ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion
We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of m...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2006-06, Vol.345 (1), p.216-221 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 221 |
|---|---|
| container_issue | 1 |
| container_start_page | 216 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 345 |
| creator | Moss, Alan C. Lawlor, Garrett Murray, David Tighe, Dónal Madden, Stephen F. Mulligan, Anne-Marie Keane, Conor O. Brady, Hugh R. Doran, Peter P. MacMathuna, Padraic |
| description | We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue (
p
<
0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation (
p
<
0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation (
p
<
0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention. |
| doi_str_mv | 10.1016/j.bbrc.2006.04.094 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_20854312</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X06009387</els_id><sourcerecordid>67978668</sourcerecordid><originalsourceid>FETCH-LOGICAL-c479t-c69f9eb169ce7885dcb9bdbaa71ce8dd6c657411b28621a3155ac96c2c448bae3</originalsourceid><addsrcrecordid>eNqFkdFrFDEQxoNY7PX0H_BBFoS-7TaTy2YT8EVKrUKLIFV8C9nZOcx1LzmTvSv9781yB77p0zDMbz7mm4-xt8Ab4KCuNk3fJ2wE56rhsuFGvmAL4IbXArh8yRa8TGph4Oc5u8h5wzmAVOYVOwelOg1itWDfbh5-yMqFobp_pnioHkPExyE-hcpvd86nXGEcY6jQBaRUIY1jNfpA1S7F0a8pucmX8Szgw8Hl0rxmZ2s3Znpzqkv2_dPNw_Xn-u7r7Zfrj3c1ys5MNSqzNtSDMkid1u2AvemH3rkOkPQwKFRtJwF6oZUAt4K2dWgUCpRS945WS_b-qBvz5G1GPxH-whgC4WQF161cFYtLdnmkysG_95Qnu_V5tuECxX22qjOdVkr_F4ROgFZSFlAcQUwx50Rru0t-69KzBW7nYOzGzsHYORjLpS3BlKV3J_V9v6Xh78opiQJ8OAJUXnbwlGZHVJ4--DQbGqL_l_4fdgSevQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17218644</pqid></control><display><type>article</type><title>ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Moss, Alan C. ; Lawlor, Garrett ; Murray, David ; Tighe, Dónal ; Madden, Stephen F. ; Mulligan, Anne-Marie ; Keane, Conor O. ; Brady, Hugh R. ; Doran, Peter P. ; MacMathuna, Padraic</creator><creatorcontrib>Moss, Alan C. ; Lawlor, Garrett ; Murray, David ; Tighe, Dónal ; Madden, Stephen F. ; Mulligan, Anne-Marie ; Keane, Conor O. ; Brady, Hugh R. ; Doran, Peter P. ; MacMathuna, Padraic</creatorcontrib><description>We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue (
p
<
0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation (
p
<
0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation (
p
<
0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.04.094</identifier><identifier>PMID: 16678123</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Adenovirus E1A Proteins - genetics ; Adenovirus E1A Proteins - metabolism ; CARCINOMAS ; Cell invasion ; Cell Line, Tumor ; CELL PROLIFERATION ; Colon cancer ; Colonic Neoplasms - pathology ; Colonic Neoplasms - physiopathology ; Digital Differential Display ; ETV4 ; Gene Silencing ; GENES ; Humans ; LARGE INTESTINE ; Myeov ; Neoplasm Invasiveness ; POLYMERASE CHAIN REACTION ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2006-06, Vol.345 (1), p.216-221</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-c69f9eb169ce7885dcb9bdbaa71ce8dd6c657411b28621a3155ac96c2c448bae3</citedby><cites>FETCH-LOGICAL-c479t-c69f9eb169ce7885dcb9bdbaa71ce8dd6c657411b28621a3155ac96c2c448bae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X06009387$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16678123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20854312$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Moss, Alan C.</creatorcontrib><creatorcontrib>Lawlor, Garrett</creatorcontrib><creatorcontrib>Murray, David</creatorcontrib><creatorcontrib>Tighe, Dónal</creatorcontrib><creatorcontrib>Madden, Stephen F.</creatorcontrib><creatorcontrib>Mulligan, Anne-Marie</creatorcontrib><creatorcontrib>Keane, Conor O.</creatorcontrib><creatorcontrib>Brady, Hugh R.</creatorcontrib><creatorcontrib>Doran, Peter P.</creatorcontrib><creatorcontrib>MacMathuna, Padraic</creatorcontrib><title>ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue (
p
<
0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation (
p
<
0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation (
p
<
0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Adenovirus E1A Proteins - genetics</subject><subject>Adenovirus E1A Proteins - metabolism</subject><subject>CARCINOMAS</subject><subject>Cell invasion</subject><subject>Cell Line, Tumor</subject><subject>CELL PROLIFERATION</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colonic Neoplasms - physiopathology</subject><subject>Digital Differential Display</subject><subject>ETV4</subject><subject>Gene Silencing</subject><subject>GENES</subject><subject>Humans</subject><subject>LARGE INTESTINE</subject><subject>Myeov</subject><subject>Neoplasm Invasiveness</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFrFDEQxoNY7PX0H_BBFoS-7TaTy2YT8EVKrUKLIFV8C9nZOcx1LzmTvSv9781yB77p0zDMbz7mm4-xt8Ab4KCuNk3fJ2wE56rhsuFGvmAL4IbXArh8yRa8TGph4Oc5u8h5wzmAVOYVOwelOg1itWDfbh5-yMqFobp_pnioHkPExyE-hcpvd86nXGEcY6jQBaRUIY1jNfpA1S7F0a8pucmX8Szgw8Hl0rxmZ2s3Znpzqkv2_dPNw_Xn-u7r7Zfrj3c1ys5MNSqzNtSDMkid1u2AvemH3rkOkPQwKFRtJwF6oZUAt4K2dWgUCpRS945WS_b-qBvz5G1GPxH-whgC4WQF161cFYtLdnmkysG_95Qnu_V5tuECxX22qjOdVkr_F4ROgFZSFlAcQUwx50Rru0t-69KzBW7nYOzGzsHYORjLpS3BlKV3J_V9v6Xh78opiQJ8OAJUXnbwlGZHVJ4--DQbGqL_l_4fdgSevQ</recordid><startdate>20060623</startdate><enddate>20060623</enddate><creator>Moss, Alan C.</creator><creator>Lawlor, Garrett</creator><creator>Murray, David</creator><creator>Tighe, Dónal</creator><creator>Madden, Stephen F.</creator><creator>Mulligan, Anne-Marie</creator><creator>Keane, Conor O.</creator><creator>Brady, Hugh R.</creator><creator>Doran, Peter P.</creator><creator>MacMathuna, Padraic</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20060623</creationdate><title>ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion</title><author>Moss, Alan C. ; Lawlor, Garrett ; Murray, David ; Tighe, Dónal ; Madden, Stephen F. ; Mulligan, Anne-Marie ; Keane, Conor O. ; Brady, Hugh R. ; Doran, Peter P. ; MacMathuna, Padraic</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-c69f9eb169ce7885dcb9bdbaa71ce8dd6c657411b28621a3155ac96c2c448bae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Adenovirus E1A Proteins - genetics</topic><topic>Adenovirus E1A Proteins - metabolism</topic><topic>CARCINOMAS</topic><topic>Cell invasion</topic><topic>Cell Line, Tumor</topic><topic>CELL PROLIFERATION</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colonic Neoplasms - physiopathology</topic><topic>Digital Differential Display</topic><topic>ETV4</topic><topic>Gene Silencing</topic><topic>GENES</topic><topic>Humans</topic><topic>LARGE INTESTINE</topic><topic>Myeov</topic><topic>Neoplasm Invasiveness</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moss, Alan C.</creatorcontrib><creatorcontrib>Lawlor, Garrett</creatorcontrib><creatorcontrib>Murray, David</creatorcontrib><creatorcontrib>Tighe, Dónal</creatorcontrib><creatorcontrib>Madden, Stephen F.</creatorcontrib><creatorcontrib>Mulligan, Anne-Marie</creatorcontrib><creatorcontrib>Keane, Conor O.</creatorcontrib><creatorcontrib>Brady, Hugh R.</creatorcontrib><creatorcontrib>Doran, Peter P.</creatorcontrib><creatorcontrib>MacMathuna, Padraic</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moss, Alan C.</au><au>Lawlor, Garrett</au><au>Murray, David</au><au>Tighe, Dónal</au><au>Madden, Stephen F.</au><au>Mulligan, Anne-Marie</au><au>Keane, Conor O.</au><au>Brady, Hugh R.</au><au>Doran, Peter P.</au><au>MacMathuna, Padraic</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-06-23</date><risdate>2006</risdate><volume>345</volume><issue>1</issue><spage>216</spage><epage>221</epage><pages>216-221</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue (
p
<
0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation (
p
<
0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation (
p
<
0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16678123</pmid><doi>10.1016/j.bbrc.2006.04.094</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2006-06, Vol.345 (1), p.216-221 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_osti_scitechconnect_20854312 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 60 APPLIED LIFE SCIENCES Adenovirus E1A Proteins - genetics Adenovirus E1A Proteins - metabolism CARCINOMAS Cell invasion Cell Line, Tumor CELL PROLIFERATION Colon cancer Colonic Neoplasms - pathology Colonic Neoplasms - physiopathology Digital Differential Display ETV4 Gene Silencing GENES Humans LARGE INTESTINE Myeov Neoplasm Invasiveness POLYMERASE CHAIN REACTION Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism |
| title | ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T23%3A13%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ETV4%20and%20Myeov%20knockdown%20impairs%20colon%20cancer%20cell%20line%20proliferation%20and%20invasion&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Moss,%20Alan%20C.&rft.date=2006-06-23&rft.volume=345&rft.issue=1&rft.spage=216&rft.epage=221&rft.pages=216-221&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2006.04.094&rft_dat=%3Cproquest_osti_%3E67978668%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17218644&rft_id=info:pmid/16678123&rft_els_id=S0006291X06009387&rfr_iscdi=true |