou8kZ0w8CjLZiJZxKGBzBl0MKUUc9TGWpcQ_moHeMtMHvWWmt8w0dLpkVobeXNRXO-Pwb-QSUgE-nAEsKzt5jJsjXBwOPm6GhuD_p_8X7OypnQ</recordid><startdate>20060804</startdate><enddate>20060804</enddate><creator>Perez-Vizcaino, Francisco</creator><creator>Bishop-Bailley, David</creator><creator>Lodi, Federica</creator><creator>Duarte, Juan</creator><creator>Cogolludo, Angel</creator><creator>Moreno, Laura</creator><creator>Bosca, Lisardo</creator><creator>Mitchell, Jane A.</creator><creator>Warner, Timothy D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20060804</creationdate><title>The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells</title><author>Perez-Vizcaino, Francisco ; Bishop-Bailley, David ; Lodi, Federica ; Duarte, Juan ; Cogolludo, Angel ; Moreno, Laura ; Bosca, Lisardo ; Mitchell, Jane A. ; Warner, Timothy D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-9a9b45f0bec15a964380eb759ecb8ab070bc274b702ea64efdafeb653c63541f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>AORTA</topic><topic>APOPTOSIS</topic><topic>Apoptosis - drug effects</topic><topic>BLOOD PRESSURE</topic><topic>CAROTID ARTERIES</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Shape</topic><topic>Cells, Cultured</topic><topic>Enzyme Activation - drug effects</topic><topic>Flavonoid</topic><topic>Intimal</topic><topic>JNK</topic><topic>JNK Mitogen-Activated Protein Kinases - metabolism</topic><topic>Ligands</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - enzymology</topic><topic>MUSCLES</topic><topic>NITRIC OXIDE</topic><topic>Nitric Oxide - biosynthesis</topic><topic>OXIDIZERS</topic><topic>PHOSPHORYLATION</topic><topic>Phosphorylation - drug effects</topic><topic>PPAR gamma - metabolism</topic><topic>QUERCETIN</topic><topic>Quercetin - pharmacology</topic><topic>RATS</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Tunica Intima - cytology</topic><topic>Vascular smooth muscle</topic><topic>VASODILATORS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perez-Vizcaino, Francisco</creatorcontrib><creatorcontrib>Bishop-Bailley, David</creatorcontrib><creatorcontrib>Lodi, Federica</creatorcontrib><creatorcontrib>Duarte, Juan</creatorcontrib><creatorcontrib>Cogolludo, Angel</creatorcontrib><creatorcontrib>Moreno, Laura</creatorcontrib><creatorcontrib>Bosca, Lisardo</creatorcontrib><creatorcontrib>Mitchell, Jane A.</creatorcontrib><creatorcontrib>Warner, Timothy D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perez-Vizcaino, Francisco</au><au>Bishop-Bailley, David</au><au>Lodi, Federica</au><au>Duarte, Juan</au><au>Cogolludo, Angel</au><au>Moreno, Laura</au><au>Bosca, Lisardo</au><au>Mitchell, Jane A.</au><au>Warner, Timothy D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-08-04</date><risdate>2006</risdate><volume>346</volume><issue>3</issue><spage>919</spage><epage>925</epage><pages>919-925</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Quercetin, the most abundant dietary flavonol, exerts vasodilator, anti-hypertensive, and anti-atherogenic effects and reduces the vascular remodelling associated with elevated blood pressure. Here, we have compared the effects of quercetin in intimal- and medial-type rat vascular smooth muscle cells (VSMC) in culture. After 48
h, quercetin reduced the viability of a polyclonal intimal-type cell line derived from neonatal aorta but not of a medial-type cell line derived from adult aorta. These differential effects were similar in both proliferating and quiescent VSMC. Quercetin also preferentially reduced the viability of intimal-type over medial-type VSMC in primary cultures derived from balloon-injured carotid arteries. The effects of quercetin on cell viability were mainly dependent upon induction of apoptosis, as demonstrated by nuclear condensation and fragmentation, and were unrelated to PPARγ, pro-oxidant effects or nitric oxide. The expression of MAPKs (ERK, p38, and JNK) and ERK phosphorylation were not different between intimal- and medial-type VSMC. p38 phosphorylation was negligible in both cell types. Medial-type showed a weak JNK phosphorylation while this was markedly increased in intimal-type cells. Quercetin reduced JNK phosphorylation but had no consistent effect on ERK phosphorylation. In conclusion, quercetin preferentially produced apoptosis in intimal-type compared to medial-type VSMC. This might play a role in the anti-atherogenic and anti-hypertensive effects of quercetin.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16777073</pmid><doi>10.1016/j.bbrc.2006.05.198</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2006-08, Vol.346 (3), p.919-925 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_osti_scitechconnect_20854394 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 60 APPLIED LIFE SCIENCES Animals AORTA APOPTOSIS Apoptosis - drug effects BLOOD PRESSURE CAROTID ARTERIES Cell Nucleus - drug effects Cell Shape Cells, Cultured Enzyme Activation - drug effects Flavonoid Intimal JNK JNK Mitogen-Activated Protein Kinases - metabolism Ligands Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - enzymology MUSCLES NITRIC OXIDE Nitric Oxide - biosynthesis OXIDIZERS PHOSPHORYLATION Phosphorylation - drug effects PPAR gamma - metabolism QUERCETIN Quercetin - pharmacology RATS Reactive Oxygen Species - metabolism Tunica Intima - cytology Vascular smooth muscle VASODILATORS |
| title | The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T14%3A41%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20flavonoid%20quercetin%20induces%20apoptosis%20and%20inhibits%20JNK%20activation%20in%20intimal%20vascular%20smooth%20muscle%20cells&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Perez-Vizcaino,%20Francisco&rft.date=2006-08-04&rft.volume=346&rft.issue=3&rft.spage=919&rft.epage=925&rft.pages=919-925&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2006.05.198&rft_dat=%3Cproquest_osti_%3E68574116%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17263140&rft_id=info:pmid/16777073&rft_els_id=S0006291X06012812&rfr_iscdi=true |