Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands
Salivary gland stem/progenitor cells are thought to be present in intercalated ductal cells, but the fact is unclear. In this study, we sought to clarify if stem/progenitor cells are present in submandibular glands using colony assay, which is one of the stem cell assay methods. Using a low-density...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-02, Vol.340 (2), p.544-552 |
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| creator | Kishi, Teruki Takao, Tukasa Fujita, Kiyohide Taniguchi, Hideki |
| description | Salivary gland stem/progenitor cells are thought to be present in intercalated ductal cells, but the fact is unclear. In this study, we sought to clarify if stem/progenitor cells are present in submandibular glands using colony assay, which is one of the stem cell assay methods. Using a low-density culture of submandibular gland cells of neonatal rats, we developed a novel culture system that promotes single cell colony formation. Average doubling time for the colony-forming cells was 24.7 (SD
=
±7.02)
h, indicating high proliferative potency. When epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were added to the medium, the number of clonal colonies increased greater than those cultured without growth factors (13.2
±
4.18 vs. 4.5
±
1.73). The RT-PCR and immunostaining demonstrated expressing acinar, ductal, and myoepithelial cell lineage markers. This study demonstrated the presence of the salivary gland stem/progenitor cells that are highly proliferative and multipotent in salivary glands. |
| doi_str_mv | 10.1016/j.bbrc.2005.12.031 |
| format | Article |
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=
±7.02)
h, indicating high proliferative potency. When epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were added to the medium, the number of clonal colonies increased greater than those cultured without growth factors (13.2
±
4.18 vs. 4.5
±
1.73). The RT-PCR and immunostaining demonstrated expressing acinar, ductal, and myoepithelial cell lineage markers. This study demonstrated the presence of the salivary gland stem/progenitor cells that are highly proliferative and multipotent in salivary glands.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.12.031</identifier><identifier>PMID: 16376857</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; Animals, Newborn - growth & development ; Cell Culture Techniques ; CELL PROLIFERATION ; Cells, Cultured ; Clone Cells ; Colony assay ; COLONY FORMATION ; Colony-Forming Units Assay ; Ductal cell ; EGF ; Genetic Markers ; GROWTH FACTORS ; HGF ; High proliferation ; Immunohistochemistry ; Multipotency ; Multipotent Stem Cells - cytology ; Multipotent Stem Cells - physiology ; POLYMERASE CHAIN REACTION ; Rat ; RATS ; Rats, Wistar ; Salivary gland ; SALIVARY GLANDS ; Single cell colony ; STEM CELLS ; Stem/progenitor cell ; Submandibular Gland - cytology ; Submandibular Gland - growth & development ; Submandibular Gland - physiology</subject><ispartof>Biochemical and biophysical research communications, 2006-02, Vol.340 (2), p.544-552</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-1322d96802fff9473f60501dbb35b56b88929cb484342e5a2657ce50a591f3443</citedby><cites>FETCH-LOGICAL-c479t-1322d96802fff9473f60501dbb35b56b88929cb484342e5a2657ce50a591f3443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2005.12.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16376857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20798794$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kishi, Teruki</creatorcontrib><creatorcontrib>Takao, Tukasa</creatorcontrib><creatorcontrib>Fujita, Kiyohide</creatorcontrib><creatorcontrib>Taniguchi, Hideki</creatorcontrib><title>Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Salivary gland stem/progenitor cells are thought to be present in intercalated ductal cells, but the fact is unclear. In this study, we sought to clarify if stem/progenitor cells are present in submandibular glands using colony assay, which is one of the stem cell assay methods. Using a low-density culture of submandibular gland cells of neonatal rats, we developed a novel culture system that promotes single cell colony formation. Average doubling time for the colony-forming cells was 24.7 (SD
=
±7.02)
h, indicating high proliferative potency. When epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were added to the medium, the number of clonal colonies increased greater than those cultured without growth factors (13.2
±
4.18 vs. 4.5
±
1.73). The RT-PCR and immunostaining demonstrated expressing acinar, ductal, and myoepithelial cell lineage markers. This study demonstrated the presence of the salivary gland stem/progenitor cells that are highly proliferative and multipotent in salivary glands.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>Animals, Newborn - growth & development</subject><subject>Cell Culture Techniques</subject><subject>CELL PROLIFERATION</subject><subject>Cells, Cultured</subject><subject>Clone Cells</subject><subject>Colony assay</subject><subject>COLONY FORMATION</subject><subject>Colony-Forming Units Assay</subject><subject>Ductal cell</subject><subject>EGF</subject><subject>Genetic Markers</subject><subject>GROWTH FACTORS</subject><subject>HGF</subject><subject>High proliferation</subject><subject>Immunohistochemistry</subject><subject>Multipotency</subject><subject>Multipotent Stem Cells - cytology</subject><subject>Multipotent Stem Cells - physiology</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Rat</subject><subject>RATS</subject><subject>Rats, Wistar</subject><subject>Salivary gland</subject><subject>SALIVARY GLANDS</subject><subject>Single cell colony</subject><subject>STEM CELLS</subject><subject>Stem/progenitor cell</subject><subject>Submandibular Gland - cytology</subject><subject>Submandibular Gland - growth & development</subject><subject>Submandibular Gland - physiology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGL1DAYhoMo7uzqH_AgAcFbu1_SJG3AiwzqCgteFLyFNP26m6FNxiSz4L83ZQa86Snw8bwvPHkJecOgZcDU7aEdx-RaDiBbxlvo2DOyY6Ch4QzEc7IDANVwzX5ekeucDwCMCaVfkiumul4Nst8Rt19isAs9prj4GZMtPgYaZ7qeluKPsWAoNBdcbyvxgMGXmKjDZcnUB1oekQasBaVW2DBRO9UYzXbxTzb9pg9LPeZX5MVsl4yvL-8N-fH50_f9XXP_7cvX_cf7xolel4Z1nE9aDcDnedai72YFEtg0jp0cpRqHQXPtRjGITnCUlivZO5RgpWZzJ0R3Q96de2Mu3mTnC7pHF0NAVwyHXg-93qj3Z6oK_TphLmb1eTOy1eSUjeoVCCaG_4JMCy466CvIz6BLMeeEszkmv1Z_w8BsS5mD2ZYy21KGcVOXqqG3l_bTuOL0N3KZpgIfzgDWL3vymDYjDA4nnzahKfp_9f8BouqkOg</recordid><startdate>20060210</startdate><enddate>20060210</enddate><creator>Kishi, Teruki</creator><creator>Takao, Tukasa</creator><creator>Fujita, Kiyohide</creator><creator>Taniguchi, Hideki</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20060210</creationdate><title>Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands</title><author>Kishi, Teruki ; Takao, Tukasa ; Fujita, Kiyohide ; Taniguchi, Hideki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-1322d96802fff9473f60501dbb35b56b88929cb484342e5a2657ce50a591f3443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>Animals, Newborn - growth & development</topic><topic>Cell Culture Techniques</topic><topic>CELL PROLIFERATION</topic><topic>Cells, Cultured</topic><topic>Clone Cells</topic><topic>Colony assay</topic><topic>COLONY FORMATION</topic><topic>Colony-Forming Units Assay</topic><topic>Ductal cell</topic><topic>EGF</topic><topic>Genetic Markers</topic><topic>GROWTH FACTORS</topic><topic>HGF</topic><topic>High proliferation</topic><topic>Immunohistochemistry</topic><topic>Multipotency</topic><topic>Multipotent Stem Cells - cytology</topic><topic>Multipotent Stem Cells - physiology</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Rat</topic><topic>RATS</topic><topic>Rats, Wistar</topic><topic>Salivary gland</topic><topic>SALIVARY GLANDS</topic><topic>Single cell colony</topic><topic>STEM CELLS</topic><topic>Stem/progenitor cell</topic><topic>Submandibular Gland - cytology</topic><topic>Submandibular Gland - growth & development</topic><topic>Submandibular Gland - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishi, Teruki</creatorcontrib><creatorcontrib>Takao, Tukasa</creatorcontrib><creatorcontrib>Fujita, Kiyohide</creatorcontrib><creatorcontrib>Taniguchi, Hideki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishi, Teruki</au><au>Takao, Tukasa</au><au>Fujita, Kiyohide</au><au>Taniguchi, Hideki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-02-10</date><risdate>2006</risdate><volume>340</volume><issue>2</issue><spage>544</spage><epage>552</epage><pages>544-552</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Salivary gland stem/progenitor cells are thought to be present in intercalated ductal cells, but the fact is unclear. In this study, we sought to clarify if stem/progenitor cells are present in submandibular glands using colony assay, which is one of the stem cell assay methods. Using a low-density culture of submandibular gland cells of neonatal rats, we developed a novel culture system that promotes single cell colony formation. Average doubling time for the colony-forming cells was 24.7 (SD
=
±7.02)
h, indicating high proliferative potency. When epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were added to the medium, the number of clonal colonies increased greater than those cultured without growth factors (13.2
±
4.18 vs. 4.5
±
1.73). The RT-PCR and immunostaining demonstrated expressing acinar, ductal, and myoepithelial cell lineage markers. This study demonstrated the presence of the salivary gland stem/progenitor cells that are highly proliferative and multipotent in salivary glands.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16376857</pmid><doi>10.1016/j.bbrc.2005.12.031</doi><tpages>9</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Animals Animals, Newborn - growth & development Cell Culture Techniques CELL PROLIFERATION Cells, Cultured Clone Cells Colony assay COLONY FORMATION Colony-Forming Units Assay Ductal cell EGF Genetic Markers GROWTH FACTORS HGF High proliferation Immunohistochemistry Multipotency Multipotent Stem Cells - cytology Multipotent Stem Cells - physiology POLYMERASE CHAIN REACTION Rat RATS Rats, Wistar Salivary gland SALIVARY GLANDS Single cell colony STEM CELLS Stem/progenitor cell Submandibular Gland - cytology Submandibular Gland - growth & development Submandibular Gland - physiology |
| title | Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands |
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