Transplantation and differentiation of donor cells in the cloned pigs
The application of nuclear transfer technology is an interesting approach to investigate stem and progenitor cell transplantation therapy. If stem cells are used as a nuclear donor, donor cells can engraft into cloned animals without histocompatible problems. However, it is still uncertain whether d...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-06, Vol.344 (2), p.455-462 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Shimada, Arata Tomii, Ryo Kano, Koichiro Nagashima, Hiroshi |
| description | The application of nuclear transfer technology is an interesting approach to investigate stem and progenitor cell transplantation therapy. If stem cells are used as a nuclear donor, donor cells can engraft into cloned animals without histocompatible problems. However, it is still uncertain whether donor cells can engraft to cloned animal and differentiate in vivo. To address this problem, we transplanted donor cells to dermal tissues of cloned pigs developed by using preadipocytes as donor cells. Preadipocytes are adipocytic progenitor which can differentiate to mature adipocytes in vitro. We showed that the donor preadipocytes were successfully transplanted into the cloned pigs without immune rejection and they differentiated into mature adipocytes in vivo 3 weeks after transplantation. In contrast, allogenic control preadipocytes, which can differentiate in vitro, did not differentiate in vivo. These results indicate that donor progenitor cells can differentiate in cloned animal. |
| doi_str_mv | 10.1016/j.bbrc.2006.03.148 |
| format | Article |
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If stem cells are used as a nuclear donor, donor cells can engraft into cloned animals without histocompatible problems. However, it is still uncertain whether donor cells can engraft to cloned animal and differentiate in vivo. To address this problem, we transplanted donor cells to dermal tissues of cloned pigs developed by using preadipocytes as donor cells. Preadipocytes are adipocytic progenitor which can differentiate to mature adipocytes in vitro. We showed that the donor preadipocytes were successfully transplanted into the cloned pigs without immune rejection and they differentiated into mature adipocytes in vivo 3 weeks after transplantation. In contrast, allogenic control preadipocytes, which can differentiate in vitro, did not differentiate in vivo. 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| subjects | 60 APPLIED LIFE SCIENCES Adipocytes - cytology Adipocytes - transplantation Animals Cell Differentiation Cell transplantation Cells, Cultured Clone animal Cloning, Organism - methods Donor cell IN VITRO IN VIVO In vivo differentiation Multipotent Stem Cells - cytology Multipotent Stem Cells - transplantation Nuclear Transfer Techniques Porcine Preadipocyte STEM CELLS SWINE THERAPY |
| title | Transplantation and differentiation of donor cells in the cloned pigs |
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