Intraepithelial lymphocytes express junctional molecules in murine small intestine
Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL e...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-06, Vol.331 (4), p.977-983 |
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| creator | Inagaki-Ohara, Kyoko Sawaguchi, Akira Suganuma, Tatsuo Matsuzaki, Goro Nawa, Yukifumi |
| description | Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), β-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer’s patches did not. γδ IEL showed higher level of these expressions than αβ IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC. |
| doi_str_mv | 10.1016/j.bbrc.2005.04.025 |
| format | Article |
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However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), β-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer’s patches did not. γδ IEL showed higher level of these expressions than αβ IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. 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However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), β-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer’s patches did not. γδ IEL showed higher level of these expressions than αβ IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>CELL PROLIFERATION</subject><subject>DNA Primers</subject><subject>Epithelial cells</subject><subject>Homeostasis</subject><subject>Intestinal intraepithelial lymphocytes</subject><subject>Intestine, Small - cytology</subject><subject>Intestine, Small - metabolism</subject><subject>Intestine, Small - physiology</subject><subject>Junctional Adhesion Molecules</subject><subject>Junctional molecules</subject><subject>LIVER</subject><subject>LYMPH NODES</subject><subject>LYMPHOCYTES</subject><subject>Lymphocytes - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>PROTEINS</subject><subject>SMALL INTESTINE</subject><subject>SPLEEN</subject><subject>THYMUS</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpabZp_0APwVDoze7ow5YNuZTQj0AgEFLoTcjymNUiS45kh-6_r8wu5NaexDDP-86MXkI-Uqgo0ObLoer7aCoGUFcgKmD1K7Kj0EHJKIjXZAcATck6-vuCvEvpAECpaLq35ILWbcs6KXbk4dYvUeNslz06q13hjtO8D-a4YCrwzxwxpeKwerPY4HN7Cg7N6nLT-mJao_VYpEk7l-ssWXL9nrwZtUv44fxekl_fvz3e_Czv7n_c3ny9K42gfCn5SKVsNBc9G3uBRtT1KEba0XoQvNeCasa7RrcSYajbftSsMyPIVjc4tMg5vySfTr4hj1XJ2AXN3gTv0SyKgaQgO5GpzydqjuFpzRuqySaDzmmPYU2qke0Gsv-CVNaCcbbNZSfQxJBSxFHN0U46HhUFtQWjDmoLRm3BKBAqB5NFV2f3tZ9weJGck8jA9QnA_GXPFuN2EXqDg43bQUOw__L_C63Bn9Q</recordid><startdate>20050617</startdate><enddate>20050617</enddate><creator>Inagaki-Ohara, Kyoko</creator><creator>Sawaguchi, Akira</creator><creator>Suganuma, Tatsuo</creator><creator>Matsuzaki, Goro</creator><creator>Nawa, Yukifumi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20050617</creationdate><title>Intraepithelial lymphocytes express junctional molecules in murine small intestine</title><author>Inagaki-Ohara, Kyoko ; Sawaguchi, Akira ; Suganuma, Tatsuo ; Matsuzaki, Goro ; Nawa, Yukifumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-3f1776a34b2fb4ec455f4f1915d43ba41a2396a87e0d58bfa29cf078a6ed8e333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>CELL PROLIFERATION</topic><topic>DNA Primers</topic><topic>Epithelial cells</topic><topic>Homeostasis</topic><topic>Intestinal intraepithelial lymphocytes</topic><topic>Intestine, Small - cytology</topic><topic>Intestine, Small - metabolism</topic><topic>Intestine, Small - physiology</topic><topic>Junctional Adhesion Molecules</topic><topic>Junctional molecules</topic><topic>LIVER</topic><topic>LYMPH NODES</topic><topic>LYMPHOCYTES</topic><topic>Lymphocytes - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>PROTEINS</topic><topic>SMALL INTESTINE</topic><topic>SPLEEN</topic><topic>THYMUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inagaki-Ohara, Kyoko</creatorcontrib><creatorcontrib>Sawaguchi, Akira</creatorcontrib><creatorcontrib>Suganuma, Tatsuo</creatorcontrib><creatorcontrib>Matsuzaki, Goro</creatorcontrib><creatorcontrib>Nawa, Yukifumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inagaki-Ohara, Kyoko</au><au>Sawaguchi, Akira</au><au>Suganuma, Tatsuo</au><au>Matsuzaki, Goro</au><au>Nawa, Yukifumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraepithelial lymphocytes express junctional molecules in murine small intestine</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-06-17</date><risdate>2005</risdate><volume>331</volume><issue>4</issue><spage>977</spage><epage>983</epage><pages>977-983</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), β-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer’s patches did not. γδ IEL showed higher level of these expressions than αβ IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15882974</pmid><doi>10.1016/j.bbrc.2005.04.025</doi><tpages>7</tpages></addata></record> |
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| subjects | 60 APPLIED LIFE SCIENCES Animals Base Sequence Cell Adhesion Molecules - metabolism CELL PROLIFERATION DNA Primers Epithelial cells Homeostasis Intestinal intraepithelial lymphocytes Intestine, Small - cytology Intestine, Small - metabolism Intestine, Small - physiology Junctional Adhesion Molecules Junctional molecules LIVER LYMPH NODES LYMPHOCYTES Lymphocytes - metabolism Mice Mice, Inbred C57BL PROTEINS SMALL INTESTINE SPLEEN THYMUS |
| title | Intraepithelial lymphocytes express junctional molecules in murine small intestine |
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