Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells
We examined whether PDGF may directly stimulate the expression of VEGF by retinal pigment epithelial (RPE) cells in vitro, and the involvement of three signal transduction pathways in the regulation of PDGF-evoked cell proliferation, migration, and production of VEGF-A was investigated. PDGF stimula...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-06, Vol.344 (3), p.912-919 |
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| creator | Hollborn, Margrit Bringmann, Andreas Faude, Frank Wiedemann, Peter Kohen, Leon |
| description | We examined whether PDGF may directly stimulate the expression of VEGF by retinal pigment epithelial (RPE) cells in vitro, and the involvement of three signal transduction pathways in the regulation of PDGF-evoked cell proliferation, migration, and production of VEGF-A was investigated. PDGF stimulated the gene and protein expression of VEGF-A by RPE cells, and increased cell proliferation and chemotaxis. PDGF activated all signaling pathways investigated, as determined by increased phosphorylation levels of ERK1/2, p38, and Akt proteins. The three signaling pathways were involved in the mediation of PDGF-evoked cell proliferation, while p38 and PI3K mediated cell migration, and PI3K mediated secretion of VEGF-A. In addition to VEGF-A, the cells expressed mRNAs for various members of the VEGF family and for their receptors, including VEGF-B, -C, -D, flt-1, and KDR. The data indicate that PDGF selectively stimulates the expression of VEGF-A in RPE cells. PDGF evokes at least three signal transduction pathways which are differentially involved in various cellular responses. |
| doi_str_mv | 10.1016/j.bbrc.2006.03.185 |
| format | Article |
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PDGF stimulated the gene and protein expression of VEGF-A by RPE cells, and increased cell proliferation and chemotaxis. PDGF activated all signaling pathways investigated, as determined by increased phosphorylation levels of ERK1/2, p38, and Akt proteins. The three signaling pathways were involved in the mediation of PDGF-evoked cell proliferation, while p38 and PI3K mediated cell migration, and PI3K mediated secretion of VEGF-A. In addition to VEGF-A, the cells expressed mRNAs for various members of the VEGF family and for their receptors, including VEGF-B, -C, -D, flt-1, and KDR. The data indicate that PDGF selectively stimulates the expression of VEGF-A in RPE cells. PDGF evokes at least three signal transduction pathways which are differentially involved in various cellular responses.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.03.185</identifier><identifier>PMID: 16631611</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Cell Movement - drug effects ; CELL PROLIFERATION ; Cell Proliferation - drug effects ; Cells, Cultured ; Chemotaxis ; Dose-Response Relationship, Drug ; ERK1/2 ; GENE REGULATION ; Humans ; IN VITRO ; MAP Kinase Signaling System - drug effects ; MAP Kinase Signaling System - physiology ; p38 MAPK ; PDGF stimulation ; PHOSPHORYLATION ; PI3K ; Pigment Epithelium of Eye - cytology ; Pigment Epithelium of Eye - drug effects ; Pigment Epithelium of Eye - physiology ; Platelet-Derived Growth Factor - administration & dosage ; Proliferation ; RECEPTORS ; Retinal pigment epithelial cells ; RHODOPSIN ; SECRETION ; Signal Transduction - drug effects ; Signal Transduction - physiology ; STIMULATION ; Vascular Endothelial Growth Factor A - metabolism ; VEGF expression</subject><ispartof>Biochemical and biophysical research communications, 2006-06, Vol.344 (3), p.912-919</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-138f618f2de79429069038c2bb2d55ba425a4cd9953dfd68f5da567a7b81081f3</citedby><cites>FETCH-LOGICAL-c413t-138f618f2de79429069038c2bb2d55ba425a4cd9953dfd68f5da567a7b81081f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X06007480$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16631611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20798993$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Hollborn, Margrit</creatorcontrib><creatorcontrib>Bringmann, Andreas</creatorcontrib><creatorcontrib>Faude, Frank</creatorcontrib><creatorcontrib>Wiedemann, Peter</creatorcontrib><creatorcontrib>Kohen, Leon</creatorcontrib><title>Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We examined whether PDGF may directly stimulate the expression of VEGF by retinal pigment epithelial (RPE) cells in vitro, and the involvement of three signal transduction pathways in the regulation of PDGF-evoked cell proliferation, migration, and production of VEGF-A was investigated. PDGF stimulated the gene and protein expression of VEGF-A by RPE cells, and increased cell proliferation and chemotaxis. PDGF activated all signaling pathways investigated, as determined by increased phosphorylation levels of ERK1/2, p38, and Akt proteins. The three signaling pathways were involved in the mediation of PDGF-evoked cell proliferation, while p38 and PI3K mediated cell migration, and PI3K mediated secretion of VEGF-A. In addition to VEGF-A, the cells expressed mRNAs for various members of the VEGF family and for their receptors, including VEGF-B, -C, -D, flt-1, and KDR. The data indicate that PDGF selectively stimulates the expression of VEGF-A in RPE cells. PDGF evokes at least three signal transduction pathways which are differentially involved in various cellular responses.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Cell Movement - drug effects</subject><subject>CELL PROLIFERATION</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Chemotaxis</subject><subject>Dose-Response Relationship, Drug</subject><subject>ERK1/2</subject><subject>GENE REGULATION</subject><subject>Humans</subject><subject>IN VITRO</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>MAP Kinase Signaling System - physiology</subject><subject>p38 MAPK</subject><subject>PDGF stimulation</subject><subject>PHOSPHORYLATION</subject><subject>PI3K</subject><subject>Pigment Epithelium of Eye - cytology</subject><subject>Pigment Epithelium of Eye - drug effects</subject><subject>Pigment Epithelium of Eye - physiology</subject><subject>Platelet-Derived Growth Factor - administration & dosage</subject><subject>Proliferation</subject><subject>RECEPTORS</subject><subject>Retinal pigment epithelial cells</subject><subject>RHODOPSIN</subject><subject>SECRETION</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><subject>STIMULATION</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF expression</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhD3BAkZC4JczYiRNLXFBpC1IlVnxI3CzHdrpesvbWThb139fprsQNTrY1z7ya8UPIa4QKAfn7bdX3UVcUgFfAKuyaJ2SFIKCkCPVTsoJcKanAX2fkRUpbAMSai-fkDDlnyBFXZP3d3Xo1On9b7NW0-aPuU-H8IYwHa_KlWH-6virtIfzOT23HcR5VLKJN--CTXdBiM--UL76tLx_r6SV5Nqgx2Ven85z8vLr8cfG5vPl6_eXi402pa2RTiawbOHYDNbYVNRXABbBO076npml6VdNG1doI0TAzGN4NjVENb1XbdwgdDuycvD3mhjQ5mbSbrN7o4L3Vk6TQik4Ilql3R2ofw91s0yR3Li1zKm_DnCRvRWY5_BfEFltKeZtBegR1DClFO8h9dDsV7yWCXLTIrVy0yEWLBCazltz05pQ-9ztr_racPGTgwxGw-csOzsZlI-u1NS4uC5ng_pX_AFfBnI4</recordid><startdate>20060609</startdate><enddate>20060609</enddate><creator>Hollborn, Margrit</creator><creator>Bringmann, Andreas</creator><creator>Faude, Frank</creator><creator>Wiedemann, Peter</creator><creator>Kohen, Leon</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20060609</creationdate><title>Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells</title><author>Hollborn, Margrit ; Bringmann, Andreas ; Faude, Frank ; Wiedemann, Peter ; Kohen, Leon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-138f618f2de79429069038c2bb2d55ba425a4cd9953dfd68f5da567a7b81081f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Cell Movement - drug effects</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Chemotaxis</topic><topic>Dose-Response Relationship, Drug</topic><topic>ERK1/2</topic><topic>GENE REGULATION</topic><topic>Humans</topic><topic>IN VITRO</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>MAP Kinase Signaling System - physiology</topic><topic>p38 MAPK</topic><topic>PDGF stimulation</topic><topic>PHOSPHORYLATION</topic><topic>PI3K</topic><topic>Pigment Epithelium of Eye - cytology</topic><topic>Pigment Epithelium of Eye - drug effects</topic><topic>Pigment Epithelium of Eye - physiology</topic><topic>Platelet-Derived Growth Factor - administration & dosage</topic><topic>Proliferation</topic><topic>RECEPTORS</topic><topic>Retinal pigment epithelial cells</topic><topic>RHODOPSIN</topic><topic>SECRETION</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - physiology</topic><topic>STIMULATION</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>VEGF expression</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hollborn, Margrit</creatorcontrib><creatorcontrib>Bringmann, Andreas</creatorcontrib><creatorcontrib>Faude, Frank</creatorcontrib><creatorcontrib>Wiedemann, Peter</creatorcontrib><creatorcontrib>Kohen, Leon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hollborn, Margrit</au><au>Bringmann, Andreas</au><au>Faude, Frank</au><au>Wiedemann, Peter</au><au>Kohen, Leon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-06-09</date><risdate>2006</risdate><volume>344</volume><issue>3</issue><spage>912</spage><epage>919</epage><pages>912-919</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We examined whether PDGF may directly stimulate the expression of VEGF by retinal pigment epithelial (RPE) cells in vitro, and the involvement of three signal transduction pathways in the regulation of PDGF-evoked cell proliferation, migration, and production of VEGF-A was investigated. PDGF stimulated the gene and protein expression of VEGF-A by RPE cells, and increased cell proliferation and chemotaxis. PDGF activated all signaling pathways investigated, as determined by increased phosphorylation levels of ERK1/2, p38, and Akt proteins. The three signaling pathways were involved in the mediation of PDGF-evoked cell proliferation, while p38 and PI3K mediated cell migration, and PI3K mediated secretion of VEGF-A. In addition to VEGF-A, the cells expressed mRNAs for various members of the VEGF family and for their receptors, including VEGF-B, -C, -D, flt-1, and KDR. The data indicate that PDGF selectively stimulates the expression of VEGF-A in RPE cells. PDGF evokes at least three signal transduction pathways which are differentially involved in various cellular responses.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16631611</pmid><doi>10.1016/j.bbrc.2006.03.185</doi><tpages>8</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Cell Movement - drug effects CELL PROLIFERATION Cell Proliferation - drug effects Cells, Cultured Chemotaxis Dose-Response Relationship, Drug ERK1/2 GENE REGULATION Humans IN VITRO MAP Kinase Signaling System - drug effects MAP Kinase Signaling System - physiology p38 MAPK PDGF stimulation PHOSPHORYLATION PI3K Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - drug effects Pigment Epithelium of Eye - physiology Platelet-Derived Growth Factor - administration & dosage Proliferation RECEPTORS Retinal pigment epithelial cells RHODOPSIN SECRETION Signal Transduction - drug effects Signal Transduction - physiology STIMULATION Vascular Endothelial Growth Factor A - metabolism VEGF expression |
| title | Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells |
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