Probing specificities of alcohol acyltransferases for designer ester biosynthesis with a high‐throughput microbial screening platform

Alcohol acyltransferases (AATs) enables microbial biosynthesis of a large space of esters by condensing an alcohol and an acyl‐CoA. However, substrate promiscuity of AATs prevents microbial biosynthesis of designer esters with high selectivity. Here, we developed a high‐throughput microbial screenin...

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Veröffentlicht in:	Biotechnology and bioengineering 2021-12, Vol.118 (12), p.4655-4667
Hauptverfasser:	Lee, Jong‐Won, Seo, Hyeongmin, Young, Caleb, Trinh, Cong T.
Format:	Artikel
Sprache:	eng
Schlagworte:	2‐phenhylethyl acetate AAT Acetic acid Acetyltransferase Acyltransferases - chemistry Acyltransferases - genetics Acyltransferases - metabolism Alcohol alcohol acetyltransferase Alcohols Biosynthesis CAT Catalysis Chloramphenicol chloramphenicol acetyltransferase Chloramphenicol O-acetyltransferase Chloromycetin colorimetric assay Colorimetry Culture techniques Escherichia coli Escherichia coli - genetics Esters Esters - metabolism ethyl acetate Fermentation High-Throughput Screening Assays - methods High‐throughput microbial screening isobutyl acetate Microorganisms model‐guided protein design Molecular Docking Simulation Mutation n‐butyl acetate Protein Engineering - methods Proteins - chemistry Proteins - genetics Proteins - metabolism Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Screening Selectivity solvent overlays Substrate specificity Substrates
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