Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

Abstract Noncanonical redox cofactors are attractive low-cost alternatives to nicotinamide adenine dinucleotide (phosphate) (NAD(P)+) in biotransformation. However, engineering enzymes to utilize them is challenging. Here, we present a high-throughput directed evolution platform which couples cell g...

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Veröffentlicht in:	Nature communications 2022-08, Vol.13 (1)
Hauptverfasser:	Zhang, Linyue, King, Edward, Black, William B., Heckmann, Christian M., Wolder, Allison, Cui, Youtian, Nicklen, Francis, Siegel, Justin B., Luo, Ray, Paul, Caroline E., Li, Han
Format:	Artikel
Sprache:	eng
Schlagworte:	Science & Technology - Other Topics
Online-Zugang:	Volltext
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