Naked gene therapy of hepatocyte growth factor for dextran sulfate sodium-induced colitis in mice
Ulcerative colitis (UC) is progressive and relapsing disease. To explore the therapeutic effects of naked gene therapy of hepatocyte growth factor (HGF) on UC, the SRα promoter driving HGF gene was intrarectally administered to the mice in which colitis was induced by dextran sulfate sodium (DSS). E...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-07, Vol.345 (4), p.1517-1525 |
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| creator | Kanbe, Takamasa Murai, Rie Mukoyama, Tomoyuki Murawaki, Yoshiyuki Hashiguchi, Ko-ichi Yoshida, Yoko Tsuchiya, Hiroyuki Kurimasa, Akihiro Harada, Ken-ichi Yashima, Kazuo Nishimuki, Eiji Shabana, Noriko Kishimoto, Yukihiro Kojyo, Haruhiko Miura, Kunihiko Murawaki, Yoshikazu Kawasaki, Hironaka Shiota, Goshi |
| description | Ulcerative colitis (UC) is progressive and relapsing disease. To explore the therapeutic effects of naked gene therapy of hepatocyte growth factor (HGF) on UC, the SRα promoter driving HGF gene was intrarectally administered to the mice in which colitis was induced by dextran sulfate sodium (DSS). Expression of the transgene was seen in surface epithelium, lamina propria, and muscularis mucosae. The HGF-treated mice showed reduced colonic mucosal damage and increased body weights, compared with control mice (
P
<
0.01 and
P
<
0.05, respectively). The HGF-treated mice displayed increased number of PCNA-positive cells and decreased number of apoptotic cells than in control mice (
P
<
0.01, each). Phosphorylated AKT was dramatically increased after HGF gene administration, however, phosphorylated ERK1/2 was not altered. Microarray analysis revealed that HGF induced expression of proliferation- and apoptosis-associated genes. These data suggest that naked HGF gene delivery causes therapeutic effects through regulation of many downstream genes. |
| doi_str_mv | 10.1016/j.bbrc.2006.05.084 |
| format | Article |
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P
<
0.01 and
P
<
0.05, respectively). The HGF-treated mice displayed increased number of PCNA-positive cells and decreased number of apoptotic cells than in control mice (
P
<
0.01, each). Phosphorylated AKT was dramatically increased after HGF gene administration, however, phosphorylated ERK1/2 was not altered. Microarray analysis revealed that HGF induced expression of proliferation- and apoptosis-associated genes. These data suggest that naked HGF gene delivery causes therapeutic effects through regulation of many downstream genes.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.05.084</identifier><identifier>PMID: 16735026</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; Apoptosis ; Body Weight ; CELL PROLIFERATION ; Colitis - chemically induced ; Colitis - genetics ; Colitis - therapy ; Colon - metabolism ; Colon - pathology ; DEXTRAN ; Dextran Sulfate ; DNA ; EPITHELIUM ; Female ; Gene Expression ; Gene Expression Profiling ; GENE REGULATION ; GENE THERAPY ; GENES ; Genetic Therapy - methods ; GROWTH FACTORS ; Hepatocyte growth factor ; Hepatocyte Growth Factor - genetics ; Hepatocyte Growth Factor - physiology ; Immunoblotting ; MICE ; Mice, Inbred BALB C ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 - metabolism ; Naked DNA ; Oligonucleotide Array Sequence Analysis ; Phosphorylation ; Proto-Oncogene Proteins c-akt - metabolism ; RECTAL ADMINISTRATION ; SODIUM ; SULFATES ; Ulcerative colitis ; URANIUM CARBIDES</subject><ispartof>Biochemical and biophysical research communications, 2006-07, Vol.345 (4), p.1517-1525</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-9d88ad0cbc04ee660c4a12fa3fe9e5d45f598e6913c6c4fcd6c2e968597137433</citedby><cites>FETCH-LOGICAL-c523t-9d88ad0cbc04ee660c4a12fa3fe9e5d45f598e6913c6c4fcd6c2e968597137433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X06010989$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16735026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20854358$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanbe, Takamasa</creatorcontrib><creatorcontrib>Murai, Rie</creatorcontrib><creatorcontrib>Mukoyama, Tomoyuki</creatorcontrib><creatorcontrib>Murawaki, Yoshiyuki</creatorcontrib><creatorcontrib>Hashiguchi, Ko-ichi</creatorcontrib><creatorcontrib>Yoshida, Yoko</creatorcontrib><creatorcontrib>Tsuchiya, Hiroyuki</creatorcontrib><creatorcontrib>Kurimasa, Akihiro</creatorcontrib><creatorcontrib>Harada, Ken-ichi</creatorcontrib><creatorcontrib>Yashima, Kazuo</creatorcontrib><creatorcontrib>Nishimuki, Eiji</creatorcontrib><creatorcontrib>Shabana, Noriko</creatorcontrib><creatorcontrib>Kishimoto, Yukihiro</creatorcontrib><creatorcontrib>Kojyo, Haruhiko</creatorcontrib><creatorcontrib>Miura, Kunihiko</creatorcontrib><creatorcontrib>Murawaki, Yoshikazu</creatorcontrib><creatorcontrib>Kawasaki, Hironaka</creatorcontrib><creatorcontrib>Shiota, Goshi</creatorcontrib><title>Naked gene therapy of hepatocyte growth factor for dextran sulfate sodium-induced colitis in mice</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Ulcerative colitis (UC) is progressive and relapsing disease. To explore the therapeutic effects of naked gene therapy of hepatocyte growth factor (HGF) on UC, the SRα promoter driving HGF gene was intrarectally administered to the mice in which colitis was induced by dextran sulfate sodium (DSS). Expression of the transgene was seen in surface epithelium, lamina propria, and muscularis mucosae. The HGF-treated mice showed reduced colonic mucosal damage and increased body weights, compared with control mice (
P
<
0.01 and
P
<
0.05, respectively). The HGF-treated mice displayed increased number of PCNA-positive cells and decreased number of apoptotic cells than in control mice (
P
<
0.01, each). Phosphorylated AKT was dramatically increased after HGF gene administration, however, phosphorylated ERK1/2 was not altered. Microarray analysis revealed that HGF induced expression of proliferation- and apoptosis-associated genes. These data suggest that naked HGF gene delivery causes therapeutic effects through regulation of many downstream genes.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Body Weight</subject><subject>CELL PROLIFERATION</subject><subject>Colitis - chemically induced</subject><subject>Colitis - genetics</subject><subject>Colitis - therapy</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>DEXTRAN</subject><subject>Dextran Sulfate</subject><subject>DNA</subject><subject>EPITHELIUM</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>GENE REGULATION</subject><subject>GENE THERAPY</subject><subject>GENES</subject><subject>Genetic Therapy - methods</subject><subject>GROWTH FACTORS</subject><subject>Hepatocyte growth factor</subject><subject>Hepatocyte Growth Factor - genetics</subject><subject>Hepatocyte Growth Factor - physiology</subject><subject>Immunoblotting</subject><subject>MICE</subject><subject>Mice, Inbred BALB C</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>Naked DNA</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Phosphorylation</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>RECTAL ADMINISTRATION</subject><subject>SODIUM</subject><subject>SULFATES</subject><subject>Ulcerative colitis</subject><subject>URANIUM CARBIDES</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGKFDEQhoMo7uzqC3iQgOCt2ySdZDrgRRZdhUUvCt5CplK9k7G7MyZpdd7eNDPgzUNRh_r-n-Ij5AVnLWdcvzm0u12CVjCmW6Za1stHZMOZYY3gTD4mG1YvjTD8-xW5zvnAGOdSm6fkiuttp5jQG-I-ux_o6QPOSMsekzueaBzoHo-uRDgVpA8p_i57OjgoMdGhjsc_JbmZ5mUcXCVy9GGZmjD7BWoXxDGUkGmY6RQAn5EngxszPr_sG_Ltw_uvtx-b-y93n27f3TegRFca4_veeQY7YBJRawbScTG4bkCDyks1KNOjNrwDDXIAr0Gg0b0yW95tZdfdkFfn3phLsBlCQdhDnGeEYgXrlexUX6nXZ-qY4s8Fc7FTyIDj6GaMS7bc9LJXQlVQnEFIMeeEgz2mMLl0spzZVb892FW_XfVbpmzVX0MvL-3LbkL_L3LxXYG3ZwCriV8B0_oozlVbSOufPob_9f8FMi2W8g</recordid><startdate>20060714</startdate><enddate>20060714</enddate><creator>Kanbe, Takamasa</creator><creator>Murai, Rie</creator><creator>Mukoyama, Tomoyuki</creator><creator>Murawaki, Yoshiyuki</creator><creator>Hashiguchi, Ko-ichi</creator><creator>Yoshida, Yoko</creator><creator>Tsuchiya, Hiroyuki</creator><creator>Kurimasa, Akihiro</creator><creator>Harada, Ken-ichi</creator><creator>Yashima, Kazuo</creator><creator>Nishimuki, Eiji</creator><creator>Shabana, Noriko</creator><creator>Kishimoto, Yukihiro</creator><creator>Kojyo, Haruhiko</creator><creator>Miura, Kunihiko</creator><creator>Murawaki, Yoshikazu</creator><creator>Kawasaki, Hironaka</creator><creator>Shiota, Goshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope></search><sort><creationdate>20060714</creationdate><title>Naked gene therapy of hepatocyte growth factor for dextran sulfate sodium-induced colitis in mice</title><author>Kanbe, Takamasa ; Murai, Rie ; Mukoyama, Tomoyuki ; Murawaki, Yoshiyuki ; Hashiguchi, Ko-ichi ; Yoshida, Yoko ; Tsuchiya, Hiroyuki ; Kurimasa, Akihiro ; Harada, Ken-ichi ; Yashima, Kazuo ; Nishimuki, Eiji ; Shabana, Noriko ; Kishimoto, Yukihiro ; Kojyo, Haruhiko ; Miura, Kunihiko ; Murawaki, Yoshikazu ; Kawasaki, Hironaka ; Shiota, Goshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-9d88ad0cbc04ee660c4a12fa3fe9e5d45f598e6913c6c4fcd6c2e968597137433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Body Weight</topic><topic>CELL PROLIFERATION</topic><topic>Colitis - chemically induced</topic><topic>Colitis - genetics</topic><topic>Colitis - therapy</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>DEXTRAN</topic><topic>Dextran Sulfate</topic><topic>DNA</topic><topic>EPITHELIUM</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>GENE REGULATION</topic><topic>GENE THERAPY</topic><topic>GENES</topic><topic>Genetic Therapy - methods</topic><topic>GROWTH FACTORS</topic><topic>Hepatocyte growth factor</topic><topic>Hepatocyte Growth Factor - genetics</topic><topic>Hepatocyte Growth Factor - physiology</topic><topic>Immunoblotting</topic><topic>MICE</topic><topic>Mice, Inbred BALB C</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>Naked DNA</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Phosphorylation</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>RECTAL ADMINISTRATION</topic><topic>SODIUM</topic><topic>SULFATES</topic><topic>Ulcerative colitis</topic><topic>URANIUM CARBIDES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanbe, Takamasa</creatorcontrib><creatorcontrib>Murai, Rie</creatorcontrib><creatorcontrib>Mukoyama, Tomoyuki</creatorcontrib><creatorcontrib>Murawaki, Yoshiyuki</creatorcontrib><creatorcontrib>Hashiguchi, Ko-ichi</creatorcontrib><creatorcontrib>Yoshida, Yoko</creatorcontrib><creatorcontrib>Tsuchiya, Hiroyuki</creatorcontrib><creatorcontrib>Kurimasa, Akihiro</creatorcontrib><creatorcontrib>Harada, Ken-ichi</creatorcontrib><creatorcontrib>Yashima, Kazuo</creatorcontrib><creatorcontrib>Nishimuki, Eiji</creatorcontrib><creatorcontrib>Shabana, Noriko</creatorcontrib><creatorcontrib>Kishimoto, Yukihiro</creatorcontrib><creatorcontrib>Kojyo, Haruhiko</creatorcontrib><creatorcontrib>Miura, Kunihiko</creatorcontrib><creatorcontrib>Murawaki, Yoshikazu</creatorcontrib><creatorcontrib>Kawasaki, Hironaka</creatorcontrib><creatorcontrib>Shiota, Goshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanbe, Takamasa</au><au>Murai, Rie</au><au>Mukoyama, Tomoyuki</au><au>Murawaki, Yoshiyuki</au><au>Hashiguchi, Ko-ichi</au><au>Yoshida, Yoko</au><au>Tsuchiya, Hiroyuki</au><au>Kurimasa, Akihiro</au><au>Harada, Ken-ichi</au><au>Yashima, Kazuo</au><au>Nishimuki, Eiji</au><au>Shabana, Noriko</au><au>Kishimoto, Yukihiro</au><au>Kojyo, Haruhiko</au><au>Miura, Kunihiko</au><au>Murawaki, Yoshikazu</au><au>Kawasaki, Hironaka</au><au>Shiota, Goshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Naked gene therapy of hepatocyte growth factor for dextran sulfate sodium-induced colitis in mice</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-07-14</date><risdate>2006</risdate><volume>345</volume><issue>4</issue><spage>1517</spage><epage>1525</epage><pages>1517-1525</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Ulcerative colitis (UC) is progressive and relapsing disease. To explore the therapeutic effects of naked gene therapy of hepatocyte growth factor (HGF) on UC, the SRα promoter driving HGF gene was intrarectally administered to the mice in which colitis was induced by dextran sulfate sodium (DSS). Expression of the transgene was seen in surface epithelium, lamina propria, and muscularis mucosae. The HGF-treated mice showed reduced colonic mucosal damage and increased body weights, compared with control mice (
P
<
0.01 and
P
<
0.05, respectively). The HGF-treated mice displayed increased number of PCNA-positive cells and decreased number of apoptotic cells than in control mice (
P
<
0.01, each). Phosphorylated AKT was dramatically increased after HGF gene administration, however, phosphorylated ERK1/2 was not altered. Microarray analysis revealed that HGF induced expression of proliferation- and apoptosis-associated genes. These data suggest that naked HGF gene delivery causes therapeutic effects through regulation of many downstream genes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16735026</pmid><doi>10.1016/j.bbrc.2006.05.084</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2006-07, Vol.345 (4), p.1517-1525 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 60 APPLIED LIFE SCIENCES Animals Apoptosis Body Weight CELL PROLIFERATION Colitis - chemically induced Colitis - genetics Colitis - therapy Colon - metabolism Colon - pathology DEXTRAN Dextran Sulfate DNA EPITHELIUM Female Gene Expression Gene Expression Profiling GENE REGULATION GENE THERAPY GENES Genetic Therapy - methods GROWTH FACTORS Hepatocyte growth factor Hepatocyte Growth Factor - genetics Hepatocyte Growth Factor - physiology Immunoblotting MICE Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - metabolism Naked DNA Oligonucleotide Array Sequence Analysis Phosphorylation Proto-Oncogene Proteins c-akt - metabolism RECTAL ADMINISTRATION SODIUM SULFATES Ulcerative colitis URANIUM CARBIDES |
| title | Naked gene therapy of hepatocyte growth factor for dextran sulfate sodium-induced colitis in mice |
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