Efficient killing of CD22 + tumor cells by a humanized diabody–RNase fusion protein

We report on the generation of a dimeric immunoenzyme capable of simultaneously delivering two ribonuclease (RNase) effector domains on one molecule to CD22 + tumor cells. As targeting moiety a diabody derived from the previously humanized scFv SGIII with grafted specificity of the murine anti-CD22...

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Veröffentlicht in:	Biochemical and biophysical research communications 2005-06, Vol.331 (2), p.595-602
Hauptverfasser:	Krauss, Jürgen, Arndt, Michaela A.E., Vu, Bang K., Newton, Dianne L., Seeber, Siegfried, Rybak, Susanna M.
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES AFFINITY Animals ANTIBODIES Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibody Specificity Antigens, CD - immunology Antigens, CD - metabolism Antigens, Differentiation, B-Lymphocyte - immunology Antigens, Differentiation, B-Lymphocyte - metabolism BACTERIA CD22 Cell Adhesion Molecules - immunology Cell Adhesion Molecules - metabolism Cell Death Cell Line, Tumor Cell Survival Cytotoxicity Cytotoxicity, Immunologic Dimerization FROGS Fusion protein Humanized diabody Humans Immunoglobulin Variable Region - chemistry Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Inhibitory Concentration 50 Lectins - immunology Lectins - metabolism LIVER Mice MOLECULES Neoplasms - immunology Neoplasms - pathology Protein Structure, Quaternary Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Ribonuclease Ribonucleases - chemistry Ribonucleases - genetics Ribonucleases - isolation & purification Ribonucleases - metabolism RNA-ASE Sensitivity and Specificity Sialic Acid Binding Ig-like Lectin 2 SPECIFICITY TOXICITY TUMOR CELLS
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