Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli

We report that two-stage dynamic control improves bioprocess robustness as a result of the dynamic deregulation of central metabolism. Dynamic control is implemented during stationary phase using combinations of CRISPR interference and controlled proteolysis to reduce levels of central metabolic enz...

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Veröffentlicht in:	Metabolic engineering 2021-11, Vol.68 (C), p.106-118
Hauptverfasser:	Ye, Zhixia, Li, Shuai, Hennigan, Jennifer N., Lebeau, Juliana, Moreb, Eirik A., Wolf, Jacob, Lynch, Michael D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Controlled proteolysis CRISPR Dynamic metabolic control High-throughput metabolic engineering Metabolic valves Process robustness Scalability Standardization Two-stage
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