Adult rat bone marrow stromal cells express genes associated with dopamine neurons
An intensive search is underway to identify candidates to replace the cells that degenerate in Parkinson’s disease (PD). To date, no suitable substitute has been found. We have recently found that adult rat bone marrow stromal cells (MSCs) can be induced to assume a neuronal phenotype in vitro. Thes...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-05, Vol.343 (4), p.1045-1052 |
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| description | An intensive search is underway to identify candidates to replace the cells that degenerate in Parkinson’s disease (PD). To date, no suitable substitute has been found. We have recently found that adult rat bone marrow stromal cells (MSCs) can be induced to assume a neuronal phenotype in vitro. These findings may have particular relevance to the treatment of PD. We now report that adult MSCs express multiple dopaminergic genes, suggesting that they are potential candidates for cell therapy. Using RT-PCR, we have examined families of genes that are associated with the development and/or survival of dopaminergic neurons. MSCs transcribe a variety of dopaminergic genes including
patched and
smoothened (components of the Shh receptor),
Gli-1 (downstream mediator of Shh), and
Otx-
1, a gene associated with formation of the mesencephalon during development. Furthermore, Shh treatment elicits a 1.5-fold increase in DNA synthesis in cultured MSCs, suggesting the presence of a functional Shh receptor complex. We have also found that MSCs transcribe and translate Nurr-1, a nuclear receptor essential for the development of dopamine neurons. In addition, MSCs express a variety of growth factor receptors including the glycosyl-phosphatidylinositol-anchored ligand-binding subunit of the GDNF receptor,
GFRα1, as well as fibroblast growth factor receptors one and four. The expression of genes that are associated with the development and survival of dopamine neurons suggests a potential role for these cells in the treatment of Parkinson’s disease. |
| doi_str_mv | 10.1016/j.bbrc.2006.02.191 |
| format | Article |
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patched and
smoothened (components of the Shh receptor),
Gli-1 (downstream mediator of Shh), and
Otx-
1, a gene associated with formation of the mesencephalon during development. Furthermore, Shh treatment elicits a 1.5-fold increase in DNA synthesis in cultured MSCs, suggesting the presence of a functional Shh receptor complex. We have also found that MSCs transcribe and translate Nurr-1, a nuclear receptor essential for the development of dopamine neurons. In addition, MSCs express a variety of growth factor receptors including the glycosyl-phosphatidylinositol-anchored ligand-binding subunit of the GDNF receptor,
GFRα1, as well as fibroblast growth factor receptors one and four. The expression of genes that are associated with the development and survival of dopamine neurons suggests a potential role for these cells in the treatment of Parkinson’s disease.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.02.191</identifier><identifier>PMID: 16574067</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; BIOSYNTHESIS ; BONE MARROW ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; Cells, Cultured ; DNA ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; DOPAMINE ; Dopamine - genetics ; Dopamine - metabolism ; FIBROBLASTS ; GDNF ; GENES ; Glial Cell Line-Derived Neurotrophic Factor Receptors - genetics ; Glial Cell Line-Derived Neurotrophic Factor Receptors - metabolism ; GROWTH FACTORS ; IN VITRO ; Kruppel-Like Transcription Factors - genetics ; Kruppel-Like Transcription Factors - metabolism ; LIGANDS ; NERVE CELLS ; NERVOUS SYSTEM DISEASES ; Neurons - cytology ; Neurons - metabolism ; Nuclear Receptor Subfamily 4, Group A, Member 2 ; Nurr-1 ; Otx Transcription Factors - genetics ; Otx Transcription Factors - metabolism ; Parkinson’s disease ; Patched Receptors ; PHENOTYPE ; POLYMERASE CHAIN REACTION ; RATS ; RECEPTORS ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Regenerative medicine ; Reverse Transcriptase Polymerase Chain Reaction ; Smoothened Receptor ; Stem cell ; STEM CELLS ; Stromal Cells - cytology ; Stromal Cells - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Zinc Finger Protein GLI1</subject><ispartof>Biochemical and biophysical research communications, 2006-05, Vol.343 (4), p.1045-1052</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-c5f053a81806da6ff40991894473048a7e98f4f23deb9d3f25182a832aefa5493</citedby><cites>FETCH-LOGICAL-c413t-c5f053a81806da6ff40991894473048a7e98f4f23deb9d3f25182a832aefa5493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2006.02.191$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16574067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20798950$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kramer, Brian C.</creatorcontrib><creatorcontrib>Woodbury, Dale</creatorcontrib><creatorcontrib>Black, Ira B.</creatorcontrib><title>Adult rat bone marrow stromal cells express genes associated with dopamine neurons</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>An intensive search is underway to identify candidates to replace the cells that degenerate in Parkinson’s disease (PD). To date, no suitable substitute has been found. We have recently found that adult rat bone marrow stromal cells (MSCs) can be induced to assume a neuronal phenotype in vitro. These findings may have particular relevance to the treatment of PD. We now report that adult MSCs express multiple dopaminergic genes, suggesting that they are potential candidates for cell therapy. Using RT-PCR, we have examined families of genes that are associated with the development and/or survival of dopaminergic neurons. MSCs transcribe a variety of dopaminergic genes including
patched and
smoothened (components of the Shh receptor),
Gli-1 (downstream mediator of Shh), and
Otx-
1, a gene associated with formation of the mesencephalon during development. Furthermore, Shh treatment elicits a 1.5-fold increase in DNA synthesis in cultured MSCs, suggesting the presence of a functional Shh receptor complex. We have also found that MSCs transcribe and translate Nurr-1, a nuclear receptor essential for the development of dopamine neurons. In addition, MSCs express a variety of growth factor receptors including the glycosyl-phosphatidylinositol-anchored ligand-binding subunit of the GDNF receptor,
GFRα1, as well as fibroblast growth factor receptors one and four. The expression of genes that are associated with the development and survival of dopamine neurons suggests a potential role for these cells in the treatment of Parkinson’s disease.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>BIOSYNTHESIS</subject><subject>BONE MARROW</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cells, Cultured</subject><subject>DNA</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DOPAMINE</subject><subject>Dopamine - genetics</subject><subject>Dopamine - metabolism</subject><subject>FIBROBLASTS</subject><subject>GDNF</subject><subject>GENES</subject><subject>Glial Cell Line-Derived Neurotrophic Factor Receptors - genetics</subject><subject>Glial Cell Line-Derived Neurotrophic Factor Receptors - metabolism</subject><subject>GROWTH FACTORS</subject><subject>IN VITRO</subject><subject>Kruppel-Like Transcription Factors - genetics</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>LIGANDS</subject><subject>NERVE CELLS</subject><subject>NERVOUS SYSTEM DISEASES</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Nuclear Receptor Subfamily 4, Group A, Member 2</subject><subject>Nurr-1</subject><subject>Otx Transcription Factors - genetics</subject><subject>Otx Transcription Factors - metabolism</subject><subject>Parkinson’s disease</subject><subject>Patched Receptors</subject><subject>PHENOTYPE</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>RATS</subject><subject>RECEPTORS</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Regenerative medicine</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Smoothened Receptor</subject><subject>Stem cell</subject><subject>STEM CELLS</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Zinc Finger Protein GLI1</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYO4uOPoF_AgAWFv3Val038CXpbFVWFBEAVvIZ2udjN0d8Yk7a7f3jQz4E0PRR3q9x68eoy9QigRsHl7KPs-2FIANCWIEhU-YTsEBYVAkE_ZDvKlEAq_X7LnMR4AEGWjnrFLbOpWQtPu2JfrYZ0SDybx3i_EZxOCf-AxBT-biVuapsjp8RgoRv6DForcxOitM4kG_uDSPR_80cwuaxdag1_iC3YxminSy_Pes2-377_efCzuPn_4dHN9V1iJVSpsPUJdmQ47aAbTjKMEpbBTUrYVyM60pLpRjqIaqFdDNYoaO2G6ShgaTS1VtWdvTr4-JqejdYnsvfXLQjZpAa3qVA2ZujpRx-B_rhSTnl3cYpmF_Bp10yoQjfg_iC12FebZM3ECbfAxBhr1Mbj8t98aQW_F6IPeitFbMRqEzsVk0euz-9rPNPyVnJvIwLsTQPllvxyFLREtlgYXtkCDd__y_wMrGp4r</recordid><startdate>20060519</startdate><enddate>20060519</enddate><creator>Kramer, Brian C.</creator><creator>Woodbury, Dale</creator><creator>Black, Ira B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20060519</creationdate><title>Adult rat bone marrow stromal cells express genes associated with dopamine neurons</title><author>Kramer, Brian C. ; Woodbury, Dale ; Black, Ira B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-c5f053a81806da6ff40991894473048a7e98f4f23deb9d3f25182a832aefa5493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>BIOSYNTHESIS</topic><topic>BONE MARROW</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cells, Cultured</topic><topic>DNA</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DOPAMINE</topic><topic>Dopamine - genetics</topic><topic>Dopamine - metabolism</topic><topic>FIBROBLASTS</topic><topic>GDNF</topic><topic>GENES</topic><topic>Glial Cell Line-Derived Neurotrophic Factor Receptors - genetics</topic><topic>Glial Cell Line-Derived Neurotrophic Factor Receptors - metabolism</topic><topic>GROWTH FACTORS</topic><topic>IN VITRO</topic><topic>Kruppel-Like Transcription Factors - genetics</topic><topic>Kruppel-Like Transcription Factors - metabolism</topic><topic>LIGANDS</topic><topic>NERVE CELLS</topic><topic>NERVOUS SYSTEM DISEASES</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Nuclear Receptor Subfamily 4, Group A, Member 2</topic><topic>Nurr-1</topic><topic>Otx Transcription Factors - genetics</topic><topic>Otx Transcription Factors - metabolism</topic><topic>Parkinson’s disease</topic><topic>Patched Receptors</topic><topic>PHENOTYPE</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>RATS</topic><topic>RECEPTORS</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Regenerative medicine</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Smoothened Receptor</topic><topic>Stem cell</topic><topic>STEM CELLS</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Zinc Finger Protein GLI1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kramer, Brian C.</creatorcontrib><creatorcontrib>Woodbury, Dale</creatorcontrib><creatorcontrib>Black, Ira B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kramer, Brian C.</au><au>Woodbury, Dale</au><au>Black, Ira B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adult rat bone marrow stromal cells express genes associated with dopamine neurons</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-05-19</date><risdate>2006</risdate><volume>343</volume><issue>4</issue><spage>1045</spage><epage>1052</epage><pages>1045-1052</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>An intensive search is underway to identify candidates to replace the cells that degenerate in Parkinson’s disease (PD). To date, no suitable substitute has been found. We have recently found that adult rat bone marrow stromal cells (MSCs) can be induced to assume a neuronal phenotype in vitro. These findings may have particular relevance to the treatment of PD. We now report that adult MSCs express multiple dopaminergic genes, suggesting that they are potential candidates for cell therapy. Using RT-PCR, we have examined families of genes that are associated with the development and/or survival of dopaminergic neurons. MSCs transcribe a variety of dopaminergic genes including
patched and
smoothened (components of the Shh receptor),
Gli-1 (downstream mediator of Shh), and
Otx-
1, a gene associated with formation of the mesencephalon during development. Furthermore, Shh treatment elicits a 1.5-fold increase in DNA synthesis in cultured MSCs, suggesting the presence of a functional Shh receptor complex. We have also found that MSCs transcribe and translate Nurr-1, a nuclear receptor essential for the development of dopamine neurons. In addition, MSCs express a variety of growth factor receptors including the glycosyl-phosphatidylinositol-anchored ligand-binding subunit of the GDNF receptor,
GFRα1, as well as fibroblast growth factor receptors one and four. The expression of genes that are associated with the development and survival of dopamine neurons suggests a potential role for these cells in the treatment of Parkinson’s disease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16574067</pmid><doi>10.1016/j.bbrc.2006.02.191</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2006-05, Vol.343 (4), p.1045-1052 |
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| subjects | 60 APPLIED LIFE SCIENCES Animals BIOSYNTHESIS BONE MARROW Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cells, Cultured DNA DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism DOPAMINE Dopamine - genetics Dopamine - metabolism FIBROBLASTS GDNF GENES Glial Cell Line-Derived Neurotrophic Factor Receptors - genetics Glial Cell Line-Derived Neurotrophic Factor Receptors - metabolism GROWTH FACTORS IN VITRO Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism LIGANDS NERVE CELLS NERVOUS SYSTEM DISEASES Neurons - cytology Neurons - metabolism Nuclear Receptor Subfamily 4, Group A, Member 2 Nurr-1 Otx Transcription Factors - genetics Otx Transcription Factors - metabolism Parkinson’s disease Patched Receptors PHENOTYPE POLYMERASE CHAIN REACTION RATS RECEPTORS Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Regenerative medicine Reverse Transcriptase Polymerase Chain Reaction Smoothened Receptor Stem cell STEM CELLS Stromal Cells - cytology Stromal Cells - metabolism Transcription Factors - genetics Transcription Factors - metabolism Zinc Finger Protein GLI1 |
| title | Adult rat bone marrow stromal cells express genes associated with dopamine neurons |
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