In Situ Optical Quantification of Extracellular Electron Transfer Using Plasmonic Metal Oxide Nanocrystals
Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on a variety of inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes. To...
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creator | Graham, Austin J. Gibbs, Stephen L. Saez Cabezas, Camila A. Wang, Yongdan Green, Allison M. Milliron, Delia J. Keitz, Benjamin K. |
description | Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on a variety of inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes. To address this, we developed a platform for quantifying EET flux from cell suspensions using aqueous dispersions of infrared plasmonic tin‐doped indium oxide nanocrystals. Tracking the change in optical extinction during electron transfer enabled quantification of current generated by planktonic Shewanella oneidensis cultures. Using this method, we differentiated between starved and actively respiring cells, cells of varying genotype, and cells engineered to differentially express a key EET gene using an inducible genetic circuit. Overall, our results validate the utility of colloidally stable plasmonic metal oxide nanocrystals as quantitative biosensors in aqueous environments and contribute to a fundamental understanding of planktonic S. oneidensis electrophysiology using simple in situ spectroscopy.
Electroactive bacteria in situ: Colloidally dispersed plasmonic metal oxide nanocrystals optically track extracellular electron transfer from planktonic Shewanella oneidensis cultures. The results indicate that plasmonic semiconductor nanocrystals are a reliable infrared sensing platform for probing metabolic activity of electroactive bacteria in cellular environments. |
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Electroactive bacteria in situ: Colloidally dispersed plasmonic metal oxide nanocrystals optically track extracellular electron transfer from planktonic Shewanella oneidensis cultures. The results indicate that plasmonic semiconductor nanocrystals are a reliable infrared sensing platform for probing metabolic activity of electroactive bacteria in cellular environments.</description><identifier>ISSN: 2196-0216</identifier><identifier>EISSN: 2196-0216</identifier><identifier>DOI: 10.1002/celc.202101423</identifier><language>eng</language><publisher>Weinheim: John Wiley & Sons, Inc</publisher><subject>Aqueous environments ; Biosensors ; Circuits ; Colloidal semiconductor nanocrystals ; Electron transfer ; Electrons ; Electrophysiology ; Extracellular electron transfer ; Indium oxides ; Infrared tracking ; Localized surface plasmon resonance ; Metal oxides ; Microorganisms ; Nanocrystals ; Plasmonics ; Substrates</subject><ispartof>ChemElectroChem, 2022-02, Vol.9 (3), p.n/a</ispartof><rights>2021 Wiley‐VCH GmbH</rights><rights>2022 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3843-95ad602b511d7100b8f53b0df74cafe33cd98c5d2fe7ac7fbbcf5f6da01772a43</citedby><cites>FETCH-LOGICAL-c3843-95ad602b511d7100b8f53b0df74cafe33cd98c5d2fe7ac7fbbcf5f6da01772a43</cites><orcidid>0000-0003-2533-0957 ; 0000-0003-3314-0053 ; 0000-0001-8924-181X ; 0000-0002-8734-0096 ; 0000-0002-8737-451X ; 0000000333140053 ; 0000000287340096 ; 0000000325330957 ; 000000028737451X ; 000000018924181X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcelc.202101423$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcelc.202101423$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.osti.gov/biblio/1843073$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Graham, Austin J.</creatorcontrib><creatorcontrib>Gibbs, Stephen L.</creatorcontrib><creatorcontrib>Saez Cabezas, Camila A.</creatorcontrib><creatorcontrib>Wang, Yongdan</creatorcontrib><creatorcontrib>Green, Allison M.</creatorcontrib><creatorcontrib>Milliron, Delia J.</creatorcontrib><creatorcontrib>Keitz, Benjamin K.</creatorcontrib><title>In Situ Optical Quantification of Extracellular Electron Transfer Using Plasmonic Metal Oxide Nanocrystals</title><title>ChemElectroChem</title><description>Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on a variety of inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes. To address this, we developed a platform for quantifying EET flux from cell suspensions using aqueous dispersions of infrared plasmonic tin‐doped indium oxide nanocrystals. Tracking the change in optical extinction during electron transfer enabled quantification of current generated by planktonic Shewanella oneidensis cultures. Using this method, we differentiated between starved and actively respiring cells, cells of varying genotype, and cells engineered to differentially express a key EET gene using an inducible genetic circuit. Overall, our results validate the utility of colloidally stable plasmonic metal oxide nanocrystals as quantitative biosensors in aqueous environments and contribute to a fundamental understanding of planktonic S. oneidensis electrophysiology using simple in situ spectroscopy.
Electroactive bacteria in situ: Colloidally dispersed plasmonic metal oxide nanocrystals optically track extracellular electron transfer from planktonic Shewanella oneidensis cultures. The results indicate that plasmonic semiconductor nanocrystals are a reliable infrared sensing platform for probing metabolic activity of electroactive bacteria in cellular environments.</description><subject>Aqueous environments</subject><subject>Biosensors</subject><subject>Circuits</subject><subject>Colloidal semiconductor nanocrystals</subject><subject>Electron transfer</subject><subject>Electrons</subject><subject>Electrophysiology</subject><subject>Extracellular electron transfer</subject><subject>Indium oxides</subject><subject>Infrared tracking</subject><subject>Localized surface plasmon resonance</subject><subject>Metal oxides</subject><subject>Microorganisms</subject><subject>Nanocrystals</subject><subject>Plasmonics</subject><subject>Substrates</subject><issn>2196-0216</issn><issn>2196-0216</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkM9PwyAUxxujicvc1TPRcyc_2tIezVJ1yXQatzOhFJSlgwk0bv-9LDXqzdN7wOf7fY9vklwiOEUQ4hshOzHFECOIMkxOkhFGVZHGc3H6pz9PJt5vIIQIwZyUxSjZzA141aEHy13QgnfgpecmaBX7oK0BVoF6HxyP_l3fcQfqTorg4svKceOVdGDttXkDzx33W2u0AI8yRJ_lXrcSPHFjhTv4eOMvkjMVi5x813GyvqtXs4d0sbyfz24XqSBlRtIq520BcZMj1NL4t6ZUOWlgq2gmuJKEiLYqRd5iJSkXVDWNULkqWg4RpZhnZJxcDb7WB8280EGKd2GNiYszFEdASiJ0PUA7Zz966QPb2N6ZuBfDBa4IohDjSE0HSjjrvZOK7ZzecndgCLJj7uyYO_vJPQqqQfCpO3n4h2azejH71X4BfpWHog</recordid><startdate>20220211</startdate><enddate>20220211</enddate><creator>Graham, Austin J.</creator><creator>Gibbs, Stephen L.</creator><creator>Saez Cabezas, Camila A.</creator><creator>Wang, Yongdan</creator><creator>Green, Allison M.</creator><creator>Milliron, Delia J.</creator><creator>Keitz, Benjamin K.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Blackwell (John Wiley & Sons)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0003-2533-0957</orcidid><orcidid>https://orcid.org/0000-0003-3314-0053</orcidid><orcidid>https://orcid.org/0000-0001-8924-181X</orcidid><orcidid>https://orcid.org/0000-0002-8734-0096</orcidid><orcidid>https://orcid.org/0000-0002-8737-451X</orcidid><orcidid>https://orcid.org/0000000333140053</orcidid><orcidid>https://orcid.org/0000000287340096</orcidid><orcidid>https://orcid.org/0000000325330957</orcidid><orcidid>https://orcid.org/000000028737451X</orcidid><orcidid>https://orcid.org/000000018924181X</orcidid></search><sort><creationdate>20220211</creationdate><title>In Situ Optical Quantification of Extracellular Electron Transfer Using Plasmonic Metal Oxide Nanocrystals</title><author>Graham, Austin J. ; 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However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes. To address this, we developed a platform for quantifying EET flux from cell suspensions using aqueous dispersions of infrared plasmonic tin‐doped indium oxide nanocrystals. Tracking the change in optical extinction during electron transfer enabled quantification of current generated by planktonic Shewanella oneidensis cultures. Using this method, we differentiated between starved and actively respiring cells, cells of varying genotype, and cells engineered to differentially express a key EET gene using an inducible genetic circuit. Overall, our results validate the utility of colloidally stable plasmonic metal oxide nanocrystals as quantitative biosensors in aqueous environments and contribute to a fundamental understanding of planktonic S. oneidensis electrophysiology using simple in situ spectroscopy.
Electroactive bacteria in situ: Colloidally dispersed plasmonic metal oxide nanocrystals optically track extracellular electron transfer from planktonic Shewanella oneidensis cultures. The results indicate that plasmonic semiconductor nanocrystals are a reliable infrared sensing platform for probing metabolic activity of electroactive bacteria in cellular environments.</abstract><cop>Weinheim</cop><pub>John Wiley & Sons, Inc</pub><doi>10.1002/celc.202101423</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2533-0957</orcidid><orcidid>https://orcid.org/0000-0003-3314-0053</orcidid><orcidid>https://orcid.org/0000-0001-8924-181X</orcidid><orcidid>https://orcid.org/0000-0002-8734-0096</orcidid><orcidid>https://orcid.org/0000-0002-8737-451X</orcidid><orcidid>https://orcid.org/0000000333140053</orcidid><orcidid>https://orcid.org/0000000287340096</orcidid><orcidid>https://orcid.org/0000000325330957</orcidid><orcidid>https://orcid.org/000000028737451X</orcidid><orcidid>https://orcid.org/000000018924181X</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Aqueous environments Biosensors Circuits Colloidal semiconductor nanocrystals Electron transfer Electrons Electrophysiology Extracellular electron transfer Indium oxides Infrared tracking Localized surface plasmon resonance Metal oxides Microorganisms Nanocrystals Plasmonics Substrates |
title | In Situ Optical Quantification of Extracellular Electron Transfer Using Plasmonic Metal Oxide Nanocrystals |
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