Rebamipide inhibits gastric cancer growth by targeting survivin and Aurora-B
Rebamipide accelerates healing of gastric ulcers and gastritis but its actions on gastric cancer are not known. Survivin, an anti-apoptosis protein, is overexpressed in stem, progenitor, and cancer cells. In gastric cancer, increased and sustained survivin expression provides survival advantage and...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-08, Vol.334 (1), p.207-212 |
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| creator | Tarnawski, A. Pai, R. Chiou, S.-K. Chai, J. Chu, E.C. |
| description | Rebamipide accelerates healing of gastric ulcers and gastritis but its actions on gastric cancer are not known. Survivin, an anti-apoptosis protein, is overexpressed in stem, progenitor, and cancer cells. In gastric cancer, increased and sustained survivin expression provides survival advantage and facilitates tumor progression and resistance to anti-cancer drugs. Aurora-B kinase is essential for chromosome alignment and mitosis progression but surprisingly its role in gastric cancer has not been explored. We examined in human gastric cancer AGS cells: (1) survivin expression, (2) localization of survivin and Aurora-B, (3) cell proliferation, and (4) effects of specific survivin siRNA and/or rebamipide (free radical scavenging drug) on survivin and Aurora-B expression and cell proliferation. Survivin and Aurora-B are strongly expressed in human AGS gastric cancer cells and co-localize during mitosis. Survivin siRNA significantly reduces AGS cell viability. Rebamipide significantly downregulates in AGS cell survivin expression, its association with Aurora-B and cell proliferation. Rebamipide-induced downregulation of survivin is at the transcription level and does not involve ubiquitin–proteasome pathway. |
| doi_str_mv | 10.1016/j.bbrc.2005.05.204 |
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Survivin, an anti-apoptosis protein, is overexpressed in stem, progenitor, and cancer cells. In gastric cancer, increased and sustained survivin expression provides survival advantage and facilitates tumor progression and resistance to anti-cancer drugs. Aurora-B kinase is essential for chromosome alignment and mitosis progression but surprisingly its role in gastric cancer has not been explored. We examined in human gastric cancer AGS cells: (1) survivin expression, (2) localization of survivin and Aurora-B, (3) cell proliferation, and (4) effects of specific survivin siRNA and/or rebamipide (free radical scavenging drug) on survivin and Aurora-B expression and cell proliferation. Survivin and Aurora-B are strongly expressed in human AGS gastric cancer cells and co-localize during mitosis. Survivin siRNA significantly reduces AGS cell viability. Rebamipide significantly downregulates in AGS cell survivin expression, its association with Aurora-B and cell proliferation. Rebamipide-induced downregulation of survivin is at the transcription level and does not involve ubiquitin–proteasome pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.05.204</identifier><identifier>PMID: 15993841</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Alanine - administration & dosage ; Alanine - analogs & derivatives ; APOPTOSIS ; Aurora Kinase B ; Aurora Kinases ; Aurora-B ; Cell Line, Tumor ; CELL PROLIFERATION ; Cell Proliferation - drug effects ; CHROMOSOMES ; Dose-Response Relationship, Drug ; Drug Delivery Systems - methods ; DRUGS ; Gastric cancer ; Gene Expression Regulation, Neoplastic - drug effects ; GROWTH ; HEALING ; Humans ; Inhibitor of Apoptosis Proteins ; Microtubule-Associated Proteins - metabolism ; MITOSIS ; Neoplasm Proteins - metabolism ; NEOPLASMS ; Proteasome inhibitor ; Protein-Serine-Threonine Kinases - antagonists & inhibitors ; Protein-Serine-Threonine Kinases - metabolism ; PROTEINS ; Quinolones - administration & dosage ; Rebamipide ; SCAVENGING ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Survivin ; TRANSCRIPTION ; ULCERS</subject><ispartof>Biochemical and biophysical research communications, 2005-08, Vol.334 (1), p.207-212</ispartof><rights>2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-4b6befbc39223f71a0a81468cf5698bc055765165887804d489149b657e8960d3</citedby><cites>FETCH-LOGICAL-c479t-4b6befbc39223f71a0a81468cf5698bc055765165887804d489149b657e8960d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2005.05.204$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15993841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20710916$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Tarnawski, A.</creatorcontrib><creatorcontrib>Pai, R.</creatorcontrib><creatorcontrib>Chiou, S.-K.</creatorcontrib><creatorcontrib>Chai, J.</creatorcontrib><creatorcontrib>Chu, E.C.</creatorcontrib><title>Rebamipide inhibits gastric cancer growth by targeting survivin and Aurora-B</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Rebamipide accelerates healing of gastric ulcers and gastritis but its actions on gastric cancer are not known. Survivin, an anti-apoptosis protein, is overexpressed in stem, progenitor, and cancer cells. In gastric cancer, increased and sustained survivin expression provides survival advantage and facilitates tumor progression and resistance to anti-cancer drugs. Aurora-B kinase is essential for chromosome alignment and mitosis progression but surprisingly its role in gastric cancer has not been explored. We examined in human gastric cancer AGS cells: (1) survivin expression, (2) localization of survivin and Aurora-B, (3) cell proliferation, and (4) effects of specific survivin siRNA and/or rebamipide (free radical scavenging drug) on survivin and Aurora-B expression and cell proliferation. Survivin and Aurora-B are strongly expressed in human AGS gastric cancer cells and co-localize during mitosis. Survivin siRNA significantly reduces AGS cell viability. Rebamipide significantly downregulates in AGS cell survivin expression, its association with Aurora-B and cell proliferation. Rebamipide-induced downregulation of survivin is at the transcription level and does not involve ubiquitin–proteasome pathway.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Alanine - administration & dosage</subject><subject>Alanine - analogs & derivatives</subject><subject>APOPTOSIS</subject><subject>Aurora Kinase B</subject><subject>Aurora Kinases</subject><subject>Aurora-B</subject><subject>Cell Line, Tumor</subject><subject>CELL PROLIFERATION</subject><subject>Cell Proliferation - drug effects</subject><subject>CHROMOSOMES</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Delivery Systems - methods</subject><subject>DRUGS</subject><subject>Gastric cancer</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>GROWTH</subject><subject>HEALING</subject><subject>Humans</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>MITOSIS</subject><subject>Neoplasm Proteins - metabolism</subject><subject>NEOPLASMS</subject><subject>Proteasome inhibitor</subject><subject>Protein-Serine-Threonine Kinases - antagonists & inhibitors</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>PROTEINS</subject><subject>Quinolones - administration & dosage</subject><subject>Rebamipide</subject><subject>SCAVENGING</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Survivin</subject><subject>TRANSCRIPTION</subject><subject>ULCERS</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFqGzEQhkVpadykL9BDERR6W2e0K2kl6CUNbRMwFEILuQlJO2vL2LuupHXJ20eLDb01MKDLNz-a_yPkA4MlAyavt0vnol_WAGJZpgb-iiwYaKhqBvw1WQCArGrNHi_Iu5S2AIxxqd-SCya0bhRnC7J6QGf34RA6pGHYBBdyomubcgyeejt4jHQdx795Q90TzTauMYdhTdMUj-EYBmqHjt5McYy2-npF3vR2l_D9-b0kv79_-3V7V61-_ri_vVlVnrc6V9xJh73zja7rpm-ZBavKx5TvhdTKeRCilYJJoVSrgHdcaca1k6JFpSV0zSX5dModUw4m-ZDRb_w4DOizqaEtHTBZqM8n6hDHPxOmbPYhedzt7IDjlIxU0JSCXgZZK7hoQRSwPoE-jilF7M0hhr2NT4aBmZWYrZmVmFmJKVOUlKWP5_TJ7bH7t3J2UIAvJwBLZceAcb4IS_NdiPNB3Rj-l_8MiBCawg</recordid><startdate>20050819</startdate><enddate>20050819</enddate><creator>Tarnawski, A.</creator><creator>Pai, R.</creator><creator>Chiou, S.-K.</creator><creator>Chai, J.</creator><creator>Chu, E.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20050819</creationdate><title>Rebamipide inhibits gastric cancer growth by targeting survivin and Aurora-B</title><author>Tarnawski, A. ; Pai, R. ; Chiou, S.-K. ; Chai, J. ; Chu, E.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-4b6befbc39223f71a0a81468cf5698bc055765165887804d489149b657e8960d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Alanine - administration & dosage</topic><topic>Alanine - analogs & derivatives</topic><topic>APOPTOSIS</topic><topic>Aurora Kinase B</topic><topic>Aurora Kinases</topic><topic>Aurora-B</topic><topic>Cell Line, Tumor</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - drug effects</topic><topic>CHROMOSOMES</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Delivery Systems - methods</topic><topic>DRUGS</topic><topic>Gastric cancer</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>GROWTH</topic><topic>HEALING</topic><topic>Humans</topic><topic>Inhibitor of Apoptosis Proteins</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>MITOSIS</topic><topic>Neoplasm Proteins - metabolism</topic><topic>NEOPLASMS</topic><topic>Proteasome inhibitor</topic><topic>Protein-Serine-Threonine Kinases - antagonists & inhibitors</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>PROTEINS</topic><topic>Quinolones - administration & dosage</topic><topic>Rebamipide</topic><topic>SCAVENGING</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Survivin</topic><topic>TRANSCRIPTION</topic><topic>ULCERS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tarnawski, A.</creatorcontrib><creatorcontrib>Pai, R.</creatorcontrib><creatorcontrib>Chiou, S.-K.</creatorcontrib><creatorcontrib>Chai, J.</creatorcontrib><creatorcontrib>Chu, E.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tarnawski, A.</au><au>Pai, R.</au><au>Chiou, S.-K.</au><au>Chai, J.</au><au>Chu, E.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rebamipide inhibits gastric cancer growth by targeting survivin and Aurora-B</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-08-19</date><risdate>2005</risdate><volume>334</volume><issue>1</issue><spage>207</spage><epage>212</epage><pages>207-212</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Rebamipide accelerates healing of gastric ulcers and gastritis but its actions on gastric cancer are not known. Survivin, an anti-apoptosis protein, is overexpressed in stem, progenitor, and cancer cells. In gastric cancer, increased and sustained survivin expression provides survival advantage and facilitates tumor progression and resistance to anti-cancer drugs. Aurora-B kinase is essential for chromosome alignment and mitosis progression but surprisingly its role in gastric cancer has not been explored. We examined in human gastric cancer AGS cells: (1) survivin expression, (2) localization of survivin and Aurora-B, (3) cell proliferation, and (4) effects of specific survivin siRNA and/or rebamipide (free radical scavenging drug) on survivin and Aurora-B expression and cell proliferation. Survivin and Aurora-B are strongly expressed in human AGS gastric cancer cells and co-localize during mitosis. Survivin siRNA significantly reduces AGS cell viability. Rebamipide significantly downregulates in AGS cell survivin expression, its association with Aurora-B and cell proliferation. Rebamipide-induced downregulation of survivin is at the transcription level and does not involve ubiquitin–proteasome pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15993841</pmid><doi>10.1016/j.bbrc.2005.05.204</doi><tpages>6</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Alanine - administration & dosage Alanine - analogs & derivatives APOPTOSIS Aurora Kinase B Aurora Kinases Aurora-B Cell Line, Tumor CELL PROLIFERATION Cell Proliferation - drug effects CHROMOSOMES Dose-Response Relationship, Drug Drug Delivery Systems - methods DRUGS Gastric cancer Gene Expression Regulation, Neoplastic - drug effects GROWTH HEALING Humans Inhibitor of Apoptosis Proteins Microtubule-Associated Proteins - metabolism MITOSIS Neoplasm Proteins - metabolism NEOPLASMS Proteasome inhibitor Protein-Serine-Threonine Kinases - antagonists & inhibitors Protein-Serine-Threonine Kinases - metabolism PROTEINS Quinolones - administration & dosage Rebamipide SCAVENGING Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Survivin TRANSCRIPTION ULCERS |
| title | Rebamipide inhibits gastric cancer growth by targeting survivin and Aurora-B |
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