Evaluation of Remediation of Coal Mining Wastewater by Chitosan Microspheres Using Biomarkers
Acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. Therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. The aim of this...
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| Veröffentlicht in: | Archives of environmental contamination and toxicology 2006-11, Vol.51 (4), p.633-640 |
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| creator | Benassi, J. C Laus, R Geremias, R Lima, P. L Menezes, C. T. B Laranjeira, M. C. M Wilhelm-Filho, D Fávere, V. T Pedrosa, R. C |
| description | Acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. Therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. The aim of this work was to evaluate the remediation of mining wastewater effluents by chitosan microspheres using biomarkers of exposure and effect. DNA damage (Comet assay) and several biomarkers of oxidative stress, such as lipoperoxidation levels (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities, and contents of reduced glutathione (GSH), were measured in blood and liver of tilapia (Oreochromis niloticus) exposed for 7, 15, and 30 days to dechlorinated tap water, 10% coal mining wastewater (CMW), and coal mining wastewater treated with chitosan microspheres (RCM). The results indicate that hepatic TBARS levels were significantly higher in fish exposed to CMW after 7, 15, and 30 days (100%, 86%, and 63%, respectively), and after remediation there was no significant difference in relation to the control group. Hepatic GSH concentrations were lower than control values for CMW after 7 and 15 days of exposure (34% decrease at both times), and this concentration was normalized by treatment with chitosan. SOD showed increased activity in liver after 15 and 30 days of exposure, 30% and 36%, respectively, and in fish exposed to RCM there was no change in this activity compared with the control group. Increased CAT activity in liver was observed during all experimental periods in fish exposed to CMW (46%, 50%, and 56% at 7, 15, and 30 days, respectively) compared with the control or treated-water groups. The highest increase in hepatic GST activity (106%) was observed only in fish exposed to CMW for 30 days. There was an increase in DNA damage in liver (50% at 7 and 15 days) and blood (79%, 77%, and 48% at 7, 15, and 30 days, respectively) after exposure to CMW. In contrast, the fish exposed to wastewater treated with chitosan microspheres exhibited DNA fragmentation indexes similar to the control group. The results obtained indicate the use of oxidative stress biomarkers as useful tools for the toxicity evaluation of coal mining effluents and also suggest that chitosan microspheres may be used as an alternative approach for remediation of coal mining wastewaters. |
| doi_str_mv | 10.1007/s00244-005-0187-4 |
| format | Article |
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C ; Laus, R ; Geremias, R ; Lima, P. L ; Menezes, C. T. B ; Laranjeira, M. C. M ; Wilhelm-Filho, D ; Fávere, V. T ; Pedrosa, R. C</creator><creatorcontrib>Benassi, J. C ; Laus, R ; Geremias, R ; Lima, P. L ; Menezes, C. T. B ; Laranjeira, M. C. M ; Wilhelm-Filho, D ; Fávere, V. T ; Pedrosa, R. C</creatorcontrib><description>Acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. Therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. The aim of this work was to evaluate the remediation of mining wastewater effluents by chitosan microspheres using biomarkers of exposure and effect. DNA damage (Comet assay) and several biomarkers of oxidative stress, such as lipoperoxidation levels (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities, and contents of reduced glutathione (GSH), were measured in blood and liver of tilapia (Oreochromis niloticus) exposed for 7, 15, and 30 days to dechlorinated tap water, 10% coal mining wastewater (CMW), and coal mining wastewater treated with chitosan microspheres (RCM). The results indicate that hepatic TBARS levels were significantly higher in fish exposed to CMW after 7, 15, and 30 days (100%, 86%, and 63%, respectively), and after remediation there was no significant difference in relation to the control group. Hepatic GSH concentrations were lower than control values for CMW after 7 and 15 days of exposure (34% decrease at both times), and this concentration was normalized by treatment with chitosan. SOD showed increased activity in liver after 15 and 30 days of exposure, 30% and 36%, respectively, and in fish exposed to RCM there was no change in this activity compared with the control group. Increased CAT activity in liver was observed during all experimental periods in fish exposed to CMW (46%, 50%, and 56% at 7, 15, and 30 days, respectively) compared with the control or treated-water groups. The highest increase in hepatic GST activity (106%) was observed only in fish exposed to CMW for 30 days. There was an increase in DNA damage in liver (50% at 7 and 15 days) and blood (79%, 77%, and 48% at 7, 15, and 30 days, respectively) after exposure to CMW. In contrast, the fish exposed to wastewater treated with chitosan microspheres exhibited DNA fragmentation indexes similar to the control group. The results obtained indicate the use of oxidative stress biomarkers as useful tools for the toxicity evaluation of coal mining effluents and also suggest that chitosan microspheres may be used as an alternative approach for remediation of coal mining wastewaters.</description><identifier>ISSN: 0090-4341</identifier><identifier>EISSN: 1432-0703</identifier><identifier>DOI: 10.1007/s00244-005-0187-4</identifier><identifier>PMID: 16988869</identifier><identifier>CODEN: AECTCV</identifier><language>eng</language><publisher>Heidelberg: New York : Springer-Verlag</publisher><subject>01 COAL, LIGNITE, AND PEAT ; ACID MINE DRAINAGE ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Applied sciences ; Bioassays ; Biological and medical sciences ; BIOLOGICAL MARKERS ; Biomarkers ; Blood ; Catalase - physiology ; CHITIN ; chitosan ; Chitosan - administration & dosage ; Cichlids ; coal ; Coal Mining ; Deoxyribonucleic acid ; DNA ; DNA Damage ; Drinking water ; Ecotoxicology, biological effects of pollution ; Effluents ; Environmental degradation ; Environmental Restoration and Remediation - methods ; EVALUATION ; Exact sciences and technology ; Fish ; FISHES ; Freshwater ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glutathione - analysis ; Hydrogen-Ion Concentration ; Lipid Peroxidation ; Liver ; Microspheres ; Oreochromis niloticus ; Oxidative Stress ; Pollution ; Reactive Oxygen Species - metabolism ; REMEDIAL ACTION ; Remediation ; TOXICITY ; Waste Disposal, Fluid - methods ; WASTE WATER ; Wastewater treatment ; Wastewaters ; Water monitoring ; Water Purification - methods ; Water quality ; Water quality management ; WATER TREATMENT ; Water treatment and pollution</subject><ispartof>Archives of environmental contamination and toxicology, 2006-11, Vol.51 (4), p.633-640</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer Science+Business Media, Inc. 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-3dbf1cf688f57e9bf725e85ebc88346f11976331ad2d86fa862e3416a90c6b0b3</citedby><cites>FETCH-LOGICAL-c470t-3dbf1cf688f57e9bf725e85ebc88346f11976331ad2d86fa862e3416a90c6b0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18418470$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16988869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20813316$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Benassi, J. C</creatorcontrib><creatorcontrib>Laus, R</creatorcontrib><creatorcontrib>Geremias, R</creatorcontrib><creatorcontrib>Lima, P. L</creatorcontrib><creatorcontrib>Menezes, C. T. B</creatorcontrib><creatorcontrib>Laranjeira, M. C. M</creatorcontrib><creatorcontrib>Wilhelm-Filho, D</creatorcontrib><creatorcontrib>Fávere, V. T</creatorcontrib><creatorcontrib>Pedrosa, R. C</creatorcontrib><title>Evaluation of Remediation of Coal Mining Wastewater by Chitosan Microspheres Using Biomarkers</title><title>Archives of environmental contamination and toxicology</title><addtitle>Arch Environ Contam Toxicol</addtitle><description>Acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. Therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. The aim of this work was to evaluate the remediation of mining wastewater effluents by chitosan microspheres using biomarkers of exposure and effect. DNA damage (Comet assay) and several biomarkers of oxidative stress, such as lipoperoxidation levels (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities, and contents of reduced glutathione (GSH), were measured in blood and liver of tilapia (Oreochromis niloticus) exposed for 7, 15, and 30 days to dechlorinated tap water, 10% coal mining wastewater (CMW), and coal mining wastewater treated with chitosan microspheres (RCM). The results indicate that hepatic TBARS levels were significantly higher in fish exposed to CMW after 7, 15, and 30 days (100%, 86%, and 63%, respectively), and after remediation there was no significant difference in relation to the control group. Hepatic GSH concentrations were lower than control values for CMW after 7 and 15 days of exposure (34% decrease at both times), and this concentration was normalized by treatment with chitosan. SOD showed increased activity in liver after 15 and 30 days of exposure, 30% and 36%, respectively, and in fish exposed to RCM there was no change in this activity compared with the control group. Increased CAT activity in liver was observed during all experimental periods in fish exposed to CMW (46%, 50%, and 56% at 7, 15, and 30 days, respectively) compared with the control or treated-water groups. The highest increase in hepatic GST activity (106%) was observed only in fish exposed to CMW for 30 days. There was an increase in DNA damage in liver (50% at 7 and 15 days) and blood (79%, 77%, and 48% at 7, 15, and 30 days, respectively) after exposure to CMW. In contrast, the fish exposed to wastewater treated with chitosan microspheres exhibited DNA fragmentation indexes similar to the control group. The results obtained indicate the use of oxidative stress biomarkers as useful tools for the toxicity evaluation of coal mining effluents and also suggest that chitosan microspheres may be used as an alternative approach for remediation of coal mining wastewaters.</description><subject>01 COAL, LIGNITE, AND PEAT</subject><subject>ACID MINE DRAINAGE</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Applied sciences</subject><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MARKERS</subject><subject>Biomarkers</subject><subject>Blood</subject><subject>Catalase - physiology</subject><subject>CHITIN</subject><subject>chitosan</subject><subject>Chitosan - administration & dosage</subject><subject>Cichlids</subject><subject>coal</subject><subject>Coal Mining</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>Drinking water</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effluents</subject><subject>Environmental degradation</subject><subject>Environmental Restoration and Remediation - methods</subject><subject>EVALUATION</subject><subject>Exact sciences and technology</subject><subject>Fish</subject><subject>FISHES</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glutathione - analysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lipid Peroxidation</subject><subject>Liver</subject><subject>Microspheres</subject><subject>Oreochromis niloticus</subject><subject>Oxidative Stress</subject><subject>Pollution</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>REMEDIAL ACTION</subject><subject>Remediation</subject><subject>TOXICITY</subject><subject>Waste Disposal, Fluid - methods</subject><subject>WASTE WATER</subject><subject>Wastewater treatment</subject><subject>Wastewaters</subject><subject>Water monitoring</subject><subject>Water Purification - methods</subject><subject>Water quality</subject><subject>Water quality management</subject><subject>WATER TREATMENT</subject><subject>Water treatment and pollution</subject><issn>0090-4341</issn><issn>1432-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0l2L1DAUBuAgijuO_gBvtCh6Vz0nSdPkUof1A1YEdfBKQppJdrJ2mtmkVfbfm9LBBW-EQAh5zoGTN4Q8RniFAO3rDEA5rwGaGlC2Nb9DVsgZraEFdpesABTUnHE8Iw9yvgJAKiW_T85QKCmlUCvy4_yX6SczhjhU0Vdf3MHtwt_jJpq--hSGMFxW300e3W8zulR1N9VmH8aYzVBubYr5uHfJ5WqbZ_k2xINJP13KD8k9b_rsHp32Ndm-O_-2-VBffH7_cfPmora8hbFmu86j9UJK37ROdb6ljZON66yUjAuPqFrBGJod3UnhjRTUlamEUWBFBx1bk-dL35jHoLMNo7N7G4fB2VFTkFiKRVEvF3VM8XpyedSHkK3rezO4OGVNVdMo1eD_ITRSlJ4FPvsHXsUpDWVU3TKKSFs1I1zQ_FA5Oa-PKZQHutEIes5RLznqkqOec9S81Dw5NZ66kshtxSm4Al6cgMnW9D6ZwYZ86yQvq_yCNXm6OG-iNpepmO1XCsgAEWTTAPsDNAetEQ</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Benassi, J. 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C ; Laus, R ; Geremias, R ; Lima, P. L ; Menezes, C. T. B ; Laranjeira, M. C. M ; Wilhelm-Filho, D ; Fávere, V. T ; Pedrosa, R. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-3dbf1cf688f57e9bf725e85ebc88346f11976331ad2d86fa862e3416a90c6b0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>01 COAL, LIGNITE, AND PEAT</topic><topic>ACID MINE DRAINAGE</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Applied sciences</topic><topic>Bioassays</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MARKERS</topic><topic>Biomarkers</topic><topic>Blood</topic><topic>Catalase - physiology</topic><topic>CHITIN</topic><topic>chitosan</topic><topic>Chitosan - administration & dosage</topic><topic>Cichlids</topic><topic>coal</topic><topic>Coal Mining</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Drinking water</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effluents</topic><topic>Environmental degradation</topic><topic>Environmental Restoration and Remediation - methods</topic><topic>EVALUATION</topic><topic>Exact sciences and technology</topic><topic>Fish</topic><topic>FISHES</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glutathione - analysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lipid Peroxidation</topic><topic>Liver</topic><topic>Microspheres</topic><topic>Oreochromis niloticus</topic><topic>Oxidative Stress</topic><topic>Pollution</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>REMEDIAL ACTION</topic><topic>Remediation</topic><topic>TOXICITY</topic><topic>Waste Disposal, Fluid - methods</topic><topic>WASTE WATER</topic><topic>Wastewater treatment</topic><topic>Wastewaters</topic><topic>Water monitoring</topic><topic>Water Purification - methods</topic><topic>Water quality</topic><topic>Water quality management</topic><topic>WATER TREATMENT</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benassi, J. 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C</au><au>Laus, R</au><au>Geremias, R</au><au>Lima, P. L</au><au>Menezes, C. T. B</au><au>Laranjeira, M. C. M</au><au>Wilhelm-Filho, D</au><au>Fávere, V. T</au><au>Pedrosa, R. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Remediation of Coal Mining Wastewater by Chitosan Microspheres Using Biomarkers</atitle><jtitle>Archives of environmental contamination and toxicology</jtitle><addtitle>Arch Environ Contam Toxicol</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>51</volume><issue>4</issue><spage>633</spage><epage>640</epage><pages>633-640</pages><issn>0090-4341</issn><eissn>1432-0703</eissn><coden>AECTCV</coden><abstract>Acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. Therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. The aim of this work was to evaluate the remediation of mining wastewater effluents by chitosan microspheres using biomarkers of exposure and effect. DNA damage (Comet assay) and several biomarkers of oxidative stress, such as lipoperoxidation levels (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities, and contents of reduced glutathione (GSH), were measured in blood and liver of tilapia (Oreochromis niloticus) exposed for 7, 15, and 30 days to dechlorinated tap water, 10% coal mining wastewater (CMW), and coal mining wastewater treated with chitosan microspheres (RCM). The results indicate that hepatic TBARS levels were significantly higher in fish exposed to CMW after 7, 15, and 30 days (100%, 86%, and 63%, respectively), and after remediation there was no significant difference in relation to the control group. Hepatic GSH concentrations were lower than control values for CMW after 7 and 15 days of exposure (34% decrease at both times), and this concentration was normalized by treatment with chitosan. SOD showed increased activity in liver after 15 and 30 days of exposure, 30% and 36%, respectively, and in fish exposed to RCM there was no change in this activity compared with the control group. Increased CAT activity in liver was observed during all experimental periods in fish exposed to CMW (46%, 50%, and 56% at 7, 15, and 30 days, respectively) compared with the control or treated-water groups. The highest increase in hepatic GST activity (106%) was observed only in fish exposed to CMW for 30 days. There was an increase in DNA damage in liver (50% at 7 and 15 days) and blood (79%, 77%, and 48% at 7, 15, and 30 days, respectively) after exposure to CMW. In contrast, the fish exposed to wastewater treated with chitosan microspheres exhibited DNA fragmentation indexes similar to the control group. The results obtained indicate the use of oxidative stress biomarkers as useful tools for the toxicity evaluation of coal mining effluents and also suggest that chitosan microspheres may be used as an alternative approach for remediation of coal mining wastewaters.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><cop>New York, NY</cop><pub>New York : Springer-Verlag</pub><pmid>16988869</pmid><doi>10.1007/s00244-005-0187-4</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0090-4341 |
| ispartof | Archives of environmental contamination and toxicology, 2006-11, Vol.51 (4), p.633-640 |
| issn | 0090-4341 1432-0703 |
| language | eng |
| recordid | cdi_osti_scitechconnect_20813316 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | 01 COAL, LIGNITE, AND PEAT ACID MINE DRAINAGE Animal, plant and microbial ecology Animals Applied ecology Applied sciences Bioassays Biological and medical sciences BIOLOGICAL MARKERS Biomarkers Blood Catalase - physiology CHITIN chitosan Chitosan - administration & dosage Cichlids coal Coal Mining Deoxyribonucleic acid DNA DNA Damage Drinking water Ecotoxicology, biological effects of pollution Effluents Environmental degradation Environmental Restoration and Remediation - methods EVALUATION Exact sciences and technology Fish FISHES Freshwater Fundamental and applied biological sciences. Psychology General aspects Glutathione - analysis Hydrogen-Ion Concentration Lipid Peroxidation Liver Microspheres Oreochromis niloticus Oxidative Stress Pollution Reactive Oxygen Species - metabolism REMEDIAL ACTION Remediation TOXICITY Waste Disposal, Fluid - methods WASTE WATER Wastewater treatment Wastewaters Water monitoring Water Purification - methods Water quality Water quality management WATER TREATMENT Water treatment and pollution |
| title | Evaluation of Remediation of Coal Mining Wastewater by Chitosan Microspheres Using Biomarkers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T05%3A57%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20Remediation%20of%20Coal%20Mining%20Wastewater%20by%20Chitosan%20Microspheres%20Using%20Biomarkers&rft.jtitle=Archives%20of%20environmental%20contamination%20and%20toxicology&rft.au=Benassi,%20J.%20C&rft.date=2006-11-01&rft.volume=51&rft.issue=4&rft.spage=633&rft.epage=640&rft.pages=633-640&rft.issn=0090-4341&rft.eissn=1432-0703&rft.coden=AECTCV&rft_id=info:doi/10.1007/s00244-005-0187-4&rft_dat=%3Cproquest_osti_%3E20586133%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=732112793&rft_id=info:pmid/16988869&rfr_iscdi=true |