A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response
Reproducing in vitro the complex multiscale physical features of human tissues creates novel biomedical opportunities and fundamental understanding of cell−environment interfaces and interactions. While stiffness has been recognized as a key driver of cell behavior, systematic studies on the role of...
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| Veröffentlicht in: | Advanced healthcare materials 2023-04, Vol.12 (10), p.e2201503-n/a |
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| creator | Zanut, Alessandra Li, Rui Deng, Ru Liu, Xiangyu Rejhon, Martin Chen, Weiqiang Weck, Marcus de Peppo, Giuseppe Maria Riedo, Elisa |
| description | Reproducing in vitro the complex multiscale physical features of human tissues creates novel biomedical opportunities and fundamental understanding of cell−environment interfaces and interactions. While stiffness has been recognized as a key driver of cell behavior, systematic studies on the role of stiffness have been limited to values in the KPa−MPa range, significantly below the stiffness of bone. Here, a platform enabling the tuning of the stiffness of a biocompatible polymeric interface up to values characteristic of human bone is reported, which are in the GPa range, by using extremely thin polymer films on glass and cross‐linking the films using ultraviolet (UV) light irradiation. It is shown that a higher stiffness is related to better adhesion, proliferation, and osteogenic differentiation, and that it is possible to switch on/off cell attachment and growth by solely tuning the stiffness of the interface, without any surface chemistry or topography modification. Since the stiffness is tuned directly by UV irradiation, this platform is ideal for rapid and simple fabrication of stiffness patterns and gradients, thus representing an innovative tool for combinatorial studies of the synergistic effect of tissue environmental cues on cell behavior, and creates new opportunities for next‐generation biosensors, single‐cell patterning, and lab‐on‐a‐chip devices.
A novel platform to tune the stiffness of a biocompatible polymeric interface up to values characteristic of the bone tissue (GPa range) using ultraviolet (UV) cross‐linking is reported. Since cell growth and differentiation are here controlled solely by the UV‐tunable stiffness of the polymer, this platform is ideal for the simple and rapid fabrication of stiffness patterns and gradients for various biomedical applications. |
| doi_str_mv | 10.1002/adhm.202201503 |
| format | Article |
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A novel platform to tune the stiffness of a biocompatible polymeric interface up to values characteristic of the bone tissue (GPa range) using ultraviolet (UV) cross‐linking is reported. Since cell growth and differentiation are here controlled solely by the UV‐tunable stiffness of the polymer, this platform is ideal for the simple and rapid fabrication of stiffness patterns and gradients for various biomedical applications.</description><identifier>ISSN: 2192-2640</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.202201503</identifier><identifier>PMID: 36565136</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Biocompatibility ; biomaterials ; biomimicry ; Biosensors ; Bone and Bones ; Bone Matrix ; bones ; Cell adhesion ; Cell Differentiation ; Combinatorial analysis ; Differentiation (biology) ; extracellular matrices ; Human tissues ; Humans ; induced mesenchymal stem cells ; Interfaces ; Lab-on-a-chip ; Light irradiation ; Mechanical properties ; Mesenchymal Stem Cells ; Osteogenesis ; Pattern formation ; Polymer films ; Polymers ; Stem cells ; Stiffness ; stiffnesses ; Synergistic effect ; Thin films ; Tuning ; Ultraviolet radiation</subject><ispartof>Advanced healthcare materials, 2023-04, Vol.12 (10), p.e2201503-n/a</ispartof><rights>2023 Wiley‐VCH GmbH</rights><rights>2023 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3953-36ca9678a1a2f5f70baa05f7d32493a4854e464ab74accb4b4938b42829e9a213</cites><orcidid>0000-0002-2423-8801 ; 0000-0002-3797-4971 ; 0000000237974971 ; 0000000224238801</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadhm.202201503$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadhm.202201503$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36565136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1983369$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Zanut, Alessandra</creatorcontrib><creatorcontrib>Li, Rui</creatorcontrib><creatorcontrib>Deng, Ru</creatorcontrib><creatorcontrib>Liu, Xiangyu</creatorcontrib><creatorcontrib>Rejhon, Martin</creatorcontrib><creatorcontrib>Chen, Weiqiang</creatorcontrib><creatorcontrib>Weck, Marcus</creatorcontrib><creatorcontrib>de Peppo, Giuseppe Maria</creatorcontrib><creatorcontrib>Riedo, Elisa</creatorcontrib><title>A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response</title><title>Advanced healthcare materials</title><addtitle>Adv Healthc Mater</addtitle><description>Reproducing in vitro the complex multiscale physical features of human tissues creates novel biomedical opportunities and fundamental understanding of cell−environment interfaces and interactions. While stiffness has been recognized as a key driver of cell behavior, systematic studies on the role of stiffness have been limited to values in the KPa−MPa range, significantly below the stiffness of bone. Here, a platform enabling the tuning of the stiffness of a biocompatible polymeric interface up to values characteristic of human bone is reported, which are in the GPa range, by using extremely thin polymer films on glass and cross‐linking the films using ultraviolet (UV) light irradiation. It is shown that a higher stiffness is related to better adhesion, proliferation, and osteogenic differentiation, and that it is possible to switch on/off cell attachment and growth by solely tuning the stiffness of the interface, without any surface chemistry or topography modification. Since the stiffness is tuned directly by UV irradiation, this platform is ideal for rapid and simple fabrication of stiffness patterns and gradients, thus representing an innovative tool for combinatorial studies of the synergistic effect of tissue environmental cues on cell behavior, and creates new opportunities for next‐generation biosensors, single‐cell patterning, and lab‐on‐a‐chip devices.
A novel platform to tune the stiffness of a biocompatible polymeric interface up to values characteristic of the bone tissue (GPa range) using ultraviolet (UV) cross‐linking is reported. Since cell growth and differentiation are here controlled solely by the UV‐tunable stiffness of the polymer, this platform is ideal for the simple and rapid fabrication of stiffness patterns and gradients for various biomedical applications.</description><subject>Biocompatibility</subject><subject>biomaterials</subject><subject>biomimicry</subject><subject>Biosensors</subject><subject>Bone and Bones</subject><subject>Bone Matrix</subject><subject>bones</subject><subject>Cell adhesion</subject><subject>Cell Differentiation</subject><subject>Combinatorial analysis</subject><subject>Differentiation (biology)</subject><subject>extracellular matrices</subject><subject>Human tissues</subject><subject>Humans</subject><subject>induced mesenchymal stem cells</subject><subject>Interfaces</subject><subject>Lab-on-a-chip</subject><subject>Light irradiation</subject><subject>Mechanical properties</subject><subject>Mesenchymal Stem Cells</subject><subject>Osteogenesis</subject><subject>Pattern formation</subject><subject>Polymer films</subject><subject>Polymers</subject><subject>Stem cells</subject><subject>Stiffness</subject><subject>stiffnesses</subject><subject>Synergistic effect</subject><subject>Thin films</subject><subject>Tuning</subject><subject>Ultraviolet radiation</subject><issn>2192-2640</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuP0zAQhy0EYlfLXjkiCy5cWvyKax9LeCzSViAeZ8txJkqqxC62w5L_HpcuReKCLzOa-fzJ1g-hp5SsKSHslW37ac0IY4RWhD9Al4xqtmKy0g_PvSAX6DqlPSlHVlQq-hhdcFmVnstLtN_iT2FcJoi4tv6HTfhuyD3OPeAveeg6Dynh0P0evA4e8M7mOPzEOZT93C7Y-hbXwecYRryDBN71y2THsoUJ1zCO-DOkQ_AJnqBHnR0TXN_XK_Tt3duv9c3q9uP7D_X2duW4rviKS2e13ChLLeuqbkMaa0mpLWdCcytUJUBIYZuNsM41oilT1QimmAZtGeVX6PnJG1IeTHJDBte74D24bKhWnEtdoJcn6BDD9xlSNtOQXHmu9RDmZNimUpRWTB99L_5B92GOvnzBMEWoUpILXqj1iXIxpBShM4c4TDYuhhJzTMsc0zLntMqFZ_fauZmgPeN_simAPgF3wwjLf3Rm--Zm91f-C3LennU</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Zanut, Alessandra</creator><creator>Li, Rui</creator><creator>Deng, Ru</creator><creator>Liu, Xiangyu</creator><creator>Rejhon, Martin</creator><creator>Chen, Weiqiang</creator><creator>Weck, Marcus</creator><creator>de Peppo, Giuseppe Maria</creator><creator>Riedo, Elisa</creator><general>Wiley Subscription Services, Inc</general><general>Wiley Blackwell (John Wiley & Sons)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T5</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7TO</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0002-2423-8801</orcidid><orcidid>https://orcid.org/0000-0002-3797-4971</orcidid><orcidid>https://orcid.org/0000000237974971</orcidid><orcidid>https://orcid.org/0000000224238801</orcidid></search><sort><creationdate>20230401</creationdate><title>A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response</title><author>Zanut, Alessandra ; Li, Rui ; Deng, Ru ; Liu, Xiangyu ; Rejhon, Martin ; Chen, Weiqiang ; Weck, Marcus ; de Peppo, Giuseppe Maria ; Riedo, Elisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3953-36ca9678a1a2f5f70baa05f7d32493a4854e464ab74accb4b4938b42829e9a213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biocompatibility</topic><topic>biomaterials</topic><topic>biomimicry</topic><topic>Biosensors</topic><topic>Bone and Bones</topic><topic>Bone Matrix</topic><topic>bones</topic><topic>Cell adhesion</topic><topic>Cell Differentiation</topic><topic>Combinatorial analysis</topic><topic>Differentiation (biology)</topic><topic>extracellular matrices</topic><topic>Human tissues</topic><topic>Humans</topic><topic>induced mesenchymal stem cells</topic><topic>Interfaces</topic><topic>Lab-on-a-chip</topic><topic>Light irradiation</topic><topic>Mechanical properties</topic><topic>Mesenchymal Stem Cells</topic><topic>Osteogenesis</topic><topic>Pattern formation</topic><topic>Polymer films</topic><topic>Polymers</topic><topic>Stem cells</topic><topic>Stiffness</topic><topic>stiffnesses</topic><topic>Synergistic effect</topic><topic>Thin films</topic><topic>Tuning</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zanut, Alessandra</creatorcontrib><creatorcontrib>Li, Rui</creatorcontrib><creatorcontrib>Deng, Ru</creatorcontrib><creatorcontrib>Liu, Xiangyu</creatorcontrib><creatorcontrib>Rejhon, Martin</creatorcontrib><creatorcontrib>Chen, Weiqiang</creatorcontrib><creatorcontrib>Weck, Marcus</creatorcontrib><creatorcontrib>de Peppo, Giuseppe Maria</creatorcontrib><creatorcontrib>Riedo, Elisa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Immunology Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Advanced healthcare materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zanut, Alessandra</au><au>Li, Rui</au><au>Deng, Ru</au><au>Liu, Xiangyu</au><au>Rejhon, Martin</au><au>Chen, Weiqiang</au><au>Weck, Marcus</au><au>de Peppo, Giuseppe Maria</au><au>Riedo, Elisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response</atitle><jtitle>Advanced healthcare materials</jtitle><addtitle>Adv Healthc Mater</addtitle><date>2023-04-01</date><risdate>2023</risdate><volume>12</volume><issue>10</issue><spage>e2201503</spage><epage>n/a</epage><pages>e2201503-n/a</pages><issn>2192-2640</issn><eissn>2192-2659</eissn><abstract>Reproducing in vitro the complex multiscale physical features of human tissues creates novel biomedical opportunities and fundamental understanding of cell−environment interfaces and interactions. While stiffness has been recognized as a key driver of cell behavior, systematic studies on the role of stiffness have been limited to values in the KPa−MPa range, significantly below the stiffness of bone. Here, a platform enabling the tuning of the stiffness of a biocompatible polymeric interface up to values characteristic of human bone is reported, which are in the GPa range, by using extremely thin polymer films on glass and cross‐linking the films using ultraviolet (UV) light irradiation. It is shown that a higher stiffness is related to better adhesion, proliferation, and osteogenic differentiation, and that it is possible to switch on/off cell attachment and growth by solely tuning the stiffness of the interface, without any surface chemistry or topography modification. Since the stiffness is tuned directly by UV irradiation, this platform is ideal for rapid and simple fabrication of stiffness patterns and gradients, thus representing an innovative tool for combinatorial studies of the synergistic effect of tissue environmental cues on cell behavior, and creates new opportunities for next‐generation biosensors, single‐cell patterning, and lab‐on‐a‐chip devices.
A novel platform to tune the stiffness of a biocompatible polymeric interface up to values characteristic of the bone tissue (GPa range) using ultraviolet (UV) cross‐linking is reported. Since cell growth and differentiation are here controlled solely by the UV‐tunable stiffness of the polymer, this platform is ideal for the simple and rapid fabrication of stiffness patterns and gradients for various biomedical applications.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36565136</pmid><doi>10.1002/adhm.202201503</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2423-8801</orcidid><orcidid>https://orcid.org/0000-0002-3797-4971</orcidid><orcidid>https://orcid.org/0000000237974971</orcidid><orcidid>https://orcid.org/0000000224238801</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biocompatibility biomaterials biomimicry Biosensors Bone and Bones Bone Matrix bones Cell adhesion Cell Differentiation Combinatorial analysis Differentiation (biology) extracellular matrices Human tissues Humans induced mesenchymal stem cells Interfaces Lab-on-a-chip Light irradiation Mechanical properties Mesenchymal Stem Cells Osteogenesis Pattern formation Polymer films Polymers Stem cells Stiffness stiffnesses Synergistic effect Thin films Tuning Ultraviolet radiation |
| title | A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell Response |
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